Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11k22r
(732 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g27470.1 68418.m03281 seryl-tRNA synthetase / serine--tRNA li... 58 5e-09
At1g11870.2 68414.m01369 seryl-tRNA synthetase, putative / serin... 50 2e-06
At1g11870.1 68414.m01368 seryl-tRNA synthetase, putative / serin... 50 2e-06
At4g10180.1 68417.m01668 light-mediated development protein 1 / ... 31 1.0
At1g47915.1 68414.m05336 F-box family protein contains F-box dom... 29 3.2
At1g60360.1 68414.m06796 zinc finger (C3HC4-type RING finger) fa... 28 5.6
At1g56430.1 68414.m06490 nicotianamine synthase, putative simila... 28 5.6
At1g48400.1 68414.m05406 F-box family protein contains F-box dom... 28 5.6
At5g44750.1 68418.m05485 UMUC-like DNA repair family protein / B... 28 7.3
At5g08110.1 68418.m00946 DEAD/DEAH box helicase, putative severa... 28 7.3
At4g26630.1 68417.m03837 expressed protein 28 7.3
At1g77730.1 68414.m09050 pleckstrin homology (PH) domain-contain... 28 7.3
At3g63530.1 68416.m07156 zinc finger (C3HC4-type RING finger) fa... 27 9.7
>At5g27470.1 68418.m03281 seryl-tRNA synthetase / serine--tRNA
ligase identical to SP|Q39230 Seryl-tRNA synthetase (EC
6.1.1.11) (Serine--tRNA ligase) (SerRS) {Arabidopsis
thaliana}
Length = 451
Score = 58.4 bits (135), Expect = 5e-09
Identities = 48/197 (24%), Positives = 89/197 (45%), Gaps = 12/197 (6%)
Frame = -1
Query: 639 DSRLHLTGGGSLCSYMAYHAKNVLYPKVLPLKYFNMGRQYVPVAS----DFNSLLTVSQA 472
D L T LC AYH ++P LPL+Y + A D + V Q
Sbjct: 232 DKYLIATAEQPLC---AYHIDEWIHPTELPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQF 288
Query: 471 AVVEFFAVTKAATDID-EMLDNLIEILKQYYSRFDYHFRISMTAAHQLNMWESLRLSVEM 295
+E F +T + EMLD +++ + +Y ++I + LN + + +E
Sbjct: 289 EKIEQFCITGPNENASWEMLDEMMKNSEDFYQALKLPYQIVSIVSGALNDAAAKKYDLEA 348
Query: 294 YSSSQQKYATIANVSLSGDFISKRLMFTY---VDNKVTK-FPHILSGTLLNVPKFLACVL 127
+ S + + + + S D+ ++RL Y N+ TK + H+L+ TL + + C+L
Sbjct: 349 WFPSSETFRELVSCSNCTDYQARRLEIRYGQKKSNEQTKQYVHMLNSTLTATERTICCIL 408
Query: 126 E---QDNDFCVPDILKP 85
E +++ +P++L+P
Sbjct: 409 ENYQREDGVDIPEVLQP 425
>At1g11870.2 68414.m01369 seryl-tRNA synthetase, putative /
serine--tRNA ligase, putative similar to PIR|T03949
serine--tRNA ligase (EC 6.1.1.11) serS {Zea mays};
contains Pfam profiles PF00587: tRNA synthetase class II
core domain (G, H, P, S and T), PF02403: Seryl-tRNA
synthetase N-terminal domain
Length = 514
Score = 49.6 bits (113), Expect = 2e-06
Identities = 44/189 (23%), Positives = 76/189 (40%), Gaps = 22/189 (11%)
Frame = -1
Query: 585 HAKNVLYPKVLPLKYFNMGRQYVPVASDFNS----LLTVSQAAVVEFFAVTKAATDIDEM 418
H ++L LPLKY + A + L V Q + E F + + D +
Sbjct: 314 HMDSILLESALPLKYIAFSHCFRTEAGAAGAATKGLYRVHQFSKAEMFVICQPE-DSESF 372
Query: 417 LDNLIEILKQYYSRFDYHFRISMTAAHQLNMWESLRLSVEMYSSSQQKYATIANVSLSGD 238
LI+I + ++ HF+ A L + +E + ++ I++ S D
Sbjct: 373 HQELIQIEEDLFTSLGLHFKTLDMATADLGAPAYRKFDIEAWMPGLGRFGEISSASNCTD 432
Query: 237 FISKRLMFTYVDNK--------------VTKFPHILSGTLLNVPKFLACVLE----QDND 112
+ S+RL Y ++ TKF H L+ T VP+ + C+LE +D
Sbjct: 433 YQSRRLGIRYRPSEPPQTGPKKGKANLPATKFVHTLNATACAVPRMMVCLLENYQQEDGS 492
Query: 111 FCVPDILKP 85
+P+ L+P
Sbjct: 493 VVIPEPLRP 501
>At1g11870.1 68414.m01368 seryl-tRNA synthetase, putative /
serine--tRNA ligase, putative similar to PIR|T03949
serine--tRNA ligase (EC 6.1.1.11) serS {Zea mays};
contains Pfam profiles PF00587: tRNA synthetase class II
core domain (G, H, P, S and T), PF02403: Seryl-tRNA
synthetase N-terminal domain
Length = 512
Score = 49.6 bits (113), Expect = 2e-06
Identities = 44/189 (23%), Positives = 76/189 (40%), Gaps = 22/189 (11%)
Frame = -1
Query: 585 HAKNVLYPKVLPLKYFNMGRQYVPVASDFNS----LLTVSQAAVVEFFAVTKAATDIDEM 418
H ++L LPLKY + A + L V Q + E F + + D +
Sbjct: 312 HMDSILLESALPLKYIAFSHCFRTEAGAAGAATKGLYRVHQFSKAEMFVICQPE-DSESF 370
Query: 417 LDNLIEILKQYYSRFDYHFRISMTAAHQLNMWESLRLSVEMYSSSQQKYATIANVSLSGD 238
LI+I + ++ HF+ A L + +E + ++ I++ S D
Sbjct: 371 HQELIQIEEDLFTSLGLHFKTLDMATADLGAPAYRKFDIEAWMPGLGRFGEISSASNCTD 430
Query: 237 FISKRLMFTYVDNK--------------VTKFPHILSGTLLNVPKFLACVLE----QDND 112
+ S+RL Y ++ TKF H L+ T VP+ + C+LE +D
Sbjct: 431 YQSRRLGIRYRPSEPPQTGPKKGKANLPATKFVHTLNATACAVPRMMVCLLENYQQEDGS 490
Query: 111 FCVPDILKP 85
+P+ L+P
Sbjct: 491 VVIPEPLRP 499
>At4g10180.1 68417.m01668 light-mediated development protein 1 /
deetiolated1 (DET1) identical to Light-mediated
development protein DET1 (Deetiolated1)
(Swiss-Prot:P48732) [Arabidopsis thaliana]
Length = 543
Score = 30.7 bits (66), Expect = 1.0
Identities = 27/82 (32%), Positives = 37/82 (45%), Gaps = 1/82 (1%)
Frame = -1
Query: 459 FFAV-TKAATDIDEMLDNLIEILKQYYSRFDYHFRISMTAAHQLNMWESLRLSVEMYSSS 283
FFAV TDI N E L Q + +F HF +S + +N S S +Y+
Sbjct: 362 FFAVYNMETTDIVAFYQNSAEDLYQLFEQFSDHFTVSSSTPF-MNFVTS--HSNNVYALE 418
Query: 282 QQKYATIANVSLSGDFISKRLM 217
Q KY + S S F+ K L+
Sbjct: 419 QLKYTKNKSNSFS-QFVKKMLL 439
>At1g47915.1 68414.m05336 F-box family protein contains F-box domain
Pfam:PF00646 ; similar to SKP1 interacting partner 2
(SKIP2) TIGR_Ath1:At5g67250
Length = 73
Score = 29.1 bits (62), Expect = 3.2
Identities = 13/57 (22%), Positives = 29/57 (50%)
Frame = +2
Query: 152 TFNNVPDRICGNFVTLLSTYVNISLLLMKSPLKDTFAMVAYFCWELEYISTDNLKDS 322
+ +N+PD I G ++LL T V S ++ ++ +V C++ + N +++
Sbjct: 9 SISNLPDEILGKILSLLPTKVAASTSVLSKRWRNLLGLVDNLCFDESVVVYPNKEEA 65
>At1g60360.1 68414.m06796 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 327
Score = 28.3 bits (60), Expect = 5.6
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -3
Query: 310 VVCGNVFQLPTKICYHSKCILKW 242
+V G+ +LP K YH CI+ W
Sbjct: 232 IVGGDATELPCKHIYHKDCIVPW 254
>At1g56430.1 68414.m06490 nicotianamine synthase, putative similar
to nicotianamine synthase [Lycopersicon
esculentum][GI:4753801], nicotianamine synthase 2
[Hordeum vulgare][GI:4894912]
Length = 324
Score = 28.3 bits (60), Expect = 5.6
Identities = 16/48 (33%), Positives = 26/48 (54%), Gaps = 5/48 (10%)
Frame = -1
Query: 450 VTKAATDIDEMLDNLIEILKQYYSRFDYHFRISMTA-----AHQLNMW 322
VTK + +I EM NLI+I + ++HF +T+ H LN++
Sbjct: 52 VTKMSENIQEMRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLF 99
>At1g48400.1 68414.m05406 F-box family protein contains F-box domain
Pfam:PF00646
Length = 513
Score = 28.3 bits (60), Expect = 5.6
Identities = 12/45 (26%), Positives = 24/45 (53%)
Frame = +2
Query: 152 TFNNVPDRICGNFVTLLSTYVNISLLLMKSPLKDTFAMVAYFCWE 286
+ +N+PD I G ++LL T V S ++ ++ +V C++
Sbjct: 11 SISNLPDEILGKILSLLPTKVAASTSVLSKRWRNLLGLVDNLCFD 55
>At5g44750.1 68418.m05485 UMUC-like DNA repair family protein / BRCT
domain-containing protein low similarity to Rev1S [Homo
sapiens] GI:12483635; contains Pfam profiles PF00817:
ImpB/MucB/SamB family, PF00533: BRCA1 C Terminus (BRCT)
domain
Length = 1102
Score = 27.9 bits (59), Expect = 7.3
Identities = 17/48 (35%), Positives = 26/48 (54%), Gaps = 2/48 (4%)
Frame = -1
Query: 522 YVPVASDFNSLLTVSQAAVVEF--FAVTKAATDIDEMLDNLIEILKQY 385
Y VA L V Q A+ E F +A+D+D+ + ++ E+LKQY
Sbjct: 1000 YFKVAQSRPMLSLVLQHAISEMSSFPDAASASDLDKAIYDVCELLKQY 1047
>At5g08110.1 68418.m00946 DEAD/DEAH box helicase, putative several
putative ATP-dependent helicases
Length = 1058
Score = 27.9 bits (59), Expect = 7.3
Identities = 11/39 (28%), Positives = 20/39 (51%)
Frame = -1
Query: 543 YFNMGRQYVPVASDFNSLLTVSQAAVVEFFAVTKAATDI 427
Y N GR Y+ + D + + + V+++ T+ TDI
Sbjct: 821 YMNQGRNYLVTSLDIKEKVALCELVNVDYYTRTRDYTDI 859
>At4g26630.1 68417.m03837 expressed protein
Length = 763
Score = 27.9 bits (59), Expect = 7.3
Identities = 10/32 (31%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
Frame = +1
Query: 154 IQQCT*QNMWKFCNFI-IHICKHQPLTNEVTT 246
+++CT + +W+FC+ + IHI K ++ T
Sbjct: 415 LEKCTKEKLWEFCDVLDIHITKATTKKEDIIT 446
>At1g77730.1 68414.m09050 pleckstrin homology (PH) domain-containing
protein similar to SP|P16258 Oxysterol-binding protein 1
{Oryctolagus cuniculus}; contains Pfam profile PF00169:
PH domain
Length = 265
Score = 27.9 bits (59), Expect = 7.3
Identities = 19/62 (30%), Positives = 34/62 (54%), Gaps = 2/62 (3%)
Frame = -1
Query: 366 HFRISMTAAHQLNMWESLRLSVEMYS--SSQQKYATIANVSLSGDFISKRLMFTYVDNKV 193
H R + T+ + + E+L+ E + S+++ A+ NVS+S D + +RLM VD +
Sbjct: 158 HLRAA-TSEDRASWIEALKAVKETFPRMSNEELMASTTNVSVSTDKLRQRLMEEEVDETI 216
Query: 192 TK 187
K
Sbjct: 217 IK 218
>At3g63530.1 68416.m07156 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 248
Score = 27.5 bits (58), Expect = 9.7
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = -3
Query: 286 LPTKICYHSKCILKW 242
LP K YHS+CI KW
Sbjct: 213 LPCKHVYHSECISKW 227
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,793,093
Number of Sequences: 28952
Number of extensions: 331354
Number of successful extensions: 879
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 857
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 878
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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