BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11k21f (643 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 226 2e-60 SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 196 2e-51 SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 98 8e-22 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.11 SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 31 0.14 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.00 SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces pombe... 28 1.3 SPBC36.09 |sap61||U2 snRNP-associated protein sap61|Schizosaccha... 27 1.7 SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr ... 27 1.7 SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 2.3 SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating tra... 27 3.0 SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyce... 26 4.0 SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |S... 25 7.0 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 7.0 SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large... 25 9.3 SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 25 9.3 >SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces pombe|chr 1|||Manual Length = 161 Score = 226 bits (552), Expect = 2e-60 Identities = 111/147 (75%), Positives = 130/147 (88%) Frame = +1 Query: 127 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 306 P+Y PFFGVMG +AI+F++ GAAYGTAK+G GI+AM V+RP+LI+K+ IPVVMAGIIAI Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAI 66 Query: 307 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQP 486 YGLVV+VLI+G L++ + LY GFI LGAGL+VG +GLAAGFAIGIVGDAGVRGTAQQP Sbjct: 67 YGLVVSVLISGNLKQILS--LYSGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQP 124 Query: 487 RLFVGMILILIFAEVLGLYGLIVAIYL 567 RLFV MILILIFAEVLGLYGLIVA+ L Sbjct: 125 RLFVAMILILIFAEVLGLYGLIVALLL 151 >SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 162 Score = 196 bits (479), Expect = 2e-51 Identities = 91/147 (61%), Positives = 116/147 (78%) Frame = +1 Query: 127 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 306 PIY FFG G ++++FS LGA YGTA +G GIAA+ RPE++MKS+IPVVM+GII + Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66 Query: 307 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQP 486 YGLV++VLIAG + +Y L+ GFIHL AGLAVG +G+AAG+AIG+VGD GV+ +Q Sbjct: 67 YGLVMSVLIAGDMSPDNDYSLFSGFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQSFMRQD 126 Query: 487 RLFVGMILILIFAEVLGLYGLIVAIYL 567 R+FV M+LILIFAEVLGLYGLIV + L Sbjct: 127 RIFVSMVLILIFAEVLGLYGLIVGLIL 153 >SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces pombe|chr 1|||Manual Length = 199 Score = 98.3 bits (234), Expect = 8e-22 Identities = 49/147 (33%), Positives = 81/147 (55%), Gaps = 6/147 (4%) Frame = +1 Query: 145 FGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVA 324 +G++G AS + F +GAA+G GT I +V P + K++I ++ ++AIY L++A Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIA 104 Query: 325 VLIAGALQE--PANY----PLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQP 486 ++ + + + PA + Y GF G+ VG L G +GI G + AQ Sbjct: 105 IVFSAKINDINPAGFYTKSHYYTGFALFWGGITVGLCNLICGVCVGITGSSAALADAQDA 164 Query: 487 RLFVGMILILIFAEVLGLYGLIVAIYL 567 LFV ++++ IF VLGL+GLIV + + Sbjct: 165 SLFVKVLVVEIFGSVLGLFGLIVGLLI 191 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 31.5 bits (68), Expect = 0.11 Identities = 20/57 (35%), Positives = 28/57 (49%) Frame = -3 Query: 545 P*RPNTSAKIRIRIIPTNNLGC*AVPRTPASPTMPMAKPAARPENPTAKPAPKWMNP 375 P RP ++A ++ PT +VP P++P MP P+A P A AP NP Sbjct: 1691 PVRPQSAAPPQMSA-PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746 >SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 288 Score = 31.1 bits (67), Expect = 0.14 Identities = 21/68 (30%), Positives = 30/68 (44%) Frame = +1 Query: 271 IIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIV 450 II +AG+IA + +++ IAG + G ++ G L LA GF I Sbjct: 193 IITATIAGVIAAFSVIITATIAGVIAAMV------GILYFGHWLVYKILILAFGFKIVTS 246 Query: 451 GDAGVRGT 474 GD V T Sbjct: 247 GDVCVSNT 254 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 28.3 bits (60), Expect = 1.00 Identities = 26/65 (40%), Positives = 30/65 (46%), Gaps = 2/65 (3%) Frame = -3 Query: 476 AVPRTPAS--PTMPMAKPAARPENPTAKPAPKWMNPL*RG*LAGSWRAPAIRTATTRP*M 303 A P P S P++PM PAA P P+A AP PL AG AP + A P Sbjct: 427 APPSLPPSAPPSLPMGAPAAPPLPPSAPIAP----PL----PAGMPAAPPLPPAAPAPPP 478 Query: 302 AIIPA 288 A PA Sbjct: 479 APAPA 483 >SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 281 Score = 27.9 bits (59), Expect = 1.3 Identities = 17/39 (43%), Positives = 25/39 (64%), Gaps = 3/39 (7%) Frame = -2 Query: 573 CVQVDGDD-KSV--KTQYFSENKNKNHSDE*PRLLSSTT 466 C++VD +D K + K+QY +EN N N + P L S+TT Sbjct: 233 CIEVDSEDWKDLVWKSQYATENANTNSINNSP-LSSNTT 270 >SPBC36.09 |sap61||U2 snRNP-associated protein sap61|Schizosaccharomyces pombe|chr 2|||Manual Length = 492 Score = 27.5 bits (58), Expect = 1.7 Identities = 13/44 (29%), Positives = 25/44 (56%) Frame = +3 Query: 492 IRRNDSYSYFR*SIGSLRTYRRHLPVHKINALNTHHSRRPCSEP 623 I +D + F S+G ++ + + P HK+ L+ +S +P S+P Sbjct: 76 INADDDLTEFYKSLGEIQEFHKKYPDHKVEDLSQLYSIKP-SQP 118 >SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr 1|||Manual Length = 249 Score = 27.5 bits (58), Expect = 1.7 Identities = 22/72 (30%), Positives = 32/72 (44%) Frame = +1 Query: 130 IYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIY 309 +YGPF+ A+ FS Y +G G + S+ K +I A II Y Sbjct: 87 LYGPFWITTTVIQALFFSNSITEYARYATGHGTSGYSI-------KKLISA--ASIIYGY 137 Query: 310 GLVVAVLIAGAL 345 ++AVL+ G L Sbjct: 138 TTIIAVLLWGIL 149 >SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1729 Score = 27.1 bits (57), Expect = 2.3 Identities = 21/95 (22%), Positives = 47/95 (49%), Gaps = 6/95 (6%) Frame = +1 Query: 106 NKMAENNP---IYGPFFG-VMGAASAIIFSALGAAYGTAKSGTGIAAMSVMR-PELIMKS 270 +K+A ++P I F+G ++G + F+ + + ++ G S LI+ S Sbjct: 1424 DKIAMDSPRARITTMFYGEILGPLGTLFFTCIPFLFINSQPGNDDETQSTNAFIRLIIMS 1483 Query: 271 IIPVVMAGIIAIYGLVVAVLIAGALQEPA-NYPLY 372 + P+V++ IIA + + +++ L + + Y +Y Sbjct: 1484 VAPLVLSAIIAFFFFCLGIMLRPILGDRSKTYGVY 1518 >SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating transcription Rct1|Schizosaccharomyces pombe|chr 2|||Manual Length = 432 Score = 26.6 bits (56), Expect = 3.0 Identities = 10/23 (43%), Positives = 14/23 (60%) Frame = -1 Query: 85 TGPKNCDDYLHTLTPRNYFCTDK 17 TG KNCDD+L +P + D+ Sbjct: 402 TGNKNCDDHLRDKSPERRYRYDR 424 >SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyces pombe|chr 2|||Manual Length = 760 Score = 26.2 bits (55), Expect = 4.0 Identities = 14/38 (36%), Positives = 22/38 (57%) Frame = -2 Query: 384 DEPFVKGVVGWLLEGTSNQDSHDQTVDGNNTRHDDRND 271 DEPF+K +L+ SN++S+ VD N+ D +D Sbjct: 48 DEPFLKSKYMDILQKISNRESNVINVDLNDLYEFDPSD 85 >SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 25.4 bits (53), Expect = 7.0 Identities = 8/13 (61%), Positives = 10/13 (76%) Frame = -1 Query: 85 TGPKNCDDYLHTL 47 TGP N DDY+H + Sbjct: 369 TGPSNTDDYIHRI 381 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 25.4 bits (53), Expect = 7.0 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 4/34 (11%) Frame = -3 Query: 476 AVPRTPASPTMPMA----KPAARPENPTAKPAPK 387 +VP+ PA+P +P A +P A P P AP+ Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQ 546 >SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1405 Score = 25.0 bits (52), Expect = 9.3 Identities = 8/19 (42%), Positives = 13/19 (68%) Frame = -1 Query: 451 PRCLWRNRQPDQRILQPNQ 395 PRCLW +Q +++PN+ Sbjct: 582 PRCLWTGKQVFTVLMKPNR 600 >SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit Bgs2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1894 Score = 25.0 bits (52), Expect = 9.3 Identities = 10/27 (37%), Positives = 14/27 (51%) Frame = +2 Query: 530 YWVFTDLSSPSTCTQNKRPEHTPLPSP 610 YW +T +SP+ +N R P P P Sbjct: 497 YWYYTVFNSPTIIEKNFRQSVGPKPIP 523 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,905,204 Number of Sequences: 5004 Number of extensions: 62167 Number of successful extensions: 215 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 203 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 211 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 287744314 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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