Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11k16f
(623 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 93 2e-20
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 87 2e-18
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 66 5e-12
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 52 9e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 46 6e-06
SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 29 0.41
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 28 0.95
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 28 1.3
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 2.9
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 27 2.9
SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa... 26 3.8
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 26 5.1
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo... 26 5.1
SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 25 6.7
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 25 8.9
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 93.5 bits (222), Expect = 2e-20
Identities = 51/134 (38%), Positives = 78/134 (58%), Gaps = 6/134 (4%)
Frame = +1
Query: 223 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 390
+ +Q+AEF+EAF L D D+DG I N+L SLG+ + EL +M+ E +G I+
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 391 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHALMTWGDKFSADEVDE 564
FT+ LT+ A +M +D ++ V AFK FD++G I E L H L + G++ S +EV +
Sbjct: 67 FTEFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVAD 125
Query: 565 AYDQMDIDDKGYID 606
+ D D G I+
Sbjct: 126 MIREADTDGDGVIN 139
Score = 25.4 bits (53), Expect = 6.7
Identities = 17/48 (35%), Positives = 22/48 (45%), Gaps = 1/48 (2%)
Frame = +1
Query: 478 DEEGKID-SERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKL 618
D G ID +E L D + +EV EA+ D D GYI +L
Sbjct: 60 DGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEEL 107
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 87.0 bits (206), Expect = 2e-18
Identities = 47/130 (36%), Positives = 70/130 (53%), Gaps = 2/130 (1%)
Frame = +1
Query: 178 SRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELD 357
++ AKR S F+ + Q+ E KEAF L+D D DG IG+ D++ SL + ASE ++
Sbjct: 28 AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSIN 87
Query: 358 EMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFD--EEGKIDSERLRHALMTW 531
M + PIN LT + + S +D ++ AF TFD + GKI +R AL +
Sbjct: 88 HMFESINPPINLAAFLTAMGSMLCRISPRND-LLEAFSTFDDTQSGKIPISTMRDALSSM 146
Query: 532 GDKFSADEVD 561
GD+ EV+
Sbjct: 147 GDRMDPQEVE 156
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 65.7 bits (153), Expect = 5e-12
Identities = 39/130 (30%), Positives = 69/130 (53%), Gaps = 2/130 (1%)
Frame = +1
Query: 223 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQL 402
S++Q E KEAF L D DKDG+I + + + SLG ++ EL ++ E I+ +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 403 LTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQ 576
++ +N++ E++ I AF+ FD++ G I++ + + T G+K S +EV +
Sbjct: 64 MSFVSNKLRETESEEE-YIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQE 122
Query: 577 MDIDDKGYID 606
D + G D
Sbjct: 123 ADPTNSGSFD 132
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 51.6 bits (118), Expect = 9e-08
Identities = 35/139 (25%), Positives = 65/139 (46%), Gaps = 6/139 (4%)
Frame = +1
Query: 223 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 390
+++Q + EAF+L D DKD I ++LRA +LG A + E+ +++ + G +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 391 FTQLLTLFANRMSGGSDEDDVVINAFKTF--DEEGKIDSERLRHALMTWGDKFSADEVDE 564
+ + ++ D + + AF+ F DE GKI LR + E++
Sbjct: 92 MEDFVRVMTEKIV-ERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEA 150
Query: 565 AYDQMDIDDKGYIDTTKLI 621
++ D+D G I+ + I
Sbjct: 151 MIEEFDLDQDGEINEQEFI 169
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 45.6 bits (103), Expect = 6e-06
Identities = 31/120 (25%), Positives = 56/120 (46%), Gaps = 4/120 (3%)
Frame = +1
Query: 244 FKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANR 423
+K+AF L D G I K + + G+ + E+ E+ ++ Q L + NR
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVDMEQFLQVL-NR 66
Query: 424 MSGGS--DEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDD 591
+G + + + F+ FD++ G I LR+ L + G+K S +E+DE + + D
Sbjct: 67 PNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKD 126
Score = 40.7 bits (91), Expect = 2e-04
Identities = 18/42 (42%), Positives = 26/42 (61%)
Frame = +1
Query: 241 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 366
EF + FQ+ D D G+IG +LR SLG S +E+DE++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119
>SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 452
Score = 29.5 bits (63), Expect = 0.41
Identities = 17/47 (36%), Positives = 26/47 (55%)
Frame = -1
Query: 374 ASPTISSSSFSDASLPRESKVARKSFLPMMPSLSWSISWNASLNSAT 234
AS SSS S +++ V+ S P + + SISWN+S ++AT
Sbjct: 126 ASNYTQSSSNSTSTMNSTGSVSGGSVYPTNSTTNSSISWNSSTSAAT 172
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 28.3 bits (60), Expect = 0.95
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +1
Query: 550 DEVDEAYDQMDIDDKGYID 606
+E +EA+D D+ KGYID
Sbjct: 12 EEAEEAFDLFDVTHKGYID 30
Score = 27.1 bits (57), Expect = 2.2
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = +1
Query: 241 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 366
E +EAF L D G I DLR + LG ++++L M+
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 27.9 bits (59), Expect = 1.3
Identities = 17/46 (36%), Positives = 26/46 (56%)
Frame = -1
Query: 371 SPTISSSSFSDASLPRESKVARKSFLPMMPSLSWSISWNASLNSAT 234
S +++SSS + +SL S + S P + S S+S A+ NSAT
Sbjct: 146 SSSLASSSTTSSSLASSSTNSTTSATPTSSATSSSLSSTAASNSAT 191
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 26.6 bits (56), Expect = 2.9
Identities = 22/83 (26%), Positives = 35/83 (42%)
Frame = -1
Query: 455 TTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASLPRESKVARKSFLPMMPSL 276
+TSSSS L++ S +S ISSSS S +S S S
Sbjct: 242 STSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSSSSSSPTSTSSTISSSSSSSSSPT 301
Query: 275 SWSISWNASLNSATCFCENIEKT 207
S S + ++S +S++ F + +
Sbjct: 302 STSSTISSSSSSSSSFSSTLSSS 324
>SPCC736.14 |dis1||microtubule-associated protein Dis1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 882
Score = 26.6 bits (56), Expect = 2.9
Identities = 24/56 (42%), Positives = 31/56 (55%), Gaps = 2/56 (3%)
Frame = -1
Query: 440 SEPPDMRLANRVK-S*VKL-MGPEASPTISSSSFSDASLPRESKVARKSFLPMMPS 279
S P R+A+ +K S VKL + P+A + SS+ S ASL ES R S P PS
Sbjct: 542 SRPRLPRVASPLKTSPVKLAVTPQAPSPLPSSNPSQASLTEESLSTRSS--PTKPS 595
>SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein
Msa1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 533
Score = 26.2 bits (55), Expect = 3.8
Identities = 23/63 (36%), Positives = 30/63 (47%)
Frame = -1
Query: 464 ALITTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASLPRESKVARKSFLPMM 285
A I S SS+PP R S V+ A ++S+ FS P ES + S LP +
Sbjct: 272 APIPFSVSSDPPSTM--GRSNSAVQSPSYFAHSLVNSTEFST---PNESLSSLPSILPSI 326
Query: 284 PSL 276
PSL
Sbjct: 327 PSL 329
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 25.8 bits (54), Expect = 5.1
Identities = 21/73 (28%), Positives = 35/73 (47%)
Frame = -1
Query: 452 TSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASLPRESKVARKSFLPMMPSLS 273
+SSSS ++ S +S + SSSS S +S S + S +P+ S S
Sbjct: 156 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSSTS 215
Query: 272 WSISWNASLNSAT 234
S S ++S +S++
Sbjct: 216 SSHSSSSSSSSSS 228
>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
Length = 457
Score = 25.8 bits (54), Expect = 5.1
Identities = 11/33 (33%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
Frame = -1
Query: 374 ASPTISSSSFSDASLPRESKVARKSF-LPMMPS 279
+SP++ +++ + P++S V+RKSF P P+
Sbjct: 35 SSPSVPEGTYTVLNNPKQSTVSRKSFSAPTSPT 67
>SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 231
Score = 25.4 bits (53), Expect = 6.7
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = -1
Query: 359 SSSSFSDASLPRESKVARKSFLPMMPSL 276
SSSS S +S P + +RK P MP L
Sbjct: 3 SSSSSSGSSSPASKRSSRKRQTPPMPEL 30
>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1274
Score = 25.0 bits (52), Expect = 8.9
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +1
Query: 232 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASE 345
+ A+FK+ F DKD + + +L A + L +LA +
Sbjct: 212 EYAKFKDKFPEAPLDKDNLQTQKELEARIECLKQLAEK 249
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.315 0.133 0.371
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,759,549
Number of Sequences: 5004
Number of extensions: 28406
Number of successful extensions: 137
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 275671126
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
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