Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11k09f
(603 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g28520.1 68415.m03465 vacuolar proton ATPase, putative simila... 113 9e-26
At2g21410.1 68415.m02548 vacuolar proton ATPase, putative simila... 105 2e-23
At4g39080.1 68417.m05534 vacuolar proton ATPase, putative simila... 104 5e-23
At2g28620.1 68415.m03479 kinesin motor protein-related 31 0.78
At1g08260.1 68414.m00911 DNA-directed DNA polymerase epsilon cat... 30 1.4
At1g13640.1 68414.m01603 phosphatidylinositol 3- and 4-kinase fa... 29 1.8
At4g36470.1 68417.m05179 S-adenosyl-L-methionine:carboxyl methyl... 29 2.4
At4g26965.1 68417.m03880 NADH:ubiquinone oxidoreductase family p... 29 3.1
At1g54095.1 68414.m06165 expressed protein 28 4.1
At5g03420.1 68418.m00295 dentin sialophosphoprotein-related cont... 28 5.5
At1g68790.1 68414.m07863 expressed protein 27 7.2
At1g06750.1 68414.m00717 hypothetical protein 27 7.2
At5g41790.1 68418.m05088 COP1-interactive protein 1 / CIP1 almos... 27 9.6
At4g33390.1 68417.m04746 hypothetical protein contains Pfam prof... 27 9.6
At3g51780.1 68416.m05678 BAG domain-containing protein low simil... 27 9.6
At3g28470.1 68416.m03557 myb family transcription factor (MYB35)... 27 9.6
At3g12020.1 68416.m01490 kinesin motor protein-related similar t... 27 9.6
At2g45000.1 68415.m05603 expressed protein contains Pfam profile... 27 9.6
At2g37420.1 68415.m04589 kinesin motor protein-related 27 9.6
At1g61000.1 68414.m06868 Nuf2 family protein contains Pfam PF038... 27 9.6
>At2g28520.1 68415.m03465 vacuolar proton ATPase, putative similar
to Swiss-Prot:Q93050 vacuolar proton translocating
ATPase 116 kDa subunit A isoform 1 (Clathrin-coated
vesicle/synaptic vesicle proton pump 116 kDa subunit,
Vacuolar proton pump subunit 1, Vacuolar adenosine
triphosphatase subunit Ac116) [Homo sapiens]
Length = 817
Score = 113 bits (272), Expect = 9e-26
Identities = 61/140 (43%), Positives = 86/140 (61%), Gaps = 2/140 (1%)
Frame = +3
Query: 105 MFRSEEMALCQLFIQPEAAYTSVSELGEAGSVQFRDLNPDVNAFQRKFVNEVRRCDEMER 284
+ RSE+M L QL I E+A+ S++ LGE G +QFRDLN D + FQR F N+V+RC EM R
Sbjct: 13 LMRSEKMTLVQLIIPVESAHRSITYLGELGLLQFRDLNADKSPFQRTFANQVKRCGEMSR 72
Query: 285 KLRYIEAEVHKDGVHIPAVKEAPRAPNPREII--DLEAHLEKTENEILELSHNAVNLKQN 458
KLR+ + ++ K G+ +PR +I DLE L E+E+LE++ N+ L+Q
Sbjct: 73 KLRFFKDQIDKAGLRC-----SPRLEIEPDIALGDLERQLADHEHEVLEMNSNSEKLRQT 127
Query: 459 YLELTELRHVLEKTEAFFTA 518
Y EL E + VLEK F +
Sbjct: 128 YNELLEFKIVLEKASGFLVS 147
>At2g21410.1 68415.m02548 vacuolar proton ATPase, putative similar
to vacuolar proton ATPase 100-kDa subunit from
Dictyostelium discoideum P|1384136|gb|AAB49621
Length = 821
Score = 105 bits (252), Expect = 2e-23
Identities = 63/168 (37%), Positives = 98/168 (58%), Gaps = 13/168 (7%)
Frame = +3
Query: 105 MFRSEEMALCQLFIQPEAAYTSVSELGEAGSVQFRDLNPDVNAFQRKFVNEVRRCDEMER 284
+ RSE M L Q+ + E+A+ +VS LG+ G VQF+DLN + + FQR + +++RC EM R
Sbjct: 17 LMRSEPMQLVQVIVPMESAHLTVSYLGDLGLVQFKDLNSEKSPFQRTYAAQIKRCGEMAR 76
Query: 285 KLRYIEAEVHKDGVHIPAVKEAPRAPNPREIIDLEAHLEKTENEILELSHNAVNLKQNYL 464
K+R+ + ++ K GV KE N ++ D+E LE+ E E++E++ N L+++Y
Sbjct: 77 KIRFFKEQMSKAGV---TPKETLDRENDIDLDDVEVKLEELEAELVEINANNDKLQRSYN 133
Query: 465 ELTELRHVLEKTEAFF-------TAQ------EEIGMDSLTKSLISDE 569
EL E + VLEK FF TAQ E++G D L L+ +E
Sbjct: 134 ELVEYKLVLEKAGEFFASAHRSATAQQSEIETEQVGEDLLEAPLLQEE 181
>At4g39080.1 68417.m05534 vacuolar proton ATPase, putative similar
to Swiss-Prot:Q93050 vacuolar proton translocating
ATPase 116 kDa subunit A isoform 1 (Clathrin-coated
vesicle/synaptic vesicle proton pump 116 kDa subunit,
Vacuolar proton pump subunit 1, Vacuolar adenosine
triphosphatase subunit Ac116) [Homo sapiens]
Length = 821
Score = 104 bits (249), Expect = 5e-23
Identities = 56/145 (38%), Positives = 88/145 (60%)
Frame = +3
Query: 105 MFRSEEMALCQLFIQPEAAYTSVSELGEAGSVQFRDLNPDVNAFQRKFVNEVRRCDEMER 284
+ RSE M L QL + E+A+ +VS LG+ G VQF+DLN + + FQR + +++RC EM R
Sbjct: 16 LMRSETMQLVQLIVPMESAHLTVSYLGDLGLVQFKDLNSEKSPFQRTYAAQIKRCGEMAR 75
Query: 285 KLRYIEAEVHKDGVHIPAVKEAPRAPNPREIIDLEAHLEKTENEILELSHNAVNLKQNYL 464
K+R+ ++ K GV PA KE N ++ D+E L + E E++E++ N L+++Y
Sbjct: 76 KIRFFRDQMSKAGV--PA-KEMQGKENDIDLDDVEVKLGELEAELVEINANNDKLQRSYN 132
Query: 465 ELTELRHVLEKTEAFFTAQEEIGMD 539
EL E + VL+K FF++ D
Sbjct: 133 ELMEYKLVLQKAGEFFSSAHRSAAD 157
>At2g28620.1 68415.m03479 kinesin motor protein-related
Length = 1076
Score = 30.7 bits (66), Expect = 0.78
Identities = 30/111 (27%), Positives = 49/111 (44%), Gaps = 4/111 (3%)
Frame = +3
Query: 219 PDVNAFQRKFVNEVRRCDEMERKLRYIEAEVHKDGVHIP----AVKEAPRAPNPREIIDL 386
P+VN K E+ER + + A K+G++IP +EA + +I +
Sbjct: 396 PEVNQKMMKSAIMKDLYSEIERLKQEVYAAREKNGIYIPKERYTQEEAEKKAMADKIEQM 455
Query: 387 EAHLEKTENEILELSHNAVNLKQNYLELTELRHVLEKTEAFFTAQEEIGMD 539
E E + +I++L N +Q L LR L+KTE E+ +D
Sbjct: 456 EVEGEAKDKQIIDL-QELYNSEQ--LVTAGLREKLDKTEKKLYETEQALLD 503
>At1g08260.1 68414.m00911 DNA-directed DNA polymerase epsilon
catalytic subunit, putative similar to SP|Q07864 DNA
polymerase epsilon, catalytic subunit A (EC 2.7.7.7)
(DNA polymerase II subunit A) {Homo sapiens}; contains
Pfam profiles: PF03175 DNA polymerase type B, organellar
and viral, PF00136 DNA polymerase family B, PF03104 DNA
polymerase family B, exonuclease domain
Length = 2271
Score = 29.9 bits (64), Expect = 1.4
Identities = 17/53 (32%), Positives = 32/53 (60%), Gaps = 1/53 (1%)
Frame = +3
Query: 369 REIIDLEAHLEKTENEILELSHNAVNLKQNYLEL-TELRHVLEKTEAFFTAQE 524
+E +DL+ HL + + L++S + V Q +E+ +L H++E+ +A F A E
Sbjct: 122 KEDLDLKNHLSGLQKKYLKISFDTV---QQLMEVKRDLLHIVERNQAKFDALE 171
>At1g13640.1 68414.m01603 phosphatidylinositol 3- and 4-kinase
family protein low similarity to phosphatidylinositol
4-kinase type-II beta [Homo sapiens] GI:20159767;
contains Pfam profile PF00454: Phosphatidylinositol 3-
and 4-kinase
Length = 622
Score = 29.5 bits (63), Expect = 1.8
Identities = 23/70 (32%), Positives = 34/70 (48%), Gaps = 9/70 (12%)
Frame = -2
Query: 275 FIATAHLVNEFPLESIY------VRI*ISELHAASFT--KL*DGGIGRLGLNEELAQSH- 123
F A+ H + FP+ S++ +RI ++ H + KL DGG+GR G E + H
Sbjct: 284 FDASDHGTSSFPVASVHRIGILDIRILNTDRHGGNLLVKKLDDGGVGRFGQVELIPIDHG 343
Query: 122 LLAPEHSPHP 93
L PE P
Sbjct: 344 LCLPETLEDP 353
>At4g36470.1 68417.m05179 S-adenosyl-L-methionine:carboxyl
methyltransferase family protein similar to SAM:jasmonic
acid carboxyl methyltransferase (JMT)[GI:13676829] and
to SAM:salicylic acid carboxyl methyltransferase
(SAMT)[GI:6002712][Clarkia breweri]
Length = 371
Score = 29.1 bits (62), Expect = 2.4
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = -3
Query: 439 ALCDSSRISFSVFSKCASKSMISLG 365
A C + FS+F +C SK M+S G
Sbjct: 197 AYCSQFKEDFSIFLRCRSKEMVSAG 221
>At4g26965.1 68417.m03880 NADH:ubiquinone oxidoreductase family
protein contains Pfam profile PF05071: NADH:ubiquinone
oxidoreductase 17.2 kD subunit
Length = 184
Score = 28.7 bits (61), Expect = 3.1
Identities = 16/62 (25%), Positives = 30/62 (48%), Gaps = 6/62 (9%)
Frame = +3
Query: 243 KFVNEVRRCDEMERKLRYIEAEVHKDGVHIPAV------KEAPRAPNPREIIDLEAHLEK 404
K+ + + D ++ R+++ +D IP + RAP P E+I+LEA E+
Sbjct: 26 KYFSRMEEIDGFVKEKRWVKFRREEDPTSIPVEWICWLNGQRKRAPTPEEMIELEARRER 85
Query: 405 TE 410
+
Sbjct: 86 VK 87
>At1g54095.1 68414.m06165 expressed protein
Length = 150
Score = 28.3 bits (60), Expect = 4.1
Identities = 20/71 (28%), Positives = 29/71 (40%), Gaps = 2/71 (2%)
Frame = -3
Query: 445 LTALCDS--SRISFSVFSKCASKSMISLGFGARGASLTAGMWTPSLCTSASMYRSLRSIS 272
L A+ +S S + F+ C K + G+ L AG + LC+ A Y R
Sbjct: 5 LMAIAESKPSEVGFARCECCGMKEEYTAGYIESVRCLYAGKFICGLCSEAVKYEIFRCGK 64
Query: 271 SQRRTSLTNFL 239
QRR + L
Sbjct: 65 KQRRIGVDEAL 75
>At5g03420.1 68418.m00295 dentin sialophosphoprotein-related
contains weak similarity to Swiss-Prot:Q9NZW4 dentin
sialophosphoprotein precursor [Homo sapiens]
Length = 583
Score = 27.9 bits (59), Expect = 5.5
Identities = 21/69 (30%), Positives = 35/69 (50%), Gaps = 5/69 (7%)
Frame = +3
Query: 315 KDGVHIPAVKEAPRAPNPREI---IDLEAHLE--KTENEILELSHNAVNLKQNYLELTEL 479
K+ +H V + R + R+ +D + H E K EN++ E+ L Q LEL+ L
Sbjct: 406 KEIIHATEVNSSDRNSDQRDGSVGLDTDPHHETRKRENQV-EIDRLRFMLDQKELELSRL 464
Query: 480 RHVLEKTEA 506
+ +EK +A
Sbjct: 465 KEQIEKEKA 473
>At1g68790.1 68414.m07863 expressed protein
Length = 1085
Score = 27.5 bits (58), Expect = 7.2
Identities = 29/115 (25%), Positives = 55/115 (47%), Gaps = 4/115 (3%)
Frame = +3
Query: 237 QRKFVNEVRRCDEMERKLRYIEA--EVH-KDGVHIPAVKEAPRAPNPREIIDLEAHLEKT 407
+ KF R+ E+ERKL+ +E +VH ++ + + +EA A ++ DL+ +K
Sbjct: 191 EEKFSVMNRKSSELERKLKEVETREKVHQREHLSLVTEREAHEAVFYKQREDLQEWEKKL 250
Query: 408 ENEILELSHNAVNLKQNYLELTELRHVLEKTEAFF-TAQEEIGMDSLTKSLISDE 569
E LS ++ + E +EK E Q++I S+ KS ++++
Sbjct: 251 TLEEDRLSEVKRSINHREERVMENERTIEKKEKILENLQQKI---SVAKSELTEK 302
>At1g06750.1 68414.m00717 hypothetical protein
Length = 495
Score = 27.5 bits (58), Expect = 7.2
Identities = 21/59 (35%), Positives = 24/59 (40%)
Frame = +3
Query: 93 RMGAMFRSEEMALCQLFIQPEAAYTSVSELGEAGSVQFRDLNPDVNAFQRKFVNEVRRC 269
+MG R E LC+L A Y S SE E F PD + K V E RC
Sbjct: 44 QMGFKDRRECPNLCKL----AAEYISKSECCEEDIYSFFSEEPDADTLFIKLVEEFERC 98
>At5g41790.1 68418.m05088 COP1-interactive protein 1 / CIP1 almost
identical to CIP1 (GI:836950) [Arabidopsis thaliana]
Length = 1305
Score = 27.1 bits (57), Expect = 9.6
Identities = 20/96 (20%), Positives = 43/96 (44%)
Frame = +3
Query: 168 SVSELGEAGSVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYIEAEVHKDGVHIPAVKE 347
S E ++ S++ +++ + Q + EM+ K + E+E H V + E
Sbjct: 3 SAEEENKSLSLKVSEISDVIQQGQTTIQELISELGEMKEKYKEKESE-HSSLVELHKTHE 61
Query: 348 APRAPNPREIIDLEAHLEKTENEILELSHNAVNLKQ 455
+ +E LEAH+E +E + + + + N ++
Sbjct: 62 RESSSQVKE---LEAHIESSEKLVADFTQSLNNAEE 94
Score = 27.1 bits (57), Expect = 9.6
Identities = 28/135 (20%), Positives = 59/135 (43%)
Frame = +3
Query: 144 IQPEAAYTSVSELGEAGSVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYIEAEVHKDG 323
++ EAAY ++ E + + F++ +N + R +E +++ ++ + G
Sbjct: 1029 VEQEAAYNTLEEEHKQINELFKETEATLNKVTVDYKEAQRLLEERGKEVTSRDSTI---G 1085
Query: 324 VHIPAVKEAPRAPNPREIIDLEAHLEKTENEILELSHNAVNLKQNYLELTELRHVLEKTE 503
VH + E+ R + ++E +EK N ++L + L+ LTE K E
Sbjct: 1086 VHEETM-ESLRNELEMKGDEIETLMEKISNIEVKLRLSNQKLRVTEQVLTEKEEAFRKEE 1144
Query: 504 AFFTAQEEIGMDSLT 548
A ++ + +LT
Sbjct: 1145 AKHLEEQALLEKNLT 1159
>At4g33390.1 68417.m04746 hypothetical protein contains Pfam profile
PF05701: Plant protein of unknown function (DUF827)
Length = 779
Score = 27.1 bits (57), Expect = 9.6
Identities = 33/158 (20%), Positives = 66/158 (41%), Gaps = 12/158 (7%)
Frame = +3
Query: 153 EAAYTS-VSELGEAGSVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYIEAEVHKDGVH 329
+A +TS +SEL E+ + + L + +A ++ V+ +E + +E +V + +
Sbjct: 282 QARHTSAISEL-ESVKEELQTLQNEYDALVKEKDLAVKEAEEAVIASKEVERKVEELTIE 340
Query: 330 IPAVKEAPRAPNPREIIDLEAH-----------LEKTENEILELSHNAVNLKQNYLELTE 476
+ A KE+ + + + E H + E E+ + LKQ+ + E
Sbjct: 341 LIATKESLECAHSSHL-EAEEHRIGAAMLRDQETHRWEKELKQAEEELQRLKQHLVSTKE 399
Query: 477 LRHVLEKTEAFFTAQEEIGMDSLTKSLISDETGQQAAT 590
L+ LE A ++ D S + +ET + T
Sbjct: 400 LQVKLEFASALLLDLKKELADHKESSKVKEETSETVVT 437
>At3g51780.1 68416.m05678 BAG domain-containing protein low
similarity to SP|Q99933 BAG-family molecular chaperone
regulator-1 {Homo sapiens}; contains Pfam profile
PF02179: BAG (Apoptosis regulator Bcl-2 protein) domain
Length = 269
Score = 27.1 bits (57), Expect = 9.6
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = -3
Query: 268 QRRTSLTNFLWKAFTSGFKSLNCTLPASPSS 176
Q + + + W++F +G SLN PASPS+
Sbjct: 229 QSKAAAVSTEWESFGNGVGSLNPPPPASPSA 259
>At3g28470.1 68416.m03557 myb family transcription factor (MYB35)
similar to Atmyb103 GB:AAD40692 from [Arabidopsis
thaliana]; contains PFAM profile: myb DNA binding domain
PF00249
Length = 317
Score = 27.1 bits (57), Expect = 9.6
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = -3
Query: 325 TPSLCTSASMYRSLRSISSQRRTSLTNFLWKAF 227
T S C+S+S S+ + +T +TNF W F
Sbjct: 209 TTSSCSSSSSSTSITQPNQVPQTPVTNFYWSDF 241
>At3g12020.1 68416.m01490 kinesin motor protein-related similar to
putative kinesin heavy chain GB:AAD23684 GI:4567271 from
[Arabidopsis thaliana]
Length = 1030
Score = 27.1 bits (57), Expect = 9.6
Identities = 19/77 (24%), Positives = 37/77 (48%)
Frame = +3
Query: 372 EIIDLEAHLEKTENEILELSHNAVNLKQNYLELTELRHVLEKTEAFFTAQEEIGMDSLTK 551
+IID ++ ++K + EI +L LKQ + + +L+ + + E G L +
Sbjct: 396 KIIDEKSLIKKYQREIRQLKEELEQLKQEIVPVPQLKDIGADDIVLLKQKLEDGQVKL-Q 454
Query: 552 SLISDETGQQAATRGRL 602
S + +E +AA R+
Sbjct: 455 SRLEEEEEAKAALLSRI 471
>At2g45000.1 68415.m05603 expressed protein contains Pfam profile:
PF05064 Nsp1-like C-terminal region
Length = 739
Score = 27.1 bits (57), Expect = 9.6
Identities = 17/48 (35%), Positives = 26/48 (54%)
Frame = -3
Query: 442 TALCDSSRISFSVFSKCASKSMISLGFGARGASLTAGMWTPSLCTSAS 299
+A +S +SFS F++ ++ S GFG+ +S A TPS AS
Sbjct: 27 SASSTTSPLSFS-FNQSSNPSSTGFGFGSSVSSTPASSTTPSFGFGAS 73
>At2g37420.1 68415.m04589 kinesin motor protein-related
Length = 1039
Score = 27.1 bits (57), Expect = 9.6
Identities = 16/71 (22%), Positives = 38/71 (53%)
Frame = +3
Query: 258 VRRCDEMERKLRYIEAEVHKDGVHIPAVKEAPRAPNPREIIDLEAHLEKTENEILELSHN 437
+ R +++E +L E+EV K KE +++D+E+ L+ + + + +
Sbjct: 447 IERIEQLENELNLSESEVSKFCDLYETEKE--------KLLDVESDLKDCKRNLHNSNKD 498
Query: 438 AVNLKQNYLEL 470
++LK+NY+++
Sbjct: 499 LLDLKENYIQV 509
>At1g61000.1 68414.m06868 Nuf2 family protein contains Pfam PF03800:
Nuf2 family domain; similar to Myosin-like protein NUF2
(Nuclear filament-containing protein 2) (Nuclear
division protein nuf2) (Swiss-Prot:Q10173)
[Schizosaccharomyces pombe]
Length = 974
Score = 27.1 bits (57), Expect = 9.6
Identities = 10/32 (31%), Positives = 23/32 (71%)
Frame = +3
Query: 381 DLEAHLEKTENEILELSHNAVNLKQNYLELTE 476
+LEA++E+ +ILEL++ ++L+ + ++ E
Sbjct: 183 ELEANIEQLNKKILELNNQQMSLRATFQKMRE 214
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,169,184
Number of Sequences: 28952
Number of extensions: 317969
Number of successful extensions: 948
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 910
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 946
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1197101088
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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