BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11j23f (584 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC2F12.05c |||sterol binding ankyrin repeat protein|Schizosacc... 66 3e-12 SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe... 30 0.29 SPAC22E12.18 |||conserved fungal protein|Schizosaccharomyces pom... 29 0.66 SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit ... 26 3.5 SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 26 4.7 SPBC11B10.02c |his3||histidinol-phosphate aminotransferase imida... 26 4.7 SPBC582.07c |rpn7||19S proteasome regulatory subunit Rpn7|Schizo... 25 6.2 SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 25 8.1 SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 25 8.1 >SPBC2F12.05c |||sterol binding ankyrin repeat protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 1310 Score = 66.5 bits (155), Expect = 3e-12 Identities = 33/90 (36%), Positives = 53/90 (58%), Gaps = 1/90 (1%) Frame = +2 Query: 149 LRGYLSKWTNYIHGWQDRFIVLKDSTLSYYKNELESNLGCRGALCLKKAKVKPHEFDDCR 328 + GYL KWTNY G++ R+ L + LSYYKN+ +++ CRG++ LK A++ Sbjct: 257 MSGYLKKWTNYKSGYKLRWFTLNNGVLSYYKNQDDASSACRGSINLKLARLVHDPKQPTV 316 Query: 329 FDVSVSDCVWY-LRASNPEEKQQWIDILES 415 F V V Y ++A++P E ++WI + S Sbjct: 317 FQVIGKGSVRYSVKANSPVEAKKWIAAISS 346 >SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 871 Score = 29.9 bits (64), Expect = 0.29 Identities = 12/33 (36%), Positives = 20/33 (60%) Frame = +2 Query: 140 TPELRGYLSKWTNYIHGWQDRFIVLKDSTLSYY 238 T + G++ K ++ W+ R+ VLK + LSYY Sbjct: 698 TADCHGWMRKRSDRYGVWKSRYFVLKGTRLSYY 730 >SPAC22E12.18 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 336 Score = 28.7 bits (61), Expect = 0.66 Identities = 17/33 (51%), Positives = 17/33 (51%) Frame = -3 Query: 492 AEPCL*RPPPFELLSELVPYPDSTLKDSRISIH 394 A CL P EL SELV P S LKD IH Sbjct: 29 ARKCLDDMKPIELPSELVESPLSMLKDVATLIH 61 >SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit Bgs4|Schizosaccharomyces pombe|chr 3|||Manual Length = 1955 Score = 26.2 bits (55), Expect = 3.5 Identities = 13/43 (30%), Positives = 19/43 (44%), Gaps = 1/43 (2%) Frame = +2 Query: 275 ALCLKKAKVKPHEFDDCRFDVSVSDCV-WYLRASNPEEKQQWI 400 ALC+ PH+FD F V + + W R ++ WI Sbjct: 1543 ALCVAPFLFNPHQFDWNDFFVDYREFIRWLSRGNSRSHANSWI 1585 >SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 25.8 bits (54), Expect = 4.7 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = +2 Query: 500 LQSTGSHGHSTRRLTEKITELETYNEL 580 L+ H +LTE+I +LET N+L Sbjct: 411 LEENALLSHKVLKLTEEIQDLETLNQL 437 >SPBC11B10.02c |his3||histidinol-phosphate aminotransferase imidazole acetol phosphate transaminase His3|Schizosaccharomyces pombe|chr 2|||Manual Length = 384 Score = 25.8 bits (54), Expect = 4.7 Identities = 10/21 (47%), Positives = 16/21 (76%) Frame = -2 Query: 580 KLIVRFQLSDFLCEATCRVTV 518 K++VRF+ ++ LCE R+TV Sbjct: 340 KVVVRFRGTEPLCEGALRITV 360 >SPBC582.07c |rpn7||19S proteasome regulatory subunit Rpn7|Schizosaccharomyces pombe|chr 2|||Manual Length = 409 Score = 25.4 bits (53), Expect = 6.2 Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = -3 Query: 117 LSLTVMFSSIILCYVYGSVN-IKSTLNKMTPNNEK 16 + + V+FS I L YVY + + L K+ P EK Sbjct: 147 MKIDVLFSKIRLAYVYADMRVVGQLLEKLKPLIEK 181 >SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1647 Score = 25.0 bits (52), Expect = 8.1 Identities = 11/46 (23%), Positives = 19/46 (41%) Frame = -3 Query: 159 YPRSSGVPPSSKSELSLTVMFSSIILCYVYGSVNIKSTLNKMTPNN 22 Y G+PPS S + + S+ C + N+ + + P N Sbjct: 641 YEMLCGIPPSDTSHQADMITMQSLYYCPIELIENLLRAITSLLPKN 686 >SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizosaccharomyces pombe|chr 3|||Manual Length = 640 Score = 25.0 bits (52), Expect = 8.1 Identities = 12/39 (30%), Positives = 18/39 (46%) Frame = -3 Query: 513 PVDCSEVAEPCL*RPPPFELLSELVPYPDSTLKDSRISI 397 PVD + P + RPP + LS V D ++ S + Sbjct: 317 PVDTHTPSTPIMSRPPSMKALSSGVESQDESVASSNFQV 355 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,171,417 Number of Sequences: 5004 Number of extensions: 39172 Number of successful extensions: 132 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 130 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 132 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 252150250 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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