Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11j23f
(584 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 44 1e-04
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 44 1e-04
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 44 1e-04
At2g29700.1 68415.m03610 pleckstrin homology (PH) domain-contain... 44 1e-04
At4g12460.1 68417.m01971 oxysterol-binding family protein simila... 43 1e-04
At2g31020.1 68415.m03782 oxysterol-binding family protein simila... 43 1e-04
At5g05710.1 68418.m00628 pleckstrin homology (PH) domain-contain... 42 2e-04
At1g77730.1 68414.m09050 pleckstrin homology (PH) domain-contain... 42 4e-04
At1g13170.1 68414.m01527 oxysterol-binding family protein simila... 40 0.002
At4g22540.1 68417.m03253 oxysterol-binding family protein simila... 38 0.004
At1g10290.1 68414.m01159 dynamin-like protein 6 (ADL6) identical... 33 0.11
At1g59610.1 68414.m06704 dynamin-like protein, putative (ADL3) s... 32 0.24
At5g25190.1 68418.m02986 ethylene-responsive element-binding pro... 30 1.3
At3g60050.1 68416.m06706 pentatricopeptide (PPR) repeat-containi... 28 5.3
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 43.6 bits (98), Expect = 1e-04
Identities = 17/31 (54%), Positives = 21/31 (67%)
Frame = +2
Query: 149 LRGYLSKWTNYIHGWQDRFIVLKDSTLSYYK 241
+ G L KW NY GW+ R+ VL+D LSYYK
Sbjct: 106 IAGILYKWVNYGRGWRPRWFVLQDGVLSYYK 136
Score = 32.7 bits (71), Expect = 0.19
Identities = 15/50 (30%), Positives = 26/50 (52%)
Frame = +2
Query: 272 GALCLKKAKVKPHEFDDCRFDVSVSDCVWYLRASNPEEKQQWIDILESFK 421
G + LK + V+ DD RF + +LRA E++ W++ L++ K
Sbjct: 185 GEVHLKVSSVRESRSDDKRFSIFTGTKRLHLRAETREDRTTWVEALQAVK 234
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 43.6 bits (98), Expect = 1e-04
Identities = 17/31 (54%), Positives = 21/31 (67%)
Frame = +2
Query: 149 LRGYLSKWTNYIHGWQDRFIVLKDSTLSYYK 241
+ G L KW NY GW+ R+ VL+D LSYYK
Sbjct: 106 IAGILYKWVNYGRGWRPRWFVLQDGVLSYYK 136
Score = 32.7 bits (71), Expect = 0.19
Identities = 15/50 (30%), Positives = 26/50 (52%)
Frame = +2
Query: 272 GALCLKKAKVKPHEFDDCRFDVSVSDCVWYLRASNPEEKQQWIDILESFK 421
G + LK + V+ DD RF + +LRA E++ W++ L++ K
Sbjct: 185 GEVHLKVSSVRESRSDDKRFSIFTGTKRLHLRAETREDRTTWVEALQAVK 234
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 43.6 bits (98), Expect = 1e-04
Identities = 17/31 (54%), Positives = 21/31 (67%)
Frame = +2
Query: 149 LRGYLSKWTNYIHGWQDRFIVLKDSTLSYYK 241
+ G L KW NY GW+ R+ VL+D LSYYK
Sbjct: 106 IAGILYKWVNYGRGWRPRWFVLQDGVLSYYK 136
Score = 32.7 bits (71), Expect = 0.19
Identities = 15/50 (30%), Positives = 26/50 (52%)
Frame = +2
Query: 272 GALCLKKAKVKPHEFDDCRFDVSVSDCVWYLRASNPEEKQQWIDILESFK 421
G + LK + V+ DD RF + +LRA E++ W++ L++ K
Sbjct: 185 GEVHLKVSSVRESRSDDKRFSIFTGTKRLHLRAETREDRTTWVEALQAVK 234
>At2g29700.1 68415.m03610 pleckstrin homology (PH) domain-containing
protein (PH1) identical to AtPH1 [Arabidopsis thaliana]
GI:5926716; contains Pfam profile PF00169: PH domain
Length = 145
Score = 43.6 bits (98), Expect = 1e-04
Identities = 29/104 (27%), Positives = 50/104 (48%), Gaps = 7/104 (6%)
Frame = +2
Query: 113 DSSDLEEGGTPELRGYLSKWTNYIHGWQDRFIVLKDSTLSYYKNELESNL-GC--RGAL- 280
D +E PE G+L+K +YI W+ R+ VLK L ++K++ + + G RG +
Sbjct: 17 DYEGIEFWSNPERSGWLTKQGDYIKTWRRRWFVLKRGKLLWFKDQAAAGIRGSTPRGVIS 76
Query: 281 ---CLKKAKVKPHEFDDCRFDVSVSDCVWYLRASNPEEKQQWID 403
CL + F++S + A N +EK++WI+
Sbjct: 77 VGDCLTVKGAEDVVNKPFAFELSSGSYTMFFIADNEKEKEEWIN 120
>At4g12460.1 68417.m01971 oxysterol-binding family protein similar
to SP|P22059 Oxysterol-binding protein 1 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 694
Score = 43.2 bits (97), Expect = 1e-04
Identities = 21/51 (41%), Positives = 29/51 (56%)
Frame = +2
Query: 110 SDSSDLEEGGTPELRGYLSKWTNYIHGWQDRFIVLKDSTLSYYKNELESNL 262
SD L+ G + + G L KWTNY GW+ R+ +L+D LSY K N+
Sbjct: 17 SDRETLQSGRS--VAGILYKWTNYGKGWRSRWFLLRDGILSYSKIRRPENV 65
>At2g31020.1 68415.m03782 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 760
Score = 43.2 bits (97), Expect = 1e-04
Identities = 17/31 (54%), Positives = 21/31 (67%)
Frame = +2
Query: 149 LRGYLSKWTNYIHGWQDRFIVLKDSTLSYYK 241
+ G L KW NY GW+ R+ VL+D LSYYK
Sbjct: 73 IAGILYKWVNYGKGWRPRWFVLQDGVLSYYK 103
Score = 33.9 bits (74), Expect = 0.080
Identities = 15/50 (30%), Positives = 27/50 (54%)
Frame = +2
Query: 272 GALCLKKAKVKPHEFDDCRFDVSVSDCVWYLRASNPEEKQQWIDILESFK 421
G + LK + ++ DD RF + +LRA E+++ WI+ L++ K
Sbjct: 153 GEVHLKVSSIRESRSDDKRFSIFTGTKRLHLRAETREDREAWIEALQAVK 202
>At5g05710.1 68418.m00628 pleckstrin homology (PH) domain-containing
protein similar to AtPH1 [Arabidopsis thaliana]
GI:5926716; contains Pfam profile PF00169: PH domain
Length = 144
Score = 42.3 bits (95), Expect = 2e-04
Identities = 29/108 (26%), Positives = 49/108 (45%), Gaps = 5/108 (4%)
Frame = +2
Query: 95 ENITVSDSSDLEEGGTPELRGYLSKWTNYIHGWQDRFIVLKDSTLSYYKNELESNLG-CR 271
+N D +E PE G+L+K YI W+ R+ VLK L ++K+ + + R
Sbjct: 12 QNNNSEDYGGVEFWSNPERTGWLTKQGEYIKTWRRRWFVLKQGKLFWFKDSDVTRVSRPR 71
Query: 272 GAL----CLKKAKVKPHEFDDCRFDVSVSDCVWYLRASNPEEKQQWID 403
G + CL + F++S + Y A + +EK+ WI+
Sbjct: 72 GVVPVESCLTAKGAEDVLNKQNAFELSTRNETMYFIADSEKEKEDWIN 119
>At1g77730.1 68414.m09050 pleckstrin homology (PH) domain-containing
protein similar to SP|P16258 Oxysterol-binding protein 1
{Oryctolagus cuniculus}; contains Pfam profile PF00169:
PH domain
Length = 265
Score = 41.5 bits (93), Expect = 4e-04
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +2
Query: 155 GYLSKWTNYIHGWQDRFIVLKDSTLSYYK 241
G L KW NY GW+ R+ VL+D LSYY+
Sbjct: 56 GILYKWVNYGQGWKRRWFVLQDGVLSYYR 84
Score = 28.7 bits (61), Expect = 3.0
Identities = 15/50 (30%), Positives = 25/50 (50%)
Frame = +2
Query: 272 GALCLKKAKVKPHEFDDCRFDVSVSDCVWYLRASNPEEKQQWIDILESFK 421
G + LK + + D RF V +LRA+ E++ WI+ L++ K
Sbjct: 129 GQIHLKVSSIGQSISDGKRFTVFTGTKSLHLRAATSEDRASWIEALKAVK 178
>At1g13170.1 68414.m01527 oxysterol-binding family protein similar
to SP|P16258 Oxysterol-binding protein 1 {Oryctolagus
cuniculus}; contains Pfam profiles PF00169: PH domain,
PF01237: Oxysterol-binding protein
Length = 816
Score = 39.5 bits (88), Expect = 0.002
Identities = 16/36 (44%), Positives = 21/36 (58%)
Frame = +2
Query: 134 GGTPELRGYLSKWTNYIHGWQDRFIVLKDSTLSYYK 241
G + G + KW NY GW+ R+ L+D LSYYK
Sbjct: 90 GAGAGVAGIMYKWVNYGKGWRARWFELEDGVLSYYK 125
>At4g22540.1 68417.m03253 oxysterol-binding family protein similar
to SP|P16258 Oxysterol-binding protein 1 {Oryctolagus
cuniculus}; contains Pfam profiles PF00169: PH domain,
PF01237: Oxysterol-binding protein
Length = 721
Score = 38.3 bits (85), Expect = 0.004
Identities = 16/36 (44%), Positives = 22/36 (61%)
Frame = +2
Query: 155 GYLSKWTNYIHGWQDRFIVLKDSTLSYYKNELESNL 262
G L KWTN+ GW+ R+ +L++ LSY K NL
Sbjct: 55 GILYKWTNFGKGWRSRWFLLRNGILSYSKIRRPENL 90
>At1g10290.1 68414.m01159 dynamin-like protein 6 (ADL6) identical to
dynamin-like protein 6 (ADL6) [Arabidopsis thaliana]
GI:6651399; contains Pfam profiles PF01031: Dynamin
central region, PF00350: Dynamin family, PF02212:
Dynamin GTPase effector domain, PF00169: PH domain
Length = 914
Score = 33.5 bits (73), Expect = 0.11
Identities = 24/80 (30%), Positives = 38/80 (47%), Gaps = 2/80 (2%)
Frame = +2
Query: 155 GYLSKWTNYIHGWQDRFIVLKDST--LSYYKNELESNLGCRGALCLKKAKVKPHEFDDCR 328
GYL K + +GW R+ VL + T L Y K + E N RG + L++ ++ D+
Sbjct: 577 GYLMKKSAKTNGWSRRWFVLNEKTGKLGYTKKQEERNF--RGTITLEECTIEEIPEDEVE 634
Query: 329 FDVSVSDCVWYLRASNPEEK 388
S D +A+ P+ K
Sbjct: 635 KSKSSKD----KKANGPDSK 650
>At1g59610.1 68414.m06704 dynamin-like protein, putative (ADL3)
strong similarity to dynamin-like protein 6 (ADL6)
[Arabidopsis thaliana] GI:6651399; contains Pfam
profiles PF01031: Dynamin central region, PF00350:
Dynamin family, PF02212: Dynamin GTPase effector domain,
PF00169: PH domain; identical to cDNA dynamin-like
protein ADL3, GI:4803835
Length = 920
Score = 32.3 bits (70), Expect = 0.24
Identities = 19/58 (32%), Positives = 30/58 (51%), Gaps = 2/58 (3%)
Frame = +2
Query: 155 GYLSKWTNYIHGWQDRFIVLKDST--LSYYKNELESNLGCRGALCLKKAKVKPHEFDD 322
GYL K + +GW R+ VL + T L Y K + E N RG + L++ ++ D+
Sbjct: 584 GYLMKKSAKTNGWSRRWFVLNEKTGKLGYTKKQEERNF--RGTVTLEECSIEEISDDE 639
>At5g25190.1 68418.m02986 ethylene-responsive element-binding
protein, putative ethylene responsive element binding
protein homolog, Stylosanthes hamata, EMBL:U91857
Length = 181
Score = 29.9 bits (64), Expect = 1.3
Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 3/51 (5%)
Frame = -3
Query: 441 VPYPDSTLKDSRIS--IHCCFSSGLDALKY-QTQSLTETSNLQSSNS*GLT 298
+P S L + ++ +H C+ + L K QTQ+ T+T+ QS++S G+T
Sbjct: 70 IPTSSSKLLSATLTAKLHKCYMASLQMTKQTQTQTQTQTARSQSADSDGVT 120
>At3g60050.1 68416.m06706 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 473
Score = 27.9 bits (59), Expect = 5.3
Identities = 15/33 (45%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = +2
Query: 125 LEEGGTPELRGY-LSKWTNYIHGWQDRFIVLKD 220
LE+G +P++ Y + WTNY G DRF L D
Sbjct: 246 LEDGFSPDVLTYNILLWTNYRLGKMDRFDRLFD 278
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,100,112
Number of Sequences: 28952
Number of extensions: 197796
Number of successful extensions: 544
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 533
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 542
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1151426952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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