BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11j15r (626 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g15230.1 68415.m01737 lipase family protein similar to SP|P07... 82 3e-16 At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64... 58 5e-09 At3g23280.2 68416.m02935 zinc finger (C3HC4-type RING finger) fa... 28 4.4 At3g23280.1 68416.m02934 zinc finger (C3HC4-type RING finger) fa... 28 4.4 At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar... 28 4.4 At3g57570.1 68416.m06410 expressed protein 28 5.8 At3g16090.1 68416.m02033 zinc finger (C3HC4-type RING finger) fa... 27 7.7 >At2g15230.1 68415.m01737 lipase family protein similar to SP|P07098 Triacylglycerol lipase, gastric precursor (EC 3.1.1.3) {Homo sapiens}; contains Pfam profile PF04083: ab-hydrolase associated lipase region Length = 393 Score = 81.8 bits (193), Expect = 3e-16 Identities = 61/204 (29%), Positives = 99/204 (48%), Gaps = 3/204 (1%) Frame = -2 Query: 625 PVAYLEFNANRLL-KLIAPHANSIEALTSLIGINELFGRSDFFTNVGMRFCADGVFFQAM 449 P++YL+ L+ +++ H ++ + +G++++ RSD + C +G Sbjct: 193 PISYLDHVTAPLVERMVFMH---LDQMVVALGLHQINFRSDMLVKLVDSLC-EG---HMD 245 Query: 448 CTNMLFAFAGRSEDMHNATMLPVKLGHTPAGIAVRQIAHYGQLINNNFFRRYDHGRLKNW 269 CT+ L + G + NA+ + L + P +V+ I H Q+I F +YD+G KN Sbjct: 246 CTDFLTSITG-TNCCFNASKIEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNL 304 Query: 268 RVYRSFTPPRYDLSLITA--PVFLHYVDDDIFADVRDVRKLQIELGRPVGMFRVPHATFS 95 R Y PP + LS I A P+++ Y D ADV DV EL P + + Sbjct: 305 RTYGLSKPPEFILSHIPASLPMWMGYGGTDGLADVTDVEHTLAEL--PSSPELLYLEDYG 362 Query: 94 HLDFMWGSGAKELLYDRTIQIMRS 23 H+DF+ GS AKE +Y IQ R+ Sbjct: 363 HIDFVLGSSAKEDVYKHMIQFFRA 386 >At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64194 Lysosomal acid lipase/cholesteryl ester hydrolase precursor (EC 3.1.1.13) {Rattus norvegicus}; contains Pfam profile PF04083: ab-hydrolase associated lipase region Length = 418 Score = 58.0 bits (134), Expect = 5e-09 Identities = 51/199 (25%), Positives = 92/199 (46%), Gaps = 5/199 (2%) Frame = -2 Query: 625 PVAYLEFNANRLLKLIAPHANSIEALTSLIGINELFGRSDFFTNVGMRFCADGVFFQAMC 446 PVAYL + ++ IA EA TS++G E +S + C C Sbjct: 217 PVAYLS-HMTTVIGDIAAKTFLAEA-TSILGWPEFNPKSGLVGDFIKAICLKAGID---C 271 Query: 445 TNMLFAFAGRSEDMHNATMLPVKLGHTPAGIAVRQIAHYGQLINNNFFRRYDHGRL-KNW 269 +++ G++ + NA+ + + L + P + + + H Q + + R+Y++G +N Sbjct: 272 YDLVSVITGKNCCL-NASTIDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNI 330 Query: 268 RVYRSFTPPRYDLSLIT--APVFLHYVDDDIFADVRDVRKLQIELG-RPVGMFRVPHAT- 101 + Y PP Y++S I P+F Y D ADV+DV L + + V Sbjct: 331 KHYGQAIPPAYNISAIPHELPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKMNVQFVKD 390 Query: 100 FSHLDFMWGSGAKELLYDR 44 ++H DF+ G AK+++Y++ Sbjct: 391 YAHADFIMGVTAKDVVYNQ 409 >At3g23280.2 68416.m02935 zinc finger (C3HC4-type RING finger) family protein / ankyrin repeat family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) and Pfam profile: PF00023 ankyrin repeat Length = 438 Score = 28.3 bits (60), Expect = 4.4 Identities = 17/35 (48%), Positives = 20/35 (57%) Frame = +3 Query: 333 CAICLTAMPAGVCPSFTGNIVALCMSSLRPAKANN 437 CAICL A VC G+ VA CMS L+ K+ N Sbjct: 387 CAICLDAPSEAVCVP-CGH-VAGCMSCLKEIKSKN 419 >At3g23280.1 68416.m02934 zinc finger (C3HC4-type RING finger) family protein / ankyrin repeat family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) and Pfam profile: PF00023 ankyrin repeat Length = 462 Score = 28.3 bits (60), Expect = 4.4 Identities = 17/35 (48%), Positives = 20/35 (57%) Frame = +3 Query: 333 CAICLTAMPAGVCPSFTGNIVALCMSSLRPAKANN 437 CAICL A VC G+ VA CMS L+ K+ N Sbjct: 411 CAICLDAPSEAVCVP-CGH-VAGCMSCLKEIKSKN 443 >At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar to RNA helicase [Rattus norvegicus] GI:897915; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 1166 Score = 28.3 bits (60), Expect = 4.4 Identities = 11/25 (44%), Positives = 16/25 (64%) Frame = +1 Query: 415 CVQQKRITYLCTSPGKIRHQRKISF 489 C + I LCTS GKI + R+++F Sbjct: 658 CTPGRMIDILCTSSGKITNLRRVTF 682 >At3g57570.1 68416.m06410 expressed protein Length = 1057 Score = 27.9 bits (59), Expect = 5.8 Identities = 14/30 (46%), Positives = 20/30 (66%) Frame = -2 Query: 622 VAYLEFNANRLLKLIAPHANSIEALTSLIG 533 V L F+++R+LKLI A S++ SLIG Sbjct: 587 VTGLTFDSDRVLKLIPEWARSVQNWNSLIG 616 >At3g16090.1 68416.m02033 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) Length = 492 Score = 27.5 bits (58), Expect = 7.7 Identities = 13/29 (44%), Positives = 19/29 (65%), Gaps = 2/29 (6%) Frame = +1 Query: 409 LHCVQQKRITYLCTSP--GKIRHQRKISF 489 LH + QKR+ Y+ T+P K+ H R +SF Sbjct: 116 LHWLAQKRVEYIETTPSVSKLSHFRIVSF 144 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,749,155 Number of Sequences: 28952 Number of extensions: 292961 Number of successful extensions: 675 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 662 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 673 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1275599520 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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