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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV11j15f
         (615 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g15230.1 68415.m01737 lipase family protein similar to SP|P07...    82   3e-16
At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64...    58   5e-09
At3g23280.2 68416.m02935 zinc finger (C3HC4-type RING finger) fa...    28   4.3  
At3g23280.1 68416.m02934 zinc finger (C3HC4-type RING finger) fa...    28   4.3  
At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar...    28   4.3  
At3g57570.1 68416.m06410 expressed protein                             28   5.7  
At3g16090.1 68416.m02033 zinc finger (C3HC4-type RING finger) fa...    27   7.5  
At3g09620.1 68416.m01141 DEAD/DEAH box helicase, putative simila...    27   9.9  

>At2g15230.1 68415.m01737 lipase family protein similar to SP|P07098
           Triacylglycerol lipase, gastric precursor (EC 3.1.1.3)
           {Homo sapiens}; contains Pfam profile PF04083:
           ab-hydrolase associated lipase region
          Length = 393

 Score = 81.8 bits (193), Expect = 3e-16
 Identities = 61/204 (29%), Positives = 99/204 (48%), Gaps = 3/204 (1%)
 Frame = +2

Query: 2   PVAYLEFNANRLL-KLIAPHANSIEALTSLIGINELFGRSDFFTNVGMRFCADGVFFQAM 178
           P++YL+     L+ +++  H   ++ +   +G++++  RSD    +    C +G      
Sbjct: 193 PISYLDHVTAPLVERMVFMH---LDQMVVALGLHQINFRSDMLVKLVDSLC-EG---HMD 245

Query: 179 CTNMLFAFAGRSEDMHNATMLPVKLGHTPAGIAVRQIAHYGQLINNNFFRRYDHGRLKNW 358
           CT+ L +  G +    NA+ +   L + P   +V+ I H  Q+I    F +YD+G  KN 
Sbjct: 246 CTDFLTSITG-TNCCFNASKIEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNL 304

Query: 359 RVYRSFTPPRYDLSLITA--PVFLHYVDDDIFADVRDVRKLQIELGRPVGMFRVPHATFS 532
           R Y    PP + LS I A  P+++ Y   D  ADV DV     EL  P     +    + 
Sbjct: 305 RTYGLSKPPEFILSHIPASLPMWMGYGGTDGLADVTDVEHTLAEL--PSSPELLYLEDYG 362

Query: 533 HLDFMWGSGAKELLYDRTIQIMRS 604
           H+DF+ GS AKE +Y   IQ  R+
Sbjct: 363 HIDFVLGSSAKEDVYKHMIQFFRA 386


>At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64194
           Lysosomal acid lipase/cholesteryl ester hydrolase
           precursor (EC 3.1.1.13) {Rattus norvegicus}; contains
           Pfam profile PF04083: ab-hydrolase associated lipase
           region
          Length = 418

 Score = 58.0 bits (134), Expect = 5e-09
 Identities = 51/199 (25%), Positives = 92/199 (46%), Gaps = 5/199 (2%)
 Frame = +2

Query: 2   PVAYLEFNANRLLKLIAPHANSIEALTSLIGINELFGRSDFFTNVGMRFCADGVFFQAMC 181
           PVAYL  +   ++  IA      EA TS++G  E   +S    +     C         C
Sbjct: 217 PVAYLS-HMTTVIGDIAAKTFLAEA-TSILGWPEFNPKSGLVGDFIKAICLKAGID---C 271

Query: 182 TNMLFAFAGRSEDMHNATMLPVKLGHTPAGIAVRQIAHYGQLINNNFFRRYDHGRL-KNW 358
            +++    G++  + NA+ + + L + P   + + + H  Q + +   R+Y++G   +N 
Sbjct: 272 YDLVSVITGKNCCL-NASTIDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNI 330

Query: 359 RVYRSFTPPRYDLSLIT--APVFLHYVDDDIFADVRDVRKLQIELG-RPVGMFRVPHAT- 526
           + Y    PP Y++S I    P+F  Y   D  ADV+DV  L  +     +    V     
Sbjct: 331 KHYGQAIPPAYNISAIPHELPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKMNVQFVKD 390

Query: 527 FSHLDFMWGSGAKELLYDR 583
           ++H DF+ G  AK+++Y++
Sbjct: 391 YAHADFIMGVTAKDVVYNQ 409


>At3g23280.2 68416.m02935 zinc finger (C3HC4-type RING finger)
           family protein / ankyrin repeat family protein contains
           Pfam profile: PF00097 zinc finger, C3HC4 type (RING
           finger) and Pfam profile: PF00023 ankyrin repeat
          Length = 438

 Score = 28.3 bits (60), Expect = 4.3
 Identities = 17/35 (48%), Positives = 20/35 (57%)
 Frame = -1

Query: 294 CAICLTAMPAGVCPSFTGNIVALCMSSLRPAKANN 190
           CAICL A    VC    G+ VA CMS L+  K+ N
Sbjct: 387 CAICLDAPSEAVCVP-CGH-VAGCMSCLKEIKSKN 419


>At3g23280.1 68416.m02934 zinc finger (C3HC4-type RING finger)
           family protein / ankyrin repeat family protein contains
           Pfam profile: PF00097 zinc finger, C3HC4 type (RING
           finger) and Pfam profile: PF00023 ankyrin repeat
          Length = 462

 Score = 28.3 bits (60), Expect = 4.3
 Identities = 17/35 (48%), Positives = 20/35 (57%)
 Frame = -1

Query: 294 CAICLTAMPAGVCPSFTGNIVALCMSSLRPAKANN 190
           CAICL A    VC    G+ VA CMS L+  K+ N
Sbjct: 411 CAICLDAPSEAVCVP-CGH-VAGCMSCLKEIKSKN 443


>At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar to
           RNA helicase [Rattus norvegicus] GI:897915; contains
           Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
           Helicase conserved C-terminal domain
          Length = 1166

 Score = 28.3 bits (60), Expect = 4.3
 Identities = 11/25 (44%), Positives = 16/25 (64%)
 Frame = -2

Query: 212 CVQQKRITYLCTSPGKIRHQRKISF 138
           C   + I  LCTS GKI + R+++F
Sbjct: 658 CTPGRMIDILCTSSGKITNLRRVTF 682


>At3g57570.1 68416.m06410 expressed protein 
          Length = 1057

 Score = 27.9 bits (59), Expect = 5.7
 Identities = 14/30 (46%), Positives = 20/30 (66%)
 Frame = +2

Query: 5   VAYLEFNANRLLKLIAPHANSIEALTSLIG 94
           V  L F+++R+LKLI   A S++   SLIG
Sbjct: 587 VTGLTFDSDRVLKLIPEWARSVQNWNSLIG 616


>At3g16090.1 68416.m02033 zinc finger (C3HC4-type RING finger)
           family protein contains Pfam profile: PF00097 zinc
           finger, C3HC4 type (RING finger)
          Length = 492

 Score = 27.5 bits (58), Expect = 7.5
 Identities = 13/29 (44%), Positives = 19/29 (65%), Gaps = 2/29 (6%)
 Frame = -2

Query: 218 LHCVQQKRITYLCTSP--GKIRHQRKISF 138
           LH + QKR+ Y+ T+P   K+ H R +SF
Sbjct: 116 LHWLAQKRVEYIETTPSVSKLSHFRIVSF 144


>At3g09620.1 68416.m01141 DEAD/DEAH box helicase, putative similar
           to RNA helicase GB:A57514 GI:897915 from [Rattus
           norvegicus]; contains Pfam profiles PF00270: DEAD/DEAH
           box helicase, PF00271: Helicase conserved C-terminal
           domain
          Length = 989

 Score = 27.1 bits (57), Expect = 9.9
 Identities = 10/25 (40%), Positives = 16/25 (64%)
 Frame = -2

Query: 212 CVQQKRITYLCTSPGKIRHQRKISF 138
           C   + I  LCTS GKI + R++++
Sbjct: 525 CTPGRMIDILCTSSGKITNLRRVTY 549


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,507,506
Number of Sequences: 28952
Number of extensions: 286732
Number of successful extensions: 657
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 644
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 655
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1236350304
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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