BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11j08f (613 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 144 7e-36 SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 139 3e-34 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 137 1e-33 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 134 1e-32 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 127 1e-30 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 115 5e-27 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 82 7e-17 SPBC2A9.10 |||Bin3 family|Schizosaccharomyces pombe|chr 2|||Manual 28 1.2 SPAC56F8.09 |rrp8||rRNA methyltransferase Rrp8 |Schizosaccharomy... 27 2.1 SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 26 4.9 SPAC18G6.06 |||U3 snoRNP-associated protein Utp11|Schizosaccharo... 26 4.9 SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 26 4.9 SPBC543.10 |||GET complex subunit |Schizosaccharomyces pombe|chr... 25 6.5 SPAC20G8.02 |||phospholipase|Schizosaccharomyces pombe|chr 1|||M... 25 8.6 SPBC17D1.02 |||diphthamide biosynthesis protein |Schizosaccharom... 25 8.6 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 144 bits (350), Expect = 7e-36 Identities = 72/168 (42%), Positives = 106/168 (63%) Frame = +1 Query: 109 IGIDFGNESCYIAVAKAGGIETIANDYSLRGTPSCVAFSQKNRILGVAAKNQMVTNMKNT 288 IGID G +AV + +E I ND R TPS VAF++ R++G AAKNQ N +NT Sbjct: 9 IGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRNT 68 Query: 289 VFGFKRLLGRKFSDPLVQKELKHFPFKVEQRSDGGIGIRVNYLNEDNVFTPEQITAMLLT 468 VF KRL+GR++ DP QK++KH+PFKV ++G I VNYL E FT ++I+AM+LT Sbjct: 69 VFDAKRLIGRRYEDPETQKDIKHWPFKVID-NNGIPTIEVNYLGEKKQFTAQEISAMVLT 127 Query: 469 KLKDVAATALQTQINDCVISVPSYFTNAERXXXXXXXXXXXXXVLRLM 612 K+K+++ L ++ VI+VP+YF++++R VLR++ Sbjct: 128 KMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGLNVLRII 175 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 139 bits (336), Expect = 3e-34 Identities = 70/168 (41%), Positives = 101/168 (60%) Frame = +1 Query: 109 IGIDFGNESCYIAVAKAGGIETIANDYSLRGTPSCVAFSQKNRILGVAAKNQMVTNMKNT 288 IGID G + +E IAND R TPS VAF+ R++G AAKNQ+ N NT Sbjct: 5 IGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHNT 64 Query: 289 VFGFKRLLGRKFSDPLVQKELKHFPFKVEQRSDGGIGIRVNYLNEDNVFTPEQITAMLLT 468 +F KRL+GRKF DP VQ ++KH+PFKV + DG ++V Y E FTPE+I++M+L Sbjct: 65 IFDAKRLIGRKFDDPEVQSDMKHWPFKVISK-DGKPVLQVEYKGETKTFTPEEISSMVLM 123 Query: 469 KLKDVAATALQTQINDCVISVPSYFTNAERXXXXXXXXXXXXXVLRLM 612 K+++ A L ++ D V++VP+YF +++R VLR++ Sbjct: 124 KMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRII 171 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 137 bits (331), Expect = 1e-33 Identities = 69/168 (41%), Positives = 103/168 (61%) Frame = +1 Query: 109 IGIDFGNESCYIAVAKAGGIETIANDYSLRGTPSCVAFSQKNRILGVAAKNQMVTNMKNT 288 IGID G + +E IAND R TPS VAF+ R++G AAKNQ+ N NT Sbjct: 5 IGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHNT 64 Query: 289 VFGFKRLLGRKFSDPLVQKELKHFPFKVEQRSDGGIGIRVNYLNEDNVFTPEQITAMLLT 468 +F KRL+GR+F+DP VQ ++KH+PFKV ++ DG I+V + E FTPE+I++M+L Sbjct: 65 IFDAKRLIGRRFNDPEVQSDMKHWPFKVIEK-DGKPLIQVEFKGETKTFTPEEISSMVLL 123 Query: 469 KLKDVAATALQTQINDCVISVPSYFTNAERXXXXXXXXXXXXXVLRLM 612 K+++ A L ++ D V++VP+YF +++R VLR++ Sbjct: 124 KMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRII 171 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 134 bits (323), Expect = 1e-32 Identities = 68/170 (40%), Positives = 102/170 (60%) Frame = +1 Query: 103 SVIGIDFGNESCYIAVAKAGGIETIANDYSLRGTPSCVAFSQKNRILGVAAKNQMVTNMK 282 +VIGID G +AV K G +E IAND R TPS VAF++ R++G AAKNQ +N + Sbjct: 36 TVIGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPE 95 Query: 283 NTVFGFKRLLGRKFSDPLVQKELKHFPFKVEQRSDGGIGIRVNYLNEDNVFTPEQITAML 462 NT+F KRL+GRKF + + K++K FPF + + + + VN + FTPE+I+AM+ Sbjct: 96 NTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPL-VEVNVGGKKKKFTPEEISAMI 154 Query: 463 LTKLKDVAATALQTQINDCVISVPSYFTNAERXXXXXXXXXXXXXVLRLM 612 L+K+K A L + V++VP+YF +A+R V+R++ Sbjct: 155 LSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIV 204 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 127 bits (307), Expect = 1e-30 Identities = 66/170 (38%), Positives = 97/170 (57%) Frame = +1 Query: 103 SVIGIDFGNESCYIAVAKAGGIETIANDYSLRGTPSCVAFSQKNRILGVAAKNQMVTNMK 282 +V+GIDFGN IAVA+ I+ I N+ S R TPS V++ +++R LG AAK+ +N + Sbjct: 6 NVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEASNFR 65 Query: 283 NTVFGFKRLLGRKFSDPLVQKELKHFPFKVEQRSDGGIGIRVNYLNEDNVFTPEQITAML 462 NTV KRL GR + DP ++ +F DG +G +V YLNE+ F+ Q+ A Sbjct: 66 NTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAY 125 Query: 463 LTKLKDVAATALQTQINDCVISVPSYFTNAERXXXXXXXXXXXXXVLRLM 612 TK+K +A L ++D VIS+P++FT+ +R LRLM Sbjct: 126 FTKIKAIAEAELIGSVSDVVISIPAWFTDIQRRALLEAANIAGLNPLRLM 175 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 115 bits (277), Expect = 5e-27 Identities = 59/170 (34%), Positives = 99/170 (58%), Gaps = 1/170 (0%) Frame = +1 Query: 106 VIGIDFGNESCYIAVAKAGGIETIANDYSLRGTPSCVAFSQKN-RILGVAAKNQMVTNMK 282 VIGID G + +A+ + + IAN R TPS VAF++ R++GV+AK Q V N + Sbjct: 52 VIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVINPE 111 Query: 283 NTVFGFKRLLGRKFSDPLVQKELKHFPFKVEQRSDGGIGIRVNYLNEDNVFTPEQITAML 462 NT F KRL+GR+F +P VQ+++K P+K+ + S+G + ++P QI + Sbjct: 112 NTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGDAWLEA----RGKTYSPSQIGGFI 167 Query: 463 LTKLKDVAATALQTQINDCVISVPSYFTNAERXXXXXXXXXXXXXVLRLM 612 L+K+++ A+T L + + V++VP+YF +++R VLR++ Sbjct: 168 LSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAIAGLNVLRVV 217 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 81.8 bits (193), Expect = 7e-17 Identities = 55/174 (31%), Positives = 83/174 (47%), Gaps = 4/174 (2%) Frame = +1 Query: 103 SVIGIDFGNESCYIAVAKAGGIETIANDYSLRGTPSCVAFSQKNRILGVAAKNQMVTNMK 282 +VIGI FGN++ IA + G + +AN+ R PS +++ GV A+ Q+V N Sbjct: 25 TVIGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNAD 84 Query: 283 NTVFGFKRLLGRKFSDPLVQKELKHFPFKVEQRSDGGIGIRVNYLNE----DNVFTPEQI 450 N+V F+ LLG K D L + V G I V E + + T + Sbjct: 85 NSVTNFRDLLG-KSHDELTLHHCHYSANPVNVEGQIGFKITVQEDEESDPKEKILTAHEA 143 Query: 451 TAMLLTKLKDVAATALQTQINDCVISVPSYFTNAERXXXXXXXXXXXXXVLRLM 612 + L +L + A L T++N CV+SVP YFT+A+R VL+L+ Sbjct: 144 SVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESAANEAGLPVLQLI 197 >SPBC2A9.10 |||Bin3 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 268 Score = 27.9 bits (59), Expect = 1.2 Identities = 22/81 (27%), Positives = 34/81 (41%) Frame = +1 Query: 124 GNESCYIAVAKAGGIETIANDYSLRGTPSCVAFSQKNRILGVAAKNQMVTNMKNTVFGFK 303 GN Y ++ GG I D L+ P + + +L + N V+ ++FG Sbjct: 7 GNYHSYYSMR--GGTSII--DPRLKCLPDSLFYEAS--VLDIGCNNGTVSAQIASIFGAS 60 Query: 304 RLLGRKFSDPLVQKELKHFPF 366 +LG L+QK KH F Sbjct: 61 FVLGLDIDHVLIQKARKHLEF 81 >SPAC56F8.09 |rrp8||rRNA methyltransferase Rrp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 318 Score = 27.1 bits (57), Expect = 2.1 Identities = 20/81 (24%), Positives = 40/81 (49%), Gaps = 2/81 (2%) Frame = +1 Query: 73 ENLSITMAAMSVIGIDFGN--ESCYIAVAKAGGIETIANDYSLRGTPSCVAFSQKNRILG 246 E + I + +S++G ++ + + Y + K GG+ +A S S F+++ LG Sbjct: 218 ETVDIAVFCLSLMGTNWQSFLKEAY-RILKVGGLLWVAEIKSRFSDKSGEVFAKELPKLG 276 Query: 247 VAAKNQMVTNMKNTVFGFKRL 309 K+ + N T+F FK++ Sbjct: 277 FETKSIQLQNKMFTLFEFKKV 297 >SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9|Schizosaccharomyces pombe|chr 1|||Manual Length = 1223 Score = 25.8 bits (54), Expect = 4.9 Identities = 11/28 (39%), Positives = 15/28 (53%) Frame = +3 Query: 459 VVDKTKRCCSNSFTDSNQRLCDICSQLF 542 V D KR N F D + R+C I + +F Sbjct: 889 VKDSIKRLGDNKFEDKSLRICTIPNSIF 916 >SPAC18G6.06 |||U3 snoRNP-associated protein Utp11|Schizosaccharomyces pombe|chr 1|||Manual Length = 249 Score = 25.8 bits (54), Expect = 4.9 Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 5/57 (8%) Frame = +1 Query: 343 KELKHF-PFKVEQRSDGGIGIRVNYLNED----NVFTPEQITAMLLTKLKDVAATAL 498 KE K F P + Q +D +G N + +D N T + + L +KLK+ AAT L Sbjct: 144 KEAKSFNPAEFFQTTDDLVGRTENRVKKDQIENNELTNQPFSGKLHSKLKEKAATEL 200 >SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces pombe|chr 2|||Manual Length = 568 Score = 25.8 bits (54), Expect = 4.9 Identities = 12/36 (33%), Positives = 17/36 (47%) Frame = +1 Query: 406 VNYLNEDNVFTPEQITAMLLTKLKDVAATALQTQIN 513 + YLNE N+ TPE + + A + QIN Sbjct: 417 IKYLNEHNILTPEGEQHKFGDDIAEAAERKMTDQIN 452 >SPBC543.10 |||GET complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 171 Score = 25.4 bits (53), Expect = 6.5 Identities = 15/48 (31%), Positives = 25/48 (52%) Frame = +1 Query: 364 FKVEQRSDGGIGIRVNYLNEDNVFTPEQITAMLLTKLKDVAATALQTQ 507 F ++ DG I V+ N ++ ++I LLT K++ AT+ Q Q Sbjct: 21 FAKKRTIDGAYRIYVSVSNNKDLKKNKEILEQLLTVKKELNATSSQDQ 68 >SPAC20G8.02 |||phospholipase|Schizosaccharomyces pombe|chr 1|||Manual Length = 757 Score = 25.0 bits (52), Expect = 8.6 Identities = 15/48 (31%), Positives = 24/48 (50%) Frame = +1 Query: 298 FKRLLGRKFSDPLVQKELKHFPFKVEQRSDGGIGIRVNYLNEDNVFTP 441 F+ L G+K + P+ + L EQ D G+ I ++ N N+F P Sbjct: 564 FQMLNGKKIAGPIPKTNLTRSLSYSEQSFDSGVSI-LSCQNFYNIFHP 610 >SPBC17D1.02 |||diphthamide biosynthesis protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 503 Score = 25.0 bits (52), Expect = 8.6 Identities = 14/39 (35%), Positives = 20/39 (51%) Frame = -3 Query: 131 SLPKSIPMTDIAAIVIDKFSYFTEKILNYKYLLIN*VIS 15 SLPKS+ + D+ + I S IL Y L+N +S Sbjct: 204 SLPKSLSLQDMTLLYIGPDSPTLSSILMSHYSLVNQFLS 242 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,531,697 Number of Sequences: 5004 Number of extensions: 54236 Number of successful extensions: 127 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 117 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 119 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 267622334 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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