SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV11j07f
         (582 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc...   111   9e-26
SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac...   106   2e-24
SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3 |Schizosacc...    41   1e-04
SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc...    35   0.008
SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyc...    32   0.053
SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces...    31   0.093
SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual      28   0.87 
SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3 |Schizosac...    27   2.0  
SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p...    27   2.6  
SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ...    27   2.6  
SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces pom...    26   3.5  
SPCC320.11c ||SPCC330.18|RNA-binding protein|Schizosaccharomyces...    26   3.5  
SPAC1002.05c |jmj2||histone demethylase Jmj2 |Schizosaccharomyce...    26   3.5  
SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA reductase|Schi...    26   3.5  
SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pm...    26   3.5  
SPAC824.09c |||GTPase activating protein |Schizosaccharomyces po...    26   4.6  
SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosacc...    26   4.6  
SPCC1259.11c |gyp2||GTPase activating protein Gyp2 |Schizosaccha...    25   6.1  
SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosac...    25   8.1  
SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosacchar...    25   8.1  
SPCC1235.07 |fta7|sma7, cnl3|Sim4 and Mal2 associated |Schizosac...    25   8.1  

>SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 426

 Score =  111 bits (266), Expect = 9e-26
 Identities = 55/137 (40%), Positives = 87/137 (63%), Gaps = 2/137 (1%)
 Frame = +1

Query: 148 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRV--NVIHARQQVRSPVTNIARTSFFHI 321
           M   +FV N KG+V+ISR YR DI  + V+ F    + +   Q   +P       ++ +I
Sbjct: 1   MASAIFVLNLKGKVIISRDYRADIPMSVVEKFLPLKSEVEEEQGFSTPCLTHEGINYIYI 60

Query: 322 KRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDF 501
              +++L A++K N +A  +  FL K+ DV   YF ++ EE+I++NFVL+YELLDEI+DF
Sbjct: 61  HHNDVYLLALSKMNSDAMEMLVFLRKMADVFIDYFKELQEESIRDNFVLVYELLDEIMDF 120

Query: 502 GYPQNSDTGVLKTFITQ 552
           G+PQ ++T +L+ +ITQ
Sbjct: 121 GFPQTTETKILQEYITQ 137


>SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 446

 Score =  106 bits (255), Expect = 2e-24
 Identities = 46/129 (35%), Positives = 81/129 (62%)
 Frame = +1

Query: 148 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHIKR 327
           MI GLF++N KG+ LI + +R D+ ++  + FRV ++      R P+ +I  +++ + K 
Sbjct: 1   MISGLFIFNLKGDTLICKTFRHDLKKSVTEIFRVAIL-TNTDYRHPIVSIGSSTYIYTKH 59

Query: 328 ANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGY 507
            ++++ A+TK N N  +V EFL  +I  +  YFGK++E  +K+N   I+ELLDE++D+G 
Sbjct: 60  EDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTVKDNVSFIFELLDEMIDYGI 119

Query: 508 PQNSDTGVL 534
            Q ++   L
Sbjct: 120 IQTTEPDAL 128


>SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 425

 Score = 41.1 bits (92), Expect = 1e-04
 Identities = 16/72 (22%), Positives = 39/72 (54%)
 Frame = +1

Query: 331 NIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGYP 510
           ++ L   T  +     + + + +I+DV++++FG  +   ++ N  +I +LL E++D+GY 
Sbjct: 66  DVRLCIPTTCDTEPLYIHDIMRRIVDVVKTFFGGFNASKVEKNVCVIVQLLAEMIDYGYA 125

Query: 511 QNSDTGVLKTFI 546
              +   L+  +
Sbjct: 126 TCMEPNALQDIV 137


>SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 143

 Score = 35.1 bits (77), Expect = 0.008
 Identities = 25/119 (21%), Positives = 54/119 (45%), Gaps = 3/119 (2%)
 Frame = +1

Query: 148 MIGGLFVYNHKGEVLISRVYR--DDIGRNAVDAFRVNVIHAR-QQVRSPVTNIARTSFFH 318
           MI  + + N  G+  +S+ Y   DD  +  + A    +I  R Q+ ++       +   +
Sbjct: 1   MIQFILIQNRHGKNRLSKYYVPFDDDEKVRLKARIHQLISQRNQKFQANFLEWENSKLVY 60

Query: 319 IKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEIL 495
            + A ++         N   + E +   ++++ S+FG + E ++  NF  +  +LDEI+
Sbjct: 61  RRYAGLYFCFCVDSTDNDLAILEMIHFFVEILDSFFGNVCELDLIFNFYKVSAILDEII 119


>SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 240

 Score = 32.3 bits (70), Expect = 0.053
 Identities = 27/132 (20%), Positives = 54/132 (40%), Gaps = 2/132 (1%)
 Frame = +1

Query: 166 VYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTS--FFHIKRANIW 339
           + N  G+ +ISR +R+ + R  V++  ++   A    +S  T +   +  F +     ++
Sbjct: 8   IVNRGGKAIISRQFRE-MSRVRVESL-LSSFPALVSEKSQNTTVESDNVRFVYQPLDELY 65

Query: 340 LAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGYPQNS 519
           +  +T    N     + L  +  V+ S    + E  I      I+   DE    GY  N 
Sbjct: 66  IVLITNLQSNILQDIDTLHLLSQVVTSICSSLEEREILEYAFEIFTAFDEATSLGYRDNV 125

Query: 520 DTGVLKTFITQQ 555
               +KT++  +
Sbjct: 126 SLTQIKTYLEME 137


>SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 879

 Score = 31.5 bits (68), Expect = 0.093
 Identities = 17/55 (30%), Positives = 28/55 (50%)
 Frame = -3

Query: 517 NSEGNQSPRSHQATHRSEQSYS*CSLQKSCQNTIASHQ*SSTGTQIPWPRSHFAL 353
           +S   +  RS  AT +S++S S   +  S   T  S+   ++ TQ+ WP +H  L
Sbjct: 719 DSGSRRVTRSQNATSQSQESGS--EIGSSIAGTAGSYNVGTSNTQLSWPSTHSTL 771


>SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 483

 Score = 28.3 bits (60), Expect = 0.87
 Identities = 12/46 (26%), Positives = 29/46 (63%)
 Frame = +1

Query: 316 HIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIK 453
           +I+  N  + +  +++ + A++ E   +++DV QS+ G + EEN++
Sbjct: 414 NIEITNPIIVSPVERSRSQALLNEIQTRVLDVRQSHGGVVLEENVR 459


>SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 165

 Score = 27.1 bits (57), Expect = 2.0
 Identities = 21/127 (16%), Positives = 55/127 (43%), Gaps = 8/127 (6%)
 Frame = +1

Query: 148 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPV------TNI--AR 303
           MI  +F++N+KG+  +++ Y       ++    +  I+A    R P       +N+   +
Sbjct: 1   MIYAVFIFNNKGKPRLTKFYTPI--DESIQQKLIGDIYAAVSTRPPTACNFLESNLIAGK 58

Query: 304 TSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELL 483
               + + A ++   V  +  +   + + +   ++ +   F  + E ++   F  I+ +L
Sbjct: 59  NRIIYRQYATLYFVFVVDEGESELGILDLIQVFVEALDRCFNNVCELDLVFKFQEIHAIL 118

Query: 484 DEILDFG 504
            E++  G
Sbjct: 119 AEVVSGG 125


>SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1040

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 12/29 (41%), Positives = 19/29 (65%)
 Frame = +3

Query: 90  SLNSNYIETNFERV*GLTYNDRRLVRLQP 176
           S + N +E+N E+V GL  ND  ++ L+P
Sbjct: 77  SSSLNTMESNIEKVNGLFPNDNSVIALKP 105


>SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1364

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 15/47 (31%), Positives = 22/47 (46%)
 Frame = +1

Query: 415  QSYFGKISEENIKNNFVLIYELLDEILDFGYPQNSDTGVLKTFITQQ 555
            + +  KI EE +K N +L YE      D     +S   ++KTF   Q
Sbjct: 952  KDFVTKIEEETLKENPLLFYEYRKASSDMRVLMDSQFLMMKTFARLQ 998


>SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1188

 Score = 26.2 bits (55), Expect = 3.5
 Identities = 23/102 (22%), Positives = 48/102 (47%), Gaps = 11/102 (10%)
 Frame = +1

Query: 238  AFRVNVIHARQQVRSPVTNIARTSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQ 417
            + RVN+   +   +S  +N + T+ +H++  +  +         +A+VFEF  K +    
Sbjct: 839  SIRVNIAGVKITAKSSTSNSSSTAEYHVRSRHAVIP--VNNRYRSAVVFEF-RKQLQRKH 895

Query: 418  SYFGKI----SEENIKNNF-VLIY------ELLDEILDFGYP 510
            + F  +     E+N++ N  V I+       +L  ++DF +P
Sbjct: 896  NVFAMVWLVDLEDNVEQNIRVPIFTSSKPAHVLQNMIDFDHP 937


>SPCC320.11c ||SPCC330.18|RNA-binding protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 180

 Score = 26.2 bits (55), Expect = 3.5
 Identities = 11/37 (29%), Positives = 23/37 (62%)
 Frame = +1

Query: 409 VMQSYFGKISEENIKNNFVLIYELLDEILDFGYPQNS 519
           V++++ G+I+++  ++  V+IY + D  L FG    S
Sbjct: 113 VLKAHVGRITDDTPQHQGVVIYSMNDTPLGFGVTARS 149


>SPAC1002.05c |jmj2||histone demethylase Jmj2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 715

 Score = 26.2 bits (55), Expect = 3.5
 Identities = 8/24 (33%), Positives = 13/24 (54%)
 Frame = -1

Query: 354 CDCCQPNICTLDMEERCACNVGNR 283
           C C    +C L +E  C C++ N+
Sbjct: 675 CSCKSITVCPLHIEYLCKCDLSNK 698


>SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA
           reductase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1053

 Score = 26.2 bits (55), Expect = 3.5
 Identities = 9/40 (22%), Positives = 25/40 (62%)
 Frame = -1

Query: 576 LGGRXDALLSDECLQDPSIRILRVTKVQDLIKQLIDQNKV 457
           + GR  +++++  +   +++ +  T V+DL+K  +D+N +
Sbjct: 854 IDGRGKSVIAEAIIPGDAVKSVLKTTVEDLVKLNVDKNLI 893


>SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pmc1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1292

 Score = 26.2 bits (55), Expect = 3.5
 Identities = 17/54 (31%), Positives = 24/54 (44%)
 Frame = -3

Query: 538 SSGPQYQNSEGNQSPRSHQATHRSEQSYS*CSLQKSCQNTIASHQ*SSTGTQIP 377
           SS P   +SE NQ P S Q       +Y+         ++  S + SS+GT  P
Sbjct: 10  SSRPPSVHSERNQKPSSSQFLGVPSSNYNQRENSSRSGSSTISREPSSSGTMYP 63


>SPAC824.09c |||GTPase activating protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 320

 Score = 25.8 bits (54), Expect = 4.6
 Identities = 11/34 (32%), Positives = 18/34 (52%)
 Frame = +2

Query: 272 RCDHLLPTLHAHLSSISSVQIFGWQQSQSKM*TR 373
           RC  +  +L  H+S + SV +  W   Q++  TR
Sbjct: 51  RCSGVHRSLGVHVSRVKSVDLDSWTDEQTENMTR 84


>SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 878

 Score = 25.8 bits (54), Expect = 4.6
 Identities = 16/58 (27%), Positives = 28/58 (48%)
 Frame = +1

Query: 64  YVKFLKGCNR*IAITLKPILKECEASPTMIGGLFVYNHKGEVLISRVYRDDIGRNAVD 237
           +V ++ G  R I + L P+L      PT+      +NH G++ + R  +   G N+ D
Sbjct: 737 HVSYVTGVIRSIDLQL-PVLLSKFMKPTIFDSYDFFNHWGQMGVEREAQLTFGLNSKD 793


>SPCC1259.11c |gyp2||GTPase activating protein Gyp2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 720

 Score = 25.4 bits (53), Expect = 6.1
 Identities = 11/21 (52%), Positives = 13/21 (61%)
 Frame = +2

Query: 275 CDHLLPTLHAHLSSISSVQIF 337
           CD L+  LHA L   SSV +F
Sbjct: 118 CDRLVRQLHASLEDASSVGLF 138


>SPBC3H7.15 |hhp1||serine/threonine protein kinase
           Hhp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 365

 Score = 25.0 bits (52), Expect = 8.1
 Identities = 11/30 (36%), Positives = 16/30 (53%)
 Frame = +3

Query: 294 HCTHIFLPYQACKYLAGSSHKAKCERGHGI 383
           H TH+ +PY+  K L G++  A      GI
Sbjct: 161 HKTHLHIPYRENKNLTGTARYASINTHLGI 190


>SPAC6C3.06c |||P-type ATPase, calcium
            transporting|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1033

 Score = 25.0 bits (52), Expect = 8.1
 Identities = 18/77 (23%), Positives = 31/77 (40%), Gaps = 1/77 (1%)
 Frame = +1

Query: 211  DDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHIKRANIWLAAVT-KQNVNAAMVFE 387
            +D+G   V    + ++    Q  S   + +   F H+ R  +W   ++ KQ    AM   
Sbjct: 773  NDVGMIQVANVGIGIVGKEGQQASLAADYSVKEFSHVSRLLLWHGRISYKQTSKLAMFVI 832

Query: 388  FLLKIIDVMQSYFGKIS 438
                +I V Q  +  IS
Sbjct: 833  HRGLLISVCQVVYSVIS 849


>SPCC1235.07 |fta7|sma7, cnl3|Sim4 and Mal2 associated
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 244

 Score = 25.0 bits (52), Expect = 8.1
 Identities = 17/48 (35%), Positives = 24/48 (50%)
 Frame = -1

Query: 525 SIRILRVTKVQDLIKQLIDQNKVILDVLFRNLAKIRLHHINDLQQELK 382
           ++ + R  K   L  QL+  N  +   L+ +L      HIN LQQELK
Sbjct: 71  NLPVPRKRKESILFSQLLSSNMQLEQQLYSDL-----DHINVLQQELK 113


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,420,990
Number of Sequences: 5004
Number of extensions: 49874
Number of successful extensions: 148
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 250133048
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -