BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11j07f (582 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc... 111 9e-26 SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac... 106 2e-24 SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3 |Schizosacc... 41 1e-04 SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc... 35 0.008 SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyc... 32 0.053 SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 31 0.093 SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual 28 0.87 SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3 |Schizosac... 27 2.0 SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p... 27 2.6 SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ... 27 2.6 SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces pom... 26 3.5 SPCC320.11c ||SPCC330.18|RNA-binding protein|Schizosaccharomyces... 26 3.5 SPAC1002.05c |jmj2||histone demethylase Jmj2 |Schizosaccharomyce... 26 3.5 SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA reductase|Schi... 26 3.5 SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pm... 26 3.5 SPAC824.09c |||GTPase activating protein |Schizosaccharomyces po... 26 4.6 SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosacc... 26 4.6 SPCC1259.11c |gyp2||GTPase activating protein Gyp2 |Schizosaccha... 25 6.1 SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosac... 25 8.1 SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosacchar... 25 8.1 SPCC1235.07 |fta7|sma7, cnl3|Sim4 and Mal2 associated |Schizosac... 25 8.1 >SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 111 bits (266), Expect = 9e-26 Identities = 55/137 (40%), Positives = 87/137 (63%), Gaps = 2/137 (1%) Frame = +1 Query: 148 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRV--NVIHARQQVRSPVTNIARTSFFHI 321 M +FV N KG+V+ISR YR DI + V+ F + + Q +P ++ +I Sbjct: 1 MASAIFVLNLKGKVIISRDYRADIPMSVVEKFLPLKSEVEEEQGFSTPCLTHEGINYIYI 60 Query: 322 KRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDF 501 +++L A++K N +A + FL K+ DV YF ++ EE+I++NFVL+YELLDEI+DF Sbjct: 61 HHNDVYLLALSKMNSDAMEMLVFLRKMADVFIDYFKELQEESIRDNFVLVYELLDEIMDF 120 Query: 502 GYPQNSDTGVLKTFITQ 552 G+PQ ++T +L+ +ITQ Sbjct: 121 GFPQTTETKILQEYITQ 137 >SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 446 Score = 106 bits (255), Expect = 2e-24 Identities = 46/129 (35%), Positives = 81/129 (62%) Frame = +1 Query: 148 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHIKR 327 MI GLF++N KG+ LI + +R D+ ++ + FRV ++ R P+ +I +++ + K Sbjct: 1 MISGLFIFNLKGDTLICKTFRHDLKKSVTEIFRVAIL-TNTDYRHPIVSIGSSTYIYTKH 59 Query: 328 ANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGY 507 ++++ A+TK N N +V EFL +I + YFGK++E +K+N I+ELLDE++D+G Sbjct: 60 EDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTVKDNVSFIFELLDEMIDYGI 119 Query: 508 PQNSDTGVL 534 Q ++ L Sbjct: 120 IQTTEPDAL 128 >SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 425 Score = 41.1 bits (92), Expect = 1e-04 Identities = 16/72 (22%), Positives = 39/72 (54%) Frame = +1 Query: 331 NIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGYP 510 ++ L T + + + + +I+DV++++FG + ++ N +I +LL E++D+GY Sbjct: 66 DVRLCIPTTCDTEPLYIHDIMRRIVDVVKTFFGGFNASKVEKNVCVIVQLLAEMIDYGYA 125 Query: 511 QNSDTGVLKTFI 546 + L+ + Sbjct: 126 TCMEPNALQDIV 137 >SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 143 Score = 35.1 bits (77), Expect = 0.008 Identities = 25/119 (21%), Positives = 54/119 (45%), Gaps = 3/119 (2%) Frame = +1 Query: 148 MIGGLFVYNHKGEVLISRVYR--DDIGRNAVDAFRVNVIHAR-QQVRSPVTNIARTSFFH 318 MI + + N G+ +S+ Y DD + + A +I R Q+ ++ + + Sbjct: 1 MIQFILIQNRHGKNRLSKYYVPFDDDEKVRLKARIHQLISQRNQKFQANFLEWENSKLVY 60 Query: 319 IKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEIL 495 + A ++ N + E + ++++ S+FG + E ++ NF + +LDEI+ Sbjct: 61 RRYAGLYFCFCVDSTDNDLAILEMIHFFVEILDSFFGNVCELDLIFNFYKVSAILDEII 119 >SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 240 Score = 32.3 bits (70), Expect = 0.053 Identities = 27/132 (20%), Positives = 54/132 (40%), Gaps = 2/132 (1%) Frame = +1 Query: 166 VYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTS--FFHIKRANIW 339 + N G+ +ISR +R+ + R V++ ++ A +S T + + F + ++ Sbjct: 8 IVNRGGKAIISRQFRE-MSRVRVESL-LSSFPALVSEKSQNTTVESDNVRFVYQPLDELY 65 Query: 340 LAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGYPQNS 519 + +T N + L + V+ S + E I I+ DE GY N Sbjct: 66 IVLITNLQSNILQDIDTLHLLSQVVTSICSSLEEREILEYAFEIFTAFDEATSLGYRDNV 125 Query: 520 DTGVLKTFITQQ 555 +KT++ + Sbjct: 126 SLTQIKTYLEME 137 >SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces pombe|chr 3|||Manual Length = 879 Score = 31.5 bits (68), Expect = 0.093 Identities = 17/55 (30%), Positives = 28/55 (50%) Frame = -3 Query: 517 NSEGNQSPRSHQATHRSEQSYS*CSLQKSCQNTIASHQ*SSTGTQIPWPRSHFAL 353 +S + RS AT +S++S S + S T S+ ++ TQ+ WP +H L Sbjct: 719 DSGSRRVTRSQNATSQSQESGS--EIGSSIAGTAGSYNVGTSNTQLSWPSTHSTL 771 >SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual Length = 483 Score = 28.3 bits (60), Expect = 0.87 Identities = 12/46 (26%), Positives = 29/46 (63%) Frame = +1 Query: 316 HIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIK 453 +I+ N + + +++ + A++ E +++DV QS+ G + EEN++ Sbjct: 414 NIEITNPIIVSPVERSRSQALLNEIQTRVLDVRQSHGGVVLEENVR 459 >SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 165 Score = 27.1 bits (57), Expect = 2.0 Identities = 21/127 (16%), Positives = 55/127 (43%), Gaps = 8/127 (6%) Frame = +1 Query: 148 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPV------TNI--AR 303 MI +F++N+KG+ +++ Y ++ + I+A R P +N+ + Sbjct: 1 MIYAVFIFNNKGKPRLTKFYTPI--DESIQQKLIGDIYAAVSTRPPTACNFLESNLIAGK 58 Query: 304 TSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELL 483 + + A ++ V + + + + + ++ + F + E ++ F I+ +L Sbjct: 59 NRIIYRQYATLYFVFVVDEGESELGILDLIQVFVEALDRCFNNVCELDLVFKFQEIHAIL 118 Query: 484 DEILDFG 504 E++ G Sbjct: 119 AEVVSGG 125 >SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1040 Score = 26.6 bits (56), Expect = 2.6 Identities = 12/29 (41%), Positives = 19/29 (65%) Frame = +3 Query: 90 SLNSNYIETNFERV*GLTYNDRRLVRLQP 176 S + N +E+N E+V GL ND ++ L+P Sbjct: 77 SSSLNTMESNIEKVNGLFPNDNSVIALKP 105 >SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces pombe|chr 3|||Manual Length = 1364 Score = 26.6 bits (56), Expect = 2.6 Identities = 15/47 (31%), Positives = 22/47 (46%) Frame = +1 Query: 415 QSYFGKISEENIKNNFVLIYELLDEILDFGYPQNSDTGVLKTFITQQ 555 + + KI EE +K N +L YE D +S ++KTF Q Sbjct: 952 KDFVTKIEEETLKENPLLFYEYRKASSDMRVLMDSQFLMMKTFARLQ 998 >SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 1188 Score = 26.2 bits (55), Expect = 3.5 Identities = 23/102 (22%), Positives = 48/102 (47%), Gaps = 11/102 (10%) Frame = +1 Query: 238 AFRVNVIHARQQVRSPVTNIARTSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQ 417 + RVN+ + +S +N + T+ +H++ + + +A+VFEF K + Sbjct: 839 SIRVNIAGVKITAKSSTSNSSSTAEYHVRSRHAVIP--VNNRYRSAVVFEF-RKQLQRKH 895 Query: 418 SYFGKI----SEENIKNNF-VLIY------ELLDEILDFGYP 510 + F + E+N++ N V I+ +L ++DF +P Sbjct: 896 NVFAMVWLVDLEDNVEQNIRVPIFTSSKPAHVLQNMIDFDHP 937 >SPCC320.11c ||SPCC330.18|RNA-binding protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 180 Score = 26.2 bits (55), Expect = 3.5 Identities = 11/37 (29%), Positives = 23/37 (62%) Frame = +1 Query: 409 VMQSYFGKISEENIKNNFVLIYELLDEILDFGYPQNS 519 V++++ G+I+++ ++ V+IY + D L FG S Sbjct: 113 VLKAHVGRITDDTPQHQGVVIYSMNDTPLGFGVTARS 149 >SPAC1002.05c |jmj2||histone demethylase Jmj2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 715 Score = 26.2 bits (55), Expect = 3.5 Identities = 8/24 (33%), Positives = 13/24 (54%) Frame = -1 Query: 354 CDCCQPNICTLDMEERCACNVGNR 283 C C +C L +E C C++ N+ Sbjct: 675 CSCKSITVCPLHIEYLCKCDLSNK 698 >SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA reductase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1053 Score = 26.2 bits (55), Expect = 3.5 Identities = 9/40 (22%), Positives = 25/40 (62%) Frame = -1 Query: 576 LGGRXDALLSDECLQDPSIRILRVTKVQDLIKQLIDQNKV 457 + GR +++++ + +++ + T V+DL+K +D+N + Sbjct: 854 IDGRGKSVIAEAIIPGDAVKSVLKTTVEDLVKLNVDKNLI 893 >SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pmc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1292 Score = 26.2 bits (55), Expect = 3.5 Identities = 17/54 (31%), Positives = 24/54 (44%) Frame = -3 Query: 538 SSGPQYQNSEGNQSPRSHQATHRSEQSYS*CSLQKSCQNTIASHQ*SSTGTQIP 377 SS P +SE NQ P S Q +Y+ ++ S + SS+GT P Sbjct: 10 SSRPPSVHSERNQKPSSSQFLGVPSSNYNQRENSSRSGSSTISREPSSSGTMYP 63 >SPAC824.09c |||GTPase activating protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 320 Score = 25.8 bits (54), Expect = 4.6 Identities = 11/34 (32%), Positives = 18/34 (52%) Frame = +2 Query: 272 RCDHLLPTLHAHLSSISSVQIFGWQQSQSKM*TR 373 RC + +L H+S + SV + W Q++ TR Sbjct: 51 RCSGVHRSLGVHVSRVKSVDLDSWTDEQTENMTR 84 >SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 878 Score = 25.8 bits (54), Expect = 4.6 Identities = 16/58 (27%), Positives = 28/58 (48%) Frame = +1 Query: 64 YVKFLKGCNR*IAITLKPILKECEASPTMIGGLFVYNHKGEVLISRVYRDDIGRNAVD 237 +V ++ G R I + L P+L PT+ +NH G++ + R + G N+ D Sbjct: 737 HVSYVTGVIRSIDLQL-PVLLSKFMKPTIFDSYDFFNHWGQMGVEREAQLTFGLNSKD 793 >SPCC1259.11c |gyp2||GTPase activating protein Gyp2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 720 Score = 25.4 bits (53), Expect = 6.1 Identities = 11/21 (52%), Positives = 13/21 (61%) Frame = +2 Query: 275 CDHLLPTLHAHLSSISSVQIF 337 CD L+ LHA L SSV +F Sbjct: 118 CDRLVRQLHASLEDASSVGLF 138 >SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 365 Score = 25.0 bits (52), Expect = 8.1 Identities = 11/30 (36%), Positives = 16/30 (53%) Frame = +3 Query: 294 HCTHIFLPYQACKYLAGSSHKAKCERGHGI 383 H TH+ +PY+ K L G++ A GI Sbjct: 161 HKTHLHIPYRENKNLTGTARYASINTHLGI 190 >SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosaccharomyces pombe|chr 1|||Manual Length = 1033 Score = 25.0 bits (52), Expect = 8.1 Identities = 18/77 (23%), Positives = 31/77 (40%), Gaps = 1/77 (1%) Frame = +1 Query: 211 DDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHIKRANIWLAAVT-KQNVNAAMVFE 387 +D+G V + ++ Q S + + F H+ R +W ++ KQ AM Sbjct: 773 NDVGMIQVANVGIGIVGKEGQQASLAADYSVKEFSHVSRLLLWHGRISYKQTSKLAMFVI 832 Query: 388 FLLKIIDVMQSYFGKIS 438 +I V Q + IS Sbjct: 833 HRGLLISVCQVVYSVIS 849 >SPCC1235.07 |fta7|sma7, cnl3|Sim4 and Mal2 associated |Schizosaccharomyces pombe|chr 3|||Manual Length = 244 Score = 25.0 bits (52), Expect = 8.1 Identities = 17/48 (35%), Positives = 24/48 (50%) Frame = -1 Query: 525 SIRILRVTKVQDLIKQLIDQNKVILDVLFRNLAKIRLHHINDLQQELK 382 ++ + R K L QL+ N + L+ +L HIN LQQELK Sbjct: 71 NLPVPRKRKESILFSQLLSSNMQLEQQLYSDL-----DHINVLQQELK 113 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,420,990 Number of Sequences: 5004 Number of extensions: 49874 Number of successful extensions: 148 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 144 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 146 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 250133048 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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