Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11j07f
(582 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g46630.2 68418.m05740 clathrin adaptor complexes medium subun... 141 4e-34
At5g46630.1 68418.m05741 clathrin adaptor complexes medium subun... 141 4e-34
At4g24550.2 68417.m03519 clathrin adaptor complexes medium subun... 105 3e-23
At4g24550.1 68417.m03518 clathrin adaptor complexes medium subun... 105 3e-23
At1g60780.1 68414.m06842 clathrin adaptor complexes medium subun... 105 3e-23
At1g10730.1 68414.m01223 clathrin adaptor complexes medium subun... 104 4e-23
At1g56590.1 68414.m06508 clathrin adaptor complexes medium subun... 73 1e-13
At4g35410.2 68417.m05030 clathrin adaptor complex small chain fa... 42 4e-04
At2g17380.1 68415.m02007 clathrin assembly protein AP19 identica... 40 0.002
At5g23700.1 68418.m02778 hypothetical protein 30 0.98
At4g20060.1 68417.m02935 expressed protein ; expression support... 29 1.7
At2g19790.1 68415.m02312 clathrin adaptor complex small chain fa... 28 5.2
At5g01270.1 68418.m00036 double-stranded RNA-binding domain (DsR... 27 6.9
At2g42720.1 68415.m05291 F-box family protein contains F-box dom... 27 6.9
At5g40480.1 68418.m04909 expressed protein ; expression supporte... 27 9.1
At1g65440.1 68414.m07424 glycine-rich protein 27 9.1
>At5g46630.2 68418.m05740 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 441
Score = 141 bits (341), Expect = 4e-34
Identities = 63/143 (44%), Positives = 95/143 (66%), Gaps = 1/143 (0%)
Frame = +1
Query: 142 PTMIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHI 321
P ++ N +G+VLI+R YRDD+G N VDAFR +++ ++ PV I SF ++
Sbjct: 2 PVAASAIYFLNLRGDVLINRTYRDDVGGNMVDAFRTHIMQTKELGNCPVRQIGGCSFVYM 61
Query: 322 KRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYF-GKISEENIKNNFVLIYELLDEILD 498
+ +N+++ V N N A F+F+++ + + +SYF G E+ I+NNFVLIYELLDEI+D
Sbjct: 62 RISNVYIVIVVSSNANVACGFKFVVEAVALFKSYFGGAFDEDAIRNNFVLIYELLDEIMD 121
Query: 499 FGYPQNSDTGVLKTFITQQGIXS 567
FGYPQN +LK +ITQ+G+ S
Sbjct: 122 FGYPQNLSPEILKLYITQEGVRS 144
>At5g46630.1 68418.m05741 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 438
Score = 141 bits (341), Expect = 4e-34
Identities = 63/143 (44%), Positives = 95/143 (66%), Gaps = 1/143 (0%)
Frame = +1
Query: 142 PTMIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHI 321
P ++ N +G+VLI+R YRDD+G N VDAFR +++ ++ PV I SF ++
Sbjct: 2 PVAASAIYFLNLRGDVLINRTYRDDVGGNMVDAFRTHIMQTKELGNCPVRQIGGCSFVYM 61
Query: 322 KRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYF-GKISEENIKNNFVLIYELLDEILD 498
+ +N+++ V N N A F+F+++ + + +SYF G E+ I+NNFVLIYELLDEI+D
Sbjct: 62 RISNVYIVIVVSSNANVACGFKFVVEAVALFKSYFGGAFDEDAIRNNFVLIYELLDEIMD 121
Query: 499 FGYPQNSDTGVLKTFITQQGIXS 567
FGYPQN +LK +ITQ+G+ S
Sbjct: 122 FGYPQNLSPEILKLYITQEGVRS 144
>At4g24550.2 68417.m03519 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 451
Score = 105 bits (251), Expect = 3e-23
Identities = 48/141 (34%), Positives = 87/141 (61%), Gaps = 3/141 (2%)
Frame = +1
Query: 148 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRS---PVTNIARTSFFH 318
MI FV + +G+ ++ R YR ++ + + + F V ++ + P+ N+ ++FH
Sbjct: 2 MISQFFVLSQRGDNIVFRDYRAEVPKGSTETFFRKVKFWKEDGNAEAPPIFNVDGVNYFH 61
Query: 319 IKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILD 498
+K ++ A T+ NV+ ++V E L +I V++ Y G ++E++ + NFVL+YELLDE++D
Sbjct: 62 VKVVGLYFVATTRVNVSPSLVLELLQRIARVIKDYLGVLNEDSFRKNFVLVYELLDEVID 121
Query: 499 FGYPQNSDTGVLKTFITQQGI 561
FGY Q + T VLK++I + I
Sbjct: 122 FGYVQTTSTEVLKSYIFNEPI 142
>At4g24550.1 68417.m03518 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 380
Score = 105 bits (251), Expect = 3e-23
Identities = 48/141 (34%), Positives = 87/141 (61%), Gaps = 3/141 (2%)
Frame = +1
Query: 148 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRS---PVTNIARTSFFH 318
MI FV + +G+ ++ R YR ++ + + + F V ++ + P+ N+ ++FH
Sbjct: 2 MISQFFVLSQRGDNIVFRDYRAEVPKGSTETFFRKVKFWKEDGNAEAPPIFNVDGVNYFH 61
Query: 319 IKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILD 498
+K ++ A T+ NV+ ++V E L +I V++ Y G ++E++ + NFVL+YELLDE++D
Sbjct: 62 VKVVGLYFVATTRVNVSPSLVLELLQRIARVIKDYLGVLNEDSFRKNFVLVYELLDEVID 121
Query: 499 FGYPQNSDTGVLKTFITQQGI 561
FGY Q + T VLK++I + I
Sbjct: 122 FGYVQTTSTEVLKSYIFNEPI 142
>At1g60780.1 68414.m06842 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 428
Score = 105 bits (251), Expect = 3e-23
Identities = 48/131 (36%), Positives = 81/131 (61%), Gaps = 2/131 (1%)
Frame = +1
Query: 160 LFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHAR--QQVRSPVTNIARTSFFHIKRAN 333
LF+ + KG VL+ R YR D+ + F +I Q PV ++ ++ +N
Sbjct: 8 LFLLDIKGRVLVWRDYRGDVSAAQAERFFTKLIEKEGDSQSNDPVAYDNGVTYMFVQHSN 67
Query: 334 IWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGYPQ 513
++L ++QN NAA + FL +++DV + YF ++ EE++++NFV++YELLDE++DFGYPQ
Sbjct: 68 VYLMIASRQNCNAASLLFFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEMMDFGYPQ 127
Query: 514 NSDTGVLKTFI 546
++ +L FI
Sbjct: 128 YTEARILSEFI 138
>At1g10730.1 68414.m01223 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 428
Score = 104 bits (250), Expect = 4e-23
Identities = 49/131 (37%), Positives = 81/131 (61%), Gaps = 2/131 (1%)
Frame = +1
Query: 160 LFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHAR--QQVRSPVTNIARTSFFHIKRAN 333
LF+ + KG VL+ R YR D+ + F +I Q PV ++ ++ +N
Sbjct: 8 LFLLDIKGRVLVWRDYRGDVTAAQAERFFTKLIETEGDSQSNDPVAYDNGVTYMFVQHSN 67
Query: 334 IWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGYPQ 513
I+L ++QN NAA + FL +++DV + YF ++ EE++++NFV++YELLDE++DFGYPQ
Sbjct: 68 IYLMIASRQNCNAASLLFFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEMMDFGYPQ 127
Query: 514 NSDTGVLKTFI 546
++ +L FI
Sbjct: 128 FTEARILSEFI 138
>At1g56590.1 68414.m06508 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 415
Score = 72.9 bits (171), Expect = 1e-13
Identities = 41/142 (28%), Positives = 72/142 (50%), Gaps = 2/142 (1%)
Frame = +1
Query: 148 MIGGLFVYNHKGEVLISRVYRDD-IGRNAVDAFRVNVIHARQQVRS-PVTNIARTSFFHI 321
M+ +F+ + GEV++ + + R+ F I ++ PV F I
Sbjct: 1 MLQCIFLISDSGEVMLEKQLTGHRVDRSICAWFWDQYISQGDSFKALPVIASPTHYLFQI 60
Query: 322 KRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDF 501
R I A ++ + M EFL ++ DV+ Y G ++E+ IK+NF+++YELLDE++D
Sbjct: 61 VRDGITFLACSQVEMPPLMAIEFLCRVADVLSEYLGGLNEDLIKDNFIIVYELLDEMIDN 120
Query: 502 GYPQNSDTGVLKTFITQQGIXS 567
G+P ++ +LK I + S
Sbjct: 121 GFPLTTEPSILKEMIAPPNLVS 142
>At4g35410.2 68417.m05030 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 162
Score = 41.5 bits (93), Expect = 4e-04
Identities = 24/86 (27%), Positives = 41/86 (47%), Gaps = 1/86 (1%)
Frame = +1
Query: 328 ANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGY 507
A+++ Q N V E + ++++ YFG + E ++ NF Y +LDE+L G
Sbjct: 63 ASLYFCMCIDQEDNELEVLEIIHHYVEILDRYFGSVCELDLIFNFHKAYYILDELLIAGE 122
Query: 508 PQNSDTGVLKTFIT-QQGIXSASKEE 582
Q S + I+ Q + +KEE
Sbjct: 123 LQESSKKTVARIISAQDQLVEVAKEE 148
>At2g17380.1 68415.m02007 clathrin assembly protein AP19 identical
to clathrin assembly protein AP19 GI:2231698 from
[Arabidopsis thaliana]
Length = 161
Score = 39.5 bits (88), Expect = 0.002
Identities = 23/86 (26%), Positives = 41/86 (47%), Gaps = 1/86 (1%)
Frame = +1
Query: 328 ANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGY 507
A+++ + N V E + ++++ YFG + E ++ NF Y +LDE+L G
Sbjct: 63 ASLYFCMCIDEADNELEVLEIIHHYVEILDRYFGSVCELDLIFNFHKAYYILDELLIAGE 122
Query: 508 PQNSDTGVLKTFIT-QQGIXSASKEE 582
Q S + I+ Q + +KEE
Sbjct: 123 LQESSKKTVARIISAQDQLVEVAKEE 148
>At5g23700.1 68418.m02778 hypothetical protein
Length = 572
Score = 30.3 bits (65), Expect = 0.98
Identities = 16/46 (34%), Positives = 25/46 (54%), Gaps = 3/46 (6%)
Frame = -2
Query: 248 TLNASTALRPISSR*TLEINTSPLWL*TN---KPPIIVGEASHSFK 120
++ +S+ RP + + N SPLW+ KPP+I+ HSFK
Sbjct: 118 SVRSSSTGRPSTFSRSSTPNASPLWMPPKASLKPPVIIPPIDHSFK 163
>At4g20060.1 68417.m02935 expressed protein ; expression supported by
MPSS
Length = 1134
Score = 29.5 bits (63), Expect = 1.7
Identities = 18/67 (26%), Positives = 33/67 (49%), Gaps = 3/67 (4%)
Frame = -1
Query: 498 VQDLIKQL--IDQNKVI-LDVLFRNLAKIRLHHINDLQQELKYHGRVHILLCDCCQPNIC 328
V+DL+++L +D N L++L + H+ Q L+ G+V +LL C C
Sbjct: 844 VEDLVRRLWKVDPNVCEKLNILVNTNESLNCFHLQSRNQVLRVCGKVKMLLSICRDALSC 903
Query: 327 TLDMEER 307
T ++ +
Sbjct: 904 TYGLQNQ 910
>At2g19790.1 68415.m02312 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 143
Score = 27.9 bits (59), Expect = 5.2
Identities = 19/114 (16%), Positives = 49/114 (42%), Gaps = 2/114 (1%)
Frame = +1
Query: 160 LFVYNHKGEVLISRVYR--DDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHIKRAN 333
+ + N +G+ +++ Y R A++ V AR + + + A+
Sbjct: 6 ILMVNKQGQTRLAQYYEWLTLEERRALEGEIVRKCLARNDQQCSFVEHRNYKIVYRRYAS 65
Query: 334 IWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEIL 495
++ + N + EF+ +++ M +FG + E +I + + +L+E++
Sbjct: 66 LFFMVGVDDDENELAILEFIHLLVETMDKHFGNVCELDIMFHLEKAHFMLEEMV 119
>At5g01270.1 68418.m00036 double-stranded RNA-binding domain
(DsRBD)-containing protein contains Pfam profile
PF00035: Double-stranded RNA binding motif
Length = 771
Score = 27.5 bits (58), Expect = 6.9
Identities = 9/32 (28%), Positives = 17/32 (53%)
Frame = -1
Query: 255 HIHSECVHSITTNIISVDSRDQHLAFMVVNEQ 160
H+H+ C H + T ++ + + HL M E+
Sbjct: 81 HLHAVCFHELKTAVVMLGDEEIHLVAMPSKEK 112
>At2g42720.1 68415.m05291 F-box family protein contains F-box domain
Pfam:PF00646
Length = 443
Score = 27.5 bits (58), Expect = 6.9
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = -1
Query: 549 SDECLQDPSIRILRVTKVQDLIKQLIDQNKVILDVLFRNLAKIR 418
S CL +++L + Q K+L ++ + F L KIR
Sbjct: 370 SSSCLSSSRVKVLEIWSYQGTSKELKQMGHFLMKLQFLELVKIR 413
>At5g40480.1 68418.m04909 expressed protein ; expression supported by
MPSS
Length = 1919
Score = 27.1 bits (57), Expect = 9.1
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = +1
Query: 175 HKGEVLISRVYRDDIGRNAVDAFRVNVIHARQ 270
+KG + IS + RDD G ++VNV+ + Q
Sbjct: 1757 NKGRLSISLIKRDDFGIAGHAQYKVNVLRSEQ 1788
>At1g65440.1 68414.m07424 glycine-rich protein
Length = 1647
Score = 27.1 bits (57), Expect = 9.1
Identities = 15/52 (28%), Positives = 27/52 (51%)
Frame = -1
Query: 477 LIDQNKVILDVLFRNLAKIRLHHINDLQQELKYHGRVHILLCDCCQPNICTL 322
++D + +LDVL+ R ++ND Q++ RV + D QP++ L
Sbjct: 734 MLDSSGEVLDVLYAGSLTSRSQNVNDQQRKKSDQDRVLKFMMD-HQPHVVAL 784
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,600,679
Number of Sequences: 28952
Number of extensions: 260379
Number of successful extensions: 595
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 577
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 593
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1141585696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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