BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11j03r (551 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1682.14 |rpl1902|rpl19-2|60S ribosomal protein L19B|Schizosa... 188 4e-49 SPBC56F2.02 |rpl1901|rpl19-1|60S ribosomal protein L19|Schizosac... 188 4e-49 SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 30 0.20 SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr 2... 26 3.2 SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Sc... 26 4.2 SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces ... 25 5.6 SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 25 7.4 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 25 9.8 SPAC13G7.12c |||choline kinase |Schizosaccharomyces pombe|chr 1|... 25 9.8 SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|... 25 9.8 SPAC1D4.09c |||DUF602 family protein|Schizosaccharomyces pombe|c... 25 9.8 >SPCC1682.14 |rpl1902|rpl19-2|60S ribosomal protein L19B|Schizosaccharomyces pombe|chr 3|||Manual Length = 193 Score = 188 bits (459), Expect = 4e-49 Identities = 86/158 (54%), Positives = 114/158 (72%) Frame = -2 Query: 550 VMRCVKKKVWLDPNEINEIANTNSXQNIRKMIKDGLVIKKPVAVHSRARVRKNTEARRKG 371 V++C K+KVW+DPNEI+EI+N NS QNIRK++KDGLVI+KP +HSR R+RK A+R G Sbjct: 14 VLKCGKRKVWMDPNEISEISNANSRQNIRKLVKDGLVIRKPNLMHSRFRIRKTHAAKRLG 73 Query: 370 RHCGFGKRRGTANARMPQKELWXXXXXXXXXXXXXXRTAKKIDRHLYHSLYMKAKGNVFK 191 RH G+GKR+GTA ARMP +W R + KID+HLYH+LY++AKGN FK Sbjct: 74 RHTGYGKRKGTAEARMPSTVVWMRRQRVLRRLLRKYRESGKIDKHLYHTLYLEAKGNTFK 133 Query: 190 NKRVLMEYIHRKKAEKARTKMLSDQAEAXRNKVKEASQ 77 +KR L+E+I R KAE RTK++ +Q +A R + K A Q Sbjct: 134 HKRALIEHIQRAKAEANRTKLIQEQQDARRARAKAARQ 171 >SPBC56F2.02 |rpl1901|rpl19-1|60S ribosomal protein L19|Schizosaccharomyces pombe|chr 2|||Manual Length = 193 Score = 188 bits (459), Expect = 4e-49 Identities = 86/158 (54%), Positives = 114/158 (72%) Frame = -2 Query: 550 VMRCVKKKVWLDPNEINEIANTNSXQNIRKMIKDGLVIKKPVAVHSRARVRKNTEARRKG 371 V++C K+KVW+DPNEI+EI+N NS QN+RK+IKDGLVI+KP +HSR R+RK A+R G Sbjct: 14 VLKCGKRKVWMDPNEISEISNANSRQNVRKLIKDGLVIRKPNLMHSRFRIRKTHAAKRLG 73 Query: 370 RHCGFGKRRGTANARMPQKELWXXXXXXXXXXXXXXRTAKKIDRHLYHSLYMKAKGNVFK 191 RH G+GKR+GTA ARMP +W R + KID+HLYH+LY++AKGN FK Sbjct: 74 RHTGYGKRKGTAEARMPSAVVWMRRQRVLRRLLRKYRESGKIDKHLYHTLYLEAKGNTFK 133 Query: 190 NKRVLMEYIHRKKAEKARTKMLSDQAEAXRNKVKEASQ 77 +KR L+E+I R KAE RTK++ +Q +A R + K A Q Sbjct: 134 HKRALIEHIQRAKAEANRTKLIQEQQDARRARAKAARQ 171 >SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces pombe|chr 2|||Manual Length = 1309 Score = 30.3 bits (65), Expect = 0.20 Identities = 12/33 (36%), Positives = 22/33 (66%) Frame = +2 Query: 446 TILDHLTDVLXGVGVCDLIDFIXIQPHLLFNTS 544 +I +H++DV G+ V LI+ + I+P +LF + Sbjct: 351 SICEHVSDVSRGIAVETLIELMIIRPKILFKAN 383 >SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 497 Score = 26.2 bits (55), Expect = 3.2 Identities = 10/17 (58%), Positives = 11/17 (64%) Frame = +1 Query: 310 VPSVAYAHWQYLFSYQS 360 +PSV YA WQ L Y S Sbjct: 255 IPSVCYAAWQLLAKYDS 271 >SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 611 Score = 25.8 bits (54), Expect = 4.2 Identities = 11/32 (34%), Positives = 17/32 (53%), Gaps = 1/32 (3%) Frame = +3 Query: 150 AFFLWMYSMSTRLFLNTLPFAFM-*SEWYRCL 242 AF LW+YS+ L+L + W++CL Sbjct: 337 AFVLWVYSLDKELWLQLDMLGVLNHGSWFKCL 368 >SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 1|||Manual Length = 444 Score = 25.4 bits (53), Expect = 5.6 Identities = 16/65 (24%), Positives = 31/65 (47%), Gaps = 11/65 (16%) Frame = -2 Query: 529 KVWLDPNEINEIANTNSXQNIRKMIKDGLVIKK-----------PVAVHSRARVRKNTEA 383 K WLDP ++++ T + +++++ I ++K+ P + A KN EA Sbjct: 289 KSWLDPVVVSKVKFTRNYRDLQQYINPDNILKEFGGPNPWRYTYPEPCQNEAEALKNVEA 348 Query: 382 RRKGR 368 R+ R Sbjct: 349 RKSLR 353 >SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 25.0 bits (52), Expect = 7.4 Identities = 11/39 (28%), Positives = 20/39 (51%) Frame = -2 Query: 475 QNIRKMIKDGLVIKKPVAVHSRARVRKNTEARRKGRHCG 359 +++R +KD + +PV+V S A ++T G G Sbjct: 141 ESLRSKLKDAMASTEPVSVDSAAAPSQSTNPEGNGGSVG 179 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 24.6 bits (51), Expect = 9.8 Identities = 8/33 (24%), Positives = 20/33 (60%) Frame = -2 Query: 487 TNSXQNIRKMIKDGLVIKKPVAVHSRARVRKNT 389 T S ++ +IK+ +KP ++++++KN+ Sbjct: 912 TESLNQVKNLIKNDTSNRKPFGAENQSKLKKNS 944 >SPAC13G7.12c |||choline kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 456 Score = 24.6 bits (51), Expect = 9.8 Identities = 10/28 (35%), Positives = 16/28 (57%) Frame = +2 Query: 443 ETILDHLTDVLXGVGVCDLIDFIXIQPH 526 +TI D + G +C+L +FI + PH Sbjct: 145 KTIRDPKLSIYVGRRLCELHNFILLHPH 172 >SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|chr 3|||Manual Length = 828 Score = 24.6 bits (51), Expect = 9.8 Identities = 16/37 (43%), Positives = 23/37 (62%) Frame = +2 Query: 110 LSLVAKHLRPGLLSLLPVDVLHEHTLVLEHITLRLHV 220 LSL K + + +LL + + H HTLV E +TL+L V Sbjct: 324 LSLPVKSVESSVQALLFMVLPHLHTLVNE-LTLKLFV 359 >SPAC1D4.09c |||DUF602 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 240 Score = 24.6 bits (51), Expect = 9.8 Identities = 9/22 (40%), Positives = 14/22 (63%) Frame = +1 Query: 346 FSYQSHSDDPFYVPLCFCGHGR 411 FS + +D+P Y P+ CG G+ Sbjct: 37 FSQCAITDEPLYPPIVSCGLGK 58 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,135,600 Number of Sequences: 5004 Number of extensions: 42191 Number of successful extensions: 126 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 123 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 124 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 229961028 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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