BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11i24f (630 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|... 260 1e-70 SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase... 259 2e-70 SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 27 3.0 SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 26 5.2 SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces pom... 26 5.2 SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces ... 25 6.8 SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 25 6.8 SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 25 6.8 SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit S... 25 6.8 >SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|Schizosaccharomyces pombe|chr 2|||Manual Length = 335 Score = 260 bits (637), Expect = 1e-70 Identities = 125/170 (73%), Positives = 144/170 (84%), Gaps = 1/170 (0%) Frame = +1 Query: 94 KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 270 K+GINGFGRIGR+VLR +I G QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE + Sbjct: 5 KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64 Query: 271 GFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAP 450 G LV++G+ I V +ERDP I W +GAEYV+ESTGVFTT + ASAHL+GGAK+VIISAP Sbjct: 65 GKLVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAP 124 Query: 451 SADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGL 600 S DAPMFVVGVNLE ++PS KVISNASCTTNCLAPLAKVI+D F I EGL Sbjct: 125 SKDAPMFVVGVNLEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGL 174 Score = 25.0 bits (52), Expect = 9.0 Identities = 13/27 (48%), Positives = 16/27 (59%) Frame = +2 Query: 548 LPHLQRLFMITLKLLRXLMTTVHATTA 628 L L ++ T + LMTTVHATTA Sbjct: 157 LAPLAKVINDTFGIEEGLMTTVHATTA 183 >SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase Tdh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 336 Score = 259 bits (635), Expect = 2e-70 Identities = 124/170 (72%), Positives = 144/170 (84%), Gaps = 1/170 (0%) Frame = +1 Query: 94 KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 270 K+GINGFGRIGR+VLR A + K QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++D Sbjct: 5 KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64 Query: 271 GFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAP 450 G LV++GN I V +ERDP I W +GA+YV+ESTGVFTT + ASAHL+GGAK+VIISAP Sbjct: 65 GKLVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAP 124 Query: 451 SADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGL 600 S DAPM+VVGVN E ++PS KVISNASCTTNCLAPLAKVI+D F I EGL Sbjct: 125 SKDAPMYVVGVNEEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGL 174 Score = 25.0 bits (52), Expect = 9.0 Identities = 13/27 (48%), Positives = 16/27 (59%) Frame = +2 Query: 548 LPHLQRLFMITLKLLRXLMTTVHATTA 628 L L ++ T + LMTTVHATTA Sbjct: 157 LAPLAKVINDTFGIEEGLMTTVHATTA 183 >SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1583 Score = 26.6 bits (56), Expect = 3.0 Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 3/55 (5%) Frame = -1 Query: 300 NFVTVNNKESILNLNTALKTAMGGIILEKINHIVK---TDERVIYSDHLSSLFNR 145 +F + N+ESIL+L L + + E I++IV+ +D R HLS L + Sbjct: 338 SFKKLQNEESILHLLNILHSIFEYTVPEAIDNIVQSKTSDARTSEIQHLSVLLQK 392 >SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1958 Score = 25.8 bits (54), Expect = 5.2 Identities = 8/18 (44%), Positives = 13/18 (72%) Frame = +1 Query: 562 KVIHDNFEIVEGLDDYCS 615 K+ + F +VEGL +YC+ Sbjct: 1032 KICENRFPVVEGLQEYCN 1049 >SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 25.8 bits (54), Expect = 5.2 Identities = 15/62 (24%), Positives = 29/62 (46%), Gaps = 1/62 (1%) Frame = -1 Query: 459 ISTGS*YNNFFSTSLQVSRCFICSGKDTSRLYNIFSPSFSPWNGLRVPF*E-NGNFVTVN 283 + +G +N +S + C +C G + ++ +P F +R+P NGN T+ Sbjct: 153 VCSGQGFNPKYSADKAIESCPVCGGSGFRVIEHMIAPGFR--QQMRMPCNACNGNGRTIK 210 Query: 282 NK 277 +K Sbjct: 211 HK 212 >SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces pombe|chr 1|||Manual Length = 1888 Score = 25.4 bits (53), Expect = 6.8 Identities = 18/65 (27%), Positives = 33/65 (50%), Gaps = 1/65 (1%) Frame = -1 Query: 303 GNFVTVNNKESILNLNTALKTAMGGIILEKINHIVKTDERVIYSDHL-SSLFNRSTEHQT 127 GN +VN+K+ +N+N KTA ++H+ E ++ L SSLF+ + ++ Sbjct: 519 GNIFSVNSKKHSVNINA--KTAAN----NGLSHLQNFSEELLKKRKLFSSLFSNNVSYKK 572 Query: 126 ANAAK 112 + K Sbjct: 573 SKKLK 577 >SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosaccharomyces pombe|chr 2|||Manual Length = 502 Score = 25.4 bits (53), Expect = 6.8 Identities = 14/37 (37%), Positives = 19/37 (51%) Frame = +1 Query: 109 GFGRIGRLVLRASIEKGAQVVAINDPFIGLDYMVYLF 219 GF RI +R EKG QVV D I + + +L+ Sbjct: 298 GFVRIESQKIRQHAEKGEQVVFTGDRVIQTNVVPFLY 334 >SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 25.4 bits (53), Expect = 6.8 Identities = 11/16 (68%), Positives = 13/16 (81%) Frame = +3 Query: 18 LHLVLSVRGGGQLFNK 65 LHLVL +RGG Q+F K Sbjct: 67 LHLVLRLRGGMQIFVK 82 Score = 25.4 bits (53), Expect = 6.8 Identities = 11/16 (68%), Positives = 13/16 (81%) Frame = +3 Query: 18 LHLVLSVRGGGQLFNK 65 LHLVL +RGG Q+F K Sbjct: 143 LHLVLRLRGGMQIFVK 158 Score = 25.4 bits (53), Expect = 6.8 Identities = 11/16 (68%), Positives = 13/16 (81%) Frame = +3 Query: 18 LHLVLSVRGGGQLFNK 65 LHLVL +RGG Q+F K Sbjct: 219 LHLVLRLRGGMQIFVK 234 Score = 25.4 bits (53), Expect = 6.8 Identities = 11/16 (68%), Positives = 13/16 (81%) Frame = +3 Query: 18 LHLVLSVRGGGQLFNK 65 LHLVL +RGG Q+F K Sbjct: 295 LHLVLRLRGGMQIFVK 310 >SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit Ssb1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 609 Score = 25.4 bits (53), Expect = 6.8 Identities = 16/77 (20%), Positives = 38/77 (49%), Gaps = 6/77 (7%) Frame = +1 Query: 76 LADNMSKIGINGFGRIGRLVLRASIEKGAQVVAINDPFIGLD------YMVYLFKYDSTH 237 + D+ ++ +N F +G+L++ + ++ + ND ++ YM Y+F+ + Sbjct: 515 VGDHTGQLWLNVFDDVGKLIMHKTADE-LNDLQENDENAFMNCMAEACYMPYIFQCRAKQ 573 Query: 238 GRFKGSVEVQDGFLVVN 288 FKG + V+ + +N Sbjct: 574 DNFKGEMRVRYTVMSIN 590 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,698,947 Number of Sequences: 5004 Number of extensions: 57530 Number of successful extensions: 197 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 184 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 195 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 279695522 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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