SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV11i24f
         (630 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|...   260   1e-70
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase...   259   2e-70
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy...    27   3.0  
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc...    26   5.2  
SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces pom...    26   5.2  
SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces ...    25   6.8  
SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa...    25   6.8  
SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M...    25   6.8  
SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit S...    25   6.8  

>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
           Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 335

 Score =  260 bits (637), Expect = 1e-70
 Identities = 125/170 (73%), Positives = 144/170 (84%), Gaps = 1/170 (0%)
 Frame = +1

Query: 94  KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 270
           K+GINGFGRIGR+VLR +I  G  QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE + 
Sbjct: 5   KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64

Query: 271 GFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAP 450
           G LV++G+ I V +ERDP  I W  +GAEYV+ESTGVFTT + ASAHL+GGAK+VIISAP
Sbjct: 65  GKLVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAP 124

Query: 451 SADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGL 600
           S DAPMFVVGVNLE ++PS KVISNASCTTNCLAPLAKVI+D F I EGL
Sbjct: 125 SKDAPMFVVGVNLEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGL 174



 Score = 25.0 bits (52), Expect = 9.0
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = +2

Query: 548 LPHLQRLFMITLKLLRXLMTTVHATTA 628
           L  L ++   T  +   LMTTVHATTA
Sbjct: 157 LAPLAKVINDTFGIEEGLMTTVHATTA 183


>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
           Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 336

 Score =  259 bits (635), Expect = 2e-70
 Identities = 124/170 (72%), Positives = 144/170 (84%), Gaps = 1/170 (0%)
 Frame = +1

Query: 94  KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 270
           K+GINGFGRIGR+VLR A + K  QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++D
Sbjct: 5   KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64

Query: 271 GFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAP 450
           G LV++GN I V +ERDP  I W  +GA+YV+ESTGVFTT + ASAHL+GGAK+VIISAP
Sbjct: 65  GKLVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAP 124

Query: 451 SADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGL 600
           S DAPM+VVGVN E ++PS KVISNASCTTNCLAPLAKVI+D F I EGL
Sbjct: 125 SKDAPMYVVGVNEEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGL 174



 Score = 25.0 bits (52), Expect = 9.0
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = +2

Query: 548 LPHLQRLFMITLKLLRXLMTTVHATTA 628
           L  L ++   T  +   LMTTVHATTA
Sbjct: 157 LAPLAKVINDTFGIEEGLMTTVHATTA 183


>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1583

 Score = 26.6 bits (56), Expect = 3.0
 Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 3/55 (5%)
 Frame = -1

Query: 300 NFVTVNNKESILNLNTALKTAMGGIILEKINHIVK---TDERVIYSDHLSSLFNR 145
           +F  + N+ESIL+L   L +     + E I++IV+   +D R     HLS L  +
Sbjct: 338 SFKKLQNEESILHLLNILHSIFEYTVPEAIDNIVQSKTSDARTSEIQHLSVLLQK 392


>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
            Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1958

 Score = 25.8 bits (54), Expect = 5.2
 Identities = 8/18 (44%), Positives = 13/18 (72%)
 Frame = +1

Query: 562  KVIHDNFEIVEGLDDYCS 615
            K+  + F +VEGL +YC+
Sbjct: 1032 KICENRFPVVEGLQEYCN 1049


>SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 381

 Score = 25.8 bits (54), Expect = 5.2
 Identities = 15/62 (24%), Positives = 29/62 (46%), Gaps = 1/62 (1%)
 Frame = -1

Query: 459 ISTGS*YNNFFSTSLQVSRCFICSGKDTSRLYNIFSPSFSPWNGLRVPF*E-NGNFVTVN 283
           + +G  +N  +S    +  C +C G     + ++ +P F     +R+P    NGN  T+ 
Sbjct: 153 VCSGQGFNPKYSADKAIESCPVCGGSGFRVIEHMIAPGFR--QQMRMPCNACNGNGRTIK 210

Query: 282 NK 277
           +K
Sbjct: 211 HK 212


>SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1888

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 18/65 (27%), Positives = 33/65 (50%), Gaps = 1/65 (1%)
 Frame = -1

Query: 303 GNFVTVNNKESILNLNTALKTAMGGIILEKINHIVKTDERVIYSDHL-SSLFNRSTEHQT 127
           GN  +VN+K+  +N+N   KTA        ++H+    E ++    L SSLF+ +  ++ 
Sbjct: 519 GNIFSVNSKKHSVNINA--KTAAN----NGLSHLQNFSEELLKKRKLFSSLFSNNVSYKK 572

Query: 126 ANAAK 112
           +   K
Sbjct: 573 SKKLK 577


>SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase
           Ubp10|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 502

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 14/37 (37%), Positives = 19/37 (51%)
 Frame = +1

Query: 109 GFGRIGRLVLRASIEKGAQVVAINDPFIGLDYMVYLF 219
           GF RI    +R   EKG QVV   D  I  + + +L+
Sbjct: 298 GFVRIESQKIRQHAEKGEQVVFTGDRVIQTNVVPFLY 334


>SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 382

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +3

Query: 18  LHLVLSVRGGGQLFNK 65
           LHLVL +RGG Q+F K
Sbjct: 67  LHLVLRLRGGMQIFVK 82



 Score = 25.4 bits (53), Expect = 6.8
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +3

Query: 18  LHLVLSVRGGGQLFNK 65
           LHLVL +RGG Q+F K
Sbjct: 143 LHLVLRLRGGMQIFVK 158



 Score = 25.4 bits (53), Expect = 6.8
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +3

Query: 18  LHLVLSVRGGGQLFNK 65
           LHLVL +RGG Q+F K
Sbjct: 219 LHLVLRLRGGMQIFVK 234



 Score = 25.4 bits (53), Expect = 6.8
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +3

Query: 18  LHLVLSVRGGGQLFNK 65
           LHLVL +RGG Q+F K
Sbjct: 295 LHLVLRLRGGMQIFVK 310


>SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit Ssb1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 609

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 16/77 (20%), Positives = 38/77 (49%), Gaps = 6/77 (7%)
 Frame = +1

Query: 76  LADNMSKIGINGFGRIGRLVLRASIEKGAQVVAINDPFIGLD------YMVYLFKYDSTH 237
           + D+  ++ +N F  +G+L++  + ++    +  ND    ++      YM Y+F+  +  
Sbjct: 515 VGDHTGQLWLNVFDDVGKLIMHKTADE-LNDLQENDENAFMNCMAEACYMPYIFQCRAKQ 573

Query: 238 GRFKGSVEVQDGFLVVN 288
             FKG + V+   + +N
Sbjct: 574 DNFKGEMRVRYTVMSIN 590


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,698,947
Number of Sequences: 5004
Number of extensions: 57530
Number of successful extensions: 197
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -