Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11i24f
(630 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenas... 245 2e-65
At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenas... 244 4e-65
At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenas... 228 2e-60
At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenas... 227 4e-60
At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenas... 155 2e-38
At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenas... 150 7e-37
At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenas... 147 5e-36
At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identi... 29 2.5
At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identi... 29 2.5
At2g44030.1 68415.m05474 kelch repeat-containing F-box family pr... 29 2.5
At4g01330.1 68417.m00173 protein kinase family protein contains ... 29 3.4
At3g03910.1 68416.m00405 glutamate dehydrogenase, putative simil... 29 3.4
At1g21440.1 68414.m02681 mutase family protein similar to carbox... 28 4.4
At3g63330.1 68416.m07125 protein kinase family protein contains ... 27 7.8
>At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 422
Score = 245 bits (600), Expect = 2e-65
Identities = 115/172 (66%), Positives = 136/172 (79%), Gaps = 2/172 (1%)
Frame = +1
Query: 91 SKIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV- 264
+K+GINGFGRIGRLVLR A+ +VVA+NDPFI YM Y+ KYDSTHG FKGS+ V
Sbjct: 87 TKVGINGFGRIGRLVLRIATSRDDIEVVAVNDPFIDAKYMAYMLKYDSTHGNFKGSINVI 146
Query: 265 QDGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIIS 444
D L +NG K+ V S+RDP IPW GA+YVVES+GVFTT KA++HL+GGAKKVIIS
Sbjct: 147 DDSTLEINGKKVNVVSKRDPSEIPWADLGADYVVESSGVFTTLSKAASHLKGGAKKVIIS 206
Query: 445 APSADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGL 600
APSADAPMFVVGVN Y P+ ++SNASCTTNCLAPLAKV+H+ F I+EGL
Sbjct: 207 APSADAPMFVVGVNEHTYQPNMDIVSNASCTTNCLAPLAKVVHEEFGILEGL 258
>At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 420
Score = 244 bits (597), Expect = 4e-65
Identities = 114/172 (66%), Positives = 138/172 (80%), Gaps = 2/172 (1%)
Frame = +1
Query: 91 SKIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV- 264
+K+GINGFGRIGRLVLR A+ +VVA+NDPFI YM Y+FKYDSTHG +KG++ V
Sbjct: 85 TKVGINGFGRIGRLVLRIATFRDDIEVVAVNDPFIDAKYMAYMFKYDSTHGNYKGTINVI 144
Query: 265 QDGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIIS 444
D L +NG ++ V S+RDP IPW GAEYVVES+GVFTT +AS+HL+GGAKKVIIS
Sbjct: 145 DDSTLEINGKQVKVVSKRDPAEIPWADLGAEYVVESSGVFTTVGQASSHLKGGAKKVIIS 204
Query: 445 APSADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGL 600
APSADAPMFVVGVN + Y P+ ++SNASCTTNCLAPLAKV+H+ F I+EGL
Sbjct: 205 APSADAPMFVVGVNEKTYLPNMDIVSNASCTTNCLAPLAKVVHEEFGILEGL 256
>At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative very
strong similarity to SP|P25858 Glyceraldehyde
3-phosphate dehydrogenase, cytosolic (EC 1.2.1.12)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 338
Score = 228 bits (558), Expect = 2e-60
Identities = 110/178 (61%), Positives = 139/178 (78%), Gaps = 3/178 (1%)
Frame = +1
Query: 76 LADNMSKIGINGFGRIGRLVLRASIEKG-AQVVAINDPFIGLDYMVYLFKYDSTHGRFKG 252
+AD +IGINGFGRIGRLV R +++ ++VA+NDPFI +YM Y+FKYDS HG++K
Sbjct: 1 MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWKH 60
Query: 253 -SVEVQDGFLVVNGNK-IAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGA 426
++V+D ++ G K + VF R+P+ IPWG+AGA++VVESTGVFT DKA+AHL+GGA
Sbjct: 61 HELKVKDDKTLLFGEKPVTVFGIRNPEDIPWGEAGADFVVESTGVFTDKDKAAAHLKGGA 120
Query: 427 KKVIISAPSADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGL 600
KKV+ISAPS DAPMFVVGVN Y ++SNASCTTNCLAPLAKVI+D F IVEGL
Sbjct: 121 KKVVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVINDRFGIVEGL 178
>At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenase,
cytosolic (GAPC) / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase identical to
SP|P25858 Glyceraldehyde 3-phosphate dehydrogenase,
cytosolic (EC 1.2.1.12) {Arabidopsis thaliana}
Length = 338
Score = 227 bits (556), Expect = 4e-60
Identities = 109/178 (61%), Positives = 138/178 (77%), Gaps = 3/178 (1%)
Frame = +1
Query: 76 LADNMSKIGINGFGRIGRLVLRASIEKG-AQVVAINDPFIGLDYMVYLFKYDSTHGRFK- 249
+AD +IGINGFGRIGRLV R +++ ++VA+NDPFI +YM Y+FKYDS HG++K
Sbjct: 1 MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWKH 60
Query: 250 GSVEVQDGFLVVNGNK-IAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGA 426
++++D ++ G K + VF R+P+ IPW +AGA+YVVESTGVFT DKA+AHL+GGA
Sbjct: 61 NELKIKDEKTLLFGEKPVTVFGIRNPEDIPWAEAGADYVVESTGVFTDKDKAAAHLKGGA 120
Query: 427 KKVIISAPSADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGL 600
KKV+ISAPS DAPMFVVGVN Y ++SNASCTTNCLAPLAKVI+D F IVEGL
Sbjct: 121 KKVVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVINDRFGIVEGL 178
>At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenase,
chloroplast, putative / NADP-dependent
glyceraldehydephosphate dehydrogenase, putative similar
to SP|P25856 Glyceraldehyde 3-phosphate dehydrogenase A,
chloroplast precursor (EC 1.2.1.13) (NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 399
Score = 155 bits (377), Expect = 2e-38
Identities = 84/173 (48%), Positives = 105/173 (60%), Gaps = 5/173 (2%)
Frame = +1
Query: 94 KIGINGFGRIGRLVLRA---SIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV 264
K+ INGFGRIGR LR + VV IND G+ +L KYDST G F V+
Sbjct: 66 KVAINGFGRIGRNFLRCWHGRKDSPLDVVVINDTG-GVKQASHLLKYDSTLGIFDADVKP 124
Query: 265 Q-DGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVII 441
D L V+G I + S+R+P +PWG+ G + V+E TGVF D A HL+ GAKKV+I
Sbjct: 125 SGDSALSVDGKIIKIVSDRNPSNLPWGELGIDLVIEGTGVFVDRDGAGKHLQAGAKKVLI 184
Query: 442 SAP-SADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEG 597
+AP D P +VVGVN E Y +ISNASCTTNCLAP KV+ F I++G
Sbjct: 185 TAPGKGDIPTYVVGVNAELYSHEDTIISNASCTTNCLAPFVKVLDQKFGIIKG 237
>At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenase
A, chloroplast (GAPA) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A
identical to SP|P25856 Glyceraldehyde 3-phosphate
dehydrogenase A, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit A) {Arabidopsis thaliana}
Length = 396
Score = 150 bits (364), Expect = 7e-37
Identities = 81/173 (46%), Positives = 105/173 (60%), Gaps = 5/173 (2%)
Frame = +1
Query: 94 KIGINGFGRIGRLVLRA---SIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV 264
K+ INGFGRIGR LR + ++AIND G+ +L KYDST G F V+
Sbjct: 63 KVAINGFGRIGRNFLRCWHGRKDSPLDIIAINDTG-GVKQASHLLKYDSTLGIFDADVKP 121
Query: 265 Q-DGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVII 441
+ + V+G I V S R+P +PW + G + V+E TGVF + A H+E GAKKVII
Sbjct: 122 SGETAISVDGKIIQVVSNRNPSLLPWKELGIDIVIEGTGVFVDREGAGKHIEAGAKKVII 181
Query: 442 SAPS-ADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEG 597
+AP D P +VVGVN +AY +ISNASCTTNCLAP KV+ F I++G
Sbjct: 182 TAPGKGDIPTYVVGVNADAYSHDEPIISNASCTTNCLAPFVKVLDQKFGIIKG 234
>At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenase
B, chloroplast (GAPB) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit B
identical to SP|P25857 Glyceraldehyde 3-phosphate
dehydrogenase B, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit B) {Arabidopsis thaliana}
Length = 447
Score = 147 bits (357), Expect = 5e-36
Identities = 82/175 (46%), Positives = 107/175 (61%), Gaps = 7/175 (4%)
Frame = +1
Query: 94 KIGINGFGRIGRLVLRA---SIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV 264
K+ INGFGRIGR LR + +VV +ND G+ +L KYDS G FK V++
Sbjct: 83 KVAINGFGRIGRNFLRCWHGRKDSPLEVVVLNDSG-GVKNASHLLKYDSMLGTFKAEVKI 141
Query: 265 QDGFLV-VNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVII 441
D + V+G I V S RDP +PW + G + V+E TGVF A H++ GA KVII
Sbjct: 142 VDNETISVDGKLIKVVSNRDPLKLPWAELGIDIVIEGTGVFVDGPGAGKHIQAGASKVII 201
Query: 442 SAPS--ADAPMFVVGVNLEAYDPSF-KVISNASCTTNCLAPLAKVIHDNFEIVEG 597
+AP+ AD P +V+GVN + Y +ISNASCTTNCLAP AKV+ + F IV+G
Sbjct: 202 TAPAKGADIPTYVMGVNEQDYGHDVANIISNASCTTNCLAPFAKVLDEEFGIVKG 256
>At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identical
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 29.1 bits (62), Expect = 2.5
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +1
Query: 103 INGFGRIGRLVLRASIEKGAQVVAIND 183
I GFG +G + EKG ++VA++D
Sbjct: 211 IQGFGNVGSWAAKLISEKGGKIVAVSD 237
>At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identical
to glutamate dehydrogenase 2 (GDH 2) [Arabidopsis
thaliana] SWISS-PROT:Q38946
Length = 411
Score = 29.1 bits (62), Expect = 2.5
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +1
Query: 103 INGFGRIGRLVLRASIEKGAQVVAIND 183
I GFG +G + EKG +VVA++D
Sbjct: 211 IQGFGNVGTWAAKLIHEKGGKVVAVSD 237
>At2g44030.1 68415.m05474 kelch repeat-containing F-box family
protein low similarity to SKP1 interacting partner 6
[Arabidopsis thaliana] GI:10716957; contains Pfam
profiles PF01344: Kelch motif, PF00646: F-box domain
Length = 380
Score = 29.1 bits (62), Expect = 2.5
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = +2
Query: 128 VWCSVLLLKRELKWSL*MTLSSVLTI 205
+WC+++LL+REL W + +V T+
Sbjct: 331 IWCALILLERELVWGVIEWSENVFTL 356
>At4g01330.1 68417.m00173 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; contains
serine/threonine protein kinase domain,
INTERPRO:IPR002290
Length = 329
Score = 28.7 bits (61), Expect = 3.4
Identities = 14/32 (43%), Positives = 22/32 (68%)
Frame = -1
Query: 204 IVKTDERVIYSDHLSSLFNRSTEHQTANAAKS 109
I KT+ RV++SD +SS +R T +TA+ + S
Sbjct: 103 IGKTEHRVVFSDRVSSGESRGTVSETASYSGS 134
>At3g03910.1 68416.m00405 glutamate dehydrogenase, putative similar
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 28.7 bits (61), Expect = 3.4
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +1
Query: 94 KIGINGFGRIGRLVLRASIEKGAQVVAIND 183
+ I GFG +G + +KG ++VA++D
Sbjct: 208 RFAIQGFGNVGSWAAKLISDKGGKIVAVSD 237
>At1g21440.1 68414.m02681 mutase family protein similar to
carboxyvinyl-carboxyphosphonate phosphorylmutase
GB:O49290 from [Arabidopsis thaliana]; similar to
carboxyphosphonoenolpyruvate mutase (GI:47149)
[Streptomyces hygroscopicus]; contains Prosite PS00161:
Isocitrate lyase signature
Length = 336
Score = 28.3 bits (60), Expect = 4.4
Identities = 13/52 (25%), Positives = 24/52 (46%)
Frame = +1
Query: 343 KAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSADAPMFVVGVNLEAY 498
+ A + TG+ D+A+ ++E GA + AP D + +G + Y
Sbjct: 191 RTDARALSAKTGLSDAIDRANLYMEAGADASFVEAPRDDDELKEIGRRTKGY 242
>At3g63330.1 68416.m07125 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 376
Score = 27.5 bits (58), Expect = 7.8
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +2
Query: 377 VSLPLQIKHLLTWREVLKKLLYQLPVLMPPCLL 475
V+ L +H+ W E K+L Y+L LM C+L
Sbjct: 262 VTRALLDQHIRGWSENFKELAYKLRSLMEMCIL 294
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,298,416
Number of Sequences: 28952
Number of extensions: 310583
Number of successful extensions: 987
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 882
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 970
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1285411824
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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