Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11i16r
(694 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P55035 Cluster: 26S proteasome non-ATPase regulatory su... 87 5e-16
UniRef50_P55034 Cluster: 26S proteasome non-ATPase regulatory su... 78 2e-13
UniRef50_UPI0000F1FF43 Cluster: PREDICTED: similar to phosphatid... 73 9e-12
UniRef50_O61742 Cluster: Proteasome regulatory particle, non-atp... 62 2e-08
UniRef50_O17453 Cluster: 26S proteasome non-ATPase regulatory su... 61 3e-08
UniRef50_UPI00005A2BB8 Cluster: PREDICTED: similar to 26S protea... 57 5e-07
UniRef50_Q553E0 Cluster: VWFA domain-containing protein; n=2; Di... 45 0.002
UniRef50_Q4QIH4 Cluster: Proteasome regulatory non-ATP-ase subun... 44 0.005
UniRef50_Q5CQJ0 Cluster: 26S proteasome regulatory subunit 5a wi... 41 0.025
UniRef50_Q7XGY3 Cluster: 26S proteasome regulatory subunit S5A, ... 38 0.18
UniRef50_P38886 Cluster: 26S proteasome regulatory subunit RPN10... 38 0.23
UniRef50_Q5KP07 Cluster: RPN10-like protein, putative; n=2; Filo... 38 0.31
UniRef50_UPI00006CA4A3 Cluster: C2 domain containing protein; n=... 36 0.71
UniRef50_A7EV70 Cluster: Putative uncharacterized protein; n=1; ... 35 1.6
UniRef50_UPI00015552D5 Cluster: PREDICTED: similar to transitin;... 35 2.2
UniRef50_Q0U378 Cluster: Predicted protein; n=1; Phaeosphaeria n... 33 5.0
UniRef50_Q15SN0 Cluster: Phosphoglycerate mutase 1 family; n=1; ... 33 8.8
UniRef50_Q54HN4 Cluster: Putative uncharacterized protein; n=1; ... 33 8.8
UniRef50_Q4U9U3 Cluster: Putative uncharacterized protein; n=3; ... 33 8.8
>UniRef50_P55035 Cluster: 26S proteasome non-ATPase regulatory
subunit 4; n=3; Diptera|Rep: 26S proteasome non-ATPase
regulatory subunit 4 - Drosophila melanogaster (Fruit
fly)
Length = 396
Score = 86.6 bits (205), Expect = 5e-16
Identities = 48/83 (57%), Positives = 62/83 (74%), Gaps = 13/83 (15%)
Frame = -3
Query: 353 DAMELSEEEQIALAMQMSMQ--------QEA--PQAEES---MDVSEEYAEVMNDPAFLQ 213
D ++EEEQIA AMQMSMQ Q+A P+ +E+ MDV E+Y+EV+ DPAFLQ
Sbjct: 298 DFANMTEEEQIAFAMQMSMQDAPDDSVTQQAKRPKTDEANAPMDVDEDYSEVIGDPAFLQ 357
Query: 212 SVLENLPGVDPQSEAIRNAMSTI 144
SVLENLPGVDPQSEA+R+A+ ++
Sbjct: 358 SVLENLPGVDPQSEAVRDAVGSL 380
Score = 63.7 bits (148), Expect = 4e-09
Identities = 40/70 (57%), Positives = 44/70 (62%), Gaps = 1/70 (1%)
Frame = -3
Query: 689 LNGKDTSTGGSHLVSVPAGGCVVLSEALITS-XXXXXXXXXXXXXXXXPFEFGVDPNVDP 513
LNGKD + GSHLVSVP G VLS+AL++S FEFGVDPN DP
Sbjct: 160 LNGKDGT--GSHLVSVPRGS--VLSDALLSSPIIQGEDGMGGAGLGGNVFEFGVDPNEDP 215
Query: 512 ELALALRVSM 483
ELALALRVSM
Sbjct: 216 ELALALRVSM 225
>UniRef50_P55034 Cluster: 26S proteasome non-ATPase regulatory
subunit 4; n=11; Eukaryota|Rep: 26S proteasome
non-ATPase regulatory subunit 4 - Arabidopsis thaliana
(Mouse-ear cress)
Length = 386
Score = 77.8 bits (183), Expect = 2e-13
Identities = 65/202 (32%), Positives = 94/202 (46%), Gaps = 29/202 (14%)
Frame = -3
Query: 662 GSHLVSVPAGGC----VVLSEALITSXXXXXXXXXXXXXXXXP---FEFGVDPNVDPELA 504
GSH+V VP+G V+LS + T F+FGVDPN+DPELA
Sbjct: 168 GSHIVHVPSGANALSDVLLSTPVFTGDEGASGYVSAAAAAAAAGGDFDFGVDPNIDPELA 227
Query: 503 LALRVSMXXXXXXXXXXXXXXXXXXXXXXXXXXXGS-------------------DTA-V 384
LALRVSM S D+A +
Sbjct: 228 LALRVSMEEERARQEAAAKKAADEAGQKDKDGDTASASQETVARTTDKNAEPMDEDSALL 287
Query: 383 ERAIAMSLGRDAM-ELSEEEQ-IALAMQMSMQQEAPQAEESMDVSEEYAEVMNDPAFLQS 210
++AIAMS+G M E ++E+Q +ALA+QMSM EES + + ++ + AF+ S
Sbjct: 288 DQAIAMSVGDVNMSEAADEDQDLALALQMSM-----SGEESSEATGAGNNLLGNQAFISS 342
Query: 209 VLENLPGVDPQSEAIRNAMSTI 144
VL +LPGVDP A++ ++++
Sbjct: 343 VLSSLPGVDPNDPAVKELLASL 364
>UniRef50_UPI0000F1FF43 Cluster: PREDICTED: similar to
phosphatidylinositol-4-phosphate 5-kinase, type 1 beta;
n=1; Danio rerio|Rep: PREDICTED: similar to
phosphatidylinositol-4-phosphate 5-kinase, type 1 beta -
Danio rerio
Length = 292
Score = 72.5 bits (170), Expect = 9e-12
Identities = 43/84 (51%), Positives = 52/84 (61%), Gaps = 14/84 (16%)
Frame = -3
Query: 353 DAMELSEEEQIALAMQMSMQQEAPQAEESMDVS--------------EEYAEVMNDPAFL 216
D ++E+EQIA A+QMSMQ E+MDV EE +VM DP FL
Sbjct: 191 DFSRMTEDEQIAYALQMSMQGGEFGGSEAMDVDTAAAAAESEAPKEDEEDYDVMQDPEFL 250
Query: 215 QSVLENLPGVDPQSEAIRNAMSTI 144
QSVLENLPGVDP +EAIRNAM ++
Sbjct: 251 QSVLENLPGVDPNNEAIRNAMGSL 274
>UniRef50_O61742 Cluster: Proteasome regulatory particle,
non-atpase-like protein 10; n=2; Caenorhabditis|Rep:
Proteasome regulatory particle, non-atpase-like protein
10 - Caenorhabditis elegans
Length = 346
Score = 61.7 bits (143), Expect = 2e-08
Identities = 54/189 (28%), Positives = 83/189 (43%), Gaps = 15/189 (7%)
Frame = -3
Query: 692 TLNGKDTSTGGSHLVSVPAGGCVVLSEALITSXX---XXXXXXXXXXXXXXPFEFGVDPN 522
TLNGK+ S GS L+ VP G L++AL+ S FG+D
Sbjct: 161 TLNGKEGS--GSSLIVVPQGSS--LTDALLQSSVCKNEDGQAAFGGGGNGMDNAFGMDVE 216
Query: 521 VDPELALALRVSMXXXXXXXXXXXXXXXXXXXXXXXXXXXGSDTAVERAIAMSLGRDAME 342
DP+LALALRVSM + AV M +G
Sbjct: 217 NDPDLALALRVSMEEERARQAAAAAANGGAADSGADAEVAAAAAAVPLE-EMDMG----A 271
Query: 341 LSEEEQIALAMQMSMQQEAPQAE-----ESMDV-------SEEYAEVMNDPAFLQSVLEN 198
++EE+Q+ A+++SMQ+ AP + E MDV + ++MN+P LQ ++++
Sbjct: 272 MTEEQQLEWALRLSMQENAPAEQPQVQHEQMDVDGAPAVGGDNLDDLMNNPELLQQIVDD 331
Query: 197 LPGVDPQSE 171
LP + + +
Sbjct: 332 LPAANAEKD 340
>UniRef50_O17453 Cluster: 26S proteasome non-ATPase regulatory
subunit 4; n=7; Schistosoma|Rep: 26S proteasome
non-ATPase regulatory subunit 4 - Schistosoma mansoni
(Blood fluke)
Length = 420
Score = 60.9 bits (141), Expect = 3e-08
Identities = 54/199 (27%), Positives = 87/199 (43%), Gaps = 16/199 (8%)
Frame = -3
Query: 692 TLNGKDTSTGGSHLVSVPAGGCVVLSEALITSXXXXXXXXXXXXXXXXPFEFGVDPNVDP 513
TLNGKD + GSHL+SV G VL + L+TS EFG+D DP
Sbjct: 158 TLNGKDGT--GSHLISVAPG--TVLHDTLMTSPVVAGEDGSGMAGAGLGLEFGLDGAEDP 213
Query: 512 ELALALRVSMXXXXXXXXXXXXXXXXXXXXXXXXXXXGSDTA----VERAIAMSLGR--- 354
+L ALRVSM GS T+ +++A+AMS+
Sbjct: 214 DLLYALRVSMEDQRMRQEHEVNGDGSNTSVVATSLPAGSGTSEEAMLQQALAMSMQMNNT 273
Query: 353 ---------DAMELSEEEQIALAMQMSMQQEAPQAEESMDVSEEYAEVMNDPAFLQSVLE 201
D +SEE+QIA A++MS+QQ + + + E + + +P+ + ++
Sbjct: 274 ESSSLPMDIDLAAMSEEDQIAYALRMSLQQMGEETTQPTTTTLESDKTIVEPSGVAMDID 333
Query: 200 NLPGVDPQSEAIRNAMSTI 144
P ++ + ++ T+
Sbjct: 334 QTPTKVTENPNLSSSSGTL 352
Score = 40.7 bits (91), Expect = 0.033
Identities = 17/33 (51%), Positives = 26/33 (78%)
Frame = -3
Query: 242 EVMNDPAFLQSVLENLPGVDPQSEAIRNAMSTI 144
+VM D FL+SVL++LPGVD Q+E +R A++ +
Sbjct: 366 DVMYDAEFLESVLQSLPGVDTQNEDVRKAINAL 398
>UniRef50_UPI00005A2BB8 Cluster: PREDICTED: similar to 26S
proteasome non-ATPase regulatory subunit 4 (26S
proteasome regulatory subunit S5A) (Rpn10)
(Multiubiquitin chain binding protein) (Antisecretory
factor-1) (AF) (ASF); n=1; Canis lupus familiaris|Rep:
PREDICTED: similar to 26S proteasome non-ATPase
regulatory subunit 4 (26S proteasome regulatory subunit
S5A) (Rpn10) (Multiubiquitin chain binding protein)
(Antisecretory factor-1) (AF) (ASF) - Canis familiaris
Length = 205
Score = 56.8 bits (131), Expect = 5e-07
Identities = 53/176 (30%), Positives = 71/176 (40%)
Frame = -3
Query: 692 TLNGKDTSTGGSHLVSVPAGGCVVLSEALITSXXXXXXXXXXXXXXXXPFEFGVDPNVDP 513
TL G D + GS LV+VP G L++ALI+S FEFGVDP+ DP
Sbjct: 53 TLKGNDGT--GSPLVAVPPGPS--LADALISSPVLGWQGGAMLGLGASGFEFGVDPSADP 108
Query: 512 ELALALRVSMXXXXXXXXXXXXXXXXXXXXXXXXXXXGSDTAVERAIAMSLGRDAMELSE 333
+LALALRV + A A + E +
Sbjct: 109 QLALALRVLWKSSGGGPQRAPRPEQMTEEE-------------QIACARQMSPQGAEFGQ 155
Query: 332 EEQIALAMQMSMQQEAPQAEESMDVSEEYAEVMNDPAFLQSVLENLPGVDPQSEAI 165
E + + P EE + + + DP FLQSV E+LPG D +EA+
Sbjct: 156 AECADIDASSATDTSEPAKEE------DARDALRDPEFLQSVPESLPGEDLDNEAV 205
>UniRef50_Q553E0 Cluster: VWFA domain-containing protein; n=2;
Dictyostelium discoideum|Rep: VWFA domain-containing
protein - Dictyostelium discoideum AX4
Length = 349
Score = 44.8 bits (101), Expect = 0.002
Identities = 43/180 (23%), Positives = 76/180 (42%), Gaps = 8/180 (4%)
Frame = -3
Query: 659 SHLVSVPAGGCVVLSEALITSXXXXXXXXXXXXXXXXPFEFGVDPNVDPELALALRVSMX 480
SHL++VP G + LS+ ++ S EF ++ + DP+LA+AL++S+
Sbjct: 167 SHLLTVPPGPHI-LSDIILQSPIVESGSGQFGS------EF-INADTDPDLAMALKLSLE 218
Query: 479 XXXXXXXXXXXXXXXXXXXXXXXXXXGSDTAVERAIAMSLGRDAMELSEEEQIALAM-QM 303
+ E + ++ D EL+E +++A +M
Sbjct: 219 EEKQRQERERKAREEANGGSTNSGTTTTTAPTESNMDVNF-EDDPELAEALALSMATDKM 277
Query: 302 SMQQ-------EAPQAEESMDVSEEYAEVMNDPAFLQSVLENLPGVDPQSEAIRNAMSTI 144
+Q + PQ ++ + +E D FL S L +LPGVDP I+NA+ +
Sbjct: 278 EVQSSTTNTDSQPPQQQQQPPTDDTSSEAFKDQDFLNSTLNSLPGVDP--NRIKNALENL 335
>UniRef50_Q4QIH4 Cluster: Proteasome regulatory non-ATP-ase subunit,
putative; n=7; Trypanosomatidae|Rep: Proteasome
regulatory non-ATP-ase subunit, putative - Leishmania
major
Length = 346
Score = 43.6 bits (98), Expect = 0.005
Identities = 32/97 (32%), Positives = 43/97 (44%), Gaps = 6/97 (6%)
Frame = -3
Query: 539 FGVDPNVDPELALALRVSMXXXXXXXXXXXXXXXXXXXXXXXXXXXGSDT-AVERAIAMS 363
FGVDPN+DPELA+ALR+SM + T A A A +
Sbjct: 206 FGVDPNMDPELAMALRLSMEDEMQRQAAAAAAAASSAAPESAPASGSAATPAAPAAPAAA 265
Query: 362 LGRDAMEL-----SEEEQIALAMQMSMQQEAPQAEES 267
D EL SEEE + A+ +S+Q A A ++
Sbjct: 266 PVVDEDELSYENMSEEEMMRRAIALSLQDTAQGASDT 302
>UniRef50_Q5CQJ0 Cluster: 26S proteasome regulatory subunit 5a with
a vWA domain and two ubiquitin interacting motifs; n=2;
Cryptosporidium|Rep: 26S proteasome regulatory subunit
5a with a vWA domain and two ubiquitin interacting
motifs - Cryptosporidium parvum Iowa II
Length = 383
Score = 41.1 bits (92), Expect = 0.025
Identities = 21/48 (43%), Positives = 25/48 (52%)
Frame = -3
Query: 626 VVLSEALITSXXXXXXXXXXXXXXXXPFEFGVDPNVDPELALALRVSM 483
+VL E TS FEFG+DPN DPEL +ALR+SM
Sbjct: 189 IVLGEG--TSSGVYADGLAGAAGETSGFEFGIDPNADPELYMALRMSM 234
>UniRef50_Q7XGY3 Cluster: 26S proteasome regulatory subunit S5A,
putative; n=6; Oryza sativa|Rep: 26S proteasome
regulatory subunit S5A, putative - Oryza sativa subsp.
japonica (Rice)
Length = 550
Score = 38.3 bits (85), Expect = 0.18
Identities = 23/63 (36%), Positives = 31/63 (49%), Gaps = 3/63 (4%)
Frame = -3
Query: 665 GGSHLVSVPAGG---CVVLSEALITSXXXXXXXXXXXXXXXXPFEFGVDPNVDPELALAL 495
G SH+V +P G V+ + +IT +E+ VDPNVDPE A AL
Sbjct: 164 GSSHIVHIPPGEDLRAVLANTPIITGDEGGGAAAGGASR----YEYNVDPNVDPEFAEAL 219
Query: 494 RVS 486
R+S
Sbjct: 220 RLS 222
>UniRef50_P38886 Cluster: 26S proteasome regulatory subunit RPN10;
n=12; Dikarya|Rep: 26S proteasome regulatory subunit
RPN10 - Saccharomyces cerevisiae (Baker's yeast)
Length = 268
Score = 37.9 bits (84), Expect = 0.23
Identities = 15/20 (75%), Positives = 20/20 (100%)
Frame = -3
Query: 542 EFGVDPNVDPELALALRVSM 483
+FGVDP++DPELA+ALR+SM
Sbjct: 217 DFGVDPSMDPELAMALRLSM 236
>UniRef50_Q5KP07 Cluster: RPN10-like protein, putative; n=2;
Filobasidiella neoformans|Rep: RPN10-like protein,
putative - Cryptococcus neoformans (Filobasidiella
neoformans)
Length = 371
Score = 37.5 bits (83), Expect = 0.31
Identities = 23/59 (38%), Positives = 34/59 (57%)
Frame = -3
Query: 659 SHLVSVPAGGCVVLSEALITSXXXXXXXXXXXXXXXXPFEFGVDPNVDPELALALRVSM 483
S+LVSVP+G LS+ + +S FE +DPN+DPELA+A+R+S+
Sbjct: 166 SNLVSVPSGERF-LSDVIASSPILFDGENPMAAAGG--FEGDIDPNMDPELAMAIRMSL 221
>UniRef50_UPI00006CA4A3 Cluster: C2 domain containing protein; n=1;
Tetrahymena thermophila SB210|Rep: C2 domain containing
protein - Tetrahymena thermophila SB210
Length = 1302
Score = 36.3 bits (80), Expect = 0.71
Identities = 17/62 (27%), Positives = 35/62 (56%)
Frame = -3
Query: 356 RDAMELSEEEQIALAMQMSMQQEAPQAEESMDVSEEYAEVMNDPAFLQSVLENLPGVDPQ 177
R+ + +EEQ L +Q ++QEA + + + ++ E++N +Q V+ +L +P+
Sbjct: 841 RELEKKLKEEQKRLELQAKLEQEAKEEKRRQEEEQQRQEMINQQQEVQKVISDLQQKEPK 900
Query: 176 SE 171
SE
Sbjct: 901 SE 902
>UniRef50_A7EV70 Cluster: Putative uncharacterized protein; n=1;
Sclerotinia sclerotiorum 1980|Rep: Putative
uncharacterized protein - Sclerotinia sclerotiorum 1980
Length = 1188
Score = 35.1 bits (77), Expect = 1.6
Identities = 21/52 (40%), Positives = 31/52 (59%), Gaps = 3/52 (5%)
Frame = -3
Query: 395 DTAVERAIAMSLGRDA---MELSEEEQIALAMQMSMQQEAPQAEESMDVSEE 249
D + RAIAMSL +D EL EE+ + A+++S+Q+ Q EE D E+
Sbjct: 450 DEDINRAIAMSLQQDQGQDDELDEEDHLKRAIELSLQKGHGQEEEGDDPFED 501
>UniRef50_UPI00015552D5 Cluster: PREDICTED: similar to transitin;
n=1; Ornithorhynchus anatinus|Rep: PREDICTED: similar to
transitin - Ornithorhynchus anatinus
Length = 1378
Score = 34.7 bits (76), Expect = 2.2
Identities = 24/63 (38%), Positives = 34/63 (53%), Gaps = 3/63 (4%)
Frame = -3
Query: 347 MELSEEEQIALAMQMSMQQEAPQAEES---MDVSEEYAEVMNDPAFLQSVLENLPGVDPQ 177
M+ EEE+ A + EA + +++ M V +E AE M A L+S+ ENL G DPQ
Sbjct: 804 MQAEEEEEEIPAETAGAEDEAGEEKKTKKEMQVEKEEAE-MRPAAELESLEENLRGTDPQ 862
Query: 176 SEA 168
A
Sbjct: 863 KTA 865
>UniRef50_Q0U378 Cluster: Predicted protein; n=1; Phaeosphaeria
nodorum|Rep: Predicted protein - Phaeosphaeria nodorum
(Septoria nodorum)
Length = 458
Score = 33.5 bits (73), Expect = 5.0
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = +3
Query: 573 CSITTYQRTCYQSLRKHNTASSWHRHKVTSPS 668
C+I T+Q TC +LR+ + RHK+T+ S
Sbjct: 15 CNIVTFQFTCQHTLRRRRSRCGGTRHKITANS 46
>UniRef50_Q15SN0 Cluster: Phosphoglycerate mutase 1 family; n=1;
Pseudoalteromonas atlantica T6c|Rep: Phosphoglycerate
mutase 1 family - Pseudoalteromonas atlantica (strain
T6c / BAA-1087)
Length = 227
Score = 32.7 bits (71), Expect = 8.8
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +3
Query: 579 ITTYQRTCYQSLRKHNTASSWHRHKVTSPS 668
I + R C+Q +R H+T S W+ H SP+
Sbjct: 66 ILRHNRQCHQYVRIHDTGSQWYEHFEASPA 95
>UniRef50_Q54HN4 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 197
Score = 32.7 bits (71), Expect = 8.8
Identities = 16/52 (30%), Positives = 26/52 (50%), Gaps = 1/52 (1%)
Frame = -3
Query: 335 EEEQIALAMQMSMQQEAPQAEESMDVSEEYA-EVMNDPAFLQSVLENLPGVD 183
+++Q Q QQ+ PQ E D + A +V+ ++S +E LPG D
Sbjct: 85 QQQQQQQQQQQQQQQQQPQPTEEWDAPSKMALQVIETSKVIESYIEKLPGFD 136
>UniRef50_Q4U9U3 Cluster: Putative uncharacterized protein; n=3;
Piroplasmida|Rep: Putative uncharacterized protein -
Theileria annulata
Length = 332
Score = 32.7 bits (71), Expect = 8.8
Identities = 12/20 (60%), Positives = 18/20 (90%)
Frame = -3
Query: 542 EFGVDPNVDPELALALRVSM 483
+FGVDP +DP+L +ALR+S+
Sbjct: 209 DFGVDPEMDPQLYMALRLSL 228
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 605,181,652
Number of Sequences: 1657284
Number of extensions: 10316596
Number of successful extensions: 29862
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 28638
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 29809
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 54545459628
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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