BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11i16f (576 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g38630.1 68417.m05467 26S proteasome regulatory subunit S5A (... 187 4e-48 At1g05055.1 68414.m00506 basic transcription factor 2, 44kD subu... 44 6e-05 >At4g38630.1 68417.m05467 26S proteasome regulatory subunit S5A (RPN10) identical to multiubiquitin chain binding protein (MBP1) SP:P55034, GI:1165206 Length = 386 Score = 187 bits (456), Expect = 4e-48 Identities = 92/169 (54%), Positives = 123/169 (72%), Gaps = 4/169 (2%) Frame = +3 Query: 72 MVLESTMICVDNSDYMRNGDFLPTRLQAQQDAVNLVCHSKTRSNPENNVGLLTLAN--VE 245 MVLE+TMIC+DNS++MRNGD+ P+RLQAQ +AVNL+C +KT+SNPEN VG+LT+A V Sbjct: 1 MVLEATMICIDNSEWMRNGDYSPSRLQAQTEAVNLLCGAKTQSNPENTVGILTMAGKGVR 60 Query: 246 VLATLTSDVGRIMSKLHRVQPNGDINLLTGIRIAHLALKHRQGKNHKMRIVVFVGSPVNT 425 VL T TSD+G+I++ +H + G+INL I+IA LALKHRQ KN + RI+VF GSP+ Sbjct: 61 VLTTPTSDLGKILACMHGLDVGGEINLTAAIQIAQLALKHRQNKNQRQRIIVFAGSPIKY 120 Query: 426 DEKELVKLAKRLKKEKVNCDVVSFGE-DSENNP-LLTTFVNTLNGKDTS 566 ++K L + KRLKK V+ D+V+FGE D E P L + +N D S Sbjct: 121 EKKALEIVGKRLKKNSVSLDIVNFGEDDDEEKPQKLEALLTAVNNNDGS 169 >At1g05055.1 68414.m00506 basic transcription factor 2, 44kD subunit-related contains weak similarity to Swiss-Prot:Q13888 TFIIH basal transcription factor complex p44 subunit (Basic transcription factor 2 44 kDa subunit, BTF2-p44, General transcription factor IIH polypeptide 2) [Homo sapiens] Length = 421 Score = 44.4 bits (100), Expect = 6e-05 Identities = 33/151 (21%), Positives = 66/151 (43%), Gaps = 5/151 (3%) Frame = +3 Query: 69 KMVLESTMICVDNSDYMRNGDFLPTRLQAQQDAVNLVCHSKTRSNPENNVGLLTLANVEV 248 K ++ I +D S DF P+R+ V NP + +GL+++ N V Sbjct: 78 KGLIRYLYIVIDFSRAAAEMDFRPSRMAIMAKHVEAFIREFFDQNPLSQIGLVSIKN-GV 136 Query: 249 LATLTSDVG-----RIMSKLHRVQPNGDINLLTGIRIAHLALKHRQGKNHKMRIVVFVGS 413 TLT D+G I + + +++ GD +L + + H L H+ +++ + Sbjct: 137 AHTLT-DLGGSPETHIKALMGKLEALGDSSLQNALELVHEHLNQVPSYGHR-EVLILYSA 194 Query: 414 PVNTDEKELVKLAKRLKKEKVNCDVVSFGED 506 D ++++ ++ KK K+ C V+ + Sbjct: 195 LCTCDPGDIMETIQKCKKSKLRCSVIGLSAE 225 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,084,743 Number of Sequences: 28952 Number of extensions: 235522 Number of successful extensions: 576 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 570 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 575 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1121903184 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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