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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV11i16f
         (576 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g38630.1 68417.m05467 26S proteasome regulatory subunit S5A (...   187   4e-48
At1g05055.1 68414.m00506 basic transcription factor 2, 44kD subu...    44   6e-05

>At4g38630.1 68417.m05467 26S proteasome regulatory subunit S5A
           (RPN10) identical to multiubiquitin chain binding
           protein (MBP1) SP:P55034, GI:1165206
          Length = 386

 Score =  187 bits (456), Expect = 4e-48
 Identities = 92/169 (54%), Positives = 123/169 (72%), Gaps = 4/169 (2%)
 Frame = +3

Query: 72  MVLESTMICVDNSDYMRNGDFLPTRLQAQQDAVNLVCHSKTRSNPENNVGLLTLAN--VE 245
           MVLE+TMIC+DNS++MRNGD+ P+RLQAQ +AVNL+C +KT+SNPEN VG+LT+A   V 
Sbjct: 1   MVLEATMICIDNSEWMRNGDYSPSRLQAQTEAVNLLCGAKTQSNPENTVGILTMAGKGVR 60

Query: 246 VLATLTSDVGRIMSKLHRVQPNGDINLLTGIRIAHLALKHRQGKNHKMRIVVFVGSPVNT 425
           VL T TSD+G+I++ +H +   G+INL   I+IA LALKHRQ KN + RI+VF GSP+  
Sbjct: 61  VLTTPTSDLGKILACMHGLDVGGEINLTAAIQIAQLALKHRQNKNQRQRIIVFAGSPIKY 120

Query: 426 DEKELVKLAKRLKKEKVNCDVVSFGE-DSENNP-LLTTFVNTLNGKDTS 566
           ++K L  + KRLKK  V+ D+V+FGE D E  P  L   +  +N  D S
Sbjct: 121 EKKALEIVGKRLKKNSVSLDIVNFGEDDDEEKPQKLEALLTAVNNNDGS 169


>At1g05055.1 68414.m00506 basic transcription factor 2, 44kD
           subunit-related contains weak similarity to
           Swiss-Prot:Q13888 TFIIH basal transcription factor
           complex p44 subunit (Basic transcription factor 2 44 kDa
           subunit, BTF2-p44, General transcription factor IIH
           polypeptide 2) [Homo sapiens]
          Length = 421

 Score = 44.4 bits (100), Expect = 6e-05
 Identities = 33/151 (21%), Positives = 66/151 (43%), Gaps = 5/151 (3%)
 Frame = +3

Query: 69  KMVLESTMICVDNSDYMRNGDFLPTRLQAQQDAVNLVCHSKTRSNPENNVGLLTLANVEV 248
           K ++    I +D S      DF P+R+      V          NP + +GL+++ N  V
Sbjct: 78  KGLIRYLYIVIDFSRAAAEMDFRPSRMAIMAKHVEAFIREFFDQNPLSQIGLVSIKN-GV 136

Query: 249 LATLTSDVG-----RIMSKLHRVQPNGDINLLTGIRIAHLALKHRQGKNHKMRIVVFVGS 413
             TLT D+G      I + + +++  GD +L   + + H  L       H+  +++   +
Sbjct: 137 AHTLT-DLGGSPETHIKALMGKLEALGDSSLQNALELVHEHLNQVPSYGHR-EVLILYSA 194

Query: 414 PVNTDEKELVKLAKRLKKEKVNCDVVSFGED 506
               D  ++++  ++ KK K+ C V+    +
Sbjct: 195 LCTCDPGDIMETIQKCKKSKLRCSVIGLSAE 225


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,084,743
Number of Sequences: 28952
Number of extensions: 235522
Number of successful extensions: 576
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 570
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 575
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1121903184
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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