Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11f13f
(615 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putativ... 184 5e-47
At5g09300.2 68418.m01077 2-oxoisovalerate dehydrogenase, putativ... 179 2e-45
At5g09300.1 68418.m01078 2-oxoisovalerate dehydrogenase, putativ... 179 2e-45
At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alp... 47 1e-05
At1g01090.1 68414.m00011 pyruvate dehydrogenase E1 component alp... 41 6e-04
At1g24180.1 68414.m03050 pyruvate dehydrogenase E1 component alp... 36 0.021
At4g13890.1 68417.m02152 glycine hydroxymethyltransferase, putat... 33 0.15
At5g49660.1 68418.m06147 leucine-rich repeat transmembrane prote... 31 0.61
At5g58760.1 68418.m07360 transducin family protein / WD-40 repea... 25 1.0
At5g35200.1 68418.m04171 epsin N-terminal homology (ENTH) domain... 28 4.3
At3g49000.1 68416.m05352 RNA polymerase III subunit RPC82 family... 27 9.9
At1g04140.2 68414.m00404 transducin family protein / WD-40 repea... 27 9.9
At1g04140.1 68414.m00403 transducin family protein / WD-40 repea... 27 9.9
>At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putative /
3-methyl-2-oxobutanoate dehydrogenase, putative /
branched-chain alpha-keto acid dehydrogenase E1 alpha
subunit, putative similar to branched-chain alpha-keto
acid dehydrogenase E1-alpha subunit [Gallus gallus]
GI:12964598; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 472
Score = 184 bits (447), Expect = 5e-47
Identities = 86/157 (54%), Positives = 109/157 (69%)
Frame = +1
Query: 142 EFPGARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTMVQL 321
+FPG + Y SEMKF E+S IP YRV+D +G+II ++ + + + MY+ M L
Sbjct: 81 DFPGGKVGYTSEMKFIPESSSRRIPCYRVLDEDGRIIPDSDFIPVSEKLAVRMYEQMATL 140
Query: 322 SHMDKILYESQRQGRISFYMTNYGEEGIHIGSASALSPKDLVFSQYREVGVFLYRGMTVT 501
MD I YE+QRQGRISFY+T+ GEE I+I SA+ALSP D+V QYRE GV L+RG T+
Sbjct: 141 QVMDHIFYEAQRQGRISFYLTSVGEEAINIASAAALSPDDVVLPQYREPGVLLWRGFTLE 200
Query: 502 ELVNQCYGNCEDPGKGRQMPVHYGSKHHNMVTISSPL 612
E NQC+GN D GKGRQMP+HYGS N TISSP+
Sbjct: 201 EFANQCFGNKADYGKGRQMPIHYGSNRLNYFTISSPI 237
>At5g09300.2 68418.m01077 2-oxoisovalerate dehydrogenase, putative /
3-methyl-2-oxobutanoate dehydrogenase, putative /
branched-chain alpha-keto acid dehydrogenase E1 alpha
subunit, putative similar to branched-chain alpha-keto
acid dehydrogenase E1-alpha subunit [Gallus gallus]
GI:12964598; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 401
Score = 179 bits (435), Expect = 2e-45
Identities = 78/160 (48%), Positives = 111/160 (69%)
Frame = +1
Query: 133 KIAEFPGARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTM 312
++ +FPG + + E++F +E+ E +P YRV+D+NGQ+I ++ + + + +Y M
Sbjct: 7 QVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDM 66
Query: 313 VQLSHMDKILYESQRQGRISFYMTNYGEEGIHIGSASALSPKDLVFSQYREVGVFLYRGM 492
V L MD I YE+QRQGR+SFY T GEE I+I SA+AL+P+D++F QYRE GV L+RG
Sbjct: 67 VTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGF 126
Query: 493 TVTELVNQCYGNCEDPGKGRQMPVHYGSKHHNMVTISSPL 612
T+ E NQC+GN D GKGRQMPVHYGS N T+S+ +
Sbjct: 127 TLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATI 166
>At5g09300.1 68418.m01078 2-oxoisovalerate dehydrogenase, putative /
3-methyl-2-oxobutanoate dehydrogenase, putative /
branched-chain alpha-keto acid dehydrogenase E1 alpha
subunit, putative similar to branched-chain alpha-keto
acid dehydrogenase E1-alpha subunit [Gallus gallus]
GI:12964598; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 472
Score = 179 bits (435), Expect = 2e-45
Identities = 78/160 (48%), Positives = 111/160 (69%)
Frame = +1
Query: 133 KIAEFPGARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTM 312
++ +FPG + + E++F +E+ E +P YRV+D+NGQ+I ++ + + + +Y M
Sbjct: 78 QVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDM 137
Query: 313 VQLSHMDKILYESQRQGRISFYMTNYGEEGIHIGSASALSPKDLVFSQYREVGVFLYRGM 492
V L MD I YE+QRQGR+SFY T GEE I+I SA+AL+P+D++F QYRE GV L+RG
Sbjct: 138 VTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGF 197
Query: 493 TVTELVNQCYGNCEDPGKGRQMPVHYGSKHHNMVTISSPL 612
T+ E NQC+GN D GKGRQMPVHYGS N T+S+ +
Sbjct: 198 TLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATI 237
>At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alpha
subunit, mitochondrial (PDHE1-A) identical to SP|P52901
Pyruvate dehydrogenase E1 component alpha subunit,
mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
{Arabidopsis thaliana}
Length = 389
Score = 46.8 bits (106), Expect = 1e-05
Identities = 37/160 (23%), Positives = 70/160 (43%), Gaps = 9/160 (5%)
Frame = +1
Query: 163 PYVSEMKFFNETSYEPIPIYRVMDNNGQIIDK-NEEPNLDKATLINMYKTMVQLSHMDKI 339
P+ + T PI I + + D + L++ ++TM + M+ I
Sbjct: 16 PFSAAFSRLISTDTTPITIETSLPFTAHLCDPPSRSVESSSQELLDFFRTMALMRRME-I 74
Query: 340 LYESQRQGRI--SFYMTNYGEEGIHIGSASALSPKDLVFSQYREVGVFLYRGMTVTELVN 513
+S + ++ F G+E + IG +A++ KD + + YR+ +FL RG ++ E+ +
Sbjct: 75 AADSLYKAKLIRGFCHLYDGQEAVAIGMEAAITKKDAIITAYRDHCIFLGRGGSLHEVFS 134
Query: 514 QCYGNCEDPGKGRQMPVHYGSKH------HNMVTISSPLG 615
+ G KG+ +H+ K H +V PLG
Sbjct: 135 ELMGRQAGCSKGKGGSMHFYKKESSFYGGHGIVGAQVPLG 174
>At1g01090.1 68414.m00011 pyruvate dehydrogenase E1 component alpha
subunit, chloroplast identical to pyruvate dehydrogenase
E1 alpha subunit GB:AAB86803 GI:2454182 from
[Arabidopsis thaliana]; identical to cDNA pyruvate
dehydrogenase E1 alpha subunit mRNA, nuclear gene
encoding plastid protein GI:2454181
Length = 428
Score = 41.1 bits (92), Expect = 6e-04
Identities = 23/102 (22%), Positives = 51/102 (50%), Gaps = 1/102 (0%)
Frame = +1
Query: 292 INMYKTMVQLSHMDKILYESQRQGRISFYMTNY-GEEGIHIGSASALSPKDLVFSQYREV 468
+ +Y+ M+ + + + +G++ ++ Y G+E + G L+ D V S YR+
Sbjct: 85 LELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKSDSVVSTYRDH 144
Query: 469 GVFLYRGMTVTELVNQCYGNCEDPGKGRQMPVHYGSKHHNMV 594
L +G++ ++++ +G +G+ +H SK HNM+
Sbjct: 145 VHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEHNML 186
>At1g24180.1 68414.m03050 pyruvate dehydrogenase E1 component alpha
subunit, mitochondrial, putative similar to SP|P52901
Pyruvate dehydrogenase E1 component alpha subunit,
mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
{Arabidopsis thaliana}; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 393
Score = 35.9 bits (79), Expect = 0.021
Identities = 20/81 (24%), Positives = 38/81 (46%), Gaps = 6/81 (7%)
Frame = +1
Query: 391 GEEGIHIGSASALSPKDLVFSQYREVGVFLYRGMTVTELVNQCYGNCEDPGKGRQMPVHY 570
G+E + +G +A++ KD + + YR+ F+ RG + + ++ G G+ +H+
Sbjct: 98 GQEALAVGMEAAITKKDAIITSYRDHCTFIGRGGKLVDAFSELMGRKTGCSHGKGGSMHF 157
Query: 571 GSKH------HNMVTISSPLG 615
K H +V PLG
Sbjct: 158 YKKDASFYGGHGIVGAQIPLG 178
>At4g13890.1 68417.m02152 glycine hydroxymethyltransferase, putative
/ serine hydroxymethyltransferase, putative /
serine/threonine aldolase, putative similar to serine
hydroxymethyltransferase [Chlamydomonas reinhardtii]
GI:17066746; contains Pfam profile PF00464: serine
hydroxymethyltransferase
Length = 470
Score = 33.1 bits (72), Expect = 0.15
Identities = 17/51 (33%), Positives = 31/51 (60%), Gaps = 1/51 (1%)
Frame = +1
Query: 400 GIHIGSASALSPKDLVFSQYREVGVFLYRGMTVTELVNQCYGN-CEDPGKG 549
G+ IG+ A++ + LV + ++G FL+R +T+T + + YG +D KG
Sbjct: 387 GVRIGTP-AMTSRGLVEKDFEKIGEFLHRAVTITLDIQEQYGKVMKDFNKG 436
>At5g49660.1 68418.m06147 leucine-rich repeat transmembrane protein
kinase, putative contains leucine rich repeat (LRR)
domains, Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 966
Score = 31.1 bits (67), Expect = 0.61
Identities = 18/67 (26%), Positives = 35/67 (52%)
Frame = +1
Query: 130 GKIAEFPGARAPYVSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKT 309
G + +F ++ V +M + + T P+ I+ + D + ++ NE P LD TL +
Sbjct: 136 GTLPDFSQMKSLRVIDMSWNHFTGSFPLSIFNLTDL--EYLNFNENPELDLWTLPDSVSK 193
Query: 310 MVQLSHM 330
+ +L+HM
Sbjct: 194 LTKLTHM 200
>At5g58760.1 68418.m07360 transducin family protein / WD-40 repeat
family protein contains 4 WD-40 repeats (PF00400);
damage-specific DNA binding protein 2 (GI:10798819)
[Homo sapiens]
Length = 557
Score = 25.4 bits (53), Expect(2) = 1.0
Identities = 14/44 (31%), Positives = 25/44 (56%)
Frame = +1
Query: 166 YVSEMKFFNETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLIN 297
Y+SE +N T+ PI + +NGQ++ + +PN+ T +N
Sbjct: 431 YISEN--YNGTALHPIDF--IDASNGQLVAEVMDPNITTITPVN 470
Score = 23.4 bits (48), Expect(2) = 1.0
Identities = 15/54 (27%), Positives = 25/54 (46%), Gaps = 8/54 (14%)
Frame = +1
Query: 409 IGSASALSPKDLVFSQYREVGVFLYRGMTVTELVNQ--------CYGNCEDPGK 546
I + L P+D V + +F++R TE+V + CYG+ + GK
Sbjct: 466 ITPVNKLHPRDDVLASGSSRSLFIWRPQDNTEMVEEKKDKKIIICYGDSKKKGK 519
>At5g35200.1 68418.m04171 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain; similar
to clathrin assembly protein short form, Rattus
norvegicus, EMBL:AF041373;similar to Chain A, Calm-N
N-Terminal Domain Of Clathrin Assembly Lymphoid Myeloid
Leukaemia Protein, Pi(4,5)p2 Complex (GP:13399999) {Homo
sapiens}
Length = 544
Score = 28.3 bits (60), Expect = 4.3
Identities = 15/60 (25%), Positives = 24/60 (40%)
Frame = +1
Query: 190 NETSYEPIPIYRVMDNNGQIIDKNEEPNLDKATLINMYKTMVQLSHMDKILYESQRQGRI 369
N T +E + N G D LDK TL ++Y+ +++S Y Q +
Sbjct: 401 NSTGWELALVTAPSSNEGAAADSKLAGGLDKLTLDSLYEDAIRVSQQQNRSYNPWEQNPV 460
>At3g49000.1 68416.m05352 RNA polymerase III subunit RPC82 family
protein contains Pfam profile: PF05645 RNA polymerase
III subunit RPC82
Length = 523
Score = 27.1 bits (57), Expect = 9.9
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -3
Query: 364 GLVFVIHIEFYPYGSVVPWFCTC 296
G+V+ IHI +GSVV C C
Sbjct: 7 GIVYAIHIITVQFGSVVSKVCEC 29
>At1g04140.2 68414.m00404 transducin family protein / WD-40 repeat
family protein contains 4 WD-40 repeats (PF00400);
similar to neural cell adhesion molecule 2, large
isoform precursor gb|M76710 from Xenopus laevis, and
beta transducin from S. cerevisiae gb|Q05946. ESTs
gb|N65081 gb|Z30910, gb|Z34190, gb|Z34611, gb|R30101,
gb|H36304, and gb|N65606 come from
Length = 793
Score = 27.1 bits (57), Expect = 9.9
Identities = 15/37 (40%), Positives = 17/37 (45%)
Frame = -1
Query: 591 HVVVLASVMDRHLSPFPRILAVSVTLVHKFRHSHPSV 481
H VL S M H SP R LA V V + PS+
Sbjct: 583 HHAVLCSEMGAHFSPCGRYLAACVACVIPHAETDPSL 619
>At1g04140.1 68414.m00403 transducin family protein / WD-40 repeat
family protein contains 4 WD-40 repeats (PF00400);
similar to neural cell adhesion molecule 2, large
isoform precursor gb|M76710 from Xenopus laevis, and
beta transducin from S. cerevisiae gb|Q05946. ESTs
gb|N65081 gb|Z30910, gb|Z34190, gb|Z34611, gb|R30101,
gb|H36304, and gb|N65606 come from
Length = 790
Score = 27.1 bits (57), Expect = 9.9
Identities = 15/37 (40%), Positives = 17/37 (45%)
Frame = -1
Query: 591 HVVVLASVMDRHLSPFPRILAVSVTLVHKFRHSHPSV 481
H VL S M H SP R LA V V + PS+
Sbjct: 583 HHAVLCSEMGAHFSPCGRYLAACVACVIPHAETDPSL 619
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,009,391
Number of Sequences: 28952
Number of extensions: 296210
Number of successful extensions: 737
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 722
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 737
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1236350304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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