BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11e22f (594 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccha... 232 2e-62 SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosacchar... 231 4e-62 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 28 0.89 SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16 |S... 28 1.2 SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 28 1.2 SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharom... 27 2.1 SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosac... 27 2.1 SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 26 3.6 SPAC139.03 |||transcription factor, zf-fungal binuclear cluster ... 26 4.8 SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 26 4.8 SPAC30D11.08c |phf2|swp2, saf60|PHD finger containing protein Ph... 25 6.3 SPAC25H1.06 |||histone acetyltransferase complex subunit |Schizo... 25 8.3 >SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 232 bits (568), Expect = 2e-62 Identities = 117/190 (61%), Positives = 138/190 (72%) Frame = +1 Query: 25 MGISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRL 204 MGI+RD HKR ATG KRA RKKRK+ELGR +NTR+GP+RIH VR RGGN K+RALRL Sbjct: 1 MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60 Query: 205 DTGNFSWGSECSTRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLP 384 D+GNFSWGSE ++KTRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY + Sbjct: 61 DSGNFSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGIL 120 Query: 385 LGRKKGAKLTEAEEAIINKKRSQKTARKYLARQRLAKVEGALEEQFHTGRLLACVASRPG 564 +G KG K T +S+ RK+ AR +KV+ ALE QF GRL A V+SRPG Sbjct: 121 MG-SKGKKATSTP-----NPKSKHVQRKHSARLGDSKVDSALETQFAAGRLYAVVSSRPG 174 Query: 565 QCGRADGYIL 594 Q GR DGYIL Sbjct: 175 QSGRCDGYIL 184 >SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 231 bits (566), Expect = 4e-62 Identities = 117/190 (61%), Positives = 138/190 (72%) Frame = +1 Query: 25 MGISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRL 204 MGI+RD HKR ATG KRA RKKRK+ELGR +NTR+GP+RIH VR RGGN K+RALRL Sbjct: 1 MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60 Query: 205 DTGNFSWGSECSTRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLP 384 D+GNFSWGSE ++KTRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY + Sbjct: 61 DSGNFSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGIL 120 Query: 385 LGRKKGAKLTEAEEAIINKKRSQKTARKYLARQRLAKVEGALEEQFHTGRLLACVASRPG 564 +G KG K T +S+ RK+ AR +KV+ ALE QF GRL A V+SRPG Sbjct: 121 MG-SKGKKATATP-----TPKSKHVQRKHSARLGDSKVDSALETQFAAGRLYAVVSSRPG 174 Query: 565 QCGRADGYIL 594 Q GR DGYIL Sbjct: 175 QSGRCDGYIL 184 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 28.3 bits (60), Expect = 0.89 Identities = 22/76 (28%), Positives = 33/76 (43%), Gaps = 2/76 (2%) Frame = +1 Query: 37 RDHWHKRRATGGKRAPIRKKRKY--ELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDT 210 RD +K + G K+ + EL +N + Q+ + S T YRA+ LDT Sbjct: 1083 RDRLYKSQLIGRVLDDTTKENRLLKELASSKSNITIRWQQGGLIGSGSFGTVYRAVNLDT 1142 Query: 211 GNFSWGSECSTRKTRI 258 G+ E + K RI Sbjct: 1143 GDLMAVKEVALHKPRI 1158 >SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16 |Schizosaccharomyces pombe|chr 1|||Manual Length = 672 Score = 27.9 bits (59), Expect = 1.2 Identities = 26/83 (31%), Positives = 39/83 (46%) Frame = +1 Query: 118 PAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNFSWGSECSTRKTRIIDVVYNASNNELV 297 P NT + PQ RSR T R+L +G S S ++ +TR V +AS+N L+ Sbjct: 530 PLQNTVISPQPTR--RSR---TPIRSL---SGRSSVASSRNSSRTRSYSNVSSASSNSLI 581 Query: 298 RTKTLVKNAIVVVDATPFRQWYE 366 + ++ V TPF +E Sbjct: 582 SIISSKPSSSTVQRQTPFHSPFE 604 >SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 207 Score = 27.9 bits (59), Expect = 1.2 Identities = 17/54 (31%), Positives = 26/54 (48%) Frame = +1 Query: 40 DHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALR 201 + W K T K+ +KRK L R A RL + + S+ S+GG +A + Sbjct: 32 EKWSKGVKTN-KKEQEAEKRKAALERKAERERLEKEEMESLPSKGGKGSKKAAK 84 >SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1021 Score = 27.1 bits (57), Expect = 2.1 Identities = 16/73 (21%), Positives = 33/73 (45%) Frame = +1 Query: 340 ATPFRQWYESHYTLPLGRKKGAKLTEAEEAIINKKRSQKTARKYLARQRLAKVEGALEEQ 519 +TP + H KKG+K+ A++ ++R + AR+ A + K ++E+ Sbjct: 754 STPDTTGSDIHIVSEKRGKKGSKVITAKKVSAKERREARRARRQTALEESLKAPISIEDA 813 Query: 520 FHTGRLLACVASR 558 +LA + + Sbjct: 814 TDPQTILAILKQK 826 >SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 27.1 bits (57), Expect = 2.1 Identities = 23/88 (26%), Positives = 33/88 (37%), Gaps = 5/88 (5%) Frame = +1 Query: 40 DHWHKRRATGGKRAPIRKKRKY-----ELGRPAANTRLGPQRIHSVRSRGGNTKYRALRL 204 D W +A G +PIR+ ++Y + +PA R H R K R + + Sbjct: 758 DEWKASKAFGANDSPIRENKRYKHNKLQTPKPADKFR---DNYHKQNKRNREAKERGIGI 814 Query: 205 DTGNFSWGSECSTRKTRIIDVVYNASNN 288 N S RK R + A NN Sbjct: 815 KV-NSELKSAVEIRKARELKEKRLAKNN 841 >SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 817 Score = 26.2 bits (55), Expect = 3.6 Identities = 15/61 (24%), Positives = 31/61 (50%), Gaps = 2/61 (3%) Frame = +1 Query: 223 WGSEC--STRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRK 396 WGS+ +K + +V+ +++ + +T L AT F + +S+Y +PL R+ Sbjct: 250 WGSQSYLQEKKAGLAEVLNISTSQQKPKTTALDYEVFRHEMATKFSSFADSYYPVPLDRE 309 Query: 397 K 399 + Sbjct: 310 Q 310 >SPAC139.03 |||transcription factor, zf-fungal binuclear cluster type |Schizosaccharomyces pombe|chr 1|||Manual Length = 625 Score = 25.8 bits (54), Expect = 4.8 Identities = 15/39 (38%), Positives = 19/39 (48%) Frame = +1 Query: 13 DPTKMGISRDHWHKRRATGGKRAPIRKKRKYELGRPAAN 129 + TK G + RRA A RKK K + GRP +N Sbjct: 3 ETTKSGSKKSGQTSRRAIHSCLACRRKKLKCDHGRPCSN 41 >SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosaccharomyces pombe|chr 2|||Manual Length = 735 Score = 25.8 bits (54), Expect = 4.8 Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = -2 Query: 362 YHCLNGVASTTTIAFLTRVFVRTNS 288 ++C N V S T + + VFVR NS Sbjct: 518 FYCSNEVLSLTALLSVPNVFVRPNS 542 >SPAC30D11.08c |phf2|swp2, saf60|PHD finger containing protein Phf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 538 Score = 25.4 bits (53), Expect = 6.3 Identities = 13/29 (44%), Positives = 16/29 (55%) Frame = -2 Query: 284 LDALYTTSMIRVLRVEHSDPQEKLPVSRR 198 L+ + T S +V R H DP KLP RR Sbjct: 193 LEGVKTKSGRKVHRPNHFDPLVKLPTRRR 221 >SPAC25H1.06 |||histone acetyltransferase complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 408 Score = 25.0 bits (52), Expect = 8.3 Identities = 12/40 (30%), Positives = 23/40 (57%), Gaps = 1/40 (2%) Frame = -1 Query: 588 VTIGATTLTWATRHASQ-QTPCVELLF*STLNLSKTLPCQ 472 +T+G T+L+W+ RH+ + + C E + N +K P + Sbjct: 329 LTLGGTSLSWSWRHSGRIVSACQEYCY--VWNFNKANPLE 366 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,460,947 Number of Sequences: 5004 Number of extensions: 48232 Number of successful extensions: 151 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 144 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 149 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 258201856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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