BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11e10f (543 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 38 0.001 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 33 0.027 SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 27 2.4 SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 26 3.1 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 26 3.1 SPBC577.13 |syj2||inositol-polyphosphate 5-phosphatase |Schizosa... 26 4.1 SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizo... 25 5.5 SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ... 25 5.5 SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein Sap155|Sc... 25 7.2 SPAC688.14 |||lysine methyltransferase |Schizosaccharomyces pomb... 25 7.2 SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc... 25 9.5 SPCC962.01 ||SPCP31B10.09|C2 domain protein|Schizosaccharomyces ... 25 9.5 SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 25 9.5 SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 25 9.5 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 37.9 bits (84), Expect = 0.001 Identities = 19/78 (24%), Positives = 40/78 (51%) Frame = +3 Query: 294 NVNQALTPSPVEINPEPVIVDEESEIKPDPVIVVDGPEVTPVIIADPAIVLPEPVIVAPI 473 +++++ + S + +P P++ D ++ +++ P P PA+++P P API Sbjct: 696 DLDESFSGSEISSSPSPLLPDVSDTVEEQQKLLLKSPPPPP-----PAVIVPTPA-PAPI 749 Query: 474 EPTPVSPVIIGAPDLPNP 527 P +P++ G P P P Sbjct: 750 PVPPPAPIMGGPPPPPPP 767 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 33.1 bits (72), Expect = 0.027 Identities = 29/92 (31%), Positives = 43/92 (46%), Gaps = 5/92 (5%) Frame = +3 Query: 267 ASPLVQIIVNVNQALTPSPVEINPEPVIVDEESEIKPDPVIV-VDGPEVTPVIIADPAIV 443 A+P+V +V Q P+ V + PE + V + P + V P V PV+ P++ Sbjct: 549 AAPVVPEAPSVPQR--PA-VPVVPEALSVPQPPVAPVAPEVPSVPQPPVAPVVPEAPSVP 605 Query: 444 LPEPVIVAPIEPT----PVSPVIIGAPDLPNP 527 P VAP P+ P PV+ AP +P P Sbjct: 606 QPPVAPVAPEVPSVPQRPAVPVVPEAPSVPQP 637 Score = 30.7 bits (66), Expect = 0.15 Identities = 29/96 (30%), Positives = 47/96 (48%), Gaps = 9/96 (9%) Frame = +3 Query: 267 ASPLVQIIVNVNQALTPSPVEINPEPVIVDEESEIKPDPVIVVDGPEVT--PVIIADP-A 437 A+P+V +V+Q P + PE + +P +V + P V P + P A Sbjct: 519 AAPVVPEAPSVHQ---PPAAPVAPEVPSAPQ----RPAAPVVPEAPSVPQRPAVPVVPEA 571 Query: 438 IVLPEPVI--VAPIEPT----PVSPVIIGAPDLPNP 527 + +P+P + VAP P+ PV+PV+ AP +P P Sbjct: 572 LSVPQPPVAPVAPEVPSVPQPPVAPVVPEAPSVPQP 607 Score = 29.9 bits (64), Expect = 0.25 Identities = 24/91 (26%), Positives = 37/91 (40%), Gaps = 6/91 (6%) Frame = +3 Query: 273 PLVQIIVNVNQALTPSPVEINPEPVIVDEE--SEIKPDPVIVVDGPEVTPVIIADPAIVL 446 P+ + V P + PE V + + + P+ V P V PV+ P++ Sbjct: 578 PVAPVAPEVPSVPQPPVAPVVPEAPSVPQPPVAPVAPEVPSVPQRPAV-PVVPEAPSVPQ 636 Query: 447 PEPVIVAPIEPT----PVSPVIIGAPDLPNP 527 P V P P+ P PV+ AP +P P Sbjct: 637 PPAAPVVPEVPSVPQRPAVPVVPEAPSVPQP 667 Score = 29.1 bits (62), Expect = 0.44 Identities = 28/91 (30%), Positives = 41/91 (45%), Gaps = 6/91 (6%) Frame = +3 Query: 267 ASPLVQIIVNVNQA-LTPSPVEIN--PEPVI--VDEESEIKPDPVIVVDGPEVTPVIIAD 431 A P+V ++V Q + P E+ P+P + V E+ P P + PEV P + Sbjct: 564 AVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAPVVPEAPSVPQPPVAPVAPEV-PSVPQR 622 Query: 432 PAI-VLPEPVIVAPIEPTPVSPVIIGAPDLP 521 PA+ V+PE V PV P + P P Sbjct: 623 PAVPVVPEAPSVPQPPAAPVVPEVPSVPQRP 653 Score = 26.2 bits (55), Expect = 3.1 Identities = 21/71 (29%), Positives = 28/71 (39%) Frame = +3 Query: 309 LTPSPVEINPEPVIVDEESEIKPDPVIVVDGPEVTPVIIADPAIVLPEPVIVAPIEPTPV 488 +TPS + PV+ + S +P V PEV A V+PE V PV Sbjct: 510 ITPSVPQPPAAPVVPEAPSVHQPPAAPVA--PEVPSAPQRPAAPVVPEAPSVPQRPAVPV 567 Query: 489 SPVIIGAPDLP 521 P + P P Sbjct: 568 VPEALSVPQPP 578 Score = 25.8 bits (54), Expect = 4.1 Identities = 23/70 (32%), Positives = 31/70 (44%), Gaps = 1/70 (1%) Frame = +3 Query: 315 PSPVEINPEPVIVDEESEIKPDPVIVVDGPEVTPVIIADPAI-VLPEPVIVAPIEPTPVS 491 PS + PV+ + S +P VV PEV P + PA+ V+PE V PV Sbjct: 617 PSVPQRPAVPVVPEAPSVPQPPAAPVV--PEV-PSVPQRPAVPVVPEAPSVPQPPAAPVV 673 Query: 492 PVIIGAPDLP 521 P + P P Sbjct: 674 PEVPSVPQPP 683 >SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1029 Score = 26.6 bits (56), Expect = 2.4 Identities = 8/26 (30%), Positives = 16/26 (61%) Frame = -2 Query: 452 LRKNNGGISNDYGRNFRTINNDYGIR 375 L+ +G + ND NF ++ ++G+R Sbjct: 818 LKNYDGDVENDMALNFTVVHEEFGVR 843 >SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharomyces pombe|chr 2|||Manual Length = 1016 Score = 26.2 bits (55), Expect = 3.1 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +3 Query: 402 PEVTPVIIADPAIVLPEPVIVAPIEPTPV 488 P +TP ADP P+P+ + P++ PV Sbjct: 586 PSITP---ADPVPTSPKPLAIEPVKRKPV 611 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 26.2 bits (55), Expect = 3.1 Identities = 24/74 (32%), Positives = 30/74 (40%), Gaps = 3/74 (4%) Frame = +3 Query: 315 PSPVEINPEPVIVDEESEIKPD---PVIVVDGPEVTPVIIADPAIVLPEPVIVAPIEPTP 485 P+P P P KP P + V PV A +P+P + AP P Sbjct: 1077 PAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAP----P 1132 Query: 486 VSPVIIGAPDLPNP 527 V PV GAP +P P Sbjct: 1133 V-PVPSGAPPVPKP 1145 Score = 25.8 bits (54), Expect = 4.1 Identities = 14/38 (36%), Positives = 19/38 (50%) Frame = +3 Query: 414 PVIIADPAIVLPEPVIVAPIEPTPVSPVIIGAPDLPNP 527 PV + A +P+P + AP P P GAP +P P Sbjct: 1132 PVPVPSGAPPVPKPSVAAPPVPAP-----SGAPPVPKP 1164 Score = 25.8 bits (54), Expect = 4.1 Identities = 18/42 (42%), Positives = 23/42 (54%) Frame = +2 Query: 218 PRSSFPSRHWSHRPRPGFSPSSDYC*CKPSINSLSRRNKSRA 343 PRSS PS H + P P SS P+ S+S R+KS+A Sbjct: 1252 PRSSVPSPHSNASPSP---TSSSMASAAPARTSVS-RSKSKA 1289 >SPBC577.13 |syj2||inositol-polyphosphate 5-phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 889 Score = 25.8 bits (54), Expect = 4.1 Identities = 13/21 (61%), Positives = 17/21 (80%), Gaps = 1/21 (4%) Frame = -3 Query: 487 TGVGSIGATITGSGR-TMAGS 428 TGVGSIG++ T GR ++AGS Sbjct: 483 TGVGSIGSSATRRGRKSIAGS 503 >SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizosaccharomyces pombe|chr 1|||Manual Length = 335 Score = 25.4 bits (53), Expect = 5.5 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = +3 Query: 438 IVLPEPVIVAPIEPTPVSPVIIGAPDLPN 524 +V P P + P+P + I GAP +PN Sbjct: 288 LVPPPPPGMVMASPSPAAATIPGAPVMPN 316 >SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces pombe|chr 3|||Manual Length = 1364 Score = 25.4 bits (53), Expect = 5.5 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = +2 Query: 167 NHQRIRANRQRTRYRRLPRSSFPSRH 244 NH+R ++ + P SS PSRH Sbjct: 594 NHERSPNGNNNLKFSKDPNSSSPSRH 619 >SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein Sap155|Schizosaccharomyces pombe|chr 1|||Manual Length = 1188 Score = 25.0 bits (52), Expect = 7.2 Identities = 8/14 (57%), Positives = 11/14 (78%) Frame = -1 Query: 285 SELGEKPGRGRWDQ 244 +E+ +KP R RWDQ Sbjct: 208 TEISQKPKRSRWDQ 221 >SPAC688.14 |||lysine methyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 461 Score = 25.0 bits (52), Expect = 7.2 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = +3 Query: 300 NQALTPSPVEINPEPVIVDEESEIKPD 380 +Q+L + E+N E D ESE+ PD Sbjct: 219 SQSLVGNNCEVNSEDEFSDLESEVDPD 245 >SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosaccharomyces pombe|chr 2|||Manual Length = 658 Score = 24.6 bits (51), Expect = 9.5 Identities = 13/39 (33%), Positives = 19/39 (48%) Frame = +3 Query: 264 QASPLVQIIVNVNQALTPSPVEINPEPVIVDEESEIKPD 380 Q SPLV N + SPV + P+ + E I+P+ Sbjct: 285 QPSPLVSSKSTDNIIRSHSPVLLTPQTLSTSETKHIRPN 323 >SPCC962.01 ||SPCP31B10.09|C2 domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1429 Score = 24.6 bits (51), Expect = 9.5 Identities = 11/34 (32%), Positives = 20/34 (58%), Gaps = 1/34 (2%) Frame = -3 Query: 526 GLGRSGAPMMTGDTGVGSIGA-TITGSGRTMAGS 428 G+G +G + G T +GS+G+ +TG + G+ Sbjct: 1225 GVGAAGGLALAGVTTIGSVGSKAVTGVADGVTGT 1258 >SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe|chr 1|||Manual Length = 560 Score = 24.6 bits (51), Expect = 9.5 Identities = 20/49 (40%), Positives = 24/49 (48%), Gaps = 5/49 (10%) Frame = +3 Query: 321 PVEI-NPEPVI----VDEESEIKPDPVIVVDGPEVTPVIIADPAIVLPE 452 PVEI +PE + VDE P+ V D E P +I DP V PE Sbjct: 239 PVEIYDPEDIKPADWVDEPEIPDPNAVKPDDWDEDAPRMIPDPDAVKPE 287 >SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 24.6 bits (51), Expect = 9.5 Identities = 10/19 (52%), Positives = 12/19 (63%) Frame = +3 Query: 399 GPEVTPVIIADPAIVLPEP 455 GP+VT V + DP I L P Sbjct: 82 GPQVTTVQVGDPVIALYTP 100 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,765,023 Number of Sequences: 5004 Number of extensions: 35316 Number of successful extensions: 152 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 127 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 143 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 223909422 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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