BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11d24r (780 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g59370.1 68416.m06621 expressed protein hypothetical protein ... 33 0.16 At5g14280.1 68418.m01670 DNA-binding storekeeper protein-related... 33 0.28 At4g02510.1 68417.m00343 chloroplast outer membrane protein, put... 32 0.49 At3g55700.1 68416.m06188 UDP-glucoronosyl/UDP-glucosyl transfera... 31 0.86 At5g31412.1 68418.m03722 hypothetical protein contains Pfam doma... 31 1.1 At4g25940.1 68417.m03731 epsin N-terminal homology (ENTH) domain... 30 2.0 At4g21450.2 68417.m03102 vesicle-associated membrane family prot... 30 2.0 At4g21450.1 68417.m03103 vesicle-associated membrane family prot... 30 2.0 At1g44090.1 68414.m05093 gibberellin 20-oxidase family protein s... 30 2.0 At2g45000.1 68415.m05603 expressed protein contains Pfam profile... 29 2.6 At1g64790.1 68414.m07346 translational activator family protein ... 29 3.5 At1g53720.1 68414.m06113 cyclophilin-RNA interacting protein, pu... 29 3.5 At5g45830.1 68418.m05637 expressed protein low similarity to tum... 29 4.6 At3g57660.1 68416.m06424 DNA-directed RNA polymerase family prot... 29 4.6 At1g56520.1 68414.m06499 disease resistance protein (TIR-NBS-LRR... 28 6.0 At1g55820.1 68414.m06400 hydroxyproline-rich glycoprotein family... 28 8.0 >At3g59370.1 68416.m06621 expressed protein hypothetical protein F12F1.4 - Arabidopsis thaliana, EMBL:AC002131 Length = 95 Score = 33.5 bits (73), Expect = 0.16 Identities = 23/85 (27%), Positives = 38/85 (44%) Frame = -2 Query: 587 VDNVDAPVEDADLQVAPVNIVDEPVEKTNEEIENDSEVGSISWVEYPAELDEDGNGLVIV 408 V +V + + ++ P + E EKTNE+ E + V E P ++E+ + Sbjct: 9 VTSVAVEIVETEVNQHPEEVFVEDAEKTNEDEEEKAAV----ITETPTVVEEEKKAEEVT 64 Query: 407 DLPIEAQPEDLEKAQLVDLPVENVA 333 + P E + E LE+ Q E VA Sbjct: 65 ETPEEKKTEALEEKQTEVAAAEEVA 89 >At5g14280.1 68418.m01670 DNA-binding storekeeper protein-related contains similarity to storekeeper protein [Solanum tuberosum] gi|14268476|emb|CAC39398; contains PF04504: Protein of unknown function, DUF573 Length = 572 Score = 32.7 bits (71), Expect = 0.28 Identities = 16/57 (28%), Positives = 27/57 (47%) Frame = -2 Query: 566 VEDADLQVAPVNIVDEPVEKTNEEIENDSEVGSISWVEYPAELDEDGNGLVIVDLPI 396 VE+ L + + + E N++ + S +SWVE P + + + I DLPI Sbjct: 277 VEELKLNINKLRFSAKLAEAANDDYSSSSSSSIVSWVEIPISMSINIGEISIPDLPI 333 >At4g02510.1 68417.m00343 chloroplast outer membrane protein, putative similar to chloroplast protein import component Toc159 [Pisum sativum] GI:8489806, chloroplast outer envelope protein 86 [Pisum sativum] GI:599958, GTP-binding protein [Pisum sativum] GI:576509 Length = 1503 Score = 31.9 bits (69), Expect = 0.49 Identities = 19/59 (32%), Positives = 31/59 (52%) Frame = -2 Query: 491 ENDSEVGSISWVEYPAELDEDGNGLVIVDLPIEAQPEDLEKAQLVDLPVENVAEPEDLS 315 E DS V S A+++ G+V+V EA ++ +K VD + N+ EP+DL+ Sbjct: 462 EGDSMVDSSVVDSVDADINVAEPGVVVVGAAKEAVIKEDDKDDEVDKTISNIEEPDDLT 520 >At3g55700.1 68416.m06188 UDP-glucoronosyl/UDP-glucosyl transferase family protein glucuronosyl transferase homolog, Lycopersicon esculentum, PIR:S39507 ;contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl transferase Length = 460 Score = 31.1 bits (67), Expect = 0.86 Identities = 23/68 (33%), Positives = 36/68 (52%), Gaps = 1/68 (1%) Frame = -2 Query: 473 GSISWVEYPA-ELDEDGNGLVIVDLPIEAQPEDLEKAQLVDLPVENVAEPEDLSPVQVVN 297 G+ S+ + A L D L I D ++ +L ++ DLPV EPE+L +VVN Sbjct: 141 GASSFCAFAAFPLLRDKGYLPIQDSRLDEPVTELPPLKVKDLPVMETNEPEEL--YRVVN 198 Query: 296 PIVENSQS 273 +VE ++S Sbjct: 199 DMVEGAKS 206 >At5g31412.1 68418.m03722 hypothetical protein contains Pfam domain, PF04937: Protein of unknown function (DUF 659) Length = 433 Score = 30.7 bits (66), Expect = 1.1 Identities = 17/51 (33%), Positives = 26/51 (50%) Frame = -2 Query: 455 EYPAELDEDGNGLVIVDLPIEAQPEDLEKAQLVDLPVENVAEPEDLSPVQV 303 E P+E DE+ NG + P E EDLE + D+ ++ E ++ V V Sbjct: 375 EGPSERDEEPNGEPVYQTPNEKPREDLESHEEHDITIDIEFESDNEQVVNV 425 >At4g25940.1 68417.m03731 epsin N-terminal homology (ENTH) domain-containing protein contains Pfam PF01417: ENTH domain. ENTH (Epsin N-terminal homology) domain; similar to Chain B, Crystal Structure Of N-Terminal Domain Of Drosophila Ap180 (GP:13399617) [Drosophila melanogaster]; supporting cDNA gi|20465326|gb|AY096427.1| Length = 601 Score = 29.9 bits (64), Expect = 2.0 Identities = 22/73 (30%), Positives = 31/73 (42%) Frame = -2 Query: 512 EKTNEEIENDSEVGSISWVEYPAELDEDGNGLVIVDLPIEAQPEDLEKAQLVDLPVENVA 333 EK EE E + S+ E PAE D IE + ED EK + D + Sbjct: 331 EKEEEEQEEEEAEHSVQ-PEEPAEADNQKENSEGDQPLIEEEEEDQEKIEEEDAKPSFLI 389 Query: 332 EPEDLSPVQVVNP 294 + +DL + +NP Sbjct: 390 DTDDLLGLNEINP 402 >At4g21450.2 68417.m03102 vesicle-associated membrane family protein / VAMP family protein similar to VAP27 GI:6688926 [Nicotiana plumbaginifolia] Length = 212 Score = 29.9 bits (64), Expect = 2.0 Identities = 14/32 (43%), Positives = 19/32 (59%) Frame = +1 Query: 370 FSKSSGCASIGRSTITSPFPSSSNSAGYSTQL 465 F SSG + S TSPFPS ++S+ S+ L Sbjct: 23 FRNSSGHRNAASSAATSPFPSGASSSSTSSHL 54 >At4g21450.1 68417.m03103 vesicle-associated membrane family protein / VAMP family protein similar to VAP27 GI:6688926 [Nicotiana plumbaginifolia] Length = 295 Score = 29.9 bits (64), Expect = 2.0 Identities = 14/32 (43%), Positives = 19/32 (59%) Frame = +1 Query: 370 FSKSSGCASIGRSTITSPFPSSSNSAGYSTQL 465 F SSG + S TSPFPS ++S+ S+ L Sbjct: 23 FRNSSGHRNAASSAATSPFPSGASSSSTSSHL 54 >At1g44090.1 68414.m05093 gibberellin 20-oxidase family protein similar to gibberellin 20-oxidase GI:4164141 from [Lactuca sativa]; contains Pfam domain PF03171 2OG-Fe(II) oxygenase superfamily Length = 385 Score = 29.9 bits (64), Expect = 2.0 Identities = 16/46 (34%), Positives = 25/46 (54%), Gaps = 1/46 (2%) Frame = -2 Query: 308 QVVNPIVENSQSEYPGKRYPDATWR*IIYFS-SFYLN*NIYIHNFS 174 +++ P E + E ++YPD TW + F+ S Y N +HNFS Sbjct: 328 KIIVPPPELVEGEEASRKYPDFTWAQLQKFTQSGYRVDNTTLHNFS 373 >At2g45000.1 68415.m05603 expressed protein contains Pfam profile: PF05064 Nsp1-like C-terminal region Length = 739 Score = 29.5 bits (63), Expect = 2.6 Identities = 22/50 (44%), Positives = 28/50 (56%), Gaps = 2/50 (4%) Frame = +1 Query: 322 SSGSATFSTGRSTSW--AFSKSSGCASIGRSTITSPFPSSSNSAGYSTQL 465 SS S +TG S S+ A S S SI +T +SPF SS+SAG + L Sbjct: 243 SSSSIFGATGSSPSFSVASSASGSSPSIFGATGSSPFFGSSSSAGSTPSL 292 >At1g64790.1 68414.m07346 translational activator family protein similar to HsGCN1 [Homo sapiens] GI:2282576 Length = 2440 Score = 29.1 bits (62), Expect = 3.5 Identities = 16/60 (26%), Positives = 28/60 (46%) Frame = -3 Query: 532 TSWMNQLKRRMRKSRMTVKLARSAGLNILLNWTKMETDLLLLIYLLRHNPKI*RKPSLLT 353 TS + L + + ++++V L L+ NW +L + LL+HNP SL + Sbjct: 2224 TSMLGVLSQYLEAAQLSVLLQEVNDLSASQNWGARHGSVLCISSLLKHNPSTIMTSSLFS 2283 >At1g53720.1 68414.m06113 cyclophilin-RNA interacting protein, putative Length = 506 Score = 29.1 bits (62), Expect = 3.5 Identities = 26/99 (26%), Positives = 44/99 (44%) Frame = -2 Query: 578 VDAPVEDADLQVAPVNIVDEPVEKTNEEIENDSEVGSISWVEYPAELDEDGNGLVIVDLP 399 +D P +D Q+A + P K EE+++D + WV EL VI + Sbjct: 162 LDDPFDDPP-QLAEMMPDASPEGKPKEEVKDDVRLED-DWVPMDEELGAQELEEVIREKA 219 Query: 398 IEAQPEDLEKAQLVDLPVENVAEPEDLSPVQVVNPIVEN 282 + LE + D+P V P+++ V +NP+ E+ Sbjct: 220 AHSSAVVLES--IGDIPEAEVKPPDNVLFVCKLNPVTED 256 >At5g45830.1 68418.m05637 expressed protein low similarity to tumor-related protein [Nicotiana glauca x Nicotiana langsdorffii] GI:688423 Length = 291 Score = 28.7 bits (61), Expect = 4.6 Identities = 23/87 (26%), Positives = 40/87 (45%), Gaps = 3/87 (3%) Frame = -2 Query: 557 ADLQVAPVNIVDEPVEKTNEEIENDSEVGSISWVEYPAELDEDGNGLVIVDLPIEAQPED 378 A + V V I+DE + T + + I E++ G V+VD ++ Q E Sbjct: 147 AKINVLHVKIIDEEEKMTKKVSSLQEDAADIPIATVAYEMENVGEPNVVVDQALDKQEEA 206 Query: 377 LEK--AQLVDLPVENVAEPED-LSPVQ 306 + + + +L V+ +A+ LSPVQ Sbjct: 207 MARLLVEADNLRVDTLAKILGILSPVQ 233 >At3g57660.1 68416.m06424 DNA-directed RNA polymerase family protein similar to SP|O35134 DNA-directed RNA polymerase I largest subunit (EC 2.7.7.6) (RNA polymerase I 194 kDa subunit) (RPA194) {Mus musculus}; contains InterPro accession IPR000722: RNA polymerase, alpha subunit Length = 1670 Score = 28.7 bits (61), Expect = 4.6 Identities = 25/99 (25%), Positives = 43/99 (43%), Gaps = 3/99 (3%) Frame = -2 Query: 533 NIVDEPVEKTNEEIENDSEVGSISWVEYPAELDEDGNGLVIVDLPIEAQPEDLEKAQLVD 354 N V P+ E +ND S+S + + D+DG G + DL +AQ + ++ +D Sbjct: 1315 NDVTGPI--AGNETDNDD---SVSGKQNEDDGDDDGEGTEVDDLGSDAQKQKKQETDEMD 1369 Query: 353 L---PVENVAEPEDLSPVQVVNPIVENSQSEYPGKRYPD 246 + EP +S V+ EN +E + P+ Sbjct: 1370 YEENSEDETNEPSSISGVEDPEMDSENEDTEVSKEDTPE 1408 >At1g56520.1 68414.m06499 disease resistance protein (TIR-NBS-LRR class), putative domain signature TIR-NBS-LRR exists, suggestive of a disease resistance protein. Length = 897 Score = 28.3 bits (60), Expect = 6.0 Identities = 17/58 (29%), Positives = 32/58 (55%), Gaps = 1/58 (1%) Frame = +3 Query: 600 ISRDIGVLNWSIDVVYEVYRISQDIGVLXWSIEXFYEAYRVQPGN-RHSHWSFDISSK 770 +S++ +WS+D + E+ + +DIG + ++ FYE V P + R+ F I+ K Sbjct: 73 LSKNYASSSWSLDELLEILKCKEDIGQIVMTV--FYE---VDPSDVRNQTGDFGIAFK 125 >At1g55820.1 68414.m06400 hydroxyproline-rich glycoprotein family protein Length = 575 Score = 27.9 bits (59), Expect = 8.0 Identities = 26/116 (22%), Positives = 47/116 (40%), Gaps = 2/116 (1%) Frame = -2 Query: 518 PVEKT-NEEIENDSEVGSISW-VEYPAELDEDGNGLVIVDLPIEAQPEDLEKAQLVDLPV 345 PVE+ EE +++E I++ E P + L + P+ + + + P+ Sbjct: 302 PVEEVPKEEALSNTETHQIAYGQEAPLSVFGLVPSLSAIGQPVNTEAAETQPGNSNSPPI 361 Query: 344 ENVAEPEDLSPVQVVNPIVENSQSEYPGKRYPDATWR*IIYFSSFYLN*NIYIHNF 177 V+ P D S + + +YP +P Y+S +Y+ YIH F Sbjct: 362 SLVSYPPDQSSIAAATQQTNFLRQQYPPNFFPYG------YYSPYYMP-PPYIHQF 410 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,815,008 Number of Sequences: 28952 Number of extensions: 271500 Number of successful extensions: 916 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 880 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 916 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1746037600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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