Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11b10f
(612 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52240.1 68418.m06484 cytochrome b5 domain-containing protein... 91 7e-19
At3g48890.1 68416.m05341 cytochrome b5 domain-containing protein... 90 1e-18
At2g24940.1 68415.m02982 cytochrome b5 domain-containing protein... 85 3e-17
At4g14965.1 68417.m02300 cytochrome b5 domain-containing protein... 74 9e-14
At4g16790.1 68417.m02536 hydroxyproline-rich glycoprotein family... 35 0.049
At5g36060.1 68418.m04344 hypothetical protein 33 0.15
At3g56140.1 68416.m06240 expressed protein At2g40400 - Arabidops... 31 0.60
At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 31 0.60
At1g35080.1 68414.m04347 hypothetical protein 31 0.60
At4g11750.1 68417.m01874 kelch repeat-containing F-box family pr... 30 1.4
At1g76240.1 68414.m08853 expressed protein contains Pfam profile... 29 1.8
At4g15053.1 68417.m02312 hypothetical protein contains Pfam pro... 29 2.4
At5g14920.1 68418.m01750 gibberellin-regulated family protein si... 29 3.2
At5g56890.1 68418.m07099 protein kinase family protein contains ... 28 5.6
At3g53570.3 68416.m05915 protein kinase (AFC1) (AME2) identical ... 28 5.6
At3g53570.2 68416.m05916 protein kinase (AFC1) (AME2) identical ... 28 5.6
At3g53570.1 68416.m05917 protein kinase (AFC1) (AME2) identical ... 28 5.6
At1g77510.1 68414.m09026 protein disulfide isomerase, putative s... 28 5.6
At4g02350.1 68417.m00319 exocyst complex subunit Sec15-like fami... 27 9.8
At2g21235.1 68415.m02522 bZIP protein-related similar to VirE2-i... 27 9.8
>At5g52240.1 68418.m06484 cytochrome b5 domain-containing protein
similar to SP|P70580 Membrane associated progesterone
receptor component 1 {Rattus norvegicus}; contains Pfam
profile PF00173: Heme/Steroid binding domain
Length = 220
Score = 90.6 bits (215), Expect = 7e-19
Identities = 44/97 (45%), Positives = 63/97 (64%), Gaps = 1/97 (1%)
Frame = +1
Query: 307 DMTAVELRQYDGTQEGGRVLMAVNGWIFDVTRGNRFYGPGGPYAVFGGRDATRGLATFSV 486
++T EL+QYDG+ +LMA+ I+DVT+ FYGPGGPYA+F G+DA+R LA S
Sbjct: 74 EITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFAGKDASRALAKMSF 133
Query: 487 TAPEKDYD-DLSDLNSMEMESVREWEEQFRENYDLVG 594
EKD D+S L E++++++WE +F Y VG
Sbjct: 134 --EEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVG 168
>At3g48890.1 68416.m05341 cytochrome b5 domain-containing protein
similar to SP|O00264 Membrane associated progesterone
receptor component (mPR) {Homo sapiens}; contains Pfam
profile PF00173: Heme/Steroid binding domain
Length = 233
Score = 89.8 bits (213), Expect = 1e-18
Identities = 41/98 (41%), Positives = 63/98 (64%)
Frame = +1
Query: 307 DMTAVELRQYDGTQEGGRVLMAVNGWIFDVTRGNRFYGPGGPYAVFGGRDATRGLATFSV 486
++T EL+ YDG+ +LMA+ G I+DV++ FYGPGGPYA+F G+DA+R LA S
Sbjct: 70 EITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 129
Query: 487 TAPEKDYDDLSDLNSMEMESVREWEEQFRENYDLVGRL 600
+ D+S L + E+E++++WE +F Y VG +
Sbjct: 130 EDQDLT-GDISGLGAFELEALQDWEYKFMSKYVKVGTI 166
>At2g24940.1 68415.m02982 cytochrome b5 domain-containing protein
similar to SP|P70580 Membrane associated progesterone
receptor component 1 {Rattus norvegicus}; contains Pfam
profile PF00173: Heme/Steroid binding domain
Length = 100
Score = 85.0 bits (201), Expect = 3e-17
Identities = 40/100 (40%), Positives = 60/100 (60%), Gaps = 1/100 (1%)
Frame = +1
Query: 307 DMTAVELRQYDGTQEGGRVLMAVNGWIFDVTRGNRFYGPGGPYAVFGGRDATRGLATFSV 486
+ TA +L QY+GT E + +A+ G +FDVT G FYG GG Y++F G+DA+R L S
Sbjct: 2 EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61
Query: 487 TAPEKDYD-DLSDLNSMEMESVREWEEQFRENYDLVGRLL 603
E+D L L E+ ++ +WE +F Y +VGR++
Sbjct: 62 N--EEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRVV 99
>At4g14965.1 68417.m02300 cytochrome b5 domain-containing protein
similar to SP|O15173 Membrane associated progesterone
receptor component 2 (Steroid receptor protein DG6)
{Homo sapiens}; contains Pfam profile PF00173:
Heme/Steroid binding domain
Length = 245
Score = 73.7 bits (173), Expect = 9e-14
Identities = 45/134 (33%), Positives = 70/134 (52%)
Frame = +1
Query: 202 FVFVSTFVILYKFYYKYKTHCSKVAPLKKLPKLRKDMTAVELRQYDGTQEGGRVLMAVNG 381
FV V+ V+L Y++ + K+L +A EL Y+GT E +L+ + G
Sbjct: 12 FVGVTFIVVLVSLYFRSSFKSPQHQYQKRL------FSAEELALYNGTDETLPILLGILG 65
Query: 382 WIFDVTRGNRFYGPGGPYAVFGGRDATRGLATFSVTAPEKDYDDLSDLNSMEMESVREWE 561
+FDVT+G YG GG Y F GRDA+R + + T + D L L+S E++S+ +W
Sbjct: 66 SVFDVTKGKFHYGSGGGYNHFAGRDASRAFVSGNFTG-DGLTDSLQGLSSSEVKSIVDWR 124
Query: 562 EQFRENYDLVGRLL 603
+ Y VG+L+
Sbjct: 125 GFYSRTYTPVGKLV 138
>At4g16790.1 68417.m02536 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 473
Score = 34.7 bits (76), Expect = 0.049
Identities = 24/81 (29%), Positives = 39/81 (48%)
Frame = -2
Query: 560 SHSLTDSISIELRSLKSS*SFSGAVTENVASPLVASLPPKTAYGPPGP*NRFPLVTSNIH 381
S S + S S E+ SL S + +T + PL+ +L P +++ P N P + S H
Sbjct: 224 SSSSSSSSSKEVESLPSVKN----LTTVESQPLIKNLTPPSSFSSPRKSNPIPNLASEFH 279
Query: 380 PFTAINTLPPSCVPSYCRSST 318
P PP +P++ SS+
Sbjct: 280 PSPPPPPPPPPPLPAFYNSSS 300
>At5g36060.1 68418.m04344 hypothetical protein
Length = 165
Score = 33.1 bits (72), Expect = 0.15
Identities = 23/70 (32%), Positives = 32/70 (45%)
Frame = +3
Query: 261 LFKGCSLEKTT*IAKRYDRSRTTAVRRDTGGWQSVDGCEWVDIRCD*RESVLRSRWAICC 440
L+KG + E +A+ Y S + GGW +DG W R RES+ R C
Sbjct: 100 LYKGIAYENL--VAECYRSSLWMEPYGEDGGWVQIDGAYWAQPRV--RESIPR-----CS 150
Query: 441 FWRKRCHKRT 470
+ R HKR+
Sbjct: 151 RCKSRAHKRS 160
>At3g56140.1 68416.m06240 expressed protein At2g40400 - Arabidopsis
thaliana, EMBL:AC007020
Length = 745
Score = 31.1 bits (67), Expect = 0.60
Identities = 20/52 (38%), Positives = 23/52 (44%)
Frame = -2
Query: 521 SLKSS*SFSGAVTENVASPLVASLPPKTAYGPPGP*NRFPLVTSNIHPFTAI 366
SL S S S VT S P T PP P N+ +TS I+ TAI
Sbjct: 88 SLASEESSSATVTSPAESAAPPPPPATTTPSPPPPVNKEETITSRIYDATAI 139
>At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar
to SP|P35207 Antiviral protein SKI2 {Saccharomyces
cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain
Length = 1347
Score = 31.1 bits (67), Expect = 0.60
Identities = 23/62 (37%), Positives = 29/62 (46%), Gaps = 2/62 (3%)
Frame = +1
Query: 427 GPYAVFGGRDATRGL-ATFSVTAPEKD-YDDLSDLNSMEMESVREWEEQFRENYDLVGRL 600
G Y GR RGL T +V +D D SDL + + S E QFR Y ++ L
Sbjct: 769 GEYTQMAGRAGRRGLDKTGTVVVMCRDEVPDESDLRRVIVGSATRLESQFRLTYIMILHL 828
Query: 601 LR 606
LR
Sbjct: 829 LR 830
>At1g35080.1 68414.m04347 hypothetical protein
Length = 386
Score = 31.1 bits (67), Expect = 0.60
Identities = 17/47 (36%), Positives = 21/47 (44%), Gaps = 3/47 (6%)
Frame = +3
Query: 261 LFKGCSLEKTT*IAKRYDRSRTTAVRR---DTGGWQSVDGCEWVDIR 392
LFK K T R DRSR++ + GGW +DG W R
Sbjct: 67 LFKAAVYSKETLSCVRLDRSRSSLWMEPYGEDGGWVQIDGAYWAQPR 113
>At4g11750.1 68417.m01874 kelch repeat-containing F-box family
protein contains F-box domain Pfam:PF00646 and Kelch
motif Pfam:PF01344
Length = 386
Score = 29.9 bits (64), Expect = 1.4
Identities = 14/21 (66%), Positives = 14/21 (66%)
Frame = +1
Query: 280 LKKLPKLRKDMTAVELRQYDG 342
LKKLPKL KD T V L Y G
Sbjct: 304 LKKLPKLPKDCTCVRLLGYSG 324
>At1g76240.1 68414.m08853 expressed protein contains Pfam profile
PF03087: Arabidopsis protein of unknown function
Length = 308
Score = 29.5 bits (63), Expect = 1.8
Identities = 18/44 (40%), Positives = 22/44 (50%)
Frame = -2
Query: 599 SRPTRS*FSLNCSSHSLTDSISIELRSLKSS*SFSGAVTENVAS 468
S TRS SL C SH L ++ E+ LKS SF+G S
Sbjct: 28 SHHTRS-ISLPCRSHPLISHVNHEISQLKSWFSFAGETHSRTTS 70
>At4g15053.1 68417.m02312 hypothetical protein contains Pfam
profile PF03080: Arabidopsis proteins of unknown
function
Length = 397
Score = 29.1 bits (62), Expect = 2.4
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = -2
Query: 362 TLPPSCVPSYCRSSTAVISFRNLGSFFKGATFEQCV 255
T P+ V Y + AV +N+G F GAT E C+
Sbjct: 146 TYDPNNVVHYNQHRFAVARTKNIGRRFNGATMELCI 181
>At5g14920.1 68418.m01750 gibberellin-regulated family protein
similar to SP|P46689 Gibberellin-regulated protein 1
precursor {Arabidopsis thaliana}; contains Pfam profile
PF02704: Gibberellin regulated protein
Length = 275
Score = 28.7 bits (61), Expect = 3.2
Identities = 18/50 (36%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
Frame = -2
Query: 485 TENVASPLVASLPPKTAYGPPGP*NRFP-LVTSNIHPFTAINTLPPSCVP 339
T + SP A+ PP A PP P + P L T+ I P T + P +P
Sbjct: 35 TPTLPSPSPATKPPSPALKPPTPSYKPPTLPTTPIKPPTTKPPVKPPTIP 84
>At5g56890.1 68418.m07099 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 1113
Score = 27.9 bits (59), Expect = 5.6
Identities = 18/42 (42%), Positives = 20/42 (47%), Gaps = 2/42 (4%)
Frame = -2
Query: 500 FSGAVTENVASPLVASLP--PKTAYGPPGP*NRFPLVTSNIH 381
FSG VT S V+ +P P A PP P N P SN H
Sbjct: 145 FSGRVTPAPVSSPVSDIPPIPSVALPPPTPSNVPPRNASNNH 186
>At3g53570.3 68416.m05915 protein kinase (AFC1) (AME2) identical to
protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
Length = 453
Score = 27.9 bits (59), Expect = 5.6
Identities = 18/55 (32%), Positives = 26/55 (47%), Gaps = 3/55 (5%)
Frame = -2
Query: 488 VTENVASPLVASLPPKTAYGPP---GP*NRFPLVTSNIHPFTAINTLPPSCVPSY 333
+T + A PL+ PP T + PP GP LV + ++P N LP P +
Sbjct: 26 LTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNFVYPNMFYNGLPRQGSPPW 80
>At3g53570.2 68416.m05916 protein kinase (AFC1) (AME2) identical to
protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
Length = 467
Score = 27.9 bits (59), Expect = 5.6
Identities = 18/55 (32%), Positives = 26/55 (47%), Gaps = 3/55 (5%)
Frame = -2
Query: 488 VTENVASPLVASLPPKTAYGPP---GP*NRFPLVTSNIHPFTAINTLPPSCVPSY 333
+T + A PL+ PP T + PP GP LV + ++P N LP P +
Sbjct: 40 LTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNFVYPNMFYNGLPRQGSPPW 94
>At3g53570.1 68416.m05917 protein kinase (AFC1) (AME2) identical to
protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
Length = 467
Score = 27.9 bits (59), Expect = 5.6
Identities = 18/55 (32%), Positives = 26/55 (47%), Gaps = 3/55 (5%)
Frame = -2
Query: 488 VTENVASPLVASLPPKTAYGPP---GP*NRFPLVTSNIHPFTAINTLPPSCVPSY 333
+T + A PL+ PP T + PP GP LV + ++P N LP P +
Sbjct: 40 LTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNFVYPNMFYNGLPRQGSPPW 94
>At1g77510.1 68414.m09026 protein disulfide isomerase, putative
similar to protein disulfide isomerase precursor
GB:P29828 GI:4704766 [Medicago sativa]; Pfam HMM hit:
PF00085 Thioredoxins
Length = 508
Score = 27.9 bits (59), Expect = 5.6
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +1
Query: 163 ETLKTLFCQPFIYFVFVSTFVILYKFYYKYKTHCSKVAPL 282
E +K + + VF S +L +FY + HC K+AP+
Sbjct: 372 EPVKVVVAESLDDIVFKSGKNVLIEFYAPWCGHCQKLAPI 411
>At4g02350.1 68417.m00319 exocyst complex subunit Sec15-like family
protein contains Pfam profile PF04091: Exocyst complex
subunit Sec15-like
Length = 771
Score = 27.1 bits (57), Expect = 9.8
Identities = 15/38 (39%), Positives = 27/38 (71%), Gaps = 2/38 (5%)
Frame = -2
Query: 557 HSLTD--SISIELRSLKSS*SFSGAVTENVASPLVASL 450
H++ D S+ ++ SLKS+ S S + ++VA+PL++SL
Sbjct: 84 HAVDDLKSLLSDVESLKSALSDSNSKLQSVAAPLLSSL 121
>At2g21235.1 68415.m02522 bZIP protein-related similar to
VirE2-interacting protein VIP1 [Arabidopsis thaliana]
GI:7258340, tbZIP transcription factor [Arabidopsis
thaliana] GI:17065884
Length = 550
Score = 27.1 bits (57), Expect = 9.8
Identities = 17/63 (26%), Positives = 28/63 (44%)
Frame = -2
Query: 455 SLPPKTAYGPPGP*NRFPLVTSNIHPFTAINTLPPSCVPSYCRSSTAVISFRNLGSFFKG 276
SL +T+ PP R+P + + P T P + ++S ++ SF N SF
Sbjct: 27 SLSSRTSLSPPLI--RYPAGSPDFSPGPRCTTQPSPTFSDFTQASPSLTSFNNPASFTPS 84
Query: 275 ATF 267
+F
Sbjct: 85 FSF 87
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,872,717
Number of Sequences: 28952
Number of extensions: 305841
Number of successful extensions: 929
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 863
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 924
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1226538000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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