BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11a19r (644 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g41140.1 68415.m05081 calcium-dependent protein kinase, putat... 29 3.5 At1g35150.1 68414.m04359 hypothetical protein 28 4.6 At5g05560.1 68418.m00604 E3 ubiquitin ligase, putative E3, ubiqu... 28 6.1 >At2g41140.1 68415.m05081 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium/calmodulin-dependent protein kinase CaMK3 [Nicotiana tabacum] gi|16904226|gb|AAL30820 Length = 576 Score = 28.7 bits (61), Expect = 3.5 Identities = 13/52 (25%), Positives = 30/52 (57%) Frame = -1 Query: 161 LFEFTNMNLCMESKDLYYIQILLFVSSTIRLFGIISAKLFEMHYKLIYHVFE 6 +F+F +M C++ K L + + F +S + ++ + + + +E H + Y +FE Sbjct: 469 VFDFVHMISCLQYKKLDFEE---FCASALSVYQLEAMETWEQHARRAYELFE 517 >At1g35150.1 68414.m04359 hypothetical protein Length = 459 Score = 28.3 bits (60), Expect = 4.6 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 3/41 (7%) Frame = -2 Query: 385 KDFTFRKKKYITRVS---DQNIDLRKFNDIIINYIV*FITQ 272 K T R+KK+ VS D+N+DL D INY I Q Sbjct: 386 KKRTIRRKKHFDEVSEKGDENMDLSPEEDFRINYFFTLIDQ 426 >At5g05560.1 68418.m00604 E3 ubiquitin ligase, putative E3, ubiquitin ligase; contains similarity to Apc1/Tsg24 protein, the largest subunit of human anaphase-promoting complex (APC/C) GI:11967711 from [Homo sapiens] Length = 1678 Score = 27.9 bits (59), Expect = 6.1 Identities = 17/45 (37%), Positives = 24/45 (53%), Gaps = 1/45 (2%) Frame = +2 Query: 290 DIIN-YNIIELSQINILVADPGNVFFFSKGKIFNRDFSSICL*LI 421 D++N Y + L++I L A PGN F K +R +CL LI Sbjct: 1275 DLLNSYALYLLNEIKPLSATPGNAFPRGISKFVDRGTLEMCLYLI 1319 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,338,491 Number of Sequences: 28952 Number of extensions: 238064 Number of successful extensions: 472 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 465 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 472 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1334473344 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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