BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11a11f (617 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 29 0.41 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 29 0.54 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 27 1.6 SPBC31F10.13c |hip1|hir1|hira protein Hip1|Schizosaccharomyces p... 27 1.6 SPCC1235.01 ||SPCC320.02c|sequence orphan|Schizosaccharomyces po... 27 2.2 SPAC977.12 |||L-asparaginase |Schizosaccharomyces pombe|chr 1|||... 26 3.8 SPBPB8B6.05c |||L-asparaginase |Schizosaccharomyces pombe|chr 2|... 26 3.8 SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 26 3.8 SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 26 5.0 SPCC1322.03 |||TRP-like ion channel|Schizosaccharomyces pombe|ch... 25 8.8 >SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 29.5 bits (63), Expect = 0.41 Identities = 28/82 (34%), Positives = 37/82 (45%), Gaps = 3/82 (3%) Frame = +2 Query: 275 TATSTP*GIHTTAPTEVPLSSSTDGTTMATPR*TLS---SDPPSLPFKT*MSSLLTGTDL 445 T TSTP T APT +SS TT P + S S+ P T SS T Sbjct: 603 TPTSTPLSNSTVAPTST-FTSSGFNTTSGLPTSSASTPLSNSTVAPTSTFTSSGFNTTSG 661 Query: 446 LIQTTILPPLASRMSVAPSATF 511 L ++ P +S S+ P++TF Sbjct: 662 LPTSSASTP-SSNSSIVPTSTF 682 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.1 bits (62), Expect = 0.54 Identities = 24/83 (28%), Positives = 37/83 (44%), Gaps = 2/83 (2%) Frame = +2 Query: 203 PEAAPIISTGFSPDRTKTIARCL*TATSTP*GIHTTAPT--EVPLSSSTDGTTMATPR*T 376 P A STG S + + T+TS P ++T P VP +S++ +T P T Sbjct: 317 PPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNSTTPVTPTVPPTSTSSTSTPPPPAST 376 Query: 377 LSSDPPSLPFKT*MSSLLTGTDL 445 S+ S P + +S T T + Sbjct: 377 SSTGTSSSPLLSTSTSCTTSTSI 399 Score = 26.6 bits (56), Expect = 2.9 Identities = 27/116 (23%), Positives = 47/116 (40%) Frame = +2 Query: 203 PEAAPIISTGFSPDRTKTIARCL*TATSTP*GIHTTAPTEVPLSSSTDGTTMATPR*TLS 382 P A STG S + + T+TS P ++T P + ++ T + T T S Sbjct: 371 PPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGNSTTPVTPTVPPTSSSTPLTTTNCTTS 430 Query: 383 SDPPSLPFKT*MSSLLTGTDLLIQTTILPPLASRMSVAPSATFWFGSSTMLEAIGT 550 + P T ++S T +T +P ++ ++ P T +ST + T Sbjct: 431 TSVPYT--STPVTSTPLATTNCTTSTSVPYTSTPVTSTPLTTTNCTTSTSIPYTST 484 Score = 25.8 bits (54), Expect = 5.0 Identities = 22/72 (30%), Positives = 32/72 (44%), Gaps = 2/72 (2%) Frame = +2 Query: 299 IHTTAPTEVPLSSSTDGTTMATPR*TLSSDPPSLPFKT*MSSL-LTGTDLLIQT-TILPP 472 + T +P E+ +S T T+ +TP T S+ S T SS L T T T +PP Sbjct: 64 LSTGSPVEITSTSCTTDTSASTPIITESTSSTSSASTTGSSSSPLPSTSTSCTTSTSIPP 123 Query: 473 LASRMSVAPSAT 508 S++ T Sbjct: 124 TGGSSSLSTPIT 135 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 27.5 bits (58), Expect = 1.6 Identities = 24/62 (38%), Positives = 29/62 (46%), Gaps = 9/62 (14%) Frame = -1 Query: 617 PPAWRPALPTT*APKLKPI----RCTW--FQLPPALLMSQTKKLPRARPTS---GTPAAA 465 PPA PA+P AP L P+ R + PP+L S LP + P S G PAA Sbjct: 388 PPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAP 447 Query: 464 VL 459 L Sbjct: 448 PL 449 >SPBC31F10.13c |hip1|hir1|hira protein Hip1|Schizosaccharomyces pombe|chr 2|||Manual Length = 932 Score = 27.5 bits (58), Expect = 1.6 Identities = 18/50 (36%), Positives = 26/50 (52%) Frame = +2 Query: 290 P*GIHTTAPTEVPLSSSTDGTTMATPR*TLSSDPPSLPFKT*MSSLLTGT 439 P + TT PT VP SSST + TP+ + P L +T ++ LT + Sbjct: 401 PSSLSTTDPTLVPQSSSTPKSAQKTPQKLPAFLPNRLTAETVDTNKLTAS 450 >SPCC1235.01 ||SPCC320.02c|sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 658 Score = 27.1 bits (57), Expect = 2.2 Identities = 28/108 (25%), Positives = 39/108 (36%), Gaps = 6/108 (5%) Frame = +2 Query: 200 APEAAPIISTGFSPDRTKTIARCL*TATSTP*GIHTTAPTEVPLSSSTDGTTMATPR*TL 379 A P++ T P T + T T TP TT V + TM TP Sbjct: 89 AAMTTPMVETTTIPTVETTTTPMVETTTITPMVETTTITPMVEAMITLMEETMTTPMEET 148 Query: 380 SSDPPSLPFKT*MSSLLTGTDLLIQTTI------LPPLASRMSVAPSA 505 ++ P T M T T ++ TTI P+ M++ P A Sbjct: 149 TTILPMAAMTTPMEETTTTTPMVETTTIPTVETMTTPMVEAMTILPMA 196 >SPAC977.12 |||L-asparaginase |Schizosaccharomyces pombe|chr 1|||Manual Length = 356 Score = 26.2 bits (55), Expect = 3.8 Identities = 13/45 (28%), Positives = 22/45 (48%) Frame = -3 Query: 300 IPHGVDVAVHKHLAIVLVLSGEKPVLIIGAASGAQ*FIVDGFMQV 166 I HG D + + L +S KP++++GA + DG M + Sbjct: 122 ITHGTDSLEETAMFLDLTISTAKPIVVVGAMRPSTAIGADGPMNL 166 >SPBPB8B6.05c |||L-asparaginase |Schizosaccharomyces pombe|chr 2|||Manual Length = 356 Score = 26.2 bits (55), Expect = 3.8 Identities = 13/45 (28%), Positives = 22/45 (48%) Frame = -3 Query: 300 IPHGVDVAVHKHLAIVLVLSGEKPVLIIGAASGAQ*FIVDGFMQV 166 I HG D + + L +S KP++++GA + DG M + Sbjct: 122 ITHGTDSLEETAMFLDLTISTAKPIVVVGAMRPSTAIGADGPMNL 166 >SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 471 Score = 26.2 bits (55), Expect = 3.8 Identities = 17/56 (30%), Positives = 24/56 (42%) Frame = +2 Query: 287 TP*GIHTTAPTEVPLSSSTDGTTMATPR*TLSSDPPSLPFKT*MSSLLTGTDLLIQ 454 TP +++ P E P S S TM P S+PF T + L G ++ Q Sbjct: 401 TPSFFNSSQPIETPSSYSHLSDTMLVPANMAGIPSVSIPFGTLNNGLPMGIQIMAQ 456 >SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 25.8 bits (54), Expect = 5.0 Identities = 27/107 (25%), Positives = 47/107 (43%), Gaps = 7/107 (6%) Frame = +2 Query: 209 AAPIISTGFSPDRTKTIARCL*TATST-----P*GIHTTAPTEVPLSSSTDGTTMATPR* 373 +A + +T FS T+T + + T P H + T VP ++S + TT P Sbjct: 41 SANVTTTSFSSASTETSTHSVTSTNITSIVPPPSTSHNSTTTTVPPTTSMNTTTTVPPTT 100 Query: 374 TLSSDPPSLPFKT*MSSLLTGTDLLIQTTILPPL--ASRMSVAPSAT 508 +L++ + P T ++S TT++PP + +V P T Sbjct: 101 SLNTTTTTAPPTTHVNST---------TTVVPPTTHVNTTTVVPPTT 138 >SPCC1322.03 |||TRP-like ion channel|Schizosaccharomyces pombe|chr 3|||Manual Length = 862 Score = 25.0 bits (52), Expect = 8.8 Identities = 10/31 (32%), Positives = 17/31 (54%) Frame = +2 Query: 443 LLIQTTILPPLASRMSVAPSATFWFGSSTML 535 L +QT +PP+ S+ PS SS+++ Sbjct: 656 LFVQTNCIPPVTQASSLVPSKNNTASSSSLM 686 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,649,368 Number of Sequences: 5004 Number of extensions: 56207 Number of successful extensions: 182 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 165 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 182 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 271646730 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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