Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11a11f
(617 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g15860.2 68418.m01856 expressed protein 31 0.81
At5g15860.1 68418.m01855 expressed protein 31 0.81
At3g02410.1 68416.m00228 hypothetical protein weak similarity to... 30 1.1
At2g31710.1 68415.m03870 expressed protein 30 1.1
At1g14020.1 68414.m01656 expressed protein contains Pfam PF03138... 30 1.4
At2g03280.1 68415.m00282 expressed protein contains Pfam PF03138... 29 2.5
At3g19020.1 68416.m02415 leucine-rich repeat family protein / ex... 29 3.3
At1g25300.1 68414.m03140 octicosapeptide/Phox/Bem1p (PB1) domain... 28 4.3
At1g71440.1 68414.m08253 tubulin folding cofactor E / Pfifferlin... 27 7.5
At1g57570.1 68414.m06533 jacalin lectin family protein similar t... 27 7.5
At5g56160.1 68418.m07006 SEC14 cytosolic factor family protein /... 27 10.0
At5g46820.1 68418.m05768 hypothetical protein contains similarit... 27 10.0
At2g31270.1 68415.m03818 hydroxyproline-rich glycoprotein family... 27 10.0
At1g70960.1 68414.m08186 F-box family protein contains Pfam PF00... 27 10.0
At1g22460.1 68414.m02807 expressed protein similar to axi 1 [Nic... 27 10.0
>At5g15860.2 68418.m01856 expressed protein
Length = 299
Score = 30.7 bits (66), Expect = 0.81
Identities = 21/68 (30%), Positives = 37/68 (54%)
Frame = +1
Query: 397 LAVQDVNVIVVDWHRLANSNYNTAAAGVPDVGRALGNFLVWLINNAGGNWNQVHLIGFSL 576
LA +D+ V +D+ N T + V D + + +F+ I+ GG+ N+++L+G S
Sbjct: 181 LAERDIIVACLDYR---NFPQGTISDMVTDASQGI-SFVCNNISAFGGDPNRIYLMGQSA 236
Query: 577 GAHVVGNA 600
GAH+ A
Sbjct: 237 GAHIAACA 244
>At5g15860.1 68418.m01855 expressed protein
Length = 427
Score = 30.7 bits (66), Expect = 0.81
Identities = 21/68 (30%), Positives = 37/68 (54%)
Frame = +1
Query: 397 LAVQDVNVIVVDWHRLANSNYNTAAAGVPDVGRALGNFLVWLINNAGGNWNQVHLIGFSL 576
LA +D+ V +D+ N T + V D + + +F+ I+ GG+ N+++L+G S
Sbjct: 181 LAERDIIVACLDYR---NFPQGTISDMVTDASQGI-SFVCNNISAFGGDPNRIYLMGQSA 236
Query: 577 GAHVVGNA 600
GAH+ A
Sbjct: 237 GAHIAACA 244
>At3g02410.1 68416.m00228 hypothetical protein weak similarity to
kynurenine formamidase [Mus musculus] GI:21552719
Length = 422
Score = 30.3 bits (65), Expect = 1.1
Identities = 20/64 (31%), Positives = 36/64 (56%)
Frame = +1
Query: 397 LAVQDVNVIVVDWHRLANSNYNTAAAGVPDVGRALGNFLVWLINNAGGNWNQVHLIGFSL 576
LA +D+ V +D+ N T + V D + + +F+ I+ GG+ N+++L+G S
Sbjct: 176 LAERDIIVACLDYR---NFPQGTISDMVSDAAQGI-SFVCNNISAFGGDPNRIYLMGQSA 231
Query: 577 GAHV 588
GAH+
Sbjct: 232 GAHI 235
>At2g31710.1 68415.m03870 expressed protein
Length = 105
Score = 30.3 bits (65), Expect = 1.1
Identities = 18/46 (39%), Positives = 30/46 (65%), Gaps = 6/46 (13%)
Frame = +1
Query: 328 VVIVHGWNNN---GNTQMNP---LIRSAFLAVQDVNVIVVDWHRLA 447
+ I++G+NN+ G+T ++P + S FLAV VNV++V + LA
Sbjct: 20 IAILYGFNNDLLPGSTTLSPHSLTLLSGFLAVVSVNVVIVFYICLA 65
>At1g14020.1 68414.m01656 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'growth regulator protein -related' based on similarity
to axi 1 protein (GB:X80301) (GI:559920) from [Nicotiana
tabacum], which, due to scienitific fraud was retracted.
Retraction in: Schell J. EMBO J 1999 May 17;18(10):2908.
PMID:10400497.
Length = 499
Score = 29.9 bits (64), Expect = 1.4
Identities = 18/52 (34%), Positives = 29/52 (55%), Gaps = 5/52 (9%)
Frame = +1
Query: 34 ALAGLVTLCSGVAIPTIPGDNSHYVEGESRYIW-----MPDGEGVPQLVDLH 174
AL +V++ S IPT G+ + VEG RY+ + D + + +L+DLH
Sbjct: 382 ALDFMVSVASNTFIPTYDGNMAKVVEGHRRYLGYKKTILLDRKRLVELLDLH 433
>At2g03280.1 68415.m00282 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as ' axi
1 protein from Nicotiana tabacum -related' based on
similarity to axi 1 protein (GB:X80301) (GI:559920) from
[Nicotiana tabacum], which, due to scienitific fraud was
retracted. Retraction in: Schell J. EMBO J 1999 May
17;18(10):2908. PMID:10400497.
Length = 481
Score = 29.1 bits (62), Expect = 2.5
Identities = 18/52 (34%), Positives = 29/52 (55%), Gaps = 5/52 (9%)
Frame = +1
Query: 34 ALAGLVTLCSGVAIPTIPGDNSHYVEGESRYIW-----MPDGEGVPQLVDLH 174
AL +V++ S IPT G+ + VEG RY+ + D + + +L+DLH
Sbjct: 379 ALDFIVSVASNTFIPTYYGNMAKVVEGHRRYLGFKKTILLDRKRLVELLDLH 430
>At3g19020.1 68416.m02415 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 956
Score = 28.7 bits (61), Expect = 3.3
Identities = 13/44 (29%), Positives = 22/44 (50%)
Frame = +3
Query: 246 EPKQSPGACERQHQLREEFTLPRQPRYRCHRPRMEQQWQHPDEP 377
+PK P + +Q ++E P QP+ + P+ E Q P +P
Sbjct: 477 QPKPKPES-PKQESPKQEAPKPEQPKPKPESPKQESSKQEPPKP 519
>At1g25300.1 68414.m03140 octicosapeptide/Phox/Bem1p (PB1)
domain-containing protein similar to unknown protein
GI:4262226 from [Arabidopsis thaliana] contains Pfam
profile PF00564: PB1 domain
Length = 272
Score = 28.3 bits (60), Expect = 4.3
Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 2/54 (3%)
Frame = +1
Query: 55 LCS--GVAIPTIPGDNSHYVEGESRYIWMPDGEGVPQLVDLHEPIDYELLGSRS 210
LCS G +P P + HY+ GE+R + +P G +D + + +LL RS
Sbjct: 14 LCSYGGRIMPLPPEKSLHYIGGETRLVIVPRG---ISFLDFFKLLSDKLLSGRS 64
>At1g71440.1 68414.m08253 tubulin folding cofactor E / Pfifferling
(PFI) almost identical to tubulin folding cofactor E
(Pfifferling; PFI) GI:20514267 from [Arabidopsis
thaliana]; identical to cDNA tubulin folding cofactor E,
GI:20514266
Length = 531
Score = 27.5 bits (58), Expect = 7.5
Identities = 16/54 (29%), Positives = 31/54 (57%), Gaps = 4/54 (7%)
Frame = +1
Query: 448 NSNYNTAAAGVPDVGRALGNFLVWLINNAGGNWNQVHLIGFSLGA----HVVGN 597
N + N+ ++ + + + L N V ++NN+G +W QV ++ SL H++GN
Sbjct: 192 NLSCNSLSSDIKSLPQ-LKNIRVLVLNNSGLSWTQVEILRRSLPGIEELHLMGN 244
>At1g57570.1 68414.m06533 jacalin lectin family protein similar to
myrosinase-binding protein homolog [Arabidopsis
thaliana] GI:2997767; contains Pfam profile PF01419
jacalin-like lectin domain
Length = 614
Score = 27.5 bits (58), Expect = 7.5
Identities = 15/32 (46%), Positives = 19/32 (59%), Gaps = 2/32 (6%)
Frame = +1
Query: 265 VLVNGNINSVR--NSHYRANRGTVVIVHGWNN 354
V V+ N+ +VR HY NR VV HGWN+
Sbjct: 342 VYVSSNVMNVRYIKFHYY-NRAVVVRQHGWNS 372
>At5g56160.1 68418.m07006 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
similar to phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus] and SEC14 cytosolic
factor (SP:P45816) [Candida lipolytica]
Length = 592
Score = 27.1 bits (57), Expect = 10.0
Identities = 23/69 (33%), Positives = 33/69 (47%)
Frame = +2
Query: 305 TTAPTEVPLSSSTDGTTMATPR*TLSSDPPSLPFKT*MSSLLTGTDLLIQTTILPPLASR 484
TTAP V ST T +P+ TL +P + SS TG+ + T I+PPL+ +
Sbjct: 359 TTAPLHVRDYDST--TCTISPKETLKEEPEPEEY---YSS--TGSRSSMHTCIVPPLSDK 411
Query: 485 MSVAPSATF 511
S + F
Sbjct: 412 ASTSDGDKF 420
>At5g46820.1 68418.m05768 hypothetical protein contains similarity
to carboxyl-terminal proteinase contains Pfam profile
PF03080: Arabidopsis proteins of unknown function
Length = 352
Score = 27.1 bits (57), Expect = 10.0
Identities = 10/29 (34%), Positives = 16/29 (55%)
Frame = +3
Query: 309 PRQPRYRCHRPRMEQQWQHPDEPSHQIRL 395
P++P Y C + QHP +HQI++
Sbjct: 11 PQEPEYECVDIYKQPSLQHPKLQNHQIQM 39
>At2g31270.1 68415.m03818 hydroxyproline-rich glycoprotein family
protein
Length = 571
Score = 27.1 bits (57), Expect = 10.0
Identities = 12/45 (26%), Positives = 26/45 (57%)
Frame = +2
Query: 374 TLSSDPPSLPFKT*MSSLLTGTDLLIQTTILPPLASRMSVAPSAT 508
+L + S+P SS +T T + +++ P +S++++AP+ T
Sbjct: 263 SLMEEMASIPASKLFSSPITSTPVKTTSSLAKPTSSQINIAPTPT 307
>At1g70960.1 68414.m08186 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640: F-box
protein interaction domain
Length = 369
Score = 27.1 bits (57), Expect = 10.0
Identities = 20/83 (24%), Positives = 38/83 (45%), Gaps = 2/83 (2%)
Frame = +1
Query: 319 RGTVVIVHGWNNNGNTQ-MNPLIRSAFLAVQDVNVIVVDWHRLANSNYNTAAAG-VPDVG 492
+G V+ +NNG + M+ + S +V ++ V + + RL N + A + D
Sbjct: 210 KGGVLYYGAKSNNGKSVVMSFNVNSEDFSVIELEVEISPYWRLVNYKGDIALMNNIEDSL 269
Query: 493 RALGNFLVWLINNAGGNWNQVHL 561
F +W+ N GNW++ +
Sbjct: 270 YHSREFEMWVRNEVTGNWDRTSI 292
>At1g22460.1 68414.m02807 expressed protein similar to axi 1
[Nicotiana tabacum] GI:559921; contains Pfam profile
PF03138: Plant protein family
Length = 565
Score = 27.1 bits (57), Expect = 10.0
Identities = 17/50 (34%), Positives = 28/50 (56%), Gaps = 5/50 (10%)
Frame = +1
Query: 34 ALAGLVTLCSGVAIPTIPGDNSHYVEGESRYIW-----MPDGEGVPQLVD 168
AL +V++ S V IP+ G+ + VEG R++ PD + + +LVD
Sbjct: 448 ALDYIVSIESDVFIPSYSGNMARAVEGHRRFLGHRKTISPDRKAIVRLVD 497
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,189,432
Number of Sequences: 28952
Number of extensions: 312990
Number of successful extensions: 875
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 837
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 875
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1246162608
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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