BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV11a05r (662 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3H8.10 |spo20|sec14|sec14 cytosolic factor family Sec14|Schi... 58 1e-09 SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces ... 31 0.20 SPAC1952.13 |ned1||lipin|Schizosaccharomyces pombe|chr 1|||Manual 29 0.79 SPBC15D4.04 |gpt2|gpt, alg7|UDP-N-acetylglucosamine--dolichyl-ph... 28 1.4 SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 27 3.2 SPAC12B10.11 |exg2||glucan 1,3-beta-glucosidase Exg2|Schizosacch... 27 3.2 SPAC343.15 |||tRNA isopentenyltransferase|Schizosaccharomyces po... 26 4.2 SPAC11D3.05 |||membrane transporter|Schizosaccharomyces pombe|ch... 26 5.6 SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 5.6 SPAC17A2.09c |csx1||RNA-binding protein Csx1|Schizosaccharomyces... 26 5.6 SPBC1683.13c |||transcription factor |Schizosaccharomyces pombe|... 26 5.6 SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces ... 25 9.7 SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|c... 25 9.7 SPCC1840.05c |||phosphomannomutase |Schizosaccharomyces pombe|ch... 25 9.7 SPAC222.05c |mss1||COX RNA-associated protein|Schizosaccharomyce... 25 9.7 SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation fac... 25 9.7 >SPAC3H8.10 |spo20|sec14|sec14 cytosolic factor family Sec14|Schizosaccharomyces pombe|chr 1|||Manual Length = 286 Score = 57.6 bits (133), Expect = 1e-09 Identities = 30/94 (31%), Positives = 52/94 (55%), Gaps = 1/94 (1%) Frame = -3 Query: 468 SILDLDTVKIGHLLQMTPMFLKKMVVASQDALPVRLKGTHYLNTPTGFETVFNAVKALLN 289 +I+DL V I + + ++++ SQD P R+ + +N P GF + FN +K L+ Sbjct: 170 TIMDLKGVGITSIHSVYS-YIRQASSISQDYYPERMGKFYVINAPWGFSSAFNLIKGFLD 228 Query: 288 EKNKKRLYVHNQNYEE-MYKYIPKDILPVEYGGN 190 E K++++ NY+ + + IP D LP + GGN Sbjct: 229 EATVKKIHILGSNYKSALLEQIPADNLPAKLGGN 262 >SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 1|||Manual Length = 388 Score = 30.7 bits (66), Expect = 0.20 Identities = 15/59 (25%), Positives = 30/59 (50%) Frame = -3 Query: 339 TPTGFETVFNAVKALLNEKNKKRLYVHNQNYEEMYKYIPKDILPVEYGGNGGSIEEIID 163 +P F +V+N +K + + ++ V Q+ ++ KYI ++P GG I + I+ Sbjct: 244 SPWIFRSVWNIIKGWIKPEIAAKI-VFTQSANDLEKYIDYSVIPTSLGGGNKKIFQYIE 301 >SPAC1952.13 |ned1||lipin|Schizosaccharomyces pombe|chr 1|||Manual Length = 656 Score = 28.7 bits (61), Expect = 0.79 Identities = 18/68 (26%), Positives = 29/68 (42%), Gaps = 1/68 (1%) Frame = -3 Query: 261 HNQNYEEMYKYIPKDILPVEYGGNGG-SIEEIIDYWKKKVKEYSDWLEEDDQYGTDESKR 85 H +Y M + + P+E S +++W+ + E SD EE+D SK Sbjct: 544 HRSSYVYMNELVDHFFPPIEVSTRDEVSSFTDVNFWRSPLLELSD--EEEDDTNKSTSKS 601 Query: 84 PGKPKTAE 61 P PK + Sbjct: 602 PKTPKNTK 609 >SPBC15D4.04 |gpt2|gpt, alg7|UDP-N-acetylglucosamine--dolichyl-phosphateN- acetylglucosaminephosphotransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 27.9 bits (59), Expect = 1.4 Identities = 15/52 (28%), Positives = 25/52 (48%) Frame = +1 Query: 49 PEHILSGLRFPWSLGLVRTILIIFFQPVTVFLYFFLPVIYNFFNRTTVPAIF 204 P + G F + G+V ++ I + FF+P I+NF +VP +F Sbjct: 279 PSRVFVGDTFCYFAGMVMAVVGILGHFSKTLMLFFIPQIFNF--ALSVPQLF 328 >SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosaccharomyces pombe|chr 2|||Manual Length = 2335 Score = 26.6 bits (56), Expect = 3.2 Identities = 11/31 (35%), Positives = 17/31 (54%) Frame = +1 Query: 112 IIFFQPVTVFLYFFLPVIYNFFNRTTVPAIF 204 +IF + +++ LPVI F R T+P F Sbjct: 956 VIFGSNIEEYMHLVLPVIIRSFERDTIPLGF 986 >SPAC12B10.11 |exg2||glucan 1,3-beta-glucosidase Exg2|Schizosaccharomyces pombe|chr 1|||Manual Length = 570 Score = 26.6 bits (56), Expect = 3.2 Identities = 12/25 (48%), Positives = 17/25 (68%) Frame = +1 Query: 94 LVRTILIIFFQPVTVFLYFFLPVIY 168 L+ TIL+ FF + VFL LP+I+ Sbjct: 34 LLITILLTFFVSLCVFLSIILPLIF 58 >SPAC343.15 |||tRNA isopentenyltransferase|Schizosaccharomyces pombe|chr 1|||Manual Length = 434 Score = 26.2 bits (55), Expect = 4.2 Identities = 13/42 (30%), Positives = 22/42 (52%), Gaps = 1/42 (2%) Frame = -3 Query: 393 VASQDALPVRLKGTHYLNTPTGFE-TVFNAVKALLNEKNKKR 271 + SQ +P+ + GTHY FE T +A+ L N+ + + Sbjct: 88 IHSQGKIPIVVGGTHYYLQSLLFEDTTLSAIDKLTNDSSPSK 129 >SPAC11D3.05 |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 546 Score = 25.8 bits (54), Expect = 5.6 Identities = 11/37 (29%), Positives = 20/37 (54%), Gaps = 2/37 (5%) Frame = +1 Query: 100 RTILIIFFQPVTVFLYFFLPVIY--NFFNRTTVPAIF 204 + + +IF +P+ V +L V+Y N+ N P +F Sbjct: 328 QAVSLIFTEPIVVCFTLYLTVVYIINYINFEGYPIVF 364 >SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Schizosaccharomyces pombe|chr 1|||Manual Length = 1995 Score = 25.8 bits (54), Expect = 5.6 Identities = 22/65 (33%), Positives = 31/65 (47%), Gaps = 4/65 (6%) Frame = -3 Query: 432 LLQMTPMFLKKMVVASQDALP-VRLKGTHYLNTPTGFE---TVFNAVKALLNEKNKKRLY 265 +L TP K+M ++S DA+ + K N E TV A L +E N+ + Sbjct: 272 VLNTTP---KEMQISSLDAVDKLEDKPVSNDNIKANIESSTTVDRASSQLESENNESTFF 328 Query: 264 VHNQN 250 VHNQN Sbjct: 329 VHNQN 333 >SPAC17A2.09c |csx1||RNA-binding protein Csx1|Schizosaccharomyces pombe|chr 1|||Manual Length = 632 Score = 25.8 bits (54), Expect = 5.6 Identities = 29/90 (32%), Positives = 36/90 (40%), Gaps = 5/90 (5%) Frame = +3 Query: 231 ICTSLHNSGCGRRGVSYFFRSVM--LL--QH*KQFRILSEYLNNGYL*VE-LVMRPEKQP 395 I +L SG G +S F S L+ Q Q +L NG V+ V PE Sbjct: 465 ISPTLSGSGSGLTPLSSHFPSAATGLVGGQVYPQSSVLQSSKINGSAKVQPSVKLPEWLQ 524 Query: 396 PFFSRTLESFVINDLFLQYLNLKLTKCRQP 485 PF SF DL + +LKL QP Sbjct: 525 PFSGNNHNSFATQDLLTRVSSLKLVDDEQP 554 >SPBC1683.13c |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 618 Score = 25.8 bits (54), Expect = 5.6 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = -2 Query: 334 DRIRNCFQCCKSITERKK*ETPLR 263 DR+ CFQC KS ++ E P+R Sbjct: 30 DRLHPCFQCVKSNSQCFYPEDPIR 53 >SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 1|||Manual Length = 444 Score = 25.0 bits (52), Expect = 9.7 Identities = 18/72 (25%), Positives = 34/72 (47%) Frame = -3 Query: 408 LKKMVVASQDALPVRLKGTHYLNTPTGFETVFNAVKALLNEKNKKRLYVHNQNYEEMYKY 229 LK M+ + P L P F+ V++ +K+ L+ ++ +NY ++ +Y Sbjct: 254 LKFMIKCFEAHYPECLGECIVHKAPWLFQGVWSIIKSWLDPVVVSKVKF-TRNYRDLQQY 312 Query: 228 IPKDILPVEYGG 193 I D + E+GG Sbjct: 313 INPDNILKEFGG 324 >SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1717 Score = 25.0 bits (52), Expect = 9.7 Identities = 13/49 (26%), Positives = 19/49 (38%), Gaps = 2/49 (4%) Frame = -3 Query: 159 WKKKVKEYSDWLEEDDQYGTDESK--RPGKPKTAEDMFGVEGSFRQLEF 19 WK+K++EY W +Q K + K E G +EF Sbjct: 1128 WKEKMREYKKWQSTANQRAKQMEKFEKSATEKQKEQALKEAGDISWIEF 1176 >SPCC1840.05c |||phosphomannomutase |Schizosaccharomyces pombe|chr 3|||Manual Length = 587 Score = 25.0 bits (52), Expect = 9.7 Identities = 20/59 (33%), Positives = 31/59 (52%) Frame = -3 Query: 366 RLKGTHYLNTPTGFETVFNAVKALLNEKNKKRLYVHNQNYEEMYKYIPKDILPVEYGGN 190 +++G H++ T TGF+ + N KAL EK K + YEE Y+ I+ + G N Sbjct: 369 KVEGFHHVETLTGFKWLGN--KALELEKQGKFI---GLAYEEALGYMVGSIVRDKDGVN 422 >SPAC222.05c |mss1||COX RNA-associated protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 496 Score = 25.0 bits (52), Expect = 9.7 Identities = 11/22 (50%), Positives = 15/22 (68%) Frame = -3 Query: 186 GSIEEIIDYWKKKVKEYSDWLE 121 GSI +I W+KK+ EY +LE Sbjct: 172 GSIYDICFRWRKKLIEYRAFLE 193 >SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation factor CPSF-73 |Schizosaccharomyces pombe|chr 2|||Manual Length = 797 Score = 25.0 bits (52), Expect = 9.7 Identities = 16/57 (28%), Positives = 26/57 (45%) Frame = -3 Query: 627 PKTATSDSPRVSIIIPGRYDADKYNISDVMAVSLVLQKIIMMEDDNAMVAGILSILD 457 P T +D+ + IP R D+ I VM+ L +++ D + V + ILD Sbjct: 187 PNTLITDANNSLVSIPSRKKRDEAFIESVMSSLLKGGTVLLPVDAASRVLELCCILD 243 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,900,844 Number of Sequences: 5004 Number of extensions: 65962 Number of successful extensions: 211 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 202 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 210 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 301829700 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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