Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11a05r
(662 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g30690.1 68414.m03752 SEC14 cytosolic factor family protein /... 47 1e-05
At3g51670.1 68416.m05666 SEC14 cytosolic factor family protein /... 38 0.006
At1g72160.1 68414.m08343 SEC14 cytosolic factor family protein /... 35 0.042
At4g09160.1 68417.m01517 SEC14 cytosolic factor family protein /... 35 0.056
At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /... 35 0.056
At5g47510.1 68418.m05866 SEC14 cytosolic factor family protein /... 33 0.17
At1g55840.1 68414.m06404 SEC14 cytosolic factor (SEC14) / phosph... 33 0.17
At1g75170.1 68414.m08731 SEC14 cytosolic factor family protein /... 31 0.68
At1g09890.1 68414.m01113 expressed protein ; expression supporte... 31 0.90
At2g36690.1 68415.m04501 oxidoreductase, 2OG-Fe(II) oxygenase fa... 29 2.1
At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein /... 29 2.1
At5g64070.1 68418.m08046 phosphatidylinositol 4-kinase (PI4K) ne... 29 3.6
At4g37900.1 68417.m05360 glycine-rich protein 29 3.6
At3g10340.1 68416.m01240 phenylalanine ammonia-lyase, putative s... 28 4.8
At5g51670.1 68418.m06406 expressed protein contains Pfam domain ... 28 6.4
At5g22990.1 68418.m02687 zinc finger (C2H2 type) family protein ... 28 6.4
At3g48500.1 68416.m05294 expressed protein 27 8.4
At2g41770.1 68415.m05163 expressed protein contains Pfam domain ... 27 8.4
At2g19880.1 68415.m02324 ceramide glucosyltransferase, putative ... 27 8.4
At1g76460.1 68414.m08893 RNA recognition motif (RRM)-containing ... 27 8.4
>At1g30690.1 68414.m03752 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to cytosolic factor
(Phosphatidylinositol/phosphatidylcholine transfer
protein) (PI/PCTP) (SP:P24280) [Saccharomyces
cerevisiae]; ESTs gb|T76582, gb|N06574 and gb|Z25700
come from this gene
Length = 540
Score = 47.2 bits (107), Expect = 1e-05
Identities = 29/104 (27%), Positives = 50/104 (48%), Gaps = 4/104 (3%)
Frame = -3
Query: 492 NAMVAGILSILDLDTVKIGHLLQMTPMF--LKKMVVASQDALPVRLKGTHYLNTPTGFET 319
N G+ S+L + +K + T ++ +KK++ QD P + ++N P F
Sbjct: 319 NLKPGGVTSLLQIHDLKNAPGVSRTEIWVGIKKVIETLQDNYPEFVSRNIFINVPFWFYA 378
Query: 318 VFNAVKALLNEKNKKRLYVHN--QNYEEMYKYIPKDILPVEYGG 193
+ + L ++ K + V + E + KYIP D LPV+YGG
Sbjct: 379 MRAVLSPFLTQRTKSKFVVARPAKVRETLLKYIPADELPVQYGG 422
>At3g51670.1 68416.m05666 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
polyphosphoinositide binding protein Ssh2p (GI:2739046)
{Glycine max};; contains Pfam PF00650 : CRAL/TRIO
domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus
Length = 409
Score = 37.9 bits (84), Expect = 0.006
Identities = 17/66 (25%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Frame = -3
Query: 384 QDALPVRLKGTHYLNTPTGFETVFNAVKALLNEKNKKRLYVHNQNY--EEMYKYIPKDIL 211
QD P + ++N P F +++ L ++ K + + + E +YK+I + +
Sbjct: 223 QDNYPELVATKIFINVPWYFSVIYSMFSPFLTQRTKSKFVMSKEGNAAETLYKFIRPEDI 282
Query: 210 PVEYGG 193
PV+YGG
Sbjct: 283 PVQYGG 288
>At1g72160.1 68414.m08343 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
GI:807956 from [Saccharomyces cerevisiae]similar to
polyphosphoinositide binding protein Ssh2p (GI:2739046)
{Glycine max}; contains Pfam PF00650 : CRAL/TRIO domain;
contains Pfam PF03765 : CRAL/TRIO, N-terminus
Length = 490
Score = 35.1 bits (77), Expect = 0.042
Identities = 18/73 (24%), Positives = 37/73 (50%), Gaps = 2/73 (2%)
Frame = -3
Query: 405 KKMVVASQDALPVRLKGTHYLNTPTGFETVFNAVKALLNEKNKKRLYVH--NQNYEEMYK 232
K+ V QD P + ++N P + + + + ++K +L +++ E ++K
Sbjct: 299 KQAVELLQDNYPEFVFKQAFINVPWWYLVFYTVIGPFMTPRSKSKLVFAGPSRSAETLFK 358
Query: 231 YIPKDILPVEYGG 193
YI + +PV+YGG
Sbjct: 359 YISPEQVPVQYGG 371
>At4g09160.1 68417.m01517 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
polyphosphoinositide binding protein Ssh1p (GI:2739044)
{Glycine max}; similar to polyphosphoinositide binding
protein Ssh2, Glycine max, gb:T05953; contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus
Length = 668
Score = 34.7 bits (76), Expect = 0.056
Identities = 16/66 (24%), Positives = 35/66 (53%), Gaps = 2/66 (3%)
Frame = -3
Query: 384 QDALPVRLKGTHYLNTPTGFETVFNAVKALLNEKNKKRLYVH--NQNYEEMYKYIPKDIL 211
QD P + ++N P + + + +++++K +L +++ E + KYI + +
Sbjct: 481 QDNYPEFVSKQIFINVPWWYLAFYRIISPFMSQRSKSKLVFAGPSRSAETLLKYISPEHV 540
Query: 210 PVEYGG 193
PV+YGG
Sbjct: 541 PVQYGG 546
>At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to SEC14-like protein 2
(Alpha-tocopherol associated protein) (TAP) (bTAP)
(Fragment) (SP:P58875) {Bos taurus}
Length = 683
Score = 34.7 bits (76), Expect = 0.056
Identities = 17/76 (22%), Positives = 40/76 (52%), Gaps = 3/76 (3%)
Frame = -3
Query: 411 FLKKMVVASQDALPVRLKGTHYLNTPTGFETVFNAVKALLNE---KNKKRLYVHNQNYEE 241
F+K+ V +D P + ++N P + + +++ ++K L +++ E
Sbjct: 495 FIKRAVKQFEDNYPEFVAKELFINVPWWYIPYYKTFGSIITSPRTRSKMVLSGPSKSAET 554
Query: 240 MYKYIPKDILPVEYGG 193
++KY+ +++PV+YGG
Sbjct: 555 IFKYVAPEVVPVKYGG 570
>At5g47510.1 68418.m05866 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus], SEC14 cytosolic factor
(Phosphatidylinositol/phosphatidylcholine transfer
protein) (PI/PCTP) (SP:P24859) [Kluyveromyces lactis]
and to SEC14 cytosolic factor (SP:P53989) [Candida
glabrata]
Length = 376
Score = 33.1 bits (72), Expect = 0.17
Identities = 23/99 (23%), Positives = 43/99 (43%), Gaps = 1/99 (1%)
Frame = -3
Query: 483 VAGILSILDLDTVKIGHLLQMTPMFLKKMVVASQDALPVRLKGTHYLNTPTGFETVFNAV 304
V+ +ILD+ V + + + ++ + P L +N +GF ++ A+
Sbjct: 163 VSSTTTILDVSGVGMSNFSKPARSLFMEIQKIDSNYYPETLHRLFVVNASSGFRMLWLAL 222
Query: 303 KALLNEKNKKRLYVHNQNY-EEMYKYIPKDILPVEYGGN 190
K L+ + ++ V NY E+ + I LP GGN
Sbjct: 223 KTFLDARTLAKVQVLGPNYLGELLEAIEPSNLPTFLGGN 261
>At1g55840.1 68414.m06404 SEC14 cytosolic factor (SEC14) /
phosphoglyceride transfer protein similar to
polyphosphoinositide binding protein SEC14 homolog Ssh1p
(GB:AAB94598) [Glycine max]; identified in Eur J Biochem
1998 Dec 1;258(2):402-10 as AtSEC14, characterized by
functional complementation in S. cerevisiae.
Length = 325
Score = 33.1 bits (72), Expect = 0.17
Identities = 25/91 (27%), Positives = 44/91 (48%), Gaps = 3/91 (3%)
Frame = -3
Query: 471 LSILDLDTVKIGHLLQMTPMFLKKMVVASQDAL--PVRLKGTHYLNTPTGFETVFNAVKA 298
L ILD+ +K+ L Q+ M + + D L P + + + +N P F + +K
Sbjct: 153 LKILDMSGLKLSALSQIKLM----TAITTIDDLNYPEKTETYYVVNVPYIFSACWKTIKP 208
Query: 297 LLNEKNKKRLYV-HNQNYEEMYKYIPKDILP 208
LL E+ KK++ V +E+ K + + LP
Sbjct: 209 LLQERTKKKIQVLKGCGKDELLKIMDYESLP 239
>At1g75170.1 68414.m08731 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
polyphosphoinositide binding protein Ssh1p (GI:2739044)
{Glycine max}; similar to SEC14 cytosolic factor
(Phosphatidylinositol/phosphatidylcholine transfer
protein) (PI/PCTP) (SP:P24859) [Kluyveromyces lactis]
and to SEC14 cytosolic factor (SP:P53989) [Candida
glabrata]
Length = 296
Score = 31.1 bits (67), Expect = 0.68
Identities = 25/95 (26%), Positives = 45/95 (47%), Gaps = 4/95 (4%)
Frame = -3
Query: 384 QDALPVRLKGTHYLNTPTGFETVFNAVKALLNEKNKKRL-YVHNQNYEE---MYKYIPKD 217
Q+ P RL N P FE + VK ++ K ++ +V+ +N E M + ++
Sbjct: 173 QNHYPERLAVAFLYNPPRLFEAFWKIVKYFIDAKTFVKVKFVYPKNSESVELMSTFFDEE 232
Query: 216 ILPVEYGGNGGSIEEIIDYWKKKVKEYSDWLEEDD 112
LP E+GG + ++ Y +E+S + +DD
Sbjct: 233 NLPTEFGG-----KALLQY---NYEEFSKQMNQDD 259
>At1g09890.1 68414.m01113 expressed protein ; expression supported
by MPSS
Length = 633
Score = 30.7 bits (66), Expect = 0.90
Identities = 11/42 (26%), Positives = 21/42 (50%)
Frame = -3
Query: 234 KYIPKDILPVEYGGNGGSIEEIIDYWKKKVKEYSDWLEEDDQ 109
+Y+ KD + G G ++ W+++ KEY W D++
Sbjct: 346 RYVDKDFIAANRGYVGLAVPGAAGSWQRECKEYQFWTRTDEE 387
>At2g36690.1 68415.m04501 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to IDS3 [Hordeum
vulgare][GI:4514655], leucoanthocyanidin dioxygenase
[SP|P51091][Malus domestica]; contains PF03171
2OG-Fe(II) oxygenase superfamily domain
Length = 366
Score = 29.5 bits (63), Expect = 2.1
Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = -3
Query: 249 YEEMYKYIPKDI-LPVEYGGNGGSIEEIIDYWKKKVKEYS 133
YEE KY+ D+ PV YG + I++ + W+ +K Y+
Sbjct: 118 YEERSKYMSSDMSAPVRYGTSFNQIKDNVFCWRDFLKLYA 157
>At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
SEC14-like protein 2 (Alpha-tocopherol associated
protein) (TAP) (bTAP) (Fragment) (SP:P58875) {Bos
taurus}; similar to GI:807956 from [Saccharomyces
cerevisiae]; contains Pfam PF00650 : CRAL/TRIO domain;
contains Pfam PF03765 : CRAL/TRIO, N-terminus
Length = 573
Score = 29.5 bits (63), Expect = 2.1
Identities = 16/76 (21%), Positives = 38/76 (50%), Gaps = 3/76 (3%)
Frame = -3
Query: 411 FLKKMVVASQDALPVRLKGTHYLNTPTGFETVFNAVKALLNE---KNKKRLYVHNQNYEE 241
F+++ V +D P ++N P + + +++ ++K L +++ +
Sbjct: 387 FIRRAVKQFEDNYPEFAAKELFINVPWWYIPYYKTFGSIITSPRTRSKMVLAGPSKSADT 446
Query: 240 MYKYIPKDILPVEYGG 193
++KYI + +PV+YGG
Sbjct: 447 IFKYIAPEQVPVKYGG 462
>At5g64070.1 68418.m08046 phosphatidylinositol 4-kinase (PI4K)
nearly identical to gi:4467359
Length = 1121
Score = 28.7 bits (61), Expect = 3.6
Identities = 16/71 (22%), Positives = 36/71 (50%), Gaps = 7/71 (9%)
Frame = -3
Query: 249 YEEMYKYIPKDILPVEYGGNG--GSIEE---IIDYWKKKVKEYSDWLEEDDQYGTDE--S 91
++ +++ P+D P N G +E ++++K ++ +E+ + +G+ +
Sbjct: 460 FKRLFRIHPEDAKPTSENENSSNGLVESSPGTENFFRKLFRDRDQSVEDSELFGSKKHKE 519
Query: 90 KRPGKPKTAED 58
KRPG PK +D
Sbjct: 520 KRPGSPKQRDD 530
>At4g37900.1 68417.m05360 glycine-rich protein
Length = 787
Score = 28.7 bits (61), Expect = 3.6
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +3
Query: 366 ELVMRPEKQPPFFSRTLESFVINDLFLQ 449
+LV+ ++Q PFF + + V ND+FLQ
Sbjct: 164 DLVLAVKRQSPFFYQVSRAHVDNDVFLQ 191
>At3g10340.1 68416.m01240 phenylalanine ammonia-lyase, putative
similar to phenylalanine ammonia-lyase GB:S48726
[Petroselinum crispum]
Length = 707
Score = 28.3 bits (60), Expect = 4.8
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -3
Query: 195 GNGGSIEEIIDYWKKKVKEYSDWLEEDDQYGTD 97
G GGS E+ + + VK S+W+ E GTD
Sbjct: 65 GGGGSTVELAEEARAGVKASSEWVMESMNRGTD 97
>At5g51670.1 68418.m06406 expressed protein contains Pfam domain
PF05003: protein of unknown function (DUF668)
Length = 474
Score = 27.9 bits (59), Expect = 6.4
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = -1
Query: 281 IRNASTSTTRIMKRCTNISLRTFYQ 207
+ +A+ S +R+ RCT SLR+F++
Sbjct: 99 LAHAANSVSRLSNRCTTASLRSFHR 123
>At5g22990.1 68418.m02687 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 324
Score = 27.9 bits (59), Expect = 6.4
Identities = 15/35 (42%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = -3
Query: 291 NEKNKKRLYVHNQN-YEEMYKYIPKDILPVEYGGN 190
N +NKKRL NQ +EE P++I+ E GG+
Sbjct: 283 NARNKKRLRKRNQMIHEESNNIQPEEIVAEESGGD 317
>At3g48500.1 68416.m05294 expressed protein
Length = 668
Score = 27.5 bits (58), Expect = 8.4
Identities = 13/53 (24%), Positives = 30/53 (56%), Gaps = 7/53 (13%)
Frame = -3
Query: 195 GNGGSIEEI-IDYWKKKVKEYSDWLE------EDDQYGTDESKRPGKPKTAED 58
G +++++ ++ +K +K+Y +W+E E++ Y D+ PG+ K +D
Sbjct: 206 GEDATVDKMCLERFKVFLKQYKEWVEDNKDRLEEESYKLDQDFYPGRRKRGKD 258
>At2g41770.1 68415.m05163 expressed protein contains Pfam domain
PF03385: Protein of unknown function, DUF288
Length = 771
Score = 27.5 bits (58), Expect = 8.4
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = -3
Query: 318 VFNAVKALLNEKNKKRLYVHNQNYEEMYKYIPK 220
+F V L ++KN LYV + +YK++PK
Sbjct: 544 IFKTVVILSSQKNSD-LYVEEAKLDHIYKHLPK 575
>At2g19880.1 68415.m02324 ceramide glucosyltransferase, putative
similar to ceramide glucosyltransferase (GI:14718995)
[Gossypium arboreum]; weak similarity to Ceramide
glucosyltransferase (Glucosylceramide synthase) (GCS)
(UDP-glucose:N-acylsphingosine D-glucosyltransferase)
(GLCT-1) (Swiss-Prot:Q16739) [Homo sapiens]
Length = 519
Score = 27.5 bits (58), Expect = 8.4
Identities = 13/45 (28%), Positives = 26/45 (57%), Gaps = 2/45 (4%)
Frame = -1
Query: 647 WGQYWYYLRLQLLIL--HVSV*SYLADMMLISTIFPMSWLYL*SY 519
+G+YW YLR Q +L ++S +++ + L + +SW ++ Y
Sbjct: 307 FGRYWNYLRKQTFVLESYISKVNWIMNKALFAVHCYLSWGFVAPY 351
>At1g76460.1 68414.m08893 RNA recognition motif (RRM)-containing
protein low similarity to RRM-containing protein SEB-4
[Xenopus laevis] GI:8895698; contains Pfam profile:
PF00076 RNA recognition motif. (a.k.a. RRM, RBD, or RNP
domain)
Length = 285
Score = 27.5 bits (58), Expect = 8.4
Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%)
Frame = -3
Query: 372 PVRLKGTHYLNTPTGFETVFNA--VKALLNEKNKKRLYVHNQNYEEMYKYI 226
PV G HYLN+P G +T F V L E + L H + Y E+ + +
Sbjct: 5 PVPGSGFHYLNSPFG-DTTFTKVFVGGLAWETQSETLRQHFEQYGEILEAV 54
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,953,619
Number of Sequences: 28952
Number of extensions: 333156
Number of successful extensions: 1067
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1034
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1065
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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