Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV11a01f
(596 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g25540.1 68414.m03171 phytochrome and flowering time regulato... 33 0.11
At3g18840.1 68416.m02392 pentatricopeptide (PPR) repeat-containi... 30 1.0
At5g62580.1 68418.m07855 expressed protein 29 2.4
At1g20060.1 68414.m02511 kinesin motor protein-related 29 3.1
At4g25270.1 68417.m03635 pentatricopeptide (PPR) repeat-containi... 28 4.1
At3g25500.1 68416.m03171 formin homology 2 domain-containing pro... 28 4.1
At3g20540.1 68416.m02600 DNA-directed DNA polymerase family prot... 28 5.4
At1g56520.1 68414.m06499 disease resistance protein (TIR-NBS-LRR... 28 5.4
At1g17440.2 68414.m02133 transcription initiation factor IID (TF... 28 5.4
At1g17440.1 68414.m02132 transcription initiation factor IID (TF... 28 5.4
At5g63290.1 68418.m07943 coproporphyrinogen oxidase-related low ... 27 7.2
At2g22610.1 68415.m02680 kinesin motor protein-related 27 7.2
At3g53380.1 68416.m05891 lectin protein kinase family protein co... 27 9.5
At3g28520.1 68416.m03562 AAA-type ATPase family protein contains... 27 9.5
At1g62480.1 68414.m07050 vacuolar calcium-binding protein-relate... 27 9.5
>At1g25540.1 68414.m03171 phytochrome and flowering time regulatory
protein (PFT1) PMID: 12815435
Length = 836
Score = 33.5 bits (73), Expect = 0.11
Identities = 29/133 (21%), Positives = 57/133 (42%), Gaps = 8/133 (6%)
Frame = -1
Query: 551 VFQLVHPRCSQGRLVNRRPQLEHRRCLRS*QNQPGH----RRPQLEHRRCLRSLQNQPGH 384
VF+ P Q + Q + ++ ++ Q Q H + PQL+ ++ Q Q H
Sbjct: 673 VFKPQIPNQQQQQQQQLHQQQQQQQQIQQQQQQQQHLQQQQMPQLQQQQQQHQQQQQQQH 732
Query: 383 RRPQLEHRGFLRSLQSQPGNRHSQLELRHFLRSLQNQQGNRRCQREF----QCQKSQRCL 216
+ QL+H + Q Q +H +L+H + Q + Q++ Q Q+ L
Sbjct: 733 QLSQLQHHQQQQQQQQQQQQQHQLTQLQHHHQQQQQASPLNQMQQQTSPLNQMQQQTSPL 792
Query: 215 HFRSIQSRPRRVL 177
+ Q +P++++
Sbjct: 793 NQMQQQQQPQQMV 805
>At3g18840.1 68416.m02392 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 698
Score = 30.3 bits (65), Expect = 1.0
Identities = 13/44 (29%), Positives = 25/44 (56%)
Frame = -2
Query: 349 EAYRVSREIGILNWSFDIFYEVYRINREIDVVNGNFNVKNRNVV 218
EAY +S E G D++ + YR+++ I+++ G V+ V+
Sbjct: 522 EAYNISPETGHYTCMIDLYGKAYRLDKAIELMEGIDQVEKDAVI 565
>At5g62580.1 68418.m07855 expressed protein
Length = 615
Score = 29.1 bits (62), Expect = 2.4
Identities = 16/35 (45%), Positives = 19/35 (54%)
Frame = +3
Query: 84 HQSNPSKSRISQYRNTKASTLECTSSPTKSFKNSP 188
H S S SR+S YRN A T E +S + F SP
Sbjct: 541 HDSKYSASRMSTYRNRNAETTEIQNSRHR-FNGSP 574
>At1g20060.1 68414.m02511 kinesin motor protein-related
Length = 951
Score = 28.7 bits (61), Expect = 3.1
Identities = 29/105 (27%), Positives = 42/105 (40%), Gaps = 8/105 (7%)
Frame = +2
Query: 116 PIPKYESIDAGM--YIIPD-EVIQELALAGIELSENVDNVAIFDIEIPVDNVDFPVDSVN 286
P+P ++D I P E E + LS+NV + + + +N P SVN
Sbjct: 692 PVPNVRNVDVDSCNLINPRREASTEESSESPVLSKNVKDAELVPCHLSSENDAEPRQSVN 751
Query: 287 FVENVEAPVENADFPADSVSFV--ENLDAPV---EDADVLADSVN 406
ENV P A+ F +N D P E +V D +N
Sbjct: 752 SEENVGIPSTITHVEAEVTDFQRDQNQDDPTPSPEQVEVSQDCIN 796
>At4g25270.1 68417.m03635 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 527
Score = 28.3 bits (60), Expect = 4.1
Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +3
Query: 51 LSSHSLERRPPHQSNPSKSRISQYRNTKASTLECTS-SPT 167
+SS S++++P H + R +QY N ++L T SPT
Sbjct: 16 VSSSSMKKKPRHHQQLKQHRQNQYNNNGFTSLSFTKPSPT 55
>At3g25500.1 68416.m03171 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 1051
Score = 28.3 bits (60), Expect = 4.1
Identities = 16/51 (31%), Positives = 26/51 (50%)
Frame = +3
Query: 51 LSSHSLERRPPHQSNPSKSRISQYRNTKASTLECTSSPTKSFKNSPWPGLN 203
+S E +PP S P + ++ YR ++ +L +S + KNS GLN
Sbjct: 306 MSPKRSEPKPPVISTPEPAELTDYRFVRSPSLS-LASLSSGLKNSDEVGLN 355
>At3g20540.1 68416.m02600 DNA-directed DNA polymerase family protein
similar to PolI-like DNA polymerase [Oryza sativa]
GI:19912795; contains Pfam profiles PF01612: 3'-5'
exonuclease, PF00476: DNA polymerase I family A
Length = 1034
Score = 27.9 bits (59), Expect = 5.4
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = +3
Query: 102 KSRISQYRNTKASTLECTSSPTKSFKNSPWPGLN 203
K R +++RN K + PT+ F S WP ++
Sbjct: 590 KKRATKFRNIKLHRISDRPLPTEKFTASGWPSVS 623
>At1g56520.1 68414.m06499 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 897
Score = 27.9 bits (59), Expect = 5.4
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = -2
Query: 457 ISRDIGVLNWSIDVVYEVYRISQDIGVLNWSIEVFYE 347
+S++ +WS+D + E+ + +DIG + + VFYE
Sbjct: 73 LSKNYASSSWSLDELLEILKCKEDIGQI--VMTVFYE 107
>At1g17440.2 68414.m02133 transcription initiation factor IID
(TFIID) subunit A family protein similar to SP|Q16514
Transcription initiation factor TFIID 20/15 kDa subunits
(TAFII-20/TAFII-15) {Homo sapiens}; contains Pfam
profile PF03847: Transcription initiation factor TFIID
subunit A
Length = 683
Score = 27.9 bits (59), Expect = 5.4
Identities = 24/79 (30%), Positives = 35/79 (44%)
Frame = -1
Query: 461 QNQPGHRRPQLEHRRCLRSLQNQPGHRRPQLEHRGFLRSLQSQPGNRHSQLELRHFLRSL 282
Q Q PQL H+ + Q Q Q + + L QSQ HSQ +L+ + L
Sbjct: 402 QQQQQLSSPQL-HQSSMSLNQQQISQIIQQQQQQSQLG--QSQMNQSHSQQQLQQMQQQL 458
Query: 281 QNQQGNRRCQREFQCQKSQ 225
Q Q + Q++ Q Q+ Q
Sbjct: 459 QQQPQQQMQQQQQQQQQMQ 477
>At1g17440.1 68414.m02132 transcription initiation factor IID
(TFIID) subunit A family protein similar to SP|Q16514
Transcription initiation factor TFIID 20/15 kDa subunits
(TAFII-20/TAFII-15) {Homo sapiens}; contains Pfam
profile PF03847: Transcription initiation factor TFIID
subunit A
Length = 683
Score = 27.9 bits (59), Expect = 5.4
Identities = 24/79 (30%), Positives = 35/79 (44%)
Frame = -1
Query: 461 QNQPGHRRPQLEHRRCLRSLQNQPGHRRPQLEHRGFLRSLQSQPGNRHSQLELRHFLRSL 282
Q Q PQL H+ + Q Q Q + + L QSQ HSQ +L+ + L
Sbjct: 402 QQQQQLSSPQL-HQSSMSLNQQQISQIIQQQQQQSQLG--QSQMNQSHSQQQLQQMQQQL 458
Query: 281 QNQQGNRRCQREFQCQKSQ 225
Q Q + Q++ Q Q+ Q
Sbjct: 459 QQQPQQQMQQQQQQQQQMQ 477
>At5g63290.1 68418.m07943 coproporphyrinogen oxidase-related low
similarity to coproporphyrinogen III oxidase from
Geobacillus stearothermophilus [GI:2104798]; contains
Pfam profile PF04055: radical SAM domain protein
Length = 484
Score = 27.5 bits (58), Expect = 7.2
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -2
Query: 484 IDVVYEADRISRDIGVLNWSIDVV 413
+ VYEA ++ GV NWS+D++
Sbjct: 210 VSQVYEAIEFVKECGVENWSMDLI 233
>At2g22610.1 68415.m02680 kinesin motor protein-related
Length = 1093
Score = 27.5 bits (58), Expect = 7.2
Identities = 18/57 (31%), Positives = 34/57 (59%), Gaps = 2/57 (3%)
Frame = +2
Query: 242 EIPVDNVDFPVDSVNFVENVEAP-VENADFPADSVSFVENLDAPVEDA-DVLADSVN 406
++ +D+ FPVD +N V ++ +P + + DF +D +E D ++D D L DS++
Sbjct: 3 DVQIDDT-FPVD-LNGVTSLCSPEIPSFDFVSDETEKLEIGDTSIDDCDDALGDSMD 57
>At3g53380.1 68416.m05891 lectin protein kinase family protein
contains Pfam domains, PF00069: Protein kinase domain,
PF00138: Legume lectins alpha domain, and PF00139:
Legume lectins beta domain
Length = 715
Score = 27.1 bits (57), Expect = 9.5
Identities = 14/44 (31%), Positives = 23/44 (52%)
Frame = +3
Query: 57 SHSLERRPPHQSNPSKSRISQYRNTKASTLECTSSPTKSFKNSP 188
++S++ PP S PS S I NT+ T +S ++ K +P
Sbjct: 272 ANSVKSPPPLASQPSSSAIPISSNTQLKTSSSSSCHSRFCKENP 315
>At3g28520.1 68416.m03562 AAA-type ATPase family protein contains
Pfam profile: ATPase family PF00004
Length = 478
Score = 27.1 bits (57), Expect = 9.5
Identities = 15/45 (33%), Positives = 28/45 (62%), Gaps = 3/45 (6%)
Frame = -2
Query: 382 GVLNWSIEVFY---EAYRVSREIGILNWSFDIFYEVYRINREIDV 257
G +++ IE+ Y EA++V + + N S D++ E+ R+ E+DV
Sbjct: 368 GRMDYHIEMSYCRFEAFKVLAKNYLENESHDLYGEIGRLLEEVDV 412
>At1g62480.1 68414.m07050 vacuolar calcium-binding protein-related
contains weak similarity to vacuolar calcium binding
protein [Raphanus sativus] gi|9049359|dbj|BAA99394
Length = 152
Score = 27.1 bits (57), Expect = 9.5
Identities = 19/69 (27%), Positives = 33/69 (47%), Gaps = 3/69 (4%)
Frame = +2
Query: 188 LAGIELSENVDNVAIFDIEIPVDNVDFPVDSVNFVENVEAPVENADFPADSVS---FVEN 358
+A IE+ E V VA +IE+P + E++ APV + P + + VE
Sbjct: 1 MATIEV-EQVTPVAAENIEVPPPKAVESEEVTTVSESLPAPVTESQAPVEVTTKDLVVEE 59
Query: 359 LDAPVEDAD 385
+ P+E+ +
Sbjct: 60 TEKPIEETE 68
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,789,740
Number of Sequences: 28952
Number of extensions: 246280
Number of successful extensions: 862
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 803
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 860
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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