BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10p23f (648 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22A12.10 |||diacylglycerol cholinephosphotranferase/ diacylg... 101 7e-23 SPCC622.11 |||LMBR1-like membrane protein|Schizosaccharomyces po... 29 0.44 SPCC613.01 ||SPCC757.14|membrane transporter|Schizosaccharomyces... 27 1.8 SPBC21D10.11c |nfs1||iron-sulfur cluster assembly protein Nfs1|S... 26 5.4 SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 25 9.4 SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 25 9.4 >SPAC22A12.10 |||diacylglycerol cholinephosphotranferase/ diacylglycerol ethanolaminesphotranferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 386 Score = 101 bits (243), Expect = 7e-23 Identities = 54/130 (41%), Positives = 76/130 (58%), Gaps = 1/130 (0%) Frame = +3 Query: 255 LSQNHFDGLKKYKYSAIDTSPLSNYVMHPSW-NFITRFIPKWIAPNLITFAGFVCMVIVI 431 L++ L YKYSAID S LS Y++ P W N + + IP +APNLIT G +VI I Sbjct: 3 LNRKQLKNLHNYKYSAIDNSLLSKYILKPYWWNQLLKVIPMSMAPNLITLIGLGFVVINI 62 Query: 432 LLLTIFDYDFHGAERLRQGVDEYRIPNWALMACGVLLFLAYNLDGIDGKQARKLGVSGPL 611 L + ++ Y + +D + P+W + + LFL + D IDG QAR+ G S PL Sbjct: 63 LTMLVYKYHYE--------MDAF--PSWVYASWAIGLFLYQSFDAIDGSQARRTGTSSPL 112 Query: 612 GELFDHGLDS 641 G+LFDHG+D+ Sbjct: 113 GQLFDHGVDA 122 >SPCC622.11 |||LMBR1-like membrane protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 562 Score = 29.5 bits (63), Expect = 0.44 Identities = 19/63 (30%), Positives = 31/63 (49%) Frame = -3 Query: 232 SMVVIRFLQILTRLQGQKMLVIFRNKNKLLFSFVTFDLNTSLYANLTFFRI*TLLFCIVM 53 S+ + RFL LT + +L + + FS L+ S Y NL ++ + T L C+V+ Sbjct: 116 SLSIWRFLYWLTFVLSWIILPFVQGYMESKFSTPRSRLSDSFYKNLRYYLLLTFLTCVVI 175 Query: 52 IIL 44 L Sbjct: 176 AYL 178 >SPCC613.01 ||SPCC757.14|membrane transporter|Schizosaccharomyces pombe|chr 3|||Manual Length = 497 Score = 27.5 bits (58), Expect = 1.8 Identities = 12/45 (26%), Positives = 25/45 (55%) Frame = +3 Query: 318 LSNYVMHPSWNFITRFIPKWIAPNLITFAGFVCMVIVILLLTIFD 452 LS+ V+ P + F + F+ KW+ I GF+ ++ +++ + D Sbjct: 353 LSSLVIAP-FVFFSTFLSKWLEDRQIMLYGFMMGIVALIVHLVLD 396 >SPBC21D10.11c |nfs1||iron-sulfur cluster assembly protein Nfs1|Schizosaccharomyces pombe|chr 2|||Manual Length = 498 Score = 25.8 bits (54), Expect = 5.4 Identities = 16/52 (30%), Positives = 28/52 (53%), Gaps = 5/52 (9%) Frame = +3 Query: 216 RITTMEKLFEYKY---LSQNHFDGLKKYKYSAIDTSPLSNY--VMHPSWNFI 356 RI E ++Y + LSQ DGL Y++++ P S Y ++ S+N++ Sbjct: 351 RICKEEMKYDYAHISKLSQRLIDGLLAIPYTSLNGDPKSRYPGCVNISFNYV 402 >SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 331 Score = 25.0 bits (52), Expect = 9.4 Identities = 12/29 (41%), Positives = 19/29 (65%), Gaps = 1/29 (3%) Frame = +3 Query: 309 TSPLSNYVMHPSWNFIT-RFIPKWIAPNL 392 TSPL +Y +N ++ +F+PKW +P L Sbjct: 225 TSPL-DYTAKVQFNRLSGKFMPKWASPEL 252 >SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2100 Score = 25.0 bits (52), Expect = 9.4 Identities = 9/17 (52%), Positives = 13/17 (76%) Frame = +3 Query: 18 LYESACSVNKIIITIQN 68 LYE S+ KI++T+QN Sbjct: 677 LYERRISIGKIVLTLQN 693 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,721,015 Number of Sequences: 5004 Number of extensions: 58660 Number of successful extensions: 144 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 138 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 142 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 291768710 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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