BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10o16r (650 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po... 29 0.44 SPAC1002.15c |pmc5|med6|mediator complex subunit Pmc5 |Schizosac... 27 1.8 SPCC24B10.02c |||NAD/NADH kinase|Schizosaccharomyces pombe|chr 3... 27 2.3 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 2.3 SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces po... 26 4.1 SPBC13A2.02 |||nucleoporin Nup82|Schizosaccharomyces pombe|chr 2... 26 5.4 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 25 7.2 SPBC106.04 |ada1||adenosine deaminase Ada1 |Schizosaccharomyces ... 25 7.2 SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 7.2 SPBC1685.06 |cid11||poly|Schizosaccharomyces pombe|chr 2|||Manual 25 7.2 SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch... 25 7.2 SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 25 9.5 SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizo... 25 9.5 SPBC725.14 |arg6||acetylglutamate synthase Arg6 |Schizosaccharom... 25 9.5 SPAC23H4.15 |||ribosome biogenesis protein Tsr1 |Schizosaccharom... 25 9.5 >SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces pombe|chr 2|||Manual Length = 811 Score = 29.5 bits (63), Expect = 0.44 Identities = 26/80 (32%), Positives = 37/80 (46%), Gaps = 1/80 (1%) Frame = -3 Query: 267 ALENVVAEATASVSLADNIIVSDIGAAITIGDVKSNLQINLFGREVGPAVNNFLEKIPVY 88 A E +A S L N+ + AAIT K + + F +E G V E+I Sbjct: 74 AHEAFIASKIVSGDLKTNL---QVNAAITYCKDKDTIDESEFDKETGVGVVLTPEQIEQL 130 Query: 87 LTDYAAE-VSRVLEYVAQLV 31 + DY AE S++LE QL+ Sbjct: 131 VGDYVAENKSKILEQRYQLL 150 >SPAC1002.15c |pmc5|med6|mediator complex subunit Pmc5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 216 Score = 27.5 bits (58), Expect = 1.8 Identities = 18/43 (41%), Positives = 22/43 (51%), Gaps = 5/43 (11%) Frame = +1 Query: 223 EGDGSGSLSNDVLKGKSSGSERPQD-----AAENADFSFSGTD 336 EG LSND L+ S + P D + ENAD+SFS D Sbjct: 153 EGYTYPKLSNDNLEVDHSNTNEPADENKNQSIENADYSFSPED 195 >SPCC24B10.02c |||NAD/NADH kinase|Schizosaccharomyces pombe|chr 3|||Manual Length = 449 Score = 27.1 bits (57), Expect = 2.3 Identities = 14/40 (35%), Positives = 25/40 (62%), Gaps = 1/40 (2%) Frame = -3 Query: 189 AITIGDVKSNLQINLFGREVGPAVNNFL-EKIPVYLTDYA 73 AITIGD + L + +++GP V +F + +P +LT ++ Sbjct: 178 AITIGDNSTLLYTSWLFQKIGPPVLSFSDDDVPGFLTHFS 217 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 27.1 bits (57), Expect = 2.3 Identities = 13/41 (31%), Positives = 19/41 (46%) Frame = -2 Query: 160 PPNQSLRTRSWTSRQQLPREDPCLPDGLRSRGQPCSGVRRP 38 PP S+ S S +P + P +P L QP + V+ P Sbjct: 144 PPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSP 184 >SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 26.2 bits (55), Expect = 4.1 Identities = 11/40 (27%), Positives = 21/40 (52%) Frame = +1 Query: 196 DVTDNDVVCEGDGSGSLSNDVLKGKSSGSERPQDAAENAD 315 + DN ++ SGS + D + + G+E+ +D EN + Sbjct: 325 NAVDNKILLIAMSSGSEALDAILAQMGGTEKVEDVLENVN 364 >SPBC13A2.02 |||nucleoporin Nup82|Schizosaccharomyces pombe|chr 2|||Manual Length = 803 Score = 25.8 bits (54), Expect = 5.4 Identities = 21/87 (24%), Positives = 40/87 (45%), Gaps = 2/87 (2%) Frame = +1 Query: 28 DDELGDVLQNTADLGCVVRQVDRDLL*EVVDGWSNFASEEIDL--EVRFDIANSYCSSDV 201 +DEL +VL + V+ ++D + L E D A ++ + ++ ++ Sbjct: 479 EDELSNVLVSIPSRTSVLERLDTNPLNESTDAVVGCAQLYYPSLGKILISLTRNWQTTVF 538 Query: 202 TDNDVVCEGDGSGSLSNDVLKGKSSGS 282 D+D+ G SLSN++ KS G+ Sbjct: 539 DDSDLATMGVNKESLSNEMDYSKSLGT 565 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 25.4 bits (53), Expect = 7.2 Identities = 30/96 (31%), Positives = 48/96 (50%), Gaps = 4/96 (4%) Frame = -3 Query: 309 IFSRVLRSFASGGLALENVVAEATASVSLADNIIVSDIGAAITIGDVKSNLQINLFGREV 130 ++S VL+ A L+ V+A A S D VS + A+ D+ S L I L + V Sbjct: 952 LWSEVLQDSAYRRPLLDQVIATAVPESS--DPEAVSIVVKALMEVDLPSQL-IELLEKIV 1008 Query: 129 ----GPAVNNFLEKIPVYLTDYAAEVSRVLEYVAQL 34 + N L+ + ++LT A+ SRV+EY+ +L Sbjct: 1009 LQPSSFSENANLQNL-LFLTAIKADKSRVMEYIDKL 1043 >SPBC106.04 |ada1||adenosine deaminase Ada1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 846 Score = 25.4 bits (53), Expect = 7.2 Identities = 15/36 (41%), Positives = 17/36 (47%), Gaps = 1/36 (2%) Frame = +1 Query: 226 GDGSGSLSNDVLKGKSSGSERPQ-DAAENADFSFSG 330 G GSG L ND L+ K S P +NA S G Sbjct: 117 GPGSGKLLNDTLQSKISSIHMPHVQQGDNAVVSSVG 152 >SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1038 Score = 25.4 bits (53), Expect = 7.2 Identities = 22/56 (39%), Positives = 26/56 (46%) Frame = +1 Query: 214 VVCEGDGSGSLSNDVLKGKSSGSERPQDAAENADFSFSGTD*C*NSGNSQD*VESG 381 VV E D +LS LK ++G +AEN S T NSG S D V SG Sbjct: 817 VVQEEDQRDTLS---LKTSTTGLSSHSKSAENNSTQQSTTSPSINSGASADAVSSG 869 >SPBC1685.06 |cid11||poly|Schizosaccharomyces pombe|chr 2|||Manual Length = 478 Score = 25.4 bits (53), Expect = 7.2 Identities = 10/24 (41%), Positives = 15/24 (62%) Frame = +1 Query: 514 ERISRSQQFTDFIEQV*DHDVKDA 585 E +SR QQF D + + ++KDA Sbjct: 63 EEVSRRQQFVDKLRTILSTEIKDA 86 >SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1935 Score = 25.4 bits (53), Expect = 7.2 Identities = 12/41 (29%), Positives = 24/41 (58%) Frame = -3 Query: 147 LFGREVGPAVNNFLEKIPVYLTDYAAEVSRVLEYVAQLVIN 25 + R++GP V F+ K+P L + ++ + + V +LV+N Sbjct: 972 IHNRKMGPTVKKFISKLP--LLNINVDLLPLTKNVLRLVLN 1010 >SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 1106 Score = 25.0 bits (52), Expect = 9.5 Identities = 9/23 (39%), Positives = 14/23 (60%) Frame = -3 Query: 102 KIPVYLTDYAAEVSRVLEYVAQL 34 K+P + DY EV R+++ A L Sbjct: 968 KVPTFYADYVNEVKRIIQRNANL 990 >SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizosaccharomyces pombe|chr 3|||Manual Length = 388 Score = 25.0 bits (52), Expect = 9.5 Identities = 21/58 (36%), Positives = 29/58 (50%), Gaps = 3/58 (5%) Frame = -3 Query: 330 SAEGEIRIFSRVLRSFASGGLALENVVAEA--TASVSLADNIIVSDI-GAAITIGDVK 166 S +GEI + V + + G L NVV EA A D +I SD+ AA + D+K Sbjct: 320 SKQGEIDYEALVKLTDGTNGADLRNVVTEAGFIAIKEDRDYVIQSDLMSAARKVADLK 377 >SPBC725.14 |arg6||acetylglutamate synthase Arg6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 500 Score = 25.0 bits (52), Expect = 9.5 Identities = 9/14 (64%), Positives = 11/14 (78%) Frame = +2 Query: 140 PKRLIWRLDLTSPI 181 PK LIWR LT+P+ Sbjct: 436 PKELIWRSRLTNPV 449 >SPAC23H4.15 |||ribosome biogenesis protein Tsr1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 783 Score = 25.0 bits (52), Expect = 9.5 Identities = 15/43 (34%), Positives = 22/43 (51%), Gaps = 1/43 (2%) Frame = +1 Query: 193 SDVTDNDVVCEGDGSGSLSNDVLKGK-SSGSERPQDAAENADF 318 SDV D +V+ E + +SN V + K S E D A++ F Sbjct: 404 SDVEDTEVIIEDQDNQEISNHVAEEKIDSDEEETIDDAKSEMF 446 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,326,809 Number of Sequences: 5004 Number of extensions: 42840 Number of successful extensions: 151 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 150 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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