BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10o04r (651 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF... 100 4e-22 SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F ... 81 2e-16 SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Sc... 26 5.4 SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase... 26 5.4 SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual 26 5.4 SPBC2D10.09 |||3-hydroxyisobutyryl-CoA hydrolase|Schizosaccharom... 25 7.2 SPBC651.03c |gyp10||GTPase activating protein Gyp10|Schizosaccha... 25 9.5 SPBC1709.14 |||peptide N-glycanase |Schizosaccharomyces pombe|ch... 25 9.5 >SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF4E 4F complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 243 Score = 99.5 bits (237), Expect = 4e-22 Identities = 60/182 (32%), Positives = 88/182 (48%), Gaps = 7/182 (3%) Frame = -1 Query: 537 DGDTSLQSESSPAIKHPLEHTWSYWLYTNKSK--EWIHNLVELSTFATVEDYWCLYHYMK 364 +G +++ +E++ HPL+H W+ W ++ EW L E+ +F TVE++W ++ + Sbjct: 49 EGLSAVNAETAFVKTHPLQHEWTLWFLKPPTQGLEWSDLLKEIISFKTVEEFWGIFKTIS 108 Query: 363 LPSELNHGQDYMIFKKGIQPTWEDPINEKGGRWIICESKSARNXXXXXXXXXXLMIGENF 184 S L DY F KGI+P WEDP N GG+W N IGE Sbjct: 109 KASMLPAKSDYSYFLKGIRPEWEDPQNMNGGKWAYQSKHKGSNLDELWLYMVLAAIGETL 168 Query: 183 EHPDL-ICGVVVNI-KAKSKISLWTRG-EDKQKNMKIGKTVKEKLNIN--RNISYFNHNT 19 + + GVV N+ K +I++WTR DK KIG KE L I+ I Y H Sbjct: 169 DPTGKEVTGVVCNMRKGFYRIAVWTRNCNDKDVLEKIGLRFKEVLGISDKETIEYSAHED 228 Query: 18 TS 13 +S Sbjct: 229 SS 230 >SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 218 Score = 80.6 bits (190), Expect = 2e-16 Identities = 47/170 (27%), Positives = 84/170 (49%), Gaps = 8/170 (4%) Frame = -1 Query: 498 IKHPLEHTWSYWLYTNKSK--EWIHNLVELSTFATVEDYWCLYHYMKLPSELNHGQDYMI 325 +KHPL W+ W + EW + TF +VE++W +++ + S L DY Sbjct: 36 LKHPLARPWTLWFLMPPTPGLEWNELQKNIITFNSVEEFWGIHNNINPASSLPIKSDYSF 95 Query: 324 FKKGIQPTWEDPINEKGGRWII-CESKSARNXXXXXXXXXXLMIGENFEHP-DLICGVVV 151 F++G++P WED N+ GG+W + + IGE + + GVV+ Sbjct: 96 FREGVRPEWEDVHNKTGGKWAFQNKGRGGNALDEMWLTTVLAAIGETLDPTGQEVMGVVI 155 Query: 150 NI-KAKSKISLWTRG-EDKQKNMKIGKTVKEKLNINRN--ISYFNHNTTS 13 N+ K ++++WT+ +++ M+IG K+ LN+ R+ I + H +S Sbjct: 156 NMRKGFYRLAVWTKSCNNREVLMEIGTRFKQVLNLPRSETIEFSAHEDSS 205 >SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1233 Score = 25.8 bits (54), Expect = 5.4 Identities = 9/22 (40%), Positives = 15/22 (68%) Frame = +2 Query: 191 SPIISRIRSNHRGSRLRADLLS 256 SP+ + R H+G+RL D+L+ Sbjct: 586 SPVNQKFRGTHKGARLAIDVLN 607 >SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase|Schizosaccharomyces pombe|chr 2|||Manual Length = 362 Score = 25.8 bits (54), Expect = 5.4 Identities = 9/28 (32%), Positives = 15/28 (53%) Frame = -1 Query: 165 CGVVVNIKAKSKISLWTRGEDKQKNMKI 82 CG++ S + +W R + KQK+ I Sbjct: 325 CGIIYQFITDSNVGMWCRVKRKQKHADI 352 >SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual Length = 935 Score = 25.8 bits (54), Expect = 5.4 Identities = 11/22 (50%), Positives = 13/22 (59%) Frame = +3 Query: 528 YHHLASHVDWTVQTSLLIFFKI 593 + H V W+V TSLL FF I Sbjct: 597 FSHKTISVSWSVLTSLLCFFTI 618 >SPBC2D10.09 |||3-hydroxyisobutyryl-CoA hydrolase|Schizosaccharomyces pombe|chr 2|||Manual Length = 429 Score = 25.4 bits (53), Expect = 7.2 Identities = 11/28 (39%), Positives = 15/28 (53%) Frame = -1 Query: 381 LYHYMKLPSELNHGQDYMIFKKGIQPTW 298 L +Y KLPSE N+G + + P W Sbjct: 394 LENYFKLPSEYNNGISFAAKGRRKTPLW 421 >SPBC651.03c |gyp10||GTPase activating protein Gyp10|Schizosaccharomyces pombe|chr 2|||Manual Length = 373 Score = 25.0 bits (52), Expect = 9.5 Identities = 15/44 (34%), Positives = 23/44 (52%) Frame = +3 Query: 342 HGLTLKVVSCSGINTSNLLQ*RMLIIQPSCVSILYSYLCKASSS 473 H LT+ V +C+ + N L+ S +L+SYLCK +S Sbjct: 220 HPLTV-VYTCAQVVLDNRTSIIELLWDNSGADLLHSYLCKLPAS 262 >SPBC1709.14 |||peptide N-glycanase |Schizosaccharomyces pombe|chr 2|||Manual Length = 333 Score = 25.0 bits (52), Expect = 9.5 Identities = 7/18 (38%), Positives = 13/18 (72%) Frame = -1 Query: 483 EHTWSYWLYTNKSKEWIH 430 +H W+ +Y+NK + W+H Sbjct: 189 DHVWTE-VYSNKQQRWVH 205 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,651,280 Number of Sequences: 5004 Number of extensions: 54631 Number of successful extensions: 150 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 146 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 149 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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