SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV10o04r
         (651 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF...   100   4e-22
SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F ...    81   2e-16
SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Sc...    26   5.4  
SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase...    26   5.4  
SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual     26   5.4  
SPBC2D10.09 |||3-hydroxyisobutyryl-CoA hydrolase|Schizosaccharom...    25   7.2  
SPBC651.03c |gyp10||GTPase activating protein Gyp10|Schizosaccha...    25   9.5  
SPBC1709.14 |||peptide N-glycanase |Schizosaccharomyces pombe|ch...    25   9.5  

>SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF4E
           4F complex subunit |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 243

 Score = 99.5 bits (237), Expect = 4e-22
 Identities = 60/182 (32%), Positives = 88/182 (48%), Gaps = 7/182 (3%)
 Frame = -1

Query: 537 DGDTSLQSESSPAIKHPLEHTWSYWLYTNKSK--EWIHNLVELSTFATVEDYWCLYHYMK 364
           +G +++ +E++    HPL+H W+ W     ++  EW   L E+ +F TVE++W ++  + 
Sbjct: 49  EGLSAVNAETAFVKTHPLQHEWTLWFLKPPTQGLEWSDLLKEIISFKTVEEFWGIFKTIS 108

Query: 363 LPSELNHGQDYMIFKKGIQPTWEDPINEKGGRWIICESKSARNXXXXXXXXXXLMIGENF 184
             S L    DY  F KGI+P WEDP N  GG+W         N            IGE  
Sbjct: 109 KASMLPAKSDYSYFLKGIRPEWEDPQNMNGGKWAYQSKHKGSNLDELWLYMVLAAIGETL 168

Query: 183 EHPDL-ICGVVVNI-KAKSKISLWTRG-EDKQKNMKIGKTVKEKLNIN--RNISYFNHNT 19
           +     + GVV N+ K   +I++WTR   DK    KIG   KE L I+    I Y  H  
Sbjct: 169 DPTGKEVTGVVCNMRKGFYRIAVWTRNCNDKDVLEKIGLRFKEVLGISDKETIEYSAHED 228

Query: 18  TS 13
           +S
Sbjct: 229 SS 230


>SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F
           complex subunit |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 218

 Score = 80.6 bits (190), Expect = 2e-16
 Identities = 47/170 (27%), Positives = 84/170 (49%), Gaps = 8/170 (4%)
 Frame = -1

Query: 498 IKHPLEHTWSYWLYTNKSK--EWIHNLVELSTFATVEDYWCLYHYMKLPSELNHGQDYMI 325
           +KHPL   W+ W     +   EW      + TF +VE++W +++ +   S L    DY  
Sbjct: 36  LKHPLARPWTLWFLMPPTPGLEWNELQKNIITFNSVEEFWGIHNNINPASSLPIKSDYSF 95

Query: 324 FKKGIQPTWEDPINEKGGRWII-CESKSARNXXXXXXXXXXLMIGENFEHP-DLICGVVV 151
           F++G++P WED  N+ GG+W    + +                IGE  +     + GVV+
Sbjct: 96  FREGVRPEWEDVHNKTGGKWAFQNKGRGGNALDEMWLTTVLAAIGETLDPTGQEVMGVVI 155

Query: 150 NI-KAKSKISLWTRG-EDKQKNMKIGKTVKEKLNINRN--ISYFNHNTTS 13
           N+ K   ++++WT+   +++  M+IG   K+ LN+ R+  I +  H  +S
Sbjct: 156 NMRKGFYRLAVWTKSCNNREVLMEIGTRFKQVLNLPRSETIEFSAHEDSS 205


>SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1233

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 9/22 (40%), Positives = 15/22 (68%)
 Frame = +2

Query: 191 SPIISRIRSNHRGSRLRADLLS 256
           SP+  + R  H+G+RL  D+L+
Sbjct: 586 SPVNQKFRGTHKGARLAIDVLN 607


>SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4
           desaturase|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 362

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 9/28 (32%), Positives = 15/28 (53%)
 Frame = -1

Query: 165 CGVVVNIKAKSKISLWTRGEDKQKNMKI 82
           CG++      S + +W R + KQK+  I
Sbjct: 325 CGIIYQFITDSNVGMWCRVKRKQKHADI 352


>SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 935

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 11/22 (50%), Positives = 13/22 (59%)
 Frame = +3

Query: 528 YHHLASHVDWTVQTSLLIFFKI 593
           + H    V W+V TSLL FF I
Sbjct: 597 FSHKTISVSWSVLTSLLCFFTI 618


>SPBC2D10.09 |||3-hydroxyisobutyryl-CoA
           hydrolase|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 429

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 11/28 (39%), Positives = 15/28 (53%)
 Frame = -1

Query: 381 LYHYMKLPSELNHGQDYMIFKKGIQPTW 298
           L +Y KLPSE N+G  +    +   P W
Sbjct: 394 LENYFKLPSEYNNGISFAAKGRRKTPLW 421


>SPBC651.03c |gyp10||GTPase activating protein
           Gyp10|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 373

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 15/44 (34%), Positives = 23/44 (52%)
 Frame = +3

Query: 342 HGLTLKVVSCSGINTSNLLQ*RMLIIQPSCVSILYSYLCKASSS 473
           H LT+ V +C+ +   N      L+   S   +L+SYLCK  +S
Sbjct: 220 HPLTV-VYTCAQVVLDNRTSIIELLWDNSGADLLHSYLCKLPAS 262


>SPBC1709.14 |||peptide N-glycanase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 333

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 7/18 (38%), Positives = 13/18 (72%)
 Frame = -1

Query: 483 EHTWSYWLYTNKSKEWIH 430
           +H W+  +Y+NK + W+H
Sbjct: 189 DHVWTE-VYSNKQQRWVH 205


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,651,280
Number of Sequences: 5004
Number of extensions: 54631
Number of successful extensions: 150
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -