Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10o04f
(670 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF... 100 4e-22
SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F ... 81 2e-16
SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Sc... 26 5.6
SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase... 26 5.6
SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual 26 5.6
SPBC2D10.09 |||3-hydroxyisobutyryl-CoA hydrolase|Schizosaccharom... 25 7.5
SPBC651.03c |gyp10||GTPase activating protein Gyp10|Schizosaccha... 25 9.9
SPBC1709.14 |||peptide N-glycanase |Schizosaccharomyces pombe|ch... 25 9.9
>SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF4E
4F complex subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 243
Score = 99.5 bits (237), Expect = 4e-22
Identities = 60/182 (32%), Positives = 88/182 (48%), Gaps = 7/182 (3%)
Frame = +1
Query: 115 DGDTSLQSESSPAIKHPLEHTWSYWLYTNKSK--EWIHNLVELSTFATVEDYWCLYHYMK 288
+G +++ +E++ HPL+H W+ W ++ EW L E+ +F TVE++W ++ +
Sbjct: 49 EGLSAVNAETAFVKTHPLQHEWTLWFLKPPTQGLEWSDLLKEIISFKTVEEFWGIFKTIS 108
Query: 289 LPSELNHGQDYMIFKKGIQPTWEDPINEKGGRWIICESKSARNXXXXXXXXXXXMIGENF 468
S L DY F KGI+P WEDP N GG+W N IGE
Sbjct: 109 KASMLPAKSDYSYFLKGIRPEWEDPQNMNGGKWAYQSKHKGSNLDELWLYMVLAAIGETL 168
Query: 469 EHPDL-ICGVVVNI-KAKSKISLWTRG-EDKQKNMKIGKTVKEKLNIN--RNISYFNHNT 633
+ + GVV N+ K +I++WTR DK KIG KE L I+ I Y H
Sbjct: 169 DPTGKEVTGVVCNMRKGFYRIAVWTRNCNDKDVLEKIGLRFKEVLGISDKETIEYSAHED 228
Query: 634 TS 639
+S
Sbjct: 229 SS 230
>SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F
complex subunit |Schizosaccharomyces pombe|chr
1|||Manual
Length = 218
Score = 80.6 bits (190), Expect = 2e-16
Identities = 47/170 (27%), Positives = 84/170 (49%), Gaps = 8/170 (4%)
Frame = +1
Query: 154 IKHPLEHTWSYWLYTNKSK--EWIHNLVELSTFATVEDYWCLYHYMKLPSELNHGQDYMI 327
+KHPL W+ W + EW + TF +VE++W +++ + S L DY
Sbjct: 36 LKHPLARPWTLWFLMPPTPGLEWNELQKNIITFNSVEEFWGIHNNINPASSLPIKSDYSF 95
Query: 328 FKKGIQPTWEDPINEKGGRWII-CESKSARNXXXXXXXXXXXMIGENFEHP-DLICGVVV 501
F++G++P WED N+ GG+W + + IGE + + GVV+
Sbjct: 96 FREGVRPEWEDVHNKTGGKWAFQNKGRGGNALDEMWLTTVLAAIGETLDPTGQEVMGVVI 155
Query: 502 NI-KAKSKISLWTRG-EDKQKNMKIGKTVKEKLNINRN--ISYFNHNTTS 639
N+ K ++++WT+ +++ M+IG K+ LN+ R+ I + H +S
Sbjct: 156 NMRKGFYRLAVWTKSCNNREVLMEIGTRFKQVLNLPRSETIEFSAHEDSS 205
>SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 5.6
Identities = 9/22 (40%), Positives = 15/22 (68%)
Frame = -3
Query: 461 SPIISRIRSNHRGSRLRADLLS 396
SP+ + R H+G+RL D+L+
Sbjct: 586 SPVNQKFRGTHKGARLAIDVLN 607
>SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4
desaturase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 362
Score = 25.8 bits (54), Expect = 5.6
Identities = 9/28 (32%), Positives = 15/28 (53%)
Frame = +1
Query: 487 CGVVVNIKAKSKISLWTRGEDKQKNMKI 570
CG++ S + +W R + KQK+ I
Sbjct: 325 CGIIYQFITDSNVGMWCRVKRKQKHADI 352
>SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual
Length = 935
Score = 25.8 bits (54), Expect = 5.6
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = -1
Query: 124 YHHLASHVDWTVQTSLLIFFKI 59
+ H V W+V TSLL FF I
Sbjct: 597 FSHKTISVSWSVLTSLLCFFTI 618
>SPBC2D10.09 |||3-hydroxyisobutyryl-CoA
hydrolase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 429
Score = 25.4 bits (53), Expect = 7.5
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = +1
Query: 271 LYHYMKLPSELNHGQDYMIFKKGIQPTW 354
L +Y KLPSE N+G + + P W
Sbjct: 394 LENYFKLPSEYNNGISFAAKGRRKTPLW 421
>SPBC651.03c |gyp10||GTPase activating protein
Gyp10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 373
Score = 25.0 bits (52), Expect = 9.9
Identities = 15/44 (34%), Positives = 23/44 (52%)
Frame = -1
Query: 310 HGLTLKVVSCSGINTSNLLQ*RMLIIQPSCVSILYSYLCKASSS 179
H LT+ V +C+ + N L+ S +L+SYLCK +S
Sbjct: 220 HPLTV-VYTCAQVVLDNRTSIIELLWDNSGADLLHSYLCKLPAS 262
>SPBC1709.14 |||peptide N-glycanase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 333
Score = 25.0 bits (52), Expect = 9.9
Identities = 7/18 (38%), Positives = 13/18 (72%)
Frame = +1
Query: 169 EHTWSYWLYTNKSKEWIH 222
+H W+ +Y+NK + W+H
Sbjct: 189 DHVWTE-VYSNKQQRWVH 205
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,723,250
Number of Sequences: 5004
Number of extensions: 56160
Number of successful extensions: 151
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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