Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10o04f
(670 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g18040.1 68417.m02685 eukaryotic translation initiation facto... 138 4e-33
At5g35620.1 68418.m04251 eukaryotic translation initiation facto... 107 7e-24
At1g29550.1 68414.m03614 eukaryotic translation initiation facto... 107 9e-24
At1g29590.1 68414.m03618 eukaryotic translation initiation facto... 105 3e-23
At5g35620.2 68418.m04252 eukaryotic translation initiation facto... 94 9e-20
At5g18110.1 68418.m02126 novel cap-binding protein (nCBP) identi... 83 2e-16
At3g07770.1 68416.m00947 heat shock protein-related strong simil... 29 3.7
At2g35340.1 68415.m04333 RNA helicase, putative similar to ATP-d... 28 4.9
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 28 6.5
At1g64590.1 68414.m07321 short-chain dehydrogenase/reductase (SD... 28 6.5
At4g19510.2 68417.m02870 disease resistance protein (TIR-NBS-LRR... 27 8.5
At4g19510.1 68417.m02869 disease resistance protein (TIR-NBS-LRR... 27 8.5
>At4g18040.1 68417.m02685 eukaryotic translation initiation factor
4E 1 / eIF-4E1 / mRNA cap-binding protein 1 (EIF4E1)
identical to SP|O23252 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-4F 25 kDa subunit) (eIF-4F P26 subunit)
{Arabidopsis thaliana}
Length = 235
Score = 138 bits (333), Expect = 4e-33
Identities = 70/177 (39%), Positives = 100/177 (56%), Gaps = 6/177 (3%)
Frame = +1
Query: 115 DGDTSLQSESSPAIKHPLEHTWSYWL----YTNKSKEWIHNLVELSTFATVEDYWCLYHY 282
DG+ S+S HPLEH+W++W +K W +L + TF+TVE++W LY+
Sbjct: 45 DGNVDESSKSGVPESHPLEHSWTFWFDNPAVKSKQTSWGSSLRPVFTFSTVEEFWSLYNN 104
Query: 283 MKLPSELNHGQDYMIFKKGIQPTWEDPINEKGGRWIICESKSARNXXXXXXXXXXXMIGE 462
MK PS+L HG D+ FK I+P WEDPI GG+W + K + +IGE
Sbjct: 105 MKHPSKLAHGADFYCFKHIIEPKWEDPICANGGKWTMTFPKEKSD--KSWLYTLLALIGE 162
Query: 463 NFEHPDLICGVVVNIKAK-SKISLWTR-GEDKQKNMKIGKTVKEKLNINRNISYFNH 627
F+H D ICG VVNI+ K +IS+WT+ ++ + IGK KE L+ N +I + H
Sbjct: 163 QFDHGDEICGAVVNIRGKQERISIWTKNASNEAAQVSIGKQWKEFLDYNNSIGFIIH 219
>At5g35620.1 68418.m04251 eukaryotic translation initiation factor
4E 2 / eIF-4E2 / mRNA cap-binding protein 2 (EIF4E2)
identical to SP|O04663 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-(iso)4F 25 kDa subunit) (eIF-(ISO)4F P28 subunit)
(eIF4Eiso protein) {Arabidopsis thaliana}
Length = 198
Score = 107 bits (257), Expect = 7e-24
Identities = 60/172 (34%), Positives = 92/172 (53%), Gaps = 8/172 (4%)
Frame = +1
Query: 160 HPLEHTWSYWLYTNKSKE---WIHNLVELSTFATVEDYWCLYHYMKLPSELNHGQDYMIF 330
H LE WS+W + N+SK+ W +L + TF TVED+W L+ + S+L + +F
Sbjct: 26 HKLERKWSFW-FDNQSKKGAAWGASLRKAYTFDTVEDFWGLHETIFQTSKLTANAEIHLF 84
Query: 331 KKGIQPTWEDPINEKGGRWI-ICESKSARNXXXXXXXXXXXMIGENFEHPDLICGVVVNI 507
K G++P WEDP GG+W + + +IGE F+ D ICGVV ++
Sbjct: 85 KAGVEPKWEDPECANGGKWTWVVTANRKEALDKGWLETLMALIGEQFDEADEICGVVASV 144
Query: 508 KAKS---KISLWTRGEDKQK-NMKIGKTVKEKLNINRNISYFNHNTTSAKYF 651
+ +S K+SLWTR + + M IGK KE L++ I++ NH+ + F
Sbjct: 145 RPQSKQDKLSLWTRTKSNEAVLMGIGKKWKEILDVTDKITFNNHDDSRRSRF 196
>At1g29550.1 68414.m03614 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 240
Score = 107 bits (256), Expect = 9e-24
Identities = 55/162 (33%), Positives = 85/162 (52%), Gaps = 6/162 (3%)
Frame = +1
Query: 160 HPLEHTWSYWLYTNKSKE----WIHNLVELSTFATVEDYWCLYHYMKLPSELNHGQDYMI 327
H +++W++W SK W +L L TF T+E++W LY+ + P++ G D
Sbjct: 65 HCFQNSWTFWFDNPSSKSNQVIWGSSLRSLYTFGTIEEFWSLYNNIHPPTKWVSGADLYC 124
Query: 328 FKKGIQPTWEDPINEKGGRWIICESKSARNXXXXXXXXXXXMIGENFEHPDLICGVVVNI 507
FK I+P WEDPI GG+W + K+ ++GE F+ D ICG V+N
Sbjct: 125 FKDKIEPKWEDPICANGGKWSMMFPKA--TLECNWLNTLLALVGEQFDQGDEICGAVLNF 182
Query: 508 KAK-SKISLWTR-GEDKQKNMKIGKTVKEKLNINRNISYFNH 627
+A+ +ISLWT+ +++ + IGK KE L N I + H
Sbjct: 183 RARGDRISLWTKNAANEEAQLSIGKQWKELLGYNETIGFIVH 224
>At1g29590.1 68414.m03618 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 285
Score = 105 bits (252), Expect = 3e-23
Identities = 54/162 (33%), Positives = 85/162 (52%), Gaps = 6/162 (3%)
Frame = +1
Query: 160 HPLEHTWSYWLYTNKSKE----WIHNLVELSTFATVEDYWCLYHYMKLPSELNHGQDYMI 327
H +++W++W SK W +L L TFAT+E++W LY+ + P++ G D
Sbjct: 110 HCFQNSWTFWFDNPSSKSNQVIWGSSLRSLYTFATIEEFWSLYNNIHPPTKWVSGSDLYC 169
Query: 328 FKKGIQPTWEDPINEKGGRWIICESKSARNXXXXXXXXXXXMIGENFEHPDLICGVVVNI 507
FK I+P WEDPI GG+W + ++ ++GE F+ D ICG V+N
Sbjct: 170 FKDKIEPKWEDPICANGGKWTMFFPRA--TLESNWLNTLLALVGEQFDQGDEICGAVLNF 227
Query: 508 KAK-SKISLWT-RGEDKQKNMKIGKTVKEKLNINRNISYFNH 627
+ + +ISLWT + +++ + IGK KE L N I + H
Sbjct: 228 RTRGDRISLWTKKAANEEAQLSIGKQWKELLGYNDTIGFIVH 269
>At5g35620.2 68418.m04252 eukaryotic translation initiation factor
4E 2 / eIF-4E2 / mRNA cap-binding protein 2 (EIF4E2)
identical to SP|O04663 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-(iso)4F 25 kDa subunit) (eIF-(ISO)4F P28 subunit)
(eIF4Eiso protein) {Arabidopsis thaliana}
Length = 167
Score = 93.9 bits (223), Expect = 9e-20
Identities = 49/139 (35%), Positives = 75/139 (53%), Gaps = 7/139 (5%)
Frame = +1
Query: 160 HPLEHTWSYWLYTNKSKE---WIHNLVELSTFATVEDYWCLYHYMKLPSELNHGQDYMIF 330
H LE WS+W + N+SK+ W +L + TF TVED+W L+ + S+L + +F
Sbjct: 26 HKLERKWSFW-FDNQSKKGAAWGASLRKAYTFDTVEDFWGLHETIFQTSKLTANAEIHLF 84
Query: 331 KKGIQPTWEDPINEKGGRWI-ICESKSARNXXXXXXXXXXXMIGENFEHPDLICGVVVNI 507
K G++P WEDP GG+W + + +IGE F+ D ICGVV ++
Sbjct: 85 KAGVEPKWEDPECANGGKWTWVVTANRKEALDKGWLETLMALIGEQFDEADEICGVVASV 144
Query: 508 KAKS---KISLWTRGEDKQ 555
+ +S K+SLWTR + +
Sbjct: 145 RPQSKQDKLSLWTRTKSNE 163
>At5g18110.1 68418.m02126 novel cap-binding protein (nCBP) identical
to novel cap-binding protein nCBP [Arabidopsis thaliana]
GI:3108209; contains Pfam profile PF01652: Eukaryotic
initiation factor 4E
Length = 221
Score = 82.6 bits (195), Expect = 2e-16
Identities = 55/182 (30%), Positives = 87/182 (47%), Gaps = 9/182 (4%)
Frame = +1
Query: 82 MSALSSQHETRDGDTSLQSESSPAIKHPLEHTWSYWLYTNKS-----KEWIHNLVELSTF 246
M ++ S + T +S HPL + +S W YT ++ + + N+ ++ F
Sbjct: 17 MDSIKSHYVTDSVSEERRSRELKDGDHPLRYKFSIW-YTRRTPGVRNQSYEDNIKKMVEF 75
Query: 247 ATVEDYWCLYHYMKLPSELNHGQDYMIFKKGIQPTWEDPINEKGGRWIICESK--SARNX 420
+TVE +W Y ++ S L D FK GI+P WED N GG+WII SK SAR
Sbjct: 76 STVEGFWACYCHLARSSLLPSPTDLHFFKDGIRPLWEDGANCNGGKWIIRFSKVVSAR-- 133
Query: 421 XXXXXXXXXXMIGENFEHPDLICGVVVNIKAKSK-ISLWTR-GEDKQKNMKIGKTVKEKL 594
++G+ + D ICG V++++ IS+W R D Q M + ++K L
Sbjct: 134 --FWEDLLLALVGDQLDDADNICGAVLSVRFNEDIISVWNRNASDHQAVMGLRDSIKRHL 191
Query: 595 NI 600
+
Sbjct: 192 KL 193
>At3g07770.1 68416.m00947 heat shock protein-related strong
similarity to heat-shock protein [Secale cereale]
GI:556673; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 803
Score = 28.7 bits (61), Expect = 3.7
Identities = 17/54 (31%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Frame = +1
Query: 106 ETRDGDTSLQSESSPAIKHPLEHTWSYWLYTNKSKE-WIHNLVELSTFATVEDY 264
ET+ D Q+E K +E W W TN+++ W+ N E++T E Y
Sbjct: 323 ETKKDDQDDQTEKKKKTKKVVERYWD-WELTNETQPIWLRNPKEVTTAEYNEFY 375
>At2g35340.1 68415.m04333 RNA helicase, putative similar to
ATP-dependent RNA helicase #3 [Homo sapiens] GI:3107913;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1110
Score = 28.3 bits (60), Expect = 4.9
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = -2
Query: 651 KIFRRCSIMIEVTDVSVNIQFLFYCFADLHIFLFV 547
K FR+ S +E D N +FY D H F++V
Sbjct: 141 KRFRKKSEQLEDDDDEANYFDIFYSMTDFHYFIYV 175
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 27.9 bits (59), Expect = 6.5
Identities = 13/48 (27%), Positives = 29/48 (60%)
Frame = +1
Query: 451 MIGENFEHPDLICGVVVNIKAKSKISLWTRGEDKQKNMKIGKTVKEKL 594
++GE PD+ + V+ + +++ + R ++K+K MK+ K KE++
Sbjct: 356 VLGEMGTLPDVFVELEVSYQLLTRL-IGVRTKEKEKQMKVSKLTKEEI 402
>At1g64590.1 68414.m07321 short-chain dehydrogenase/reductase (SDR)
family protein contains INTERPRO family IPR002198
short-chain dehydrogenase/reductase (SDR) superfamily
Length = 334
Score = 27.9 bits (59), Expect = 6.5
Identities = 17/55 (30%), Positives = 29/55 (52%), Gaps = 4/55 (7%)
Frame = -3
Query: 503 LTTTPQIKSGCSKFSPIISRIRSNHRGS-RLRADLL---SQIIQRPPFSLIGSSH 351
+ T+P++++ C K+ + RS+ GS L+A L S ++ PP S SH
Sbjct: 277 VATSPRLRNVCGKYFSDCNEARSSKSGSCNLKAQRLWTASDLLVSPPNSTTNLSH 331
>At4g19510.2 68417.m02870 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1049
Score = 27.5 bits (58), Expect = 8.5
Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 3/55 (5%)
Frame = +1
Query: 262 YWCLYHYMKLPSELNHGQ--DYMIFKKGIQPTWEDPINEKGGRWI-ICESKSARN 417
+W Y Y LPS+ + + D + I+ WED N + RW+ + +SK N
Sbjct: 592 HWQGYPYDCLPSDFDPKELVDLSLRYSHIKQLWEDEKNTESLRWVDLGQSKDLLN 646
>At4g19510.1 68417.m02869 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1049
Score = 27.5 bits (58), Expect = 8.5
Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 3/55 (5%)
Frame = +1
Query: 262 YWCLYHYMKLPSELNHGQ--DYMIFKKGIQPTWEDPINEKGGRWI-ICESKSARN 417
+W Y Y LPS+ + + D + I+ WED N + RW+ + +SK N
Sbjct: 592 HWQGYPYDCLPSDFDPKELVDLSLRYSHIKQLWEDEKNTESLRWVDLGQSKDLLN 646
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,926,841
Number of Sequences: 28952
Number of extensions: 284981
Number of successful extensions: 769
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 743
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 760
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1412971776
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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