Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10n17r
(443 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g21440.1 68415.m02551 RNA recognition motif (RRM)-containing ... 32 0.15
At1g55940.1 68414.m06416 cytochrome P450, putative similar to SP... 31 0.26
At5g24070.1 68418.m02827 peroxidase family protein similar to ca... 29 1.1
At5g63540.1 68418.m07975 expressed protein ; expression support... 29 1.9
At5g54710.1 68418.m06813 ankyrin repeat family protein contains ... 29 1.9
At5g14730.1 68418.m01728 expressed protein 29 1.9
At4g05310.1 68417.m00809 ubiquitin family protein contains INTER... 29 1.9
At3g05330.1 68416.m00581 cyclin family low similarity to microtu... 27 4.3
At5g65460.1 68418.m08232 kinesin motor protein-related contains ... 27 5.7
At4g08840.1 68417.m01453 pumilio/Puf RNA-binding domain-containi... 27 5.7
At3g14810.1 68416.m01871 mechanosensitive ion channel domain-con... 27 5.7
At2g22720.3 68415.m02692 expressed protein 27 5.7
At2g22720.2 68415.m02691 expressed protein 27 5.7
At2g22720.1 68415.m02693 expressed protein 27 5.7
At1g32030.1 68414.m03940 expressed protein contains Pfam profile... 27 5.7
At1g21650.1 68414.m02710 preprotein translocase secA family prot... 27 7.5
At2g48040.1 68415.m06013 expressed protein 26 10.0
At2g32480.2 68415.m03969 membrane-associated zinc metalloproteas... 26 10.0
At2g32480.1 68415.m03968 membrane-associated zinc metalloproteas... 26 10.0
At1g28600.1 68414.m03522 lipase, putative similar to lipase [Ara... 26 10.0
At1g05990.1 68414.m00627 calcium-binding protein, putative stron... 26 10.0
>At2g21440.1 68415.m02551 RNA recognition motif (RRM)-containing
protein contains InterPro entry IPR000504: RNA-binding
region RNP-1 (RNA recognition motif) (RRM)
Length = 1003
Score = 32.3 bits (70), Expect = 0.15
Identities = 25/79 (31%), Positives = 38/79 (48%), Gaps = 6/79 (7%)
Frame = -1
Query: 257 TVDSNGTKETVFESGVPFDETIDGVLTIKTTYTVDG-----NTVTH-VVENPNGTATFKR 96
T D++ + T+F +PFD T + +K +TV G + V H V + P GTA K
Sbjct: 553 TQDNDDFERTLFIRNLPFDVTKE---EVKQRFTVFGEVESLSLVLHKVTKRPEGTAFVKF 609
Query: 95 EYGDTELKVTISADKWDGV 39
+ D + +AD GV
Sbjct: 610 KTADASVAAISAADTASGV 628
>At1g55940.1 68414.m06416 cytochrome P450, putative similar to
SP:Q42569 from [Arabidopsis thaliana]
Length = 639
Score = 31.5 bits (68), Expect = 0.26
Identities = 19/59 (32%), Positives = 30/59 (50%)
Frame = -1
Query: 185 VLTIKTTYTVDGNTVTHVVENPNGTATFKREYGDTELKVTISADKWDGVAYRYYKV*IN 9
+ + +TT T+ TV + ENP KRE+ E + ADK GV ++ Y+ +N
Sbjct: 435 IASYETTSTMTALTVKFIAENPKVLMELKREH---ETILQNRADKESGVTWKEYRSMMN 490
>At5g24070.1 68418.m02827 peroxidase family protein similar to
cationic peroxidase, Peanut [Arachis hypogaea]
GP|166475|gb|AAA32676; contains Pfam profile PF00141:
Peroxidase
Length = 340
Score = 29.5 bits (63), Expect = 1.1
Identities = 19/70 (27%), Positives = 30/70 (42%)
Frame = +1
Query: 229 VSFVPLLSTVMVLYLSPSLINVVVGLNCKSFRIWSSGRPTNLRNPSKFSSLSNVYFLPKK 408
V+F PLL+ V++ + + GLN +W + TN + ++ V K
Sbjct: 4 VNFFPLLALVVISLAGKATVEAATGLNPPVKLVWHYYKLTNTCDDAETYIRYQVEKFYKN 63
Query: 409 DMVALPKLKR 438
D PKL R
Sbjct: 64 DSSIAPKLLR 73
>At5g63540.1 68418.m07975 expressed protein ; expression supported
by MPSS
Length = 602
Score = 28.7 bits (61), Expect = 1.9
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = -3
Query: 180 DDKNHVHS*WKHRNSCCRESQWHSNIQKGIR*HRTKSYYLGR*M 49
D NHVH H NS + H+ +GI H T+++ R M
Sbjct: 266 DTGNHVHDTGNHVNSSASGNASHTQANQGIPVHVTRTHNSSRAM 309
>At5g54710.1 68418.m06813 ankyrin repeat family protein contains
ankyrin repeat domains, Pfam:PF00023
Length = 598
Score = 28.7 bits (61), Expect = 1.9
Identities = 19/79 (24%), Positives = 36/79 (45%), Gaps = 8/79 (10%)
Frame = -1
Query: 263 TITVDSNGTKETVFESGVPFDETIDGVLTIKTT------YTVDG--NTVTHVVENPNGTA 108
+ + + GT ETVF + +T + ++ Y++D NTV HV + + T+
Sbjct: 259 SFNITTQGTIETVFHLAAKYQKTKAFIFMAQSANIRQLLYSLDAEDNTVLHVAASVDSTS 318
Query: 107 TFKREYGDTELKVTISADK 51
+ +T + VT+ K
Sbjct: 319 LVRHILSETTIDVTLKNKK 337
>At5g14730.1 68418.m01728 expressed protein
Length = 246
Score = 28.7 bits (61), Expect = 1.9
Identities = 14/43 (32%), Positives = 24/43 (55%)
Frame = -1
Query: 290 LIKEGDKYKTITVDSNGTKETVFESGVPFDETIDGVLTIKTTY 162
+I+E + Y ++ VD KE FE+ DE++ T+ TT+
Sbjct: 32 VIRESESYYSVKVDGENDKEFEFETLPLGDESLFHFSTMTTTF 74
>At4g05310.1 68417.m00809 ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 415
Score = 28.7 bits (61), Expect = 1.9
Identities = 17/42 (40%), Positives = 26/42 (61%), Gaps = 3/42 (7%)
Frame = -1
Query: 341 LPEDQIRKLLQFKPTTT--LIKEGDKYKT-ITVDSNGTKETV 225
L E++IRK+ ++P TT + K D ++T + SN KETV
Sbjct: 111 LTEEEIRKIYSYRPETTQDISKIQDAHQTEESPPSNSVKETV 152
>At3g05330.1 68416.m00581 cyclin family low similarity to
microtubule-binding protein TANGLED1 [Zea mays]
GI:11228986; contains Pfam profile PF00134: Cyclin,
N-terminal domain
Length = 444
Score = 27.5 bits (58), Expect = 4.3
Identities = 24/72 (33%), Positives = 37/72 (51%), Gaps = 5/72 (6%)
Frame = -2
Query: 397 KNTHSIEKKTSMG----SLSLSVSPKTRSGNFCSSSLQLH*S-RKATNTRPSPSTATVQR 233
+N ++ +K+S+ +L +SP TR+GNFC S + S + N STA R
Sbjct: 365 RNGSNLARKSSISPKRVTLQAFLSP-TRNGNFCKKSPKASISPTRVCNKSQKLSTAAKFR 423
Query: 232 RQFLSPVCLSTK 197
R F SP L+ +
Sbjct: 424 RSF-SPSRLAMR 434
>At5g65460.1 68418.m08232 kinesin motor protein-related contains
similarity to kinesin heavy chain
Length = 1281
Score = 27.1 bits (57), Expect = 5.7
Identities = 22/105 (20%), Positives = 43/105 (40%), Gaps = 5/105 (4%)
Frame = -1
Query: 353 KFVGLPEDQIRKLLQFKPTTT-----LIKEGDKYKTITVDSNGTKETVFESGVPFDETID 189
+++G+ D+ RKL Q+ T LI E + + + G + + F++
Sbjct: 95 RYLGVLADKSRKLDQYALETEARISPLINEKKRLFNDLLTTKGNVKVFCRARPLFEDEGP 154
Query: 188 GVLTIKTTYTVDGNTVTHVVENPNGTATFKREYGDTELKVTISAD 54
++ T+ NT + NP F R YG + ++ +D
Sbjct: 155 SIIEFPDNCTIRVNTSDDTLSNPKKEFEFDRVYGPQVGQASLFSD 199
>At4g08840.1 68417.m01453 pumilio/Puf RNA-binding domain-containing
protein contains similarity to RNA binding protein PufA
[Dictyostelium discoideum] gi|5106561|gb|AAD39751
Length = 524
Score = 27.1 bits (57), Expect = 5.7
Identities = 17/46 (36%), Positives = 23/46 (50%)
Frame = +3
Query: 189 IDGFVERHTGLKNCLLCTVAVDGDGLVFVAFLDQCSCRLELQKFPD 326
+DG + ++ L VD G V++ DQ CRL LQKF D
Sbjct: 220 LDGSAKCMKNKEDSLDLASMVDSYGSVYLMAKDQLGCRL-LQKFVD 264
>At3g14810.1 68416.m01871 mechanosensitive ion channel
domain-containing protein / MS ion channel
domain-containing protein contains Pfam profile PF00924:
Mechanosensitive ion channel
Length = 853
Score = 27.1 bits (57), Expect = 5.7
Identities = 14/44 (31%), Positives = 26/44 (59%)
Frame = -2
Query: 325 SGNFCSSSLQLH*SRKATNTRPSPSTATVQRRQFLSPVCLSTKP 194
SG+ S ++L+ + +NT P P+TA+ R+ L+ + S+ P
Sbjct: 101 SGDEISLDVELNMAELQSNTPPRPATASNTPRRGLTTISESSSP 144
>At2g22720.3 68415.m02692 expressed protein
Length = 569
Score = 27.1 bits (57), Expect = 5.7
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = -2
Query: 310 SSSLQLH*SRKATNTRPSPSTATVQRRQFLS 218
SSS + R ATN RP PS ++++R+ +S
Sbjct: 382 SSSNGVGPGRSATNARPLPSKSSLERKPSIS 412
>At2g22720.2 68415.m02691 expressed protein
Length = 672
Score = 27.1 bits (57), Expect = 5.7
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = -2
Query: 310 SSSLQLH*SRKATNTRPSPSTATVQRRQFLS 218
SSS + R ATN RP PS ++++R+ +S
Sbjct: 485 SSSNGVGPGRSATNARPLPSKSSLERKPSIS 515
>At2g22720.1 68415.m02693 expressed protein
Length = 340
Score = 27.1 bits (57), Expect = 5.7
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = -2
Query: 310 SSSLQLH*SRKATNTRPSPSTATVQRRQFLS 218
SSS + R ATN RP PS ++++R+ +S
Sbjct: 153 SSSNGVGPGRSATNARPLPSKSSLERKPSIS 183
>At1g32030.1 68414.m03940 expressed protein contains Pfam profile
PF03754: Domain of unknown function (DUF313); expression
supported by MPSS
Length = 333
Score = 27.1 bits (57), Expect = 5.7
Identities = 13/43 (30%), Positives = 20/43 (46%)
Frame = -1
Query: 170 TTYTVDGNTVTHVVENPNGTATFKREYGDTELKVTISADKWDG 42
+T + NT NPN + KR++ D+E K + W G
Sbjct: 141 STLLAEYNTEMANPPNPNSETSLKRKHSDSEPKKAKTPYSWKG 183
>At1g21650.1 68414.m02710 preprotein translocase secA family protein
contains Pfam profiles: PF01043 SecA protein, amino
terminal region, PF00400 WD domain, G-beta repeat,
PF00097 zinc finger, C3HC4 type (RING finger)
Length = 1579
Score = 26.6 bits (56), Expect = 7.5
Identities = 19/60 (31%), Positives = 27/60 (45%)
Frame = +3
Query: 150 SINCVRGFYRQYTIDGFVERHTGLKNCLLCTVAVDGDGLVFVAFLDQCSCRLELQKFPDL 329
S+NCV G + I+ + +TG+ L + G V L QC C L+ Q P L
Sbjct: 375 SVNCVDGKGCEEGIEDILVLYTGITEKLSLESELQGKFKSMVEILRQCCC-LDPQARPVL 433
>At2g48040.1 68415.m06013 expressed protein
Length = 294
Score = 26.2 bits (55), Expect = 10.0
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = +1
Query: 325 IWSSGRPTNLRNPSKFSSL 381
++SSG PTN+ NP K +S+
Sbjct: 1 MYSSGNPTNIANPIKDASV 19
>At2g32480.2 68415.m03969 membrane-associated zinc metalloprotease,
putative similar to Hypothetical zinc metalloprotease
All3971 (SP:Q8YQ64) [strain PCC 7120] {Anabaena sp.}
Pfam PF00595: PDZ domain (Also known as DHR or GLGF);
contains TIGRFAM TIGR00054: membrane-associated zinc
metalloprotease, putative
Length = 410
Score = 26.2 bits (55), Expect = 10.0
Identities = 15/52 (28%), Positives = 22/52 (42%), Gaps = 1/52 (1%)
Frame = -1
Query: 194 IDGVLTIKTTYTVDGNTVTHVVENPNGTATFKREYGDTELKVTISADK-WDG 42
+DG KT V V NP F+ E G + + ++ DK +DG
Sbjct: 238 VDGTELSKTGPDAVSKIVDIVKRNPKSNVVFRIERGGEDFDIRVTPDKNFDG 289
>At2g32480.1 68415.m03968 membrane-associated zinc metalloprotease,
putative similar to Hypothetical zinc metalloprotease
All3971 (SP:Q8YQ64) [strain PCC 7120] {Anabaena sp.}
Pfam PF00595: PDZ domain (Also known as DHR or GLGF);
contains TIGRFAM TIGR00054: membrane-associated zinc
metalloprotease, putative
Length = 447
Score = 26.2 bits (55), Expect = 10.0
Identities = 15/52 (28%), Positives = 22/52 (42%), Gaps = 1/52 (1%)
Frame = -1
Query: 194 IDGVLTIKTTYTVDGNTVTHVVENPNGTATFKREYGDTELKVTISADK-WDG 42
+DG KT V V NP F+ E G + + ++ DK +DG
Sbjct: 238 VDGTELSKTGPDAVSKIVDIVKRNPKSNVVFRIERGGEDFDIRVTPDKNFDG 289
>At1g28600.1 68414.m03522 lipase, putative similar to lipase
[Arabidopsis thaliana] GI:1145627; contains InterPro
Entry IPR001087 Lipolytic enzyme, G-D-S-L family
Length = 393
Score = 26.2 bits (55), Expect = 10.0
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = +3
Query: 78 FGVTVFPFECCCAIGILYNMSY 143
FG PF CC IG YN ++
Sbjct: 297 FGFMERPFPACCGIGGPYNFNF 318
>At1g05990.1 68414.m00627 calcium-binding protein, putative strong
similarity to calcium-binding protein [Lotus japonicus]
GI:18413495; contains INTERPRO:IPR002048 calcium-binding
EF-hand domain
Length = 150
Score = 26.2 bits (55), Expect = 10.0
Identities = 12/35 (34%), Positives = 21/35 (60%)
Frame = -1
Query: 278 GDKYKTITVDSNGTKETVFESGVPFDETIDGVLTI 174
G+ YKTI + + +E + E+ FD+ DG +T+
Sbjct: 63 GELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITV 97
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,228,276
Number of Sequences: 28952
Number of extensions: 221345
Number of successful extensions: 803
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 785
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 803
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 712739520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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