BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10n16f (639 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_43527| Best HMM Match : Trypsin (HMM E-Value=0) 42 4e-04 SB_42238| Best HMM Match : Trypsin (HMM E-Value=0) 39 0.003 SB_52206| Best HMM Match : No HMM Matches (HMM E-Value=.) 39 0.004 SB_4778| Best HMM Match : No HMM Matches (HMM E-Value=.) 39 0.004 SB_33085| Best HMM Match : Trypsin (HMM E-Value=0) 38 0.009 SB_56478| Best HMM Match : Trypsin (HMM E-Value=0) 35 0.049 SB_13096| Best HMM Match : No HMM Matches (HMM E-Value=.) 35 0.064 SB_35182| Best HMM Match : CUB (HMM E-Value=0) 33 0.20 SB_59555| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.26 SB_15907| Best HMM Match : Aldedh (HMM E-Value=3.2e-23) 33 0.26 SB_50354| Best HMM Match : Trypsin (HMM E-Value=0.0028) 33 0.26 SB_11023| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.26 SB_51536| Best HMM Match : Trypsin (HMM E-Value=0) 32 0.34 SB_42973| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 0.79 SB_48258| Best HMM Match : Kelch_1 (HMM E-Value=0) 28 7.4 SB_43991| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.4 SB_38546| Best HMM Match : Trypsin (HMM E-Value=1.90577e-43) 28 7.4 SB_35220| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.4 SB_22064| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.4 SB_4906| Best HMM Match : Trypsin (HMM E-Value=0) 28 7.4 SB_36971| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 9.7 >SB_43527| Best HMM Match : Trypsin (HMM E-Value=0) Length = 366 Score = 41.9 bits (94), Expect = 4e-04 Identities = 23/100 (23%), Positives = 41/100 (41%), Gaps = 3/100 (3%) Frame = +2 Query: 338 HPEFSEENYDKDVSIVRVTHAIHFGPNXX---XXXXXXXXXXXXXXXXXDLLGWGTTVQG 508 HP +S ++YD D++++R+ + F + GWG Sbjct: 199 HPHYSPDSYDSDIALIRLAQPVTFTDYVKPICLPSAASDYAQLQANVSGTISGWGKRKLW 258 Query: 509 GSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAG 628 LH+ + + + + CR+ + D +VT N FCAG Sbjct: 259 RDRVANRLHEATVPIVDIQTCRKAHP--DYIVTANMFCAG 296 >SB_42238| Best HMM Match : Trypsin (HMM E-Value=0) Length = 657 Score = 39.1 bits (87), Expect = 0.003 Identities = 37/169 (21%), Positives = 64/169 (37%), Gaps = 8/169 (4%) Frame = +2 Query: 146 EVFLPILNQWFQQCAGIVLTNYHYLSTATCF--HGEFYDPAYRRIIAGSSRRSE------ 301 E+ L N C G ++ Y L+++ CF + R+ RR E Sbjct: 2 EIILKTPNLTTHYCGGSLIDPYWILTSSHCFWTYNNISTQFEIRLGEHDERRIEGYEEII 61 Query: 302 PGEISYVHFAVNHPEFSEENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDL 481 G+ Y+H + + D DV+++++ F + Sbjct: 62 QGDQLYIHPGLVVGDLISPG-DYDVALIKLKRPAVFHKRVHSVCLPSVTANLTTGTKCFV 120 Query: 482 LGWGTTVQGGSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAG 628 GWG T +G S L++LE+ + +KE C ++ + D FCAG Sbjct: 121 TGWGKTAEGSPYSP-VLNELEVDIVSKEVCNAN-DSYNGTINDRYFCAG 167 >SB_52206| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 786 Score = 38.7 bits (86), Expect = 0.004 Identities = 33/151 (21%), Positives = 58/151 (38%), Gaps = 3/151 (1%) Frame = +2 Query: 185 CAGIVLTNYHYLSTATCFHGEFYDPAYRRIIAGSSRRSEPG---EISYVHFAVNHPEFSE 355 C G V++ + L+ A C E + ++ G R ++ V ++H +S Sbjct: 574 CGGNVISPWWVLTAAHCVQDE--RASNIKLTMGEWRLFNVDGTEQVIPVERIISHANYSY 631 Query: 356 ENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDLLGWGTTVQGGSVSDGNLH 535 D D +++++T ++F + GWG+T GS S L Sbjct: 632 NTVDYDYALLKLTRPLNF--TQYVQPVCLPDSDFPAGTLCYVTGWGSTNYRGSPSPNYLQ 689 Query: 536 KLELIVTNKENCREQYKGHDRVVTDNKFCAG 628 ++ L + N C Y R +T CAG Sbjct: 690 EVGLPLVNHSQCHATYLTASRKITPRMRCAG 720 >SB_4778| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 867 Score = 38.7 bits (86), Expect = 0.004 Identities = 33/161 (20%), Positives = 65/161 (40%), Gaps = 5/161 (3%) Frame = +2 Query: 161 ILNQWFQQCAGIVLTNYHYLSTATCFHGEFYDPAYRRIIAGSSRRSEPGEISYVHFAV-- 334 I N Q C G ++T ++ A C + +PA ++ G+ R+ P + ++ Sbjct: 649 IYNSGRQFCGGTLVTPEWVITAAHCVVDK--NPASIQVRLGAQDRTSPDPSVEMRISIRS 706 Query: 335 --NHPEF-SEENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDLLGWGTTVQ 505 NHP++ S + D++++R++ + GWGT Sbjct: 707 IHNHPDYGSPKRSSNDIALLRLSRPTILTHRINLACMPNDTVHFPNGTMCYITGWGTLSS 766 Query: 506 GGSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAG 628 GGS + L++ + + + C Y G ++ + CAG Sbjct: 767 GGSQPEA-LNQAVVPLRTRSECERSYPGK---ISADMICAG 803 >SB_33085| Best HMM Match : Trypsin (HMM E-Value=0) Length = 537 Score = 37.5 bits (83), Expect = 0.009 Identities = 23/99 (23%), Positives = 39/99 (39%) Frame = +2 Query: 332 VNHPEFSEENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDLLGWGTTVQGG 511 ++H +S D D +++++T ++F + GWG+T G Sbjct: 375 ISHANYSYNTVDYDYALLKLTRPLNF--TQYVQPVCLPDSDFPAGTLCYVTGWGSTNYRG 432 Query: 512 SVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAG 628 S S L ++ L + N C Y R +T CAG Sbjct: 433 SPSPNYLQEVGLPLVNHSQCHATYLTASRKITPRMRCAG 471 >SB_56478| Best HMM Match : Trypsin (HMM E-Value=0) Length = 968 Score = 35.1 bits (77), Expect = 0.049 Identities = 28/154 (18%), Positives = 60/154 (38%), Gaps = 5/154 (3%) Frame = +2 Query: 185 CAGIVLTNYHYLSTATCFHGEFYDPAYRRIIAGSSRRSEPGEISYVHFA---VNHPEFSE 355 C G +++ ++ A C G + P+Y +++ G R+ P H + HP ++ Sbjct: 93 CGGSLVSPTWVVTAAHCIAGSSHTPSY-KVVTGEHIRNSPEGTEQTHDVKRIITHPTYNS 151 Query: 356 ENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDLLGWGTTVQGGSVSDGNLH 535 D++++ ++ + + GWG G S L Sbjct: 152 PQLSNDIALIELSSPVPLSDRGHQVSVGSKCF---------ITGWGKIRHPGG-SHHILQ 201 Query: 536 KLELIVTNKENCRE--QYKGHDRVVTDNKFCAGL 631 + + +++ C++ Q G +TD+ CAG+ Sbjct: 202 QAMMPPLSQDACKKKVQQAGFGIQITDSMVCAGV 235 >SB_13096| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1465 Score = 34.7 bits (76), Expect = 0.064 Identities = 24/100 (24%), Positives = 37/100 (37%), Gaps = 2/100 (2%) Frame = +2 Query: 335 NHPEFSEENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXD--LLGWGTTVQG 508 +HP FS YD D++++R+ + +LGWG T Sbjct: 1249 SHPRFSWRTYDSDIALIRLRWKANITEYVRPVCLPNGYQRRLVKPRAKGVMLGWGVTEN- 1307 Query: 509 GSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAG 628 L + + V N C++ Y+ VT N CAG Sbjct: 1308 -KRPSKTLKEAIVPVVNHNACKKAYENETWPVTSNMLCAG 1346 >SB_35182| Best HMM Match : CUB (HMM E-Value=0) Length = 963 Score = 33.1 bits (72), Expect = 0.20 Identities = 14/49 (28%), Positives = 26/49 (53%) Frame = +2 Query: 485 GWGTTVQGGSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAGL 631 GWG +G + L + ++ + +++ C Q DR +T+N CAG+ Sbjct: 351 GWGALQEGAGSTSKVLMQAKVPLVSRDQCSHQQSYGDR-ITENMLCAGM 398 >SB_59555| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 898 Score = 32.7 bits (71), Expect = 0.26 Identities = 28/150 (18%), Positives = 54/150 (36%), Gaps = 2/150 (1%) Frame = +2 Query: 185 CAGIVLTNYHYLSTATCFHGEFYDPAYRRIIAGSSRRSE-PGEISY-VHFAVNHPEFSEE 358 C G ++ ++ A C Y ++ R P + ++ + H +FS Sbjct: 57 CGGSLIAKDWVVTAAHCVDRNPTPSGYTVVVGAHHRTGNTPVQNTFRLKQLFKHEQFSMR 116 Query: 359 NYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDLLGWGTTVQGGSVSDGNLHK 538 + D++++++ + + GWG TV GGS + G L + Sbjct: 117 HLKNDIALLQLHEPVKASDKVNTVCLPSSGSRAQAGARCYITGWGRTVGGGSAA-GILQQ 175 Query: 539 LELIVTNKENCREQYKGHDRVVTDNKFCAG 628 L V +C+ V + + CAG Sbjct: 176 AMLPVAGDSDCQRTNGRLVPVDSRSMICAG 205 >SB_15907| Best HMM Match : Aldedh (HMM E-Value=3.2e-23) Length = 275 Score = 32.7 bits (71), Expect = 0.26 Identities = 18/56 (32%), Positives = 30/56 (53%), Gaps = 1/56 (1%) Frame = -3 Query: 340 MVNSK-VNIRYFTGLTATGRSSDNATVCRIVEFSMETGSS*KVVVVGENNTSALLE 176 M+NS+ +N FTG TGR AT +V+ +E GS ++V+ + + +E Sbjct: 215 MINSRGINALTFTGSLETGRKVAAATAVNLVKCQLEMGSKNALIVLDDADLDNAVE 270 >SB_50354| Best HMM Match : Trypsin (HMM E-Value=0.0028) Length = 333 Score = 32.7 bits (71), Expect = 0.26 Identities = 20/66 (30%), Positives = 32/66 (48%), Gaps = 3/66 (4%) Frame = +2 Query: 146 EVFLPILNQW--FQQCAGIVLTNYHYLSTATCFH-GEFYDPAYRRIIAGSSRRSEPGEIS 316 E F+ + W QC+G ++ H ++ A C H G+++ I+ G S S E+ Sbjct: 138 EPFVSSVKLWSDVMQCSGTLIGPCHVITAAHCIHNGKWFVSPLTAILVGVSNTSSTDELD 197 Query: 317 YVHFAV 334 Y FAV Sbjct: 198 Y--FAV 201 >SB_11023| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 669 Score = 32.7 bits (71), Expect = 0.26 Identities = 28/162 (17%), Positives = 59/162 (36%), Gaps = 8/162 (4%) Frame = +2 Query: 176 FQQCAGIVLTNYHYLSTATCFH-----GEFYDPAYRRIIAGSSRRSEPGEISY---VHFA 331 F C G ++++ ++ A H G +P ++ G+ ++ + + Sbjct: 452 FHTCGGSLISDRWVVTAAQGIHRNKNPGGRQNPGGYTVVVGAHKKRGSTSVQQSLRLSQI 511 Query: 332 VNHPEFSEENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDLLGWGTTVQGG 511 + HP++++ D++++ + + F + GWG Sbjct: 512 IEHPKYNDRRIVNDIALLELATPVQFDSAGKVGTVCLTDQRPAPGKRCYITGWGAINGDT 571 Query: 512 SVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAGLVR 637 S L + L + + ENCR +Y V + CAG R Sbjct: 572 QQSPDILQQAMLPIASHENCRRKY---GDVSSTAHLCAGEAR 610 Score = 30.7 bits (66), Expect = 1.0 Identities = 12/80 (15%), Positives = 34/80 (42%), Gaps = 2/80 (2%) Frame = +2 Query: 176 FQQCAGIVLTNYHYLSTATCFHGEFYDPAYRRIIAGSSRRSEPGEISYVHFA--VNHPEF 349 F C G ++++ ++ A C H Y ++ +R + + + HP++ Sbjct: 129 FHTCGGSLISDRWVVTAAHCIHRNKNPGGYTVVVGAHKKRGSTSVQQSLRLSQIIEHPKY 188 Query: 350 SEENYDKDVSIVRVTHAIHF 409 ++ D++++ + + F Sbjct: 189 NDRRIVNDIALLELATPVQF 208 >SB_51536| Best HMM Match : Trypsin (HMM E-Value=0) Length = 347 Score = 32.3 bits (70), Expect = 0.34 Identities = 27/151 (17%), Positives = 56/151 (37%), Gaps = 3/151 (1%) Frame = +2 Query: 185 CAGIVLTNYHYLSTATCFHGEFYDPAYRRIIAGSSRRSEPG--EISYVHFAVNHPEFS-E 355 C G V+ ++ A CF Y + + G + V + HP+++ Sbjct: 135 CGGSVVAPEWIVTAAHCFAYSKDAKDYTIAVGEHDLNATDGYEQRPDVERIILHPKYAPH 194 Query: 356 ENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXXXXXDLLGWGTTVQGGSVSDGNLH 535 N+D DV+++++ + + + GWG + G LH Sbjct: 195 NNHDYDVALIKLASPLQYNDRVRPVCLPSLKEDLEENTQCYISGWGHLQEAGH-GPWVLH 253 Query: 536 KLELIVTNKENCREQYKGHDRVVTDNKFCAG 628 + + + +++ C++ Y V+ CAG Sbjct: 254 QAAVPLVSRDTCQKAYNDLHYKVSSRMRCAG 284 >SB_42973| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1864 Score = 31.1 bits (67), Expect = 0.79 Identities = 20/91 (21%), Positives = 41/91 (45%), Gaps = 3/91 (3%) Frame = +2 Query: 146 EVFLPILNQWFQQCAGIVLTNYHYLSTATCFHGEFYDPAYRRIIAGSS---RRSEPGEIS 316 +V L + Q+C G ++ L+ A CF F P + G S RR G+ Sbjct: 1253 QVALTLSGNPVQRCGGALIAADWVLTAAHCF-DRFSMPPEWTVQVGVSDMRRRDGKGQSL 1311 Query: 317 YVHFAVNHPEFSEENYDKDVSIVRVTHAIHF 409 + HP+++ +++ D++++++ F Sbjct: 1312 SIKAIFVHPQYNSSSHEHDLAVLQLQQPAEF 1342 >SB_48258| Best HMM Match : Kelch_1 (HMM E-Value=0) Length = 473 Score = 27.9 bits (59), Expect = 7.4 Identities = 14/41 (34%), Positives = 22/41 (53%) Frame = +2 Query: 131 SLVQIEVFLPILNQWFQQCAGIVLTNYHYLSTATCFHGEFY 253 +LV +E + P N+W ++ +LT Y STA G+ Y Sbjct: 111 NLVSMERYDPSTNEWEEEAVAPMLTARKYFSTAV-LDGKLY 150 >SB_43991| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1149 Score = 27.9 bits (59), Expect = 7.4 Identities = 18/71 (25%), Positives = 33/71 (46%), Gaps = 1/71 (1%) Frame = -3 Query: 535 VEVAVRYTAALNGSSPSEQI-NKNTLGYYDTLLDNSTLLDVWAEVDGMCYSYDAHILVVI 359 +++ V Y AA+ S Q N+N L Y + D + A + M SY+ + + Sbjct: 303 MKIKVNYVAAVQDESTYSQFYNENNLKYEEEFTDVLENIKTRARIVIMVTSYNMAVEGMH 362 Query: 358 LLREFGMVNSK 326 L + G++N + Sbjct: 363 LAKNRGLINGE 373 >SB_38546| Best HMM Match : Trypsin (HMM E-Value=1.90577e-43) Length = 506 Score = 27.9 bits (59), Expect = 7.4 Identities = 14/51 (27%), Positives = 23/51 (45%) Frame = +2 Query: 479 LLGWGTTVQGGSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAGL 631 + GWGT GG+ L + + + ++ C Y + D+ CAGL Sbjct: 78 ITGWGTLASGGN-QPNKLMQASVPLVSQSKCNTAYPNK---IHDSMLCAGL 124 >SB_35220| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 166 Score = 27.9 bits (59), Expect = 7.4 Identities = 20/77 (25%), Positives = 37/77 (48%) Frame = -3 Query: 592 MTLVLFPAVFFVGHNEFELVEVAVRYTAALNGSSPSEQINKNTLGYYDTLLDNSTLLDVW 413 M +VLF A+F + EF ++ + + T + +GS + +N Y D +D T+L Sbjct: 1 MAVVLF-ALFRL--LEFLMLVIFTKGTCSNSGSQQALHVNITCEKYLDVYVDGETMLTGV 57 Query: 412 AEVDGMCYSYDAHILVV 362 V + +H++ V Sbjct: 58 QGVHSILIDSSSHVMAV 74 >SB_22064| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 398 Score = 27.9 bits (59), Expect = 7.4 Identities = 15/51 (29%), Positives = 25/51 (49%) Frame = +3 Query: 201 SPTTTTFQLLPVSMENSTILHTVALSLDLPVAVSPVKYLMFTLLLTIPNSL 353 SP+T+ Q++P + + L T + S +LPV + FT IP + Sbjct: 143 SPSTSVKQMVPNTQASRLCLQTTSASSNLPVTTQQSFRMTFTPPSMIPKPI 193 >SB_4906| Best HMM Match : Trypsin (HMM E-Value=0) Length = 530 Score = 27.9 bits (59), Expect = 7.4 Identities = 14/51 (27%), Positives = 23/51 (45%) Frame = +2 Query: 479 LLGWGTTVQGGSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAGL 631 + GWGT GG+ L + + + ++ C Y + D+ CAGL Sbjct: 422 ITGWGTLASGGN-QPNKLMQASVPLVSQSKCNTAYPNK---IHDSMLCAGL 468 >SB_36971| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1870 Score = 27.5 bits (58), Expect = 9.7 Identities = 25/117 (21%), Positives = 43/117 (36%), Gaps = 4/117 (3%) Frame = +2 Query: 290 RRSEPGEISY-VHFAVNHPEFSEENYDKDVSIVRVTHAIHFGPNXXXXXXXXXXXXXXXX 466 RR + E + V + HP + + D DV+++R++H Sbjct: 1394 RRGDGTEQDFTVRQVIVHPNYRRQTTDSDVALLRLSHPATLNKAVSLICLPKEGESEAVG 1453 Query: 467 XXXDLLGWGTT---VQGGSVSDGNLHKLELIVTNKENCREQYKGHDRVVTDNKFCAG 628 + GWG T G SV L + + + +++ C + VVT C G Sbjct: 1454 KNCYITGWGRTGYNRPGASV----LQEAMMPIVSQQTCAAAMRRWG-VVTSRMICGG 1505 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 21,759,024 Number of Sequences: 59808 Number of extensions: 505432 Number of successful extensions: 1241 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 1112 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1239 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1608851125 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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