BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10n03f (626 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase... 28 1.3 SPAC20G8.09c |||N-acetyltransferase Nat10 |Schizosaccharomyces p... 26 3.9 SPAC10F6.15 |||S. pombe specific UPF0300 family protein 1|Schizo... 26 3.9 SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 26 3.9 SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|... 26 5.1 SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 25 6.8 SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 25 6.8 SPAC6G10.09 |||glucosidase I Gls1 |Schizosaccharomyces pombe|chr... 25 9.0 SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 25 9.0 SPAC1B3.10c |||SEL1 repeat protein, unknown biological role|Schi... 25 9.0 >SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase Tdh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 336 Score = 27.9 bits (59), Expect = 1.3 Identities = 24/102 (23%), Positives = 43/102 (42%), Gaps = 4/102 (3%) Frame = +2 Query: 239 MDYDVVEGKIPRDLLEYDFAEDAMDFECQLAESQDVNFTQ-TLSEPLTIGSHIICTGTPS 415 +D + ++ RD + ++ D+ + ES V TQ T S L G+ + PS Sbjct: 69 IDGNAIDVHNERDPADIKWSTSGADY---VIESTGVFTTQETASAHLKGGAKRVIISAPS 125 Query: 416 EDIPWFAINIGSEELEAGR---SDISVHFNVRQPQCYVVRNT 532 +D P + + + E+ S+ S N P V+ +T Sbjct: 126 KDAPMYVVGVNEEKFNPSEKVISNASCTTNCLAPLAKVINDT 167 >SPAC20G8.09c |||N-acetyltransferase Nat10 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1033 Score = 26.2 bits (55), Expect = 3.9 Identities = 12/34 (35%), Positives = 19/34 (55%) Frame = +2 Query: 149 QQFFNPSNTHLKKSKNFAKKIMKCLNCVSKMDYD 250 ++F PSN L +KKIMKC++ + D + Sbjct: 885 KEFNLPSNQLLAMLVKLSKKIMKCIDEIETKDIE 918 >SPAC10F6.15 |||S. pombe specific UPF0300 family protein 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 432 Score = 26.2 bits (55), Expect = 3.9 Identities = 16/37 (43%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Frame = -2 Query: 256 YNV-VIHFTNTIKAFHYFFCKIFRFLQMCIRGIKKLL 149 YN V+ F +TI H F KI +FL +C+R K+ L Sbjct: 3 YNANVVKFHSTI--LHKFALKISKFLILCMRRNKRQL 37 >SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 188 Score = 26.2 bits (55), Expect = 3.9 Identities = 12/25 (48%), Positives = 14/25 (56%) Frame = -2 Query: 241 HFTNTIKAFHYFFCKIFRFLQMCIR 167 H T TI+AF Y+F F F C R Sbjct: 66 HHTFTIEAFFYWFFFFFFFFSHCRR 90 >SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|Schizosaccharomyces pombe|chr 2|||Manual Length = 335 Score = 25.8 bits (54), Expect = 5.1 Identities = 16/62 (25%), Positives = 26/62 (41%), Gaps = 3/62 (4%) Frame = +2 Query: 356 QTLSEPLTIGSHIICTGTPSEDIPWFAINIGSEELEAGR---SDISVHFNVRQPQCYVVR 526 +T S L G+ + PS+D P F + + E+ S+ S N P V+ Sbjct: 106 ETASAHLKGGAKRVIISAPSKDAPMFVVGVNLEKFNPSEKVISNASCTTNCLAPLAKVIN 165 Query: 527 NT 532 +T Sbjct: 166 DT 167 >SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 2685 Score = 25.4 bits (53), Expect = 6.8 Identities = 11/33 (33%), Positives = 18/33 (54%) Frame = -2 Query: 316 KIHCVLCEIVF*KIPRDFTFYNVVIHFTNTIKA 218 K+H V C + +IP F + ++ NT+KA Sbjct: 849 KLHQVTCSYISIRIPHKFIPFILIESAINTVKA 881 >SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces pombe|chr 2|||Manual Length = 262 Score = 25.4 bits (53), Expect = 6.8 Identities = 15/50 (30%), Positives = 24/50 (48%) Frame = -3 Query: 495 LKCTEMSERPASNSSLPMFIANHGMSSLGVPVQIICEPIVKGSDKVCVKF 346 L+ TE+ +P + P + S P+Q +PIV GS + +KF Sbjct: 4 LELTEVWGKPQKDIFFPS--GSEVEESTDAPIQRTVQPIVTGSSVLALKF 51 >SPAC6G10.09 |||glucosidase I Gls1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 808 Score = 25.0 bits (52), Expect = 9.0 Identities = 9/14 (64%), Positives = 11/14 (78%) Frame = -3 Query: 579 SLYAVVSSGPHFPR 538 SL+ +V S PHFPR Sbjct: 394 SLFTIVPSRPHFPR 407 >SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosaccharomyces pombe|chr 2|||Manual Length = 502 Score = 25.0 bits (52), Expect = 9.0 Identities = 11/39 (28%), Positives = 22/39 (56%) Frame = -2 Query: 259 FYNVVIHFTNTIKAFHYFFCKIFRFLQMCIRGIKKLLVL 143 ++NVVIH +K F +F + + C + +++L +L Sbjct: 188 YFNVVIHMLAHVKPFRNYF--LLKNFDNCPQLVQRLAIL 224 >SPAC1B3.10c |||SEL1 repeat protein, unknown biological role|Schizosaccharomyces pombe|chr 1|||Manual Length = 680 Score = 25.0 bits (52), Expect = 9.0 Identities = 9/15 (60%), Positives = 13/15 (86%) Frame = -2 Query: 253 NVVIHFTNTIKAFHY 209 N+ IHF+NTIKA ++ Sbjct: 666 NIPIHFSNTIKAANF 680 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,469,935 Number of Sequences: 5004 Number of extensions: 49278 Number of successful extensions: 127 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 122 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 127 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 277683324 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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