BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10n01f (612 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB8E5.06c |rpl302|rpl3-2, rpl3-b|60S ribosomal protein L3|Sch... 273 1e-74 SPAC17A5.03 |rpl301|rpl3-1, rpl3|60S ribosomal protein L3 |Schiz... 272 2e-74 SPBC1683.12 |||nicotinic acid plasma membrane transporter |Schiz... 26 3.7 SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFII... 26 3.7 SPCC794.03 |||amino acid permease, unknown 13|Schizosaccharomyce... 26 4.9 SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ... 25 6.5 SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1||... 25 8.6 >SPAPB8E5.06c |rpl302|rpl3-2, rpl3-b|60S ribosomal protein L3|Schizosaccharomyces pombe|chr 1|||Manual Length = 388 Score = 273 bits (669), Expect = 1e-74 Identities = 123/195 (63%), Positives = 156/195 (80%) Frame = +1 Query: 28 MSHRKFSAPRHGSMGFYPKKRSRRHRGKVKAFPKDDPSKPVHLTAFIGYKAGMTHVVREP 207 MSH KF PRHGS+GF P+KR+ R RGKVKAFPKDD SKPVHLTAF+GYKAGMTH+VR+ Sbjct: 1 MSHCKFEQPRHGSLGFLPRKRASRQRGKVKAFPKDDASKPVHLTAFLGYKAGMTHIVRDL 60 Query: 208 DRPGSKINKKEIVEAVTIIETPPMVCVGVVGYIETPHGLRALLTVWAEHMSEDCRRRFYK 387 DRPGSK++K+EI+EAVTIIETPPMV VGVVGY+ETP GLR+L TVWAEH+SE+ +RRFYK Sbjct: 61 DRPGSKMHKREILEAVTIIETPPMVVVGVVGYVETPRGLRSLTTVWAEHLSEEVKRRFYK 120 Query: 388 NWYXXXXXXXXXXXXXWQDELGRKSIEKDFKKMIRYCSVVRVIAHTQMKLLKQRQKKAHI 567 NW+ + + +SI ++ +++ +YCSVVRV+AHTQ++ QKKAH+ Sbjct: 121 NWFKSKKKAFTKYAKKYAE--STQSINRELERIKKYCSVVRVLAHTQIRKTPLAQKKAHL 178 Query: 568 MEIQLNGGTIEDKVK 612 MEIQ+NGG++ DKV+ Sbjct: 179 MEIQVNGGSVADKVE 193 >SPAC17A5.03 |rpl301|rpl3-1, rpl3|60S ribosomal protein L3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 388 Score = 272 bits (668), Expect = 2e-74 Identities = 122/195 (62%), Positives = 156/195 (80%) Frame = +1 Query: 28 MSHRKFSAPRHGSMGFYPKKRSRRHRGKVKAFPKDDPSKPVHLTAFIGYKAGMTHVVREP 207 MSH KF PRHGS+GF P+KR+ R RGKVKAFPKDD SKPVHLTAF+GYKAGMTH+VR+ Sbjct: 1 MSHCKFEQPRHGSLGFLPRKRASRQRGKVKAFPKDDASKPVHLTAFLGYKAGMTHIVRDL 60 Query: 208 DRPGSKINKKEIVEAVTIIETPPMVCVGVVGYIETPHGLRALLTVWAEHMSEDCRRRFYK 387 DRPGSK++K+EI+EAVT+IETPPMV VGVVGY+ETP GLR+L TVWAEH+SE+ +RRFYK Sbjct: 61 DRPGSKMHKREILEAVTVIETPPMVVVGVVGYVETPRGLRSLTTVWAEHLSEEVKRRFYK 120 Query: 388 NWYXXXXXXXXXXXXXWQDELGRKSIEKDFKKMIRYCSVVRVIAHTQMKLLKQRQKKAHI 567 NW+ + + +SI ++ +++ +YCSVVRV+AHTQ++ QKKAH+ Sbjct: 121 NWFKSKKKAFTKYAKKYAE--STQSINRELERIKKYCSVVRVLAHTQIRKTPLAQKKAHL 178 Query: 568 MEIQLNGGTIEDKVK 612 MEIQ+NGG++ DKV+ Sbjct: 179 MEIQVNGGSVADKVE 193 >SPBC1683.12 |||nicotinic acid plasma membrane transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 482 Score = 26.2 bits (55), Expect = 3.7 Identities = 14/37 (37%), Positives = 21/37 (56%) Frame = +1 Query: 496 CSVVRVIAHTQMKLLKQRQKKAHIMEIQLNGGTIEDK 606 C+VV + T M+ QRQ K I + LNG +++K Sbjct: 433 CTVVGTLTATAMRFYLQRQNK--IKQEILNGERVDEK 467 >SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFIID complex subunit Taf111|Schizosaccharomyces pombe|chr 1|||Manual Length = 979 Score = 26.2 bits (55), Expect = 3.7 Identities = 18/51 (35%), Positives = 25/51 (49%) Frame = +2 Query: 302 TLRPLMDYALC*LSGRSICLKTVDVASTKTGTNARRRLSLKPVRNGRMSLD 454 TL DYA+ S +S+ LK V+ T T RRR + RN + L+ Sbjct: 219 TLEVDTDYAIIFNSKKSLPLKRNVVSPISTHTKKRRRTANTSQRNDGLDLN 269 >SPCC794.03 |||amino acid permease, unknown 13|Schizosaccharomyces pombe|chr 3|||Manual Length = 554 Score = 25.8 bits (54), Expect = 4.9 Identities = 18/55 (32%), Positives = 25/55 (45%) Frame = -1 Query: 204 FSNHVGHTGLITDKSSQMNRFARVVFRERLDLTTMTTGPLFGIESHRPMTGC*KF 40 F N VG G+I D S + +R+VF D +G L ++SH T F Sbjct: 344 FMNVVGILGMIFDTSLSIVASSRLVFAVARDGVLPFSGWLRKVDSHGQPTNAVTF 398 >SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1502 Score = 25.4 bits (53), Expect = 6.5 Identities = 14/31 (45%), Positives = 19/31 (61%) Frame = -1 Query: 459 FASKLILPFLTGFSESLLLAFVPVFVEATST 367 F SKL L + F E + LAF+ F+E TS+ Sbjct: 1032 FFSKLTLQQVQEFYEVVPLAFINCFLEFTSS 1062 >SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1101 Score = 25.0 bits (52), Expect = 8.6 Identities = 17/66 (25%), Positives = 32/66 (48%), Gaps = 1/66 (1%) Frame = +3 Query: 381 LQKLVQMQEEGFH*SQ*EMAG*AWTQINRKRFQEDDPLL*CC-KSHCPHSNEAVKTATKE 557 L KL+Q+ + M+ + I+ K+F+E D L CC +SH ++ ++ Sbjct: 284 LNKLIQLNSAFLDEFEAIMSDLNFEDIDEKKFEEIDLRLACCFESHFFAFSQHYPRYLEQ 343 Query: 558 GSHYGN 575 + +GN Sbjct: 344 SNDFGN 349 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,605,162 Number of Sequences: 5004 Number of extensions: 52709 Number of successful extensions: 124 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 117 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 122 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 267622334 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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