Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10m20r
(696 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 268 5e-73
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 268 5e-73
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 183 2e-47
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 101 1e-22
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 46 4e-06
SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-... 26 4.5
SPCP31B10.05 |||tyrosyl-DNA phosphodiesterase |Schizosaccharomyc... 26 4.5
SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pom... 26 4.5
SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizo... 26 5.9
SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosacchar... 25 7.8
SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomy... 25 7.8
SPAC23H4.16c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 7.8
SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||M... 25 7.8
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 268 bits (657), Expect = 5e-73
Identities = 125/186 (67%), Positives = 141/186 (75%)
Frame = -2
Query: 695 DCSKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIP 516
D +K V YLNEIKDSVVA F WA+KEG M EENLR RFNI DV LH DAIHRGGGQIIP
Sbjct: 648 DQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIP 707
Query: 515 TTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFI 336
T RR +YA L A P + EPV+L EIQ E A+GGIY VLN++RGHVF E Q GTP++
Sbjct: 708 TARRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYN 767
Query: 335 VKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRKG 156
+KAYLPVNESFGFT +LR T GQAFPQ VFDHW + GDP +P SKP +V E RKRKG
Sbjct: 768 IKAYLPVNESFGFTGELRQATAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRKG 827
Query: 155 LKEGLP 138
LKE +P
Sbjct: 828 LKENVP 833
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 268 bits (657), Expect = 5e-73
Identities = 125/186 (67%), Positives = 141/186 (75%)
Frame = -2
Query: 695 DCSKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIP 516
D +K V YLNEIKDSVVA F WA+KEG M EENLR RFNI DV LH DAIHRGGGQIIP
Sbjct: 648 DQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIP 707
Query: 515 TTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFI 336
T RR +YA L A P + EPV+L EIQ E A+GGIY VLN++RGHVF E Q GTP++
Sbjct: 708 TARRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYN 767
Query: 335 VKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRKG 156
+KAYLPVNESFGFT +LR T GQAFPQ VFDHW + GDP +P SKP +V E RKRKG
Sbjct: 768 IKAYLPVNESFGFTGELRQATAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRKG 827
Query: 155 LKEGLP 138
LKE +P
Sbjct: 828 LKENVP 833
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 183 bits (445), Expect = 2e-47
Identities = 84/168 (50%), Positives = 112/168 (66%)
Frame = -2
Query: 695 DCSKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIP 516
D K V LN +K+ + GFQW +EG + +E +R V F + DV L + I+RGGGQIIP
Sbjct: 766 DVDKNV--LNSVKEYIKQGFQWGTREGPLCDETIRNVNFRLMDVVLAPEQIYRGGGQIIP 823
Query: 515 TTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFI 336
T RR Y+ LTA PRLMEPVY+ E+ P ++ IY +L RRRGHV ++ G+P+++
Sbjct: 824 TARRVCYSSFLTASPRLMEPVYMVEVHAPADSLPIIYDLLTRRRGHVLQDIPRPGSPLYL 883
Query: 335 VKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKP 192
V+A +PV +S GF DLR +T GQA Q VFDHWQV+PGDP + KP
Sbjct: 884 VRALIPVIDSCGFETDLRVHTQGQAMCQMVFDHWQVVPGDPLDKSIKP 931
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1000
Score = 101 bits (241), Expect = 1e-22
Identities = 53/161 (32%), Positives = 88/161 (54%), Gaps = 9/161 (5%)
Frame = -2
Query: 668 NEIKDSVVAGFQWAAKEGVMAEENLRGV-----RFNIYDVTLHTDAIHRGG----GQIIP 516
+++ + VV FQ +G + E ++G+ +F+I D + + + GQ+I
Sbjct: 786 SDLSEYVVTAFQLITHQGPLCAEPVQGICVSIDQFDISDDSEDSKLLTINNPQIPGQVIS 845
Query: 515 TTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFI 336
+ + L PRLM +Y C++Q +G +YGV+++RRG V +E GTP FI
Sbjct: 846 VVKESIRHGFLGWSPRLMLAMYSCDVQATSEVLGRVYGVVSKRRGRVIDEEMKEGTPFFI 905
Query: 335 VKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP 213
VKA +PV ESFGF ++ T G A+PQ +F +++L +P
Sbjct: 906 VKALIPVVESFGFAVEILKRTSGAAYPQLIFHGFEMLDENP 946
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 46.4 bits (105), Expect = 4e-06
Identities = 30/125 (24%), Positives = 56/125 (44%)
Frame = -2
Query: 638 FQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLME 459
F A K+G + ++ RF + D H ++ T L A P ++E
Sbjct: 619 FYEALKKGFLIGHPIKNCRFVLEDGAYHPVDSSELAFRL--ATISAFRTAFLQANPMVLE 676
Query: 458 PVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRS 279
P+ I P GG+ G L++R+ + + F ++A +P+N F +++D+R+
Sbjct: 677 PIMNVSITAPVEHQGGVIGNLDKRKATIVDSD--TDEDEFTLQAEVPLNSMFSYSSDIRA 734
Query: 278 NTGGQ 264
T G+
Sbjct: 735 LTKGK 739
>SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+,
L-lysine forming] |Schizosaccharomyces pombe|chr
1|||Manual
Length = 368
Score = 26.2 bits (55), Expect = 4.5
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +1
Query: 511 VVGMIWPPPLWMASVWSVTS*MLNLTPRKFSSAITPSLAA 630
V G+ PPPL + S+ + + + + FS A+ PSL A
Sbjct: 305 VKGVTTPPPLEVISIDHLPTLLPRESSEAFSEALIPSLLA 344
>SPCP31B10.05 |||tyrosyl-DNA phosphodiesterase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 536
Score = 26.2 bits (55), Expect = 4.5
Identities = 30/139 (21%), Positives = 54/139 (38%), Gaps = 4/139 (2%)
Frame = -3
Query: 628 PLRKELWLKRICVVLDSTSMM*HSILMPSIEV-VAKSFQQLEDACTHVC*LLS--PVLWS 458
P K+ W CV L+ H+ + P+++V + + D+ + ++ PV
Sbjct: 50 PQLKQTWQFNFCVDLNFLLENMHASVFPTVDVRITHGYDSKSDSLARLTAQMNHCPVNVK 109
Query: 457 LYIFVKFSVLK*LWXXXXXXXXXXXXTFSKSPRWQVHLCSL*RPTYLSMSRSVL-LPICV 281
LY SV +W S + +H +L P ++ MS+++ P+
Sbjct: 110 LY-----SVYVPMWGTHHSKIMVNFFK-DDSCQIVIHTANLVEPDWIGMSQAIFKTPLLY 163
Query: 280 PTPADRPSRSAYSTIGRSS 224
P D S S+ G S
Sbjct: 164 PKANDSLSTSSVPEYGNPS 182
>SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 166
Score = 26.2 bits (55), Expect = 4.5
Identities = 13/47 (27%), Positives = 23/47 (48%)
Frame = +1
Query: 535 PLWMASVWSVTS*MLNLTPRKFSSAITPSLAAHWNPATTESLISLRY 675
P W+A V ++ S + P FSS + +L A+ N + + + Y
Sbjct: 43 PFWLAEVLAINSFVSIHMPAPFSSVVRNALKANPNSVSIRDITTHYY 89
>SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 391
Score = 25.8 bits (54), Expect = 5.9
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +3
Query: 513 CWNDLATTSMDGISMECYIIDVESNTTQI 599
CW++L+TTS + + II + TT I
Sbjct: 211 CWDELSTTSPESSKVSEPIIQDNTQTTHI 239
>SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 232
Score = 25.4 bits (53), Expect = 7.8
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = -2
Query: 239 HWQVLPGDPCEPQSKPYNVVQETRKRK 159
H+Q L GDP + PY VQ RK
Sbjct: 78 HFQNLSGDPVSQMATPYPPVQIELMRK 104
>SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 25.4 bits (53), Expect = 7.8
Identities = 17/67 (25%), Positives = 29/67 (43%), Gaps = 1/67 (1%)
Frame = -2
Query: 521 IPTTRRCLYACLLTAQPRLMEPVYL-CEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTP 345
IP T RC+ + + + P+L P L + CP + + V+ H F + P
Sbjct: 386 IPNTERCILSAFICSPPQLPLPNPLRMYLPCPSLNSTEV-SVITLAPQHSFLNIVINLNP 444
Query: 344 MFIVKAY 324
+K+Y
Sbjct: 445 ALALKSY 451
>SPAC23H4.16c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 328
Score = 25.4 bits (53), Expect = 7.8
Identities = 23/83 (27%), Positives = 39/83 (46%), Gaps = 7/83 (8%)
Frame = +3
Query: 435 LNFTKIYRLHKTGLSS*QTCVQASSSCWND-----LAT--TSMDGISMECYIIDVESNTT 593
L+++K++ LH +Q S S +ND LA ++ + + Y++ E +
Sbjct: 192 LSYSKVFFLH---------LLQHSDSSFNDKFLLLLANIPVTVSNVEVLLYLLQQEESLA 242
Query: 594 QILFSHNSFLSGPLESSHNRVLN 662
Q + SFL L S HN+V N
Sbjct: 243 QFDLNGKSFLYHMLVSLHNQVTN 265
>SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1317
Score = 25.4 bits (53), Expect = 7.8
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = -2
Query: 674 YLNEIKDSVVAGFQWAAKEGVMAEENLRGVR 582
YL+ + +AGFQ G+ ++N +GVR
Sbjct: 248 YLSPVVRRYLAGFQSGFLHGLKTKDNSKGVR 278
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,030,902
Number of Sequences: 5004
Number of extensions: 65464
Number of successful extensions: 156
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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