Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10m20r
(696 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 276 9e-75
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 186 2e-47
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 186 2e-47
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 183 9e-47
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 105 3e-23
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 54 7e-08
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 54 7e-08
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 50 2e-06
At5g13650.2 68418.m01585 elongation factor family protein contai... 42 5e-04
At5g13650.1 68418.m01584 elongation factor family protein contai... 42 5e-04
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 31 0.96
At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634... 29 2.9
At5g29624.1 68418.m03640 DC1 domain-containing protein contains ... 29 3.9
At4g29220.1 68417.m04180 phosphofructokinase family protein simi... 28 6.8
At2g35490.1 68415.m04347 plastid-lipid associated protein PAP, p... 28 6.8
At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /... 27 9.0
At3g63180.1 68416.m07097 expressed protein 27 9.0
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 276 bits (677), Expect = 9e-75
Identities = 123/185 (66%), Positives = 152/185 (82%)
Frame = -2
Query: 695 DCSKGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIP 516
D KGVQYLNEIKDSVVAGFQWA+KEG +AEEN+RG+ F + DV LH+DAIHRGGGQ+IP
Sbjct: 649 DMCKGVQYLNEIKDSVVAGFQWASKEGPLAEENMRGICFEVCDVVLHSDAIHRGGGQVIP 708
Query: 515 TTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFI 336
T RR +YA +TA+PRL+EPVY+ EIQ PE A+GGIY VLN++RGHVFEE Q GTP++
Sbjct: 709 TARRVIYASQITAKPRLLEPVYMVEIQAPEGALGGIYSVLNQKRGHVFEEMQRPGTPLYN 768
Query: 335 VKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRKG 156
+KAYLPV ESFGF++ LR+ T GQAFPQCVFDHW+++ DP EP ++ +V + RKRKG
Sbjct: 769 IKAYLPVVESFGFSSQLRAATSGQAFPQCVFDHWEMMSSDPLEPGTQASVLVADIRKRKG 828
Query: 155 LKEGL 141
LKE +
Sbjct: 829 LKEAM 833
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 186 bits (452), Expect = 2e-47
Identities = 93/184 (50%), Positives = 118/184 (64%), Gaps = 12/184 (6%)
Frame = -2
Query: 662 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLL 483
+KDS+V GFQW A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L
Sbjct: 776 VKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFL 835
Query: 482 TAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESF 303
A PRLMEPVY EIQ P V IY VL+RRRGHV + GTP +IVKA+LPV ESF
Sbjct: 836 MATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESF 895
Query: 302 GFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEP--QSKPY----------NVVQETRKRK 159
GF DLR +T GQAF VFDHW ++PGDP + Q +P + +TR+RK
Sbjct: 896 GFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRK 955
Query: 158 GLKE 147
G+ E
Sbjct: 956 GMSE 959
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 186 bits (452), Expect = 2e-47
Identities = 93/184 (50%), Positives = 118/184 (64%), Gaps = 12/184 (6%)
Frame = -2
Query: 662 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLL 483
+KDS+V GFQW A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L
Sbjct: 776 VKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFL 835
Query: 482 TAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESF 303
A PRLMEPVY EIQ P V IY VL+RRRGHV + GTP +IVKA+LPV ESF
Sbjct: 836 MATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESF 895
Query: 302 GFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEP--QSKPY----------NVVQETRKRK 159
GF DLR +T GQAF VFDHW ++PGDP + Q +P + +TR+RK
Sbjct: 896 GFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRK 955
Query: 158 GLKE 147
G+ E
Sbjct: 956 GMSE 959
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 183 bits (446), Expect = 9e-47
Identities = 92/184 (50%), Positives = 118/184 (64%), Gaps = 12/184 (6%)
Frame = -2
Query: 662 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLL 483
+KDS+V GFQW A+EG + +E +R V+F I D + + +HRG GQ+IPT RR Y+ L
Sbjct: 762 VKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFL 821
Query: 482 TAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESF 303
A PRLMEPVY EIQ P V IY VL+RRRG+V + GTP +IVKA+LPV ESF
Sbjct: 822 MATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESF 881
Query: 302 GFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEP--QSKPY----------NVVQETRKRK 159
GF DLR +T GQAF VFDHW ++PGDP + Q +P + +TR+RK
Sbjct: 882 GFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRK 941
Query: 158 GLKE 147
G+ E
Sbjct: 942 GMSE 945
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 105 bits (252), Expect = 3e-23
Identities = 55/158 (34%), Positives = 84/158 (53%), Gaps = 8/158 (5%)
Frame = -2
Query: 662 IKDSVVAGFQWAAKEGVMAEENLRGVRFNIYD-----VTLHTDAIHRGG---GQIIPTTR 507
++ S+V+GFQ A G + +E + G+ F I + TD G GQ++ +
Sbjct: 801 LESSIVSGFQLATASGPLCDEPMWGLAFTIESHLAPAEDVETDKPENFGIFTGQVMTAVK 860
Query: 506 RCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKA 327
A +L PR++E +Y CE+ +G +Y VL+RRR + +E G+ +F V A
Sbjct: 861 DACRAAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEGSSLFTVHA 920
Query: 326 YLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP 213
Y+PV+ESFGF +LR T G A V HW++L DP
Sbjct: 921 YVPVSESFGFADELRKGTSGGASALMVLSHWEMLEEDP 958
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 54.4 bits (125), Expect = 7e-08
Identities = 36/133 (27%), Positives = 62/133 (46%), Gaps = 3/133 (2%)
Frame = -2
Query: 653 SVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYA---CLL 483
++ GF+ AA G + + +R + L A H + +YA C
Sbjct: 597 AIEKGFKEAANSGSLIGHPVENLR-----IVLTDGASHAVDSSELAFKMAAIYAFRLCYT 651
Query: 482 TAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESF 303
A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N F
Sbjct: 652 AARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMF 709
Query: 302 GFTADLRSNTGGQ 264
G++ LRS T G+
Sbjct: 710 GYSTSLRSMTQGK 722
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 54.4 bits (125), Expect = 7e-08
Identities = 36/133 (27%), Positives = 62/133 (46%), Gaps = 3/133 (2%)
Frame = -2
Query: 653 SVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYA---CLL 483
++ GF+ AA G + + +R + L A H + +YA C
Sbjct: 597 AIEKGFKEAANSGSLIGHPVENLR-----IVLTDGASHAVDSSELAFKMAAIYAFRLCYT 651
Query: 482 TAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESF 303
A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N F
Sbjct: 652 AARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMF 709
Query: 302 GFTADLRSNTGGQ 264
G++ LRS T G+
Sbjct: 710 GYSTSLRSMTQGK 722
>At1g62750.1 68414.m07082 elongation factor Tu family protein similar
to elongation factor G SP:P34811 [Glycine max (Soybean)]
Length = 783
Score = 49.6 bits (113), Expect = 2e-06
Identities = 41/155 (26%), Positives = 66/155 (42%)
Frame = -2
Query: 686 KGVQYLNEIKDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTR 507
KG E V+ G + GV+A + VR + D + H Q+ R
Sbjct: 616 KGGAVPREYIPGVMKGLEECMSTGVLAGFPVVDVRACLVDGSYHDVDSSVLAFQL--AAR 673
Query: 506 RCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKA 327
+ A PR++EP+ E+ PE +G + G LN RRG + G + +V +
Sbjct: 674 GAFREGMRKAGPRMLEPIMRVEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDS 732
Query: 326 YLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLP 222
+P+ E F + + LR T G+A + V+P
Sbjct: 733 LVPLAEMFQYVSTLRGMTKGRASYTMQLAKFDVVP 767
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 41.5 bits (93), Expect = 5e-04
Identities = 21/85 (24%), Positives = 39/85 (45%)
Frame = -2
Query: 470 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 291
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 473 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 532
Query: 290 DLRSNTGGQAFPQCVFDHWQVLPGD 216
+ + + G A VFD + GD
Sbjct: 533 AILTASRGTAILNTVFDSYGPWAGD 557
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 41.5 bits (93), Expect = 5e-04
Identities = 21/85 (24%), Positives = 39/85 (45%)
Frame = -2
Query: 470 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 291
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 472 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 531
Query: 290 DLRSNTGGQAFPQCVFDHWQVLPGD 216
+ + + G A VFD + GD
Sbjct: 532 AILTASRGTAILNTVFDSYGPWAGD 556
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 30.7 bits (66), Expect = 0.96
Identities = 21/68 (30%), Positives = 32/68 (47%), Gaps = 1/68 (1%)
Frame = -2
Query: 461 EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNE-SFGFTADL 285
EP + I P VG + + + RRG E + + +F+ K LP+ E F +L
Sbjct: 466 EPTVIATIILPSEYVGAVINLCSDRRGQQLEYTFIDAQRVFL-KYQLPLREIVVDFYDEL 524
Query: 284 RSNTGGQA 261
+S T G A
Sbjct: 525 KSITSGYA 532
>At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634:
Arabidopsis thaliana protein of unknown function
(DUF794)
Length = 340
Score = 29.1 bits (62), Expect = 2.9
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = +2
Query: 176 PVQRCRACSEVRTGLQG 226
PV RCR C+EV G QG
Sbjct: 62 PVHRCRLCAEVHIGKQG 78
>At5g29624.1 68418.m03640 DC1 domain-containing protein contains
Pfam PF03107: DC1 domain
Length = 488
Score = 28.7 bits (61), Expect = 3.9
Identities = 19/80 (23%), Positives = 27/80 (33%)
Frame = +3
Query: 345 RCTCHLGLFENXXXXXXXXXXXXXHSYFRTLNFTKIYRLHKTGLSS*QTCVQASSSCWND 524
RC C + H L K + LHK L+ TC+ + C
Sbjct: 309 RCQCCILAINGDPCYRCVECDFILHDACANLPRKKQHLLHKHKLTLSSTCIDGETYCQCK 368
Query: 525 LATTSMDGISMECYIIDVES 584
+ DG +C+ D ES
Sbjct: 369 VYDVYTDGFIYKCHHEDCES 388
>At4g29220.1 68417.m04180 phosphofructokinase family protein similar
to phosphofructokinase [Amycolatopsis methanolica]
GI:17432243; contains Pfam profile PF00365:
Phosphofructokinase
Length = 473
Score = 27.9 bits (59), Expect = 6.8
Identities = 34/131 (25%), Positives = 55/131 (41%), Gaps = 6/131 (4%)
Frame = -2
Query: 671 LNEIKDSVVAG--FQWAAKEGVMAEENLRGVRF-NIYDVTLHT-DAIHRGGGQIIPTTR- 507
LN + +V G + + K + + RG N + L T + IHR GG I+ T+R
Sbjct: 107 LNTVIREIVCGLSYMYGVKRILGIDGGYRGFYARNTIHLDLKTVNDIHRSGGTILGTSRG 166
Query: 506 -RCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVK 330
+ + Q R + VY+ + I+ + +R+ V VAG P I
Sbjct: 167 GHNTTKIVDSIQDRGINQVYIIGGDGSQKGAAAIFEEIRKRKLKV----AVAGIPKTIDN 222
Query: 329 AYLPVNESFGF 297
++ SFGF
Sbjct: 223 DIPIIDRSFGF 233
>At2g35490.1 68415.m04347 plastid-lipid associated protein PAP,
putative similar to plastid-lipid associated protein
PAP3 [Brassica rapa] GI:14248552; contains Pfam profile
PF04755: PAP_fibrillin
Length = 376
Score = 27.9 bits (59), Expect = 6.8
Identities = 12/22 (54%), Positives = 13/22 (59%)
Frame = -2
Query: 248 VFDHWQVLPGDPCEPQSKPYNV 183
V D W PGD EP S+P NV
Sbjct: 71 VTDEWGEKPGDANEPDSQPDNV 92
>At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase PG1 [Glycine max]
GI:5669846; contains PF00295: Glycosyl hydrolases family
28
Length = 444
Score = 27.5 bits (58), Expect = 9.0
Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
Frame = -1
Query: 387 SWSRFRRVPGGRY-TYVHCEGL 325
S+ R R +PGGRY +++H +GL
Sbjct: 123 SYGRGRELPGGRYMSFIHGDGL 144
>At3g63180.1 68416.m07097 expressed protein
Length = 978
Score = 27.5 bits (58), Expect = 9.0
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = -3
Query: 328 PTYLSMSRSVLLPICVPTPADRPSRSAYSTIGRSS 224
P ++ + L P+ VP+ + RP++S + +G +S
Sbjct: 539 PAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLAS 573
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,058,456
Number of Sequences: 28952
Number of extensions: 356720
Number of successful extensions: 938
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 904
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 932
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1487069504
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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