BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10l23r (672 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein L5|Sch... 186 3e-48 SPAC3H5.12c |rpl501|rpl5-1, rpl5|60S ribosomal protein L5|Schizo... 184 8e-48 SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces pomb... 29 0.46 SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces pomb... 29 0.81 SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 28 1.1 SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 27 1.9 SPBC25B2.10 |||Usp |Schizosaccharomyces pombe|chr 2|||Manual 27 2.5 SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces po... 27 3.3 SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces po... 26 4.3 SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 26 4.3 SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual 26 4.3 SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 26 5.7 SPAC22F3.08c |rok1||ATP-dependent RNA helicase Rok1 |Schizosacch... 26 5.7 SPAC140.04 |||conserved fungal protein|Schizosaccharomyces pombe... 26 5.7 SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual 25 7.5 SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr 1|||... 25 7.5 SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 25 7.5 SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces p... 25 9.9 SPBC713.07c |||vacuolar polyphosphatase |Schizosaccharomyces pom... 25 9.9 SPAC18G6.02c |chp1||chromodomain protein Chp1|Schizosaccharomyce... 25 9.9 >SPBC11C11.09c |rpl502|rpl5-2, rpl5b|60S ribosomal protein L5|Schizosaccharomyces pombe|chr 2|||Manual Length = 294 Score = 186 bits (453), Expect = 3e-48 Identities = 94/217 (43%), Positives = 132/217 (60%), Gaps = 2/217 (0%) Frame = -3 Query: 670 GDHIVCAAYSHELPRYGVKVGLTNYAAAYSTGXXXXXXXXXXXXXXXXXXXXXXXXXDEY 491 GD+++ A+S ELPRYG+K GL N+ AAY+TG + Sbjct: 71 GDYVLAHAHSSELPRYGIKWGLANWTAAYATGLLVARRALAKVGLADKYEGVTEPEGEFE 130 Query: 490 NVEPVDNGPGAFRCYLDVGLARTTTGARVFGAMKGAVDGGLNVPHSIKRFPGYDAESKKF 311 E +++GP F+ +LDVGL RT+TG+RVFGAMKGA DGGL +PHS RFPG+D E+++ Sbjct: 131 LTEAIEDGPRPFKVFLDVGLKRTSTGSRVFGAMKGASDGGLFIPHSPNRFPGFDIETEEL 190 Query: 310 NAEVHRAHIFGLHVAEYMRSLEQDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRAD 131 + E R +I+G HVAEYM L DDE+ +++QFS I G+ +D +E IY +A+ IR D Sbjct: 191 DDETLRKYIYGGHVAEYMEMLIDDDEERYQKQFSGLIADGIESDQLEDIYAEAYAKIRED 250 Query: 130 PSHKK--KELKKDSVKQKRWNKRKLTLAERKNRIKQK 26 PS +K K+ + ++ +RKLT ERK R K Sbjct: 251 PSFQKSGKDAAAFKAESLKYTQRKLTAEERKERFNAK 287 >SPAC3H5.12c |rpl501|rpl5-1, rpl5|60S ribosomal protein L5|Schizosaccharomyces pombe|chr 1|||Manual Length = 294 Score = 184 bits (449), Expect = 8e-48 Identities = 94/217 (43%), Positives = 131/217 (60%), Gaps = 2/217 (0%) Frame = -3 Query: 670 GDHIVCAAYSHELPRYGVKVGLTNYAAAYSTGXXXXXXXXXXXXXXXXXXXXXXXXXDEY 491 GD+++ A+S ELPRYG+K GL N+ AAY+TG + Sbjct: 71 GDYVLAHAHSSELPRYGIKWGLANWTAAYATGLLVARRALAKVGLADKYEGVTEPEGEFE 130 Query: 490 NVEPVDNGPGAFRCYLDVGLARTTTGARVFGAMKGAVDGGLNVPHSIKRFPGYDAESKKF 311 E +++GP F+ +LDVGL RT+TG+RVFGAMKGA DGGL +PHS RFPG+D E+++ Sbjct: 131 LTEAIEDGPRPFKVFLDVGLKRTSTGSRVFGAMKGASDGGLFIPHSPNRFPGFDIETEEL 190 Query: 310 NAEVHRAHIFGLHVAEYMRSLEQDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRAD 131 + E R +I+G HVAEYM L DDE+ +++QFS I G+ +D +E IY +A+ IR D Sbjct: 191 DDETLRKYIYGGHVAEYMEMLIDDDEERYQKQFSGLIADGIESDQLEDIYAEAYAKIRED 250 Query: 130 PSHKK--KELKKDSVKQKRWNKRKLTLAERKNRIKQK 26 PS +K K+ + + +RKLT ERK R K Sbjct: 251 PSFQKSGKDAAAFKAESLKHTQRKLTAEERKERFNAK 287 >SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces pombe|chr 1|||Manual Length = 508 Score = 29.5 bits (63), Expect = 0.46 Identities = 19/53 (35%), Positives = 27/53 (50%) Frame = -3 Query: 175 IEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKAS 17 IE KK+ + AD KKE K+ K+ + +KRK +E K+KK S Sbjct: 451 IEKKKKKSSKLKEADGESSKKEKKEKKDKKHKKSKRKSEESEDGESPKKKKKS 503 >SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces pombe|chr 2|||Manual Length = 497 Score = 28.7 bits (61), Expect = 0.81 Identities = 17/45 (37%), Positives = 24/45 (53%), Gaps = 1/45 (2%) Frame = -3 Query: 148 EAIRADPSHKKKELKKDSVKQKRWNKRKL-TLAERKNRIKQKKAS 17 E I PS K+K+ KK+ K+K KR +E + K+KK S Sbjct: 452 ETISEKPSKKEKKDKKEKKKEKSKKKRSADDASEEVKKSKKKKKS 496 >SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1184 Score = 28.3 bits (60), Expect = 1.1 Identities = 17/55 (30%), Positives = 33/55 (60%), Gaps = 2/55 (3%) Frame = -3 Query: 160 KKAHEAIRADPSHKKKELKKDSVKQKRWNKR--KLTLAERKNRIKQKKASFIKRL 2 + A E R + + + K LKK + ++KR K+ K AE K R+++++A + K++ Sbjct: 708 RAAQERERKEQAKEAKRLKKLAKEEKRLKKKEEKARKAEEK-RLQKERAKYAKQM 761 >SPCC162.08c |nup211||nuclear pore complex associated protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1837 Score = 27.5 bits (58), Expect = 1.9 Identities = 13/54 (24%), Positives = 29/54 (53%) Frame = -3 Query: 244 QDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRADPSHKKKELKKDSVKQK 83 +++++S ++ +K ++ V +KK +E IR+D LK+D K++ Sbjct: 1023 KEEKESSSKELAKQLEDAVREKDSALSFKKDYEKIRSDADRVITSLKEDIEKER 1076 >SPBC25B2.10 |||Usp |Schizosaccharomyces pombe|chr 2|||Manual Length = 307 Score = 27.1 bits (57), Expect = 2.5 Identities = 11/31 (35%), Positives = 20/31 (64%) Frame = +1 Query: 550 EAVFVLIADQLNMLQHNLSDQPSHHNVATHV 642 EAV + + D + L +LSD+ S+ ++A H+ Sbjct: 169 EAVVLRVIDPSSKLAEDLSDEQSYRSLAEHI 199 >SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 26.6 bits (56), Expect = 3.3 Identities = 13/38 (34%), Positives = 22/38 (57%) Frame = -3 Query: 178 AIEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWNKRK 65 ++E+ K EA + + +KKE KK+ K+K+ K K Sbjct: 430 SVESSEKDEDEAAKKEEKRRKKEAKKEK-KEKKEKKEK 466 >SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 284 Score = 26.2 bits (55), Expect = 4.3 Identities = 22/79 (27%), Positives = 36/79 (45%), Gaps = 2/79 (2%) Frame = -3 Query: 247 EQDDEDSFKRQFSKYIKLGVTADA--IEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWN 74 E+ + S K++ K G D +E I KK + HKKK+ + S K+K + Sbjct: 166 EKRKKHSSKKKSKKKTSTGSALDPKKLEKITKKKKK------EHKKKDKESSSKKRKSGS 219 Query: 73 KRKLTLAERKNRIKQKKAS 17 K ++K ++K K S Sbjct: 220 SDKEEKKKKKIKLKDKPES 238 >SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizosaccharomyces pombe|chr 2|||Manual Length = 932 Score = 26.2 bits (55), Expect = 4.3 Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 1/57 (1%) Frame = -3 Query: 172 EAIYKKAHEAIRADPSHKKKELKKDSVKQKR-WNKRKLTLAERKNRIKQKKASFIKR 5 EAI + EA RA +EL + QKR +R E K R +++ A ++R Sbjct: 788 EAIREAEEEAARAAEEEANRELHEQEEAQKRAIEERTRAAREAKEREQREMAEKLER 844 >SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 26.2 bits (55), Expect = 4.3 Identities = 15/28 (53%), Positives = 19/28 (67%) Frame = -3 Query: 187 TADAIEAIYKKAHEAIRADPSHKKKELK 104 TA IEA+YKKA+ I+ D +H K LK Sbjct: 174 TAQLIEAVYKKAN--IK-DAAHDSKNLK 198 >SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1049 Score = 25.8 bits (54), Expect = 5.7 Identities = 23/101 (22%), Positives = 39/101 (38%) Frame = -3 Query: 313 FNAEVHRAHIFGLHVAEYMRSLEQDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRA 134 FNA R+ + A+ D E F + G A+ +IY+ + E IR+ Sbjct: 363 FNASGERSESANVETAQVW----DDREQYFFYEVFPNFNEGSIAEMKSSIYESSQEGIRS 418 Query: 133 DPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKASFI 11 + KKE D +K + ++ R + K S + Sbjct: 419 SSENNKKE---DDLKDSTGDLNTTQVSSRVDNFLLKLPSMV 456 >SPAC22F3.08c |rok1||ATP-dependent RNA helicase Rok1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 481 Score = 25.8 bits (54), Expect = 5.7 Identities = 21/75 (28%), Positives = 35/75 (46%) Frame = -3 Query: 226 FKRQFSKYIKLGVTADAIEAIYKKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAER 47 F ++ +YIKL A + + + + A P KE+KK K KR T A Sbjct: 392 FTKEDGEYIKL--IAGVMRSSGCEVPNWVMALPK-PSKEMKKKLKKSPPKRKRITTRASY 448 Query: 46 KNRIKQKKASFIKRL 2 + +Q+K +IK++ Sbjct: 449 DRQKEQRKKEYIKKV 463 >SPAC140.04 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 295 Score = 25.8 bits (54), Expect = 5.7 Identities = 14/52 (26%), Positives = 26/52 (50%) Frame = -3 Query: 160 KKAHEAIRADPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQKKASFIKR 5 KK +E + SH+K K KRKL + +++ K+ +++ IK+ Sbjct: 27 KKRYEDEKRKISHQKYTDKLLEANDNETTKRKLLVKQKRKSNKEFQSNIIKK 78 >SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual Length = 978 Score = 25.4 bits (53), Expect = 7.5 Identities = 10/24 (41%), Positives = 17/24 (70%) Frame = -3 Query: 268 AEYMRSLEQDDEDSFKRQFSKYIK 197 +EY+++L D SFK+ F K+I+ Sbjct: 948 SEYLQALSNLDSRSFKQFFQKFIQ 971 >SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr 1|||Manual Length = 948 Score = 25.4 bits (53), Expect = 7.5 Identities = 10/21 (47%), Positives = 14/21 (66%) Frame = -1 Query: 174 LKPSTRKPMKPSVRIHPTRRK 112 L PS RKP+ V++HP +K Sbjct: 384 LLPSLRKPLLNFVKVHPPSQK 404 >SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 207 Score = 25.4 bits (53), Expect = 7.5 Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 2/49 (4%) Frame = -3 Query: 160 KKAHEAIRADPSHKKKE--LKKDSVKQKRWNKRKLTLAERKNRIKQKKA 20 KK E A S K+ E KKD+ + ++W+K T + + K+K A Sbjct: 5 KKRAEKAEAAKSRKQDEEKKKKDAEEDEKWSKGVKTNKKEQEAEKRKAA 53 >SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 480 Score = 25.0 bits (52), Expect = 9.9 Identities = 12/39 (30%), Positives = 22/39 (56%) Frame = -2 Query: 122 QEERVKERLCQTEALEQTQANIGREEKQNQAKEGFLHQE 6 +EER K+ L + E E+ + + E+K + ++ L QE Sbjct: 133 EEERRKKELMEKEKKEKERIRLIEEQKHKENEQRRLKQE 171 >SPBC713.07c |||vacuolar polyphosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 577 Score = 25.0 bits (52), Expect = 9.9 Identities = 11/19 (57%), Positives = 15/19 (78%) Frame = -3 Query: 121 KKKELKKDSVKQKRWNKRK 65 KKK+ KK + K+K+ NKRK Sbjct: 436 KKKKKKKKNNKKKKKNKRK 454 >SPAC18G6.02c |chp1||chromodomain protein Chp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 960 Score = 25.0 bits (52), Expect = 9.9 Identities = 12/36 (33%), Positives = 22/36 (61%) Frame = -3 Query: 133 DPSHKKKELKKDSVKQKRWNKRKLTLAERKNRIKQK 26 D KK + +K+ ++Q+R KRK L + ++K+K Sbjct: 89 DNEAKKMKREKEILRQQR-QKRKSELTQLSQKVKEK 123 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,590,497 Number of Sequences: 5004 Number of extensions: 49805 Number of successful extensions: 244 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 221 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 238 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 307866294 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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