BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10l21r (705 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 55 9e-09 SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces ... 55 9e-09 SPAC22A12.01c |pso2|snm1, SPAC56F8.17c, snm1|DNA 5' exonuclease ... 30 0.28 SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|c... 29 0.86 SPBC26H8.09c |snf59||SWI/SNF complex subunit Snf59|Schizosacchar... 27 2.0 SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog... 27 2.0 SPBC725.13c |psf2|dre13, bsh3|GINS complex subunit Psf2|Schizosa... 27 2.6 SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 27 2.6 SPBC15D4.05 |||conserved protein|Schizosaccharomyces pombe|chr 2... 26 4.6 SPBC947.01 |||AAA family ATPase, unknown biological role|Schizos... 26 4.6 SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6 |Schizosacc... 26 6.0 SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 26 6.0 SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor Cwf22|Schizosacch... 26 6.0 SPCC1322.05c |||leukotriene A-4 hydrolase |Schizosaccharomyces p... 25 8.0 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 25 8.0 SPBC336.12c |cdc10||MBF transcription factor complex subunit Cdc... 25 8.0 >SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 541 Score = 55.2 bits (127), Expect = 9e-09 Identities = 51/184 (27%), Positives = 79/184 (42%), Gaps = 14/184 (7%) Frame = -1 Query: 678 NKSKSDDEKPVLEKLLDINEEYAYIMASDMLVAGVDTTSNTMSATLYLMAINQDKQQKLR 499 +++K EKP + + + ++E + L A D TS+ M+ L+A + D QK+R Sbjct: 288 SENKEGAEKPSV-LIREFSDEEISLTFLSFLFASQDATSSAMTWLFQLLADHPDVLQKVR 346 Query: 498 EEVMS-KNG------------KRSYLRACIKEAMRILPVVSGNMRRTTKEYNIL-GYHIP 361 EE + + G K +Y RA +KE +R+ P V R K + I Y +P Sbjct: 347 EEQLRIRKGDIDVPLSLDLMEKMTYTRAVVKECLRLRPPVLMVPYRVKKAFPITPDYTVP 406 Query: 360 ENVDIAFAHQHLSMMEKYYPRPTEFIPERWLTNKSDPLYYGNAHPFANSPFGFGVRSCIG 181 ++ + K YP P F P+RW N P FG G C+G Sbjct: 407 KDAMVIPTLYGALHDSKVYPEPETFNPDRWAPNG-----LAEQSPKNWMVFGNGPHVCLG 461 Query: 180 RRIA 169 +R A Sbjct: 462 QRYA 465 >SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces pombe|chr 1|||Manual Length = 495 Score = 55.2 bits (127), Expect = 9e-09 Identities = 51/197 (25%), Positives = 91/197 (46%), Gaps = 25/197 (12%) Frame = -1 Query: 591 MLVAGVDTTSNTMSATLYLMAINQDKQQKLREE---VMSKNGKRSY--------LRACIK 445 +L+AG T++ T+ L L+ + + L EE V+ +N + + L I+ Sbjct: 285 LLMAGQHTSAATIVWVLALLGSKPEIIEMLWEEQKRVVGENLELKFDQYKDMPLLNYVIQ 344 Query: 444 EAMRILPVVSGNMRRTTKEYNILGYHI--PENVDIAFAHQHLSMMEKYYPRPTEFIPERW 271 E +R+ P + +MR+ ++ + G I P N + A + E+Y+ T+F P+RW Sbjct: 345 ETLRLHPPIHSHMRKVKRDLPVPGSKIVIPANNYLLAAPGLTATEEEYFTHATDFDPKRW 404 Query: 270 -----LTNKSDPLYY-------GNAHPFANSPFGFGVRSCIGRRIAELEVETFLSKIVEN 127 ++ + Y G A P+ PFG G CIG + A + + T +SK V + Sbjct: 405 NDRVNEDENAEQIDYGYGLVTKGAASPYL--PFGAGRHRCIGEQFAYMHLSTIISKFVHD 462 Query: 126 FQVEWSGSSPRVEQTSI 76 + G P V+ +S+ Sbjct: 463 YTWTLIGKVPNVDYSSM 479 >SPAC22A12.01c |pso2|snm1, SPAC56F8.17c, snm1|DNA 5' exonuclease |Schizosaccharomyces pombe|chr 1|||Manual Length = 560 Score = 30.3 bits (65), Expect = 0.28 Identities = 17/48 (35%), Positives = 27/48 (56%) Frame = -1 Query: 447 KEAMRILPVVSGNMRRTTKEYNILGYHIPENVDIAFAHQHLSMMEKYY 304 K+A+ LP N+R Y ++ Y+IP VD AFA+ + +E Y+ Sbjct: 166 KQALLPLPGKQINVRSVVPFYKLMPYNIPFAVD-AFAYGAIDGVEAYF 212 >SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|chr 1|||Manual Length = 1315 Score = 28.7 bits (61), Expect = 0.86 Identities = 19/69 (27%), Positives = 39/69 (56%), Gaps = 5/69 (7%) Frame = -1 Query: 696 LDDIKMNKSKSDDEKPVLEKLLDINEEYAYIMASDMLVAG--VDTTSNT-MSATLYLMAI 526 L ++ + S+S++ P+ E+++DI E YA+ + + G +DT +S L + + Sbjct: 1168 LKNLTLRFSQSENVFPI-EQIIDITERYAFDQQGEAVATGWVIDTFLGAGVSHELIFIVL 1226 Query: 525 NQ--DKQQK 505 NQ D+++K Sbjct: 1227 NQLYDRREK 1235 >SPBC26H8.09c |snf59||SWI/SNF complex subunit Snf59|Schizosaccharomyces pombe|chr 2|||Manual Length = 515 Score = 27.5 bits (58), Expect = 2.0 Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 2/46 (4%) Frame = +2 Query: 95 LGDDPDH--ST*KFSTIFDKNVSTSNSAMRRPIQLRTPKPNGEFAN 226 + ++P H S+ K ST D N + S ++ ++ PKP+G+F N Sbjct: 114 MSEEPKHHDSSNKESTNLD-NSNMDESENQKNFKIEEPKPSGDFRN 158 >SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 982 Score = 27.5 bits (58), Expect = 2.0 Identities = 21/110 (19%), Positives = 48/110 (43%), Gaps = 4/110 (3%) Frame = -1 Query: 687 IKMNKSKSDDEKPVLEKLLDINEEYAYI--MASDMLVAGVDTTSNTMSATLYLMAINQDK 514 I+ ++ + D + L K+LD +A + + + +A + + L + D+ Sbjct: 237 IRSSEFSARDVELELSKVLDSPVTHALVPELGLQLAMASCNALLRYLGPALLNPDMEDDR 296 Query: 513 QQKLREEVMSKNGKRSYLRACIK--EAMRILPVVSGNMRRTTKEYNILGY 370 + R+ + + Y+R I ++ +LP +GN +T Y +L + Sbjct: 297 ENTSRKLHLYHHNLEQYMRLDIAAVRSLNLLPPPNGNAHKTMSLYGLLNH 346 >SPBC725.13c |psf2|dre13, bsh3|GINS complex subunit Psf2|Schizosaccharomyces pombe|chr 2|||Manual Length = 183 Score = 27.1 bits (57), Expect = 2.6 Identities = 13/43 (30%), Positives = 22/43 (51%) Frame = -2 Query: 404 DAPQRSTTFLDIIYRKMSTLHLLINICR*WRNTIQDRRSSFLN 276 D + TF ++ +R + T HLL+N C ++D R L+ Sbjct: 86 DDELENETFSELPFRWLETAHLLLNFCADDIEDVEDIRRILLD 128 >SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 3655 Score = 27.1 bits (57), Expect = 2.6 Identities = 30/132 (22%), Positives = 62/132 (46%), Gaps = 3/132 (2%) Frame = -1 Query: 639 KLLDINEEYAYIMASDMLVAGVDTTSN--TMSATLYLMAINQDKQQKLREEVMSKNGKRS 466 +L EE AY + +L G+ S +S + + ++Q Q+ E V+ + + + Sbjct: 3146 RLKSFPEEDAYRLIVALLNDGLQYISRLGVVSKNTFQLPMSQANIQRFAENVLPVSVREA 3205 Query: 465 YLRACIKEAMRILPVVSGNMRRTTKEY-NILGYHIPENVDIAFAHQHLSMMEKYYPRPTE 289 +LR ++ + +L V +R K++ NIL P+ + + +LS + E Sbjct: 3206 FLRDFVETKLDLLTYVD-KLRMWRKKFENILDQR-PKFLHLEQCSLYLSEFQHQKFDEVE 3263 Query: 288 FIPERWLTNKSD 253 IP ++L +K++ Sbjct: 3264 -IPGQYLLDKNN 3274 >SPBC15D4.05 |||conserved protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 411 Score = 26.2 bits (55), Expect = 4.6 Identities = 14/52 (26%), Positives = 26/52 (50%) Frame = -1 Query: 624 NEEYAYIMASDMLVAGVDTTSNTMSATLYLMAINQDKQQKLREEVMSKNGKR 469 N+ ++ SD L+ D+T + + T+ + K LR + ++NGKR Sbjct: 38 NDIDSFEQQSDFLLVDHDSTLDPVPVTILTGFLGAGKTSLLRSILENRNGKR 89 >SPBC947.01 |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 660 Score = 26.2 bits (55), Expect = 4.6 Identities = 11/30 (36%), Positives = 16/30 (53%) Frame = +2 Query: 134 TIFDKNVSTSNSAMRRPIQLRTPKPNGEFA 223 TI K +S S + P+Q TP + E+A Sbjct: 335 TIVSKTISASTTQQTEPLQQTTPSSDFEYA 364 >SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6 |Schizosaccharomyces pombe|chr 1|||Manual Length = 745 Score = 25.8 bits (54), Expect = 6.0 Identities = 11/27 (40%), Positives = 17/27 (62%) Frame = +2 Query: 134 TIFDKNVSTSNSAMRRPIQLRTPKPNG 214 T++DKN T ++A+R +R P NG Sbjct: 114 TLYDKNPLTRSTAIRVMSSIRVPAING 140 >SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1628 Score = 25.8 bits (54), Expect = 6.0 Identities = 10/28 (35%), Positives = 15/28 (53%) Frame = -1 Query: 291 EFIPERWLTNKSDPLYYGNAHPFANSPF 208 EF+PE WL ++ D + N + N F Sbjct: 122 EFLPEEWLLSRLDCKFLENINVVPNGDF 149 >SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor Cwf22|Schizosaccharomyces pombe|chr 2|||Manual Length = 834 Score = 25.8 bits (54), Expect = 6.0 Identities = 12/41 (29%), Positives = 19/41 (46%) Frame = +3 Query: 273 SVQE*TPSVLDSISPSSTNVDEQMQCRHFPVYDIQECCTPL 395 SV + P V+D + S N+ + + D +ECC L Sbjct: 393 SVNDEKPQVIDQTNASLVNLRKSIYLTIMSSVDFEECCHKL 433 >SPCC1322.05c |||leukotriene A-4 hydrolase |Schizosaccharomyces pombe|chr 3|||Manual Length = 612 Score = 25.4 bits (53), Expect = 8.0 Identities = 7/13 (53%), Positives = 11/13 (84%) Frame = -3 Query: 577 CRYHFKHDVRNFV 539 C+Y F+HD+ NF+ Sbjct: 228 CKYEFEHDMENFM 240 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 25.4 bits (53), Expect = 8.0 Identities = 11/45 (24%), Positives = 23/45 (51%) Frame = -1 Query: 174 IAELEVETFLSKIVENFQVEWSGSSPRVEQTSINYFKGPFNFIFK 40 + EL +E ++ ++ ++V+ PRV K PF+++ K Sbjct: 520 MGELHLEVYVERMRREYKVDCETGKPRVAFRETLSKKVPFSYLHK 564 >SPBC336.12c |cdc10||MBF transcription factor complex subunit Cdc10|Schizosaccharomyces pombe|chr 2|||Manual Length = 767 Score = 25.4 bits (53), Expect = 8.0 Identities = 13/29 (44%), Positives = 17/29 (58%) Frame = -3 Query: 595 RHAGGWCRYHFKHDVRNFVPHGDKSGQTA 509 +HA G Y K D N++ H DK+G TA Sbjct: 460 KHASGNNGYMLK-DFINYLNHQDKNGDTA 487 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,028,480 Number of Sequences: 5004 Number of extensions: 67950 Number of successful extensions: 247 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 233 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 246 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 327172622 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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