Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10l18r
(735 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g04410.1 68414.m00432 malate dehydrogenase, cytosolic, putati... 167 9e-42
At5g43330.1 68418.m05296 malate dehydrogenase, cytosolic, putati... 165 4e-41
At5g56720.1 68418.m07079 malate dehydrogenase, cytosolic, putati... 162 3e-40
At5g58330.3 68418.m07302 malate dehydrogenase [NADP], chloroplas... 78 7e-15
At5g58330.2 68418.m07304 malate dehydrogenase [NADP], chloroplas... 78 7e-15
At5g58330.1 68418.m07303 malate dehydrogenase [NADP], chloroplas... 78 7e-15
At4g15920.1 68417.m02418 nodulin MtN3 family protein similar to ... 28 7.4
At3g26855.1 68416.m03360 hypothetical protein 28 7.4
At3g14840.2 68416.m01875 leucine-rich repeat family protein / pr... 28 7.4
>At1g04410.1 68414.m00432 malate dehydrogenase, cytosolic, putative
strong similarity to malate dehydrogenase from
Mesembryanthemum crystallinum [SP|O24047], Medicago
sativa [SP|O48905], Prunus persica [GI:15982948];
contains InterPro entry IPR001236: Lactate/malate
dehydrogenase
Length = 332
Score = 167 bits (405), Expect = 9e-42
Identities = 79/154 (51%), Positives = 101/154 (65%), Gaps = 1/154 (0%)
Frame = -3
Query: 733 VPVKDVKRVIIWGNHSSTQFPDASNAVAIVGGAQKSVSEIINDDAYLKGAFVTTVQKRGA 554
VPV DVK VIIWGNHSS+Q+PD ++A +K V E++ DDA+L G F++TVQ+RGA
Sbjct: 174 VPVSDVKNVIIWGNHSSSQYPDVNHAKVQTSSGEKPVRELVKDDAWLDGEFISTVQQRGA 233
Query: 553 AVIXXXXXXXXXXXXXXXSDHMRDWFLGT-EDRWVSMGVVSDGSYGTPRDVVYSFPVTVT 377
A+I DH+RDW LGT E +VSMGV SDGSY P ++YSFPVT
Sbjct: 234 AIIKARKLSSALSAASSACDHIRDWVLGTPEGTFVSMGVYSDGSYSVPSGLIYSFPVTCR 293
Query: 376 NGKWKIVEGLTISDFARQMLDATGKELVEEKQEA 275
NG W IV+GL I + +R+ +D T +EL EEK A
Sbjct: 294 NGDWSIVQGLPIDEVSRKKMDLTAEELKEEKDLA 327
>At5g43330.1 68418.m05296 malate dehydrogenase, cytosolic, putative
strong similarity to cytosolic malate dehydrogenase (EC
1.1.1.37) SP|O24047 {Mesembryanthemum crystallinum},
SP|O48905 {Medicago sativa}, [Prunus persica]
GI:15982948; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 332
Score = 165 bits (400), Expect = 4e-41
Identities = 78/154 (50%), Positives = 101/154 (65%), Gaps = 1/154 (0%)
Frame = -3
Query: 733 VPVKDVKRVIIWGNHSSTQFPDASNAVAIVGGAQKSVSEIINDDAYLKGAFVTTVQKRGA 554
VPV DVK VIIWGNHSSTQ+PD ++A +K V E++ +D +L G F++TVQ+RGA
Sbjct: 174 VPVSDVKNVIIWGNHSSTQYPDVNHATVKTSVGEKPVRELVKNDEWLNGEFISTVQQRGA 233
Query: 553 AVIXXXXXXXXXXXXXXXSDHMRDWFLGT-EDRWVSMGVVSDGSYGTPRDVVYSFPVTVT 377
A+I DH+RDW +GT E +VSMGV SDGSY P ++YSFPVT
Sbjct: 234 AIIKARKLSSALSAASSACDHIRDWVVGTPEGTFVSMGVYSDGSYNVPAGLIYSFPVTCR 293
Query: 376 NGKWKIVEGLTISDFARQMLDATGKELVEEKQEA 275
NG+W IV+GL I D +R+ +D T +EL EEK A
Sbjct: 294 NGEWTIVQGLPIDDASRKKMDLTAEELKEEKDLA 327
>At5g56720.1 68418.m07079 malate dehydrogenase, cytosolic, putative
similar to cytosolic malate dehydrogenase from
Mesembryanthemum crystallinum [SP|O24047], Medicago
sativa [SP|O48905], Prunus persica [GI:15982948];
contains InterPro entry IPR001236: Lactate/malate
dehydrogenase
Length = 339
Score = 162 bits (393), Expect = 3e-40
Identities = 75/154 (48%), Positives = 98/154 (63%), Gaps = 1/154 (0%)
Frame = -3
Query: 733 VPVKDVKRVIIWGNHSSTQFPDASNAVAIVGGAQKSVSEIINDDAYLKGAFVTTVQKRGA 554
VPV VK VI+WGNHSSTQ+PD ++A + + E++ D +LK F+ VQ+RGA
Sbjct: 180 VPVSSVKNVIVWGNHSSTQYPDTNHATVSTKTGDRPLKELVTDHNWLKNEFIVEVQQRGA 239
Query: 553 AVIXXXXXXXXXXXXXXXSDHMRDWFLGT-EDRWVSMGVVSDGSYGTPRDVVYSFPVTVT 377
AV+ DH+RDWFLGT + WVSMGV SDGSYG P +VYSFPV
Sbjct: 240 AVLRARKQSSAFSAAGAACDHIRDWFLGTPKGTWVSMGVCSDGSYGIPPGLVYSFPVICE 299
Query: 376 NGKWKIVEGLTISDFARQMLDATGKELVEEKQEA 275
G WKIV+GL+I +F+R+ +D + +EL EEK A
Sbjct: 300 KGSWKIVQGLSIDEFSREKMDDSARELAEEKDLA 333
>At5g58330.3 68418.m07302 malate dehydrogenase [NADP], chloroplast,
putative strong similiarity to chloroplast
NADP-dependent malate dehydrogenase (EC 1.1.1.82)
SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum},
SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489
{Flaveria bidentis}, [Flaveria trinervia] GI:726334,
SP|P17606I {Sorghum bicolor}; contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 334
Score = 77.8 bits (183), Expect = 7e-15
Identities = 50/149 (33%), Positives = 76/149 (51%), Gaps = 3/149 (2%)
Frame = -3
Query: 718 VKRVIIWGNHSSTQFPDASNAVAIVGGAQKSVSEIINDDAYLKGAFVTTVQKRGAAVIXX 539
V + IWGNHS+TQ PD N A + G V E+I D +L+ F +VQKRG +I
Sbjct: 164 VSNMTIWGNHSTTQVPDFLN--ARINGL--PVKEVITDHKWLEEGFTESVQKRGGLLIQK 219
Query: 538 XXXXXXXXXXXXXSDHMRDWFLGT-EDRWVSMGVVSDGS-YGTPRDVVYSFPV-TVTNGK 368
D ++ T E W S GV +DG+ YG +V+S P + +G
Sbjct: 220 WGRSSAASTAVSIVDAIKSLVTPTPEGDWFSTGVYTDGNPYGIEEGLVFSMPCRSKGDGD 279
Query: 367 WKIVEGLTISDFARQMLDATGKELVEEKQ 281
+++V+ + I D+ RQ + + EL+ EK+
Sbjct: 280 YELVKDVEIDDYLRQRIAKSEAELLAEKR 308
>At5g58330.2 68418.m07304 malate dehydrogenase [NADP], chloroplast,
putative strong similiarity to chloroplast
NADP-dependent malate dehydrogenase (EC 1.1.1.82)
SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum},
SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489
{Flaveria bidentis}, [Flaveria trinervia] GI:726334,
SP|P17606I {Sorghum bicolor}; contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 442
Score = 77.8 bits (183), Expect = 7e-15
Identities = 50/149 (33%), Positives = 76/149 (51%), Gaps = 3/149 (2%)
Frame = -3
Query: 718 VKRVIIWGNHSSTQFPDASNAVAIVGGAQKSVSEIINDDAYLKGAFVTTVQKRGAAVIXX 539
V + IWGNHS+TQ PD N A + G V E+I D +L+ F +VQKRG +I
Sbjct: 272 VSNMTIWGNHSTTQVPDFLN--ARINGL--PVKEVITDHKWLEEGFTESVQKRGGLLIQK 327
Query: 538 XXXXXXXXXXXXXSDHMRDWFLGT-EDRWVSMGVVSDGS-YGTPRDVVYSFPV-TVTNGK 368
D ++ T E W S GV +DG+ YG +V+S P + +G
Sbjct: 328 WGRSSAASTAVSIVDAIKSLVTPTPEGDWFSTGVYTDGNPYGIEEGLVFSMPCRSKGDGD 387
Query: 367 WKIVEGLTISDFARQMLDATGKELVEEKQ 281
+++V+ + I D+ RQ + + EL+ EK+
Sbjct: 388 YELVKDVEIDDYLRQRIAKSEAELLAEKR 416
>At5g58330.1 68418.m07303 malate dehydrogenase [NADP], chloroplast,
putative strong similiarity to chloroplast
NADP-dependent malate dehydrogenase (EC 1.1.1.82)
SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum},
SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489
{Flaveria bidentis}, [Flaveria trinervia] GI:726334,
SP|P17606I {Sorghum bicolor}; contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 443
Score = 77.8 bits (183), Expect = 7e-15
Identities = 50/149 (33%), Positives = 76/149 (51%), Gaps = 3/149 (2%)
Frame = -3
Query: 718 VKRVIIWGNHSSTQFPDASNAVAIVGGAQKSVSEIINDDAYLKGAFVTTVQKRGAAVIXX 539
V + IWGNHS+TQ PD N A + G V E+I D +L+ F +VQKRG +I
Sbjct: 273 VSNMTIWGNHSTTQVPDFLN--ARINGL--PVKEVITDHKWLEEGFTESVQKRGGLLIQK 328
Query: 538 XXXXXXXXXXXXXSDHMRDWFLGT-EDRWVSMGVVSDGS-YGTPRDVVYSFPV-TVTNGK 368
D ++ T E W S GV +DG+ YG +V+S P + +G
Sbjct: 329 WGRSSAASTAVSIVDAIKSLVTPTPEGDWFSTGVYTDGNPYGIEEGLVFSMPCRSKGDGD 388
Query: 367 WKIVEGLTISDFARQMLDATGKELVEEKQ 281
+++V+ + I D+ RQ + + EL+ EK+
Sbjct: 389 YELVKDVEIDDYLRQRIAKSEAELLAEKR 417
>At4g15920.1 68417.m02418 nodulin MtN3 family protein similar to
MtN3 GI:1619602 (root nodule development) from [Medicago
truncatula]
Length = 241
Score = 27.9 bits (59), Expect = 7.4
Identities = 11/24 (45%), Positives = 17/24 (70%)
Frame = -1
Query: 156 VFFWY*PNHGRLKSIIVKAFINVF 85
+F +Y P H +LK++ V A +NVF
Sbjct: 84 LFLFYAPRHLKLKTVDVDAMLNVF 107
>At3g26855.1 68416.m03360 hypothetical protein
Length = 163
Score = 27.9 bits (59), Expect = 7.4
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1
Query: 418 YHKIHLKQLPC*PICLQCQGTNLS 489
+ +IH ++ PC PICL C LS
Sbjct: 98 FPRIHDERTPCNPICLSCNPMLLS 121
>At3g14840.2 68416.m01875 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain; contains 2 predicted transmembrane domains
Length = 988
Score = 27.9 bits (59), Expect = 7.4
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = -3
Query: 439 VSDGSYGTPRDVVYSFPVTVTNGKWKI 359
+ D + G + VV FPV VTNGK +I
Sbjct: 507 IVDEAKGVGKAVVKKFPVMVTNGKLEI 533
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,965,993
Number of Sequences: 28952
Number of extensions: 310917
Number of successful extensions: 735
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 700
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 726
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1614253080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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