BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10l18f (646 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 39 5e-04 SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch... 32 0.081 SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 28 1.3 SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 28 1.3 SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase |Schizosaccharo... 26 4.0 SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 26 5.3 SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 25 7.1 SPBC216.06c |swi1||replication fork protection complex subunit S... 25 9.3 >SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 330 Score = 39.1 bits (87), Expect = 5e-04 Identities = 29/84 (34%), Positives = 38/84 (45%) Frame = +1 Query: 316 EGVVMELADCALPLLAGVLPTANPEEAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFK 495 EG M+L A PL + KD A + +K G R DLL AN+ IFK Sbjct: 59 EGEAMDLNHAA-PLSHETRVYLGDYKDCKDATAVVITAGKNQKPGETRMDLLKANISIFK 117 Query: 496 EQGQALDKVARKDVKVLVVGNPAN 567 E + + K KD +LV NP + Sbjct: 118 EILREVTKYT-KDAILLVATNPVD 140 >SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 509 Score = 31.9 bits (69), Expect = 0.081 Identities = 14/34 (41%), Positives = 19/34 (55%) Frame = +1 Query: 508 ALDKVARKDVKVLVVGNPANTNALICSKYAPSIP 609 A+ D K +VV + + A +CSKY PSIP Sbjct: 394 AIGASIESDAKAIVVLSTSGNTARLCSKYRPSIP 427 >SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 855 Score = 27.9 bits (59), Expect = 1.3 Identities = 24/62 (38%), Positives = 31/62 (50%), Gaps = 2/62 (3%) Frame = -3 Query: 449 PSFLGIAPTRKAAA--TSLKASSGFAVGRTPAKSGRAQSANSMTTPSSTPIIGAISRRWR 276 P F + P RK +A T+L SS P + RA SANS+T+P P A S + R Sbjct: 516 PQFSAV-PHRKVSAQDTNLMGSSPGMYNHMPYLN-RATSANSITSPGVLPEGMAASLKKR 573 Query: 275 KT 270 T Sbjct: 574 TT 575 >SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 27.9 bits (59), Expect = 1.3 Identities = 23/89 (25%), Positives = 37/89 (41%), Gaps = 5/89 (5%) Frame = -3 Query: 554 PTTRTFTSLRATLSKAWPCSLKMRTLAARRSFLSIPSFLGIAPTRKAA-----ATSLKAS 390 P+ T + L + + P S T A S + S T ++ +T ++ Sbjct: 521 PSYNTSSVLPTSSVSSTPLSSANSTTATSASSTPLTSVNSTTATSASSTPFGNSTITSSA 580 Query: 389 SGFAVGRTPAKSGRAQSANSMTTPSSTPI 303 SG T SG + S+TTP+STP+ Sbjct: 581 SGSTGEFTNTNSGNGDVSGSVTTPTSTPL 609 >SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase |Schizosaccharomyces pombe|chr 1|||Manual Length = 571 Score = 26.2 bits (55), Expect = 4.0 Identities = 8/22 (36%), Positives = 15/22 (68%) Frame = -3 Query: 608 GIDGAYLEHIRAFVLAGLPTTR 543 G+ G+Y EH+ + G+P+T+ Sbjct: 87 GVAGSYAEHVPVVHIVGMPSTK 108 >SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces pombe|chr 1|||Manual Length = 594 Score = 25.8 bits (54), Expect = 5.3 Identities = 10/26 (38%), Positives = 16/26 (61%) Frame = -3 Query: 608 GIDGAYLEHIRAFVLAGLPTTRTFTS 531 GI GAY E++ +++G P T +S Sbjct: 89 GIGGAYAENLPVILVSGSPNTNDLSS 114 >SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1583 Score = 25.4 bits (53), Expect = 7.1 Identities = 14/41 (34%), Positives = 20/41 (48%) Frame = -3 Query: 356 SGRAQSANSMTTPSSTPIIGAISRRWRKTGC*GPKTAPDAI 234 + R+ S N TPSS+P + SR + PK + D I Sbjct: 147 TNRSSSDNGFLTPSSSPRSPSCSRVFNAVQLCSPKKSKDDI 187 >SPBC216.06c |swi1||replication fork protection complex subunit Swi1|Schizosaccharomyces pombe|chr 2|||Manual Length = 971 Score = 25.0 bits (52), Expect = 9.3 Identities = 16/47 (34%), Positives = 27/47 (57%) Frame = -2 Query: 297 RNIKKVEEDRLLRSKNCSRRNLIKK*VCNLSGSTSYNNSDRFSHFDI 157 RNI +++E L++KN + + K + +L T +N D F HFD+ Sbjct: 174 RNILQIDE---LKTKNETIISFAKAHILDLI-VTLVSNLDEFEHFDV 216 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,612,505 Number of Sequences: 5004 Number of extensions: 51756 Number of successful extensions: 172 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 169 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 172 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 289756512 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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