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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV10l18f
         (646 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb...    39   5e-04
SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch...    32   0.081
SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos...    28   1.3  
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce...    28   1.3  
SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase |Schizosaccharo...    26   4.0  
SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces pombe...    26   5.3  
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy...    25   7.1  
SPBC216.06c |swi1||replication fork protection complex subunit S...    25   9.3  

>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 330

 Score = 39.1 bits (87), Expect = 5e-04
 Identities = 29/84 (34%), Positives = 38/84 (45%)
 Frame = +1

Query: 316 EGVVMELADCALPLLAGVLPTANPEEAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFK 495
           EG  M+L   A PL           +  KD  A  +     +K G  R DLL AN+ IFK
Sbjct: 59  EGEAMDLNHAA-PLSHETRVYLGDYKDCKDATAVVITAGKNQKPGETRMDLLKANISIFK 117

Query: 496 EQGQALDKVARKDVKVLVVGNPAN 567
           E  + + K   KD  +LV  NP +
Sbjct: 118 EILREVTKYT-KDAILLVATNPVD 140


>SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 509

 Score = 31.9 bits (69), Expect = 0.081
 Identities = 14/34 (41%), Positives = 19/34 (55%)
 Frame = +1

Query: 508 ALDKVARKDVKVLVVGNPANTNALICSKYAPSIP 609
           A+      D K +VV + +   A +CSKY PSIP
Sbjct: 394 AIGASIESDAKAIVVLSTSGNTARLCSKYRPSIP 427


>SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 855

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 24/62 (38%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
 Frame = -3

Query: 449 PSFLGIAPTRKAAA--TSLKASSGFAVGRTPAKSGRAQSANSMTTPSSTPIIGAISRRWR 276
           P F  + P RK +A  T+L  SS       P  + RA SANS+T+P   P   A S + R
Sbjct: 516 PQFSAV-PHRKVSAQDTNLMGSSPGMYNHMPYLN-RATSANSITSPGVLPEGMAASLKKR 573

Query: 275 KT 270
            T
Sbjct: 574 TT 575


>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1036

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 23/89 (25%), Positives = 37/89 (41%), Gaps = 5/89 (5%)
 Frame = -3

Query: 554 PTTRTFTSLRATLSKAWPCSLKMRTLAARRSFLSIPSFLGIAPTRKAA-----ATSLKAS 390
           P+  T + L  +   + P S    T A   S   + S      T  ++     +T   ++
Sbjct: 521 PSYNTSSVLPTSSVSSTPLSSANSTTATSASSTPLTSVNSTTATSASSTPFGNSTITSSA 580

Query: 389 SGFAVGRTPAKSGRAQSANSMTTPSSTPI 303
           SG     T   SG    + S+TTP+STP+
Sbjct: 581 SGSTGEFTNTNSGNGDVSGSVTTPTSTPL 609


>SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 571

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 8/22 (36%), Positives = 15/22 (68%)
 Frame = -3

Query: 608 GIDGAYLEHIRAFVLAGLPTTR 543
           G+ G+Y EH+    + G+P+T+
Sbjct: 87  GVAGSYAEHVPVVHIVGMPSTK 108


>SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 594

 Score = 25.8 bits (54), Expect = 5.3
 Identities = 10/26 (38%), Positives = 16/26 (61%)
 Frame = -3

Query: 608 GIDGAYLEHIRAFVLAGLPTTRTFTS 531
           GI GAY E++   +++G P T   +S
Sbjct: 89  GIGGAYAENLPVILVSGSPNTNDLSS 114


>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1583

 Score = 25.4 bits (53), Expect = 7.1
 Identities = 14/41 (34%), Positives = 20/41 (48%)
 Frame = -3

Query: 356 SGRAQSANSMTTPSSTPIIGAISRRWRKTGC*GPKTAPDAI 234
           + R+ S N   TPSS+P   + SR +       PK + D I
Sbjct: 147 TNRSSSDNGFLTPSSSPRSPSCSRVFNAVQLCSPKKSKDDI 187


>SPBC216.06c |swi1||replication fork protection complex subunit
           Swi1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 971

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 16/47 (34%), Positives = 27/47 (57%)
 Frame = -2

Query: 297 RNIKKVEEDRLLRSKNCSRRNLIKK*VCNLSGSTSYNNSDRFSHFDI 157
           RNI +++E   L++KN +  +  K  + +L   T  +N D F HFD+
Sbjct: 174 RNILQIDE---LKTKNETIISFAKAHILDLI-VTLVSNLDEFEHFDV 216


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,612,505
Number of Sequences: 5004
Number of extensions: 51756
Number of successful extensions: 172
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 289756512
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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