Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10l18f
(646 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g56720.1 68418.m07079 malate dehydrogenase, cytosolic, putati... 183 8e-47
At1g04410.1 68414.m00432 malate dehydrogenase, cytosolic, putati... 181 4e-46
At5g43330.1 68418.m05296 malate dehydrogenase, cytosolic, putati... 180 6e-46
At5g58330.2 68418.m07304 malate dehydrogenase [NADP], chloroplas... 173 1e-43
At5g58330.1 68418.m07303 malate dehydrogenase [NADP], chloroplas... 173 1e-43
At5g58330.3 68418.m07302 malate dehydrogenase [NADP], chloroplas... 160 8e-40
At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast... 48 4e-06
At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal, puta... 48 5e-06
At3g19170.1 68416.m02434 peptidase M16 family protein / insulina... 29 2.0
At1g49630.3 68414.m05566 peptidase M16 family protein / insulina... 29 2.0
At1g49630.2 68414.m05565 peptidase M16 family protein / insulina... 29 2.0
At1g49630.1 68414.m05564 peptidase M16 family protein / insulina... 29 2.0
At5g25350.1 68418.m03007 F-box family protein contains Pfam PF00... 28 4.6
At2g23160.1 68415.m02767 F-box family protein contains Pfam PF00... 28 4.6
At3g09000.1 68416.m01053 proline-rich family protein 28 6.1
>At5g56720.1 68418.m07079 malate dehydrogenase, cytosolic, putative
similar to cytosolic malate dehydrogenase from
Mesembryanthemum crystallinum [SP|O24047], Medicago
sativa [SP|O48905], Prunus persica [GI:15982948];
contains InterPro entry IPR001236: Lactate/malate
dehydrogenase
Length = 339
Score = 183 bits (446), Expect = 8e-47
Identities = 89/157 (56%), Positives = 109/157 (69%)
Frame = +1
Query: 172 EPIRVVVTGAAGQIAYSLLYQIASGAVFGPQQPVFLHLLDIAPMMGVLEGVVMELADCAL 351
+PIRV++TGAAG I Y++ IA G + GP QP+ LHLLDI P LE V MEL D A
Sbjct: 10 DPIRVLITGAAGNIGYAIAPMIARGIMLGPDQPMILHLLDIEPASSSLEAVKMELQDSAF 69
Query: 352 PLLAGVLPTANPEEAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFKEQGQALDKVARK 531
PLL GV+ T N EA KDV ++G PR GMERKD+++ NV I+K Q AL++ A
Sbjct: 70 PLLKGVIATTNVVEACKDVNIVIMIGGFPRIAGMERKDVMSKNVVIYKAQASALERYASD 129
Query: 532 DVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLD 642
D KVLVV NPANTNALI ++APSIP+EN T +TRLD
Sbjct: 130 DCKVLVVANPANTNALILKEFAPSIPEENITCLTRLD 166
>At1g04410.1 68414.m00432 malate dehydrogenase, cytosolic, putative
strong similarity to malate dehydrogenase from
Mesembryanthemum crystallinum [SP|O24047], Medicago
sativa [SP|O48905], Prunus persica [GI:15982948];
contains InterPro entry IPR001236: Lactate/malate
dehydrogenase
Length = 332
Score = 181 bits (440), Expect = 4e-46
Identities = 89/157 (56%), Positives = 110/157 (70%)
Frame = +1
Query: 172 EPIRVVVTGAAGQIAYSLLYQIASGAVFGPQQPVFLHLLDIAPMMGVLEGVVMELADCAL 351
EP+RV+VTGAAGQI Y+L+ IA G + G QPV LH+LDI P L GV MEL D A
Sbjct: 4 EPVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPPAAEALNGVKMELIDAAF 63
Query: 352 PLLAGVLPTANPEEAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFKEQGQALDKVARK 531
PLL GV+ T + E V A +VG PRKEGMERKD+++ NV I+K Q AL+K A
Sbjct: 64 PLLKGVVATTDAVEGCTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQAAALEKHAAP 123
Query: 532 DVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLD 642
+ KVLVV NPANTNALI ++APSIP++N + +TRLD
Sbjct: 124 NCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLD 160
>At5g43330.1 68418.m05296 malate dehydrogenase, cytosolic, putative
strong similarity to cytosolic malate dehydrogenase (EC
1.1.1.37) SP|O24047 {Mesembryanthemum crystallinum},
SP|O48905 {Medicago sativa}, [Prunus persica]
GI:15982948; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 332
Score = 180 bits (439), Expect = 6e-46
Identities = 90/157 (57%), Positives = 110/157 (70%)
Frame = +1
Query: 172 EPIRVVVTGAAGQIAYSLLYQIASGAVFGPQQPVFLHLLDIAPMMGVLEGVVMELADCAL 351
EP+RV+VTGAAGQI Y+L+ IA G + G QPV LH+LDI L GV MEL D A
Sbjct: 4 EPVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPFAAEALNGVKMELVDAAF 63
Query: 352 PLLAGVLPTANPEEAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFKEQGQALDKVARK 531
PLL GV+ T + EA V A +VG PRKEGMERKD+++ NV I+K Q AL+K A
Sbjct: 64 PLLKGVVATTDAVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKHAAP 123
Query: 532 DVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLD 642
+ KVLVV NPANTNALI ++APSIP++N T +TRLD
Sbjct: 124 NCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLD 160
>At5g58330.2 68418.m07304 malate dehydrogenase [NADP], chloroplast,
putative strong similiarity to chloroplast
NADP-dependent malate dehydrogenase (EC 1.1.1.82)
SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum},
SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489
{Flaveria bidentis}, [Flaveria trinervia] GI:726334,
SP|P17606I {Sorghum bicolor}; contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 442
Score = 173 bits (420), Expect = 1e-43
Identities = 89/156 (57%), Positives = 108/156 (69%)
Frame = +1
Query: 178 IRVVVTGAAGQIAYSLLYQIASGAVFGPQQPVFLHLLDIAPMMGVLEGVVMELADCALPL 357
I + V+GAAG I+ LL+++ASG VFGP QP+ L LL + LEGV MEL D PL
Sbjct: 99 INIAVSGAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSIQALEGVAMELEDSLFPL 158
Query: 358 LAGVLPTANPEEAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFKEQGQALDKVARKDV 537
L V +P E F+DV A L+GA PR GMER DLL N +IF EQG+AL+K A +V
Sbjct: 159 LREVDIGTDPNEVFQDVEWAILIGAKPRGPGMERADLLDINGQIFAEQGKALNKAASPNV 218
Query: 538 KVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQ 645
KVLVVGNP NTNALIC K AP+IP +NF A+TRLD+
Sbjct: 219 KVLVVGNPCNTNALICLKNAPNIPAKNFHALTRLDE 254
>At5g58330.1 68418.m07303 malate dehydrogenase [NADP], chloroplast,
putative strong similiarity to chloroplast
NADP-dependent malate dehydrogenase (EC 1.1.1.82)
SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum},
SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489
{Flaveria bidentis}, [Flaveria trinervia] GI:726334,
SP|P17606I {Sorghum bicolor}; contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 443
Score = 173 bits (420), Expect = 1e-43
Identities = 89/156 (57%), Positives = 108/156 (69%)
Frame = +1
Query: 178 IRVVVTGAAGQIAYSLLYQIASGAVFGPQQPVFLHLLDIAPMMGVLEGVVMELADCALPL 357
I + V+GAAG I+ LL+++ASG VFGP QP+ L LL + LEGV MEL D PL
Sbjct: 100 INIAVSGAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSIQALEGVAMELEDSLFPL 159
Query: 358 LAGVLPTANPEEAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFKEQGQALDKVARKDV 537
L V +P E F+DV A L+GA PR GMER DLL N +IF EQG+AL+K A +V
Sbjct: 160 LREVDIGTDPNEVFQDVEWAILIGAKPRGPGMERADLLDINGQIFAEQGKALNKAASPNV 219
Query: 538 KVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQ 645
KVLVVGNP NTNALIC K AP+IP +NF A+TRLD+
Sbjct: 220 KVLVVGNPCNTNALICLKNAPNIPAKNFHALTRLDE 255
>At5g58330.3 68418.m07302 malate dehydrogenase [NADP], chloroplast,
putative strong similiarity to chloroplast
NADP-dependent malate dehydrogenase (EC 1.1.1.82)
SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum},
SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489
{Flaveria bidentis}, [Flaveria trinervia] GI:726334,
SP|P17606I {Sorghum bicolor}; contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 334
Score = 160 bits (388), Expect = 8e-40
Identities = 83/145 (57%), Positives = 100/145 (68%)
Frame = +1
Query: 211 IAYSLLYQIASGAVFGPQQPVFLHLLDIAPMMGVLEGVVMELADCALPLLAGVLPTANPE 390
I+ LL+++ASG VFGP QP+ L LL + LEGV MEL D PLL V +P
Sbjct: 2 ISNHLLFKLASGEVFGPDQPIALKLLGSERSIQALEGVAMELEDSLFPLLREVDIGTDPN 61
Query: 391 EAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFKEQGQALDKVARKDVKVLVVGNPANT 570
E F+DV A L+GA PR GMER DLL N +IF EQG+AL+K A +VKVLVVGNP NT
Sbjct: 62 EVFQDVEWAILIGAKPRGPGMERADLLDINGQIFAEQGKALNKAASPNVKVLVVGNPCNT 121
Query: 571 NALICSKYAPSIPKENFTAMTRLDQ 645
NALIC K AP+IP +NF A+TRLD+
Sbjct: 122 NALICLKNAPNIPAKNFHALTRLDE 146
>At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast
(MDH) identical to chloroplast NAD-malate dehydrogenase
[Arabidopsis thaliana] GI:3256066; contains InterPro
entry IPR001236: Lactate/malate dehydrogenase; contains
Pfam profiles PF00056: lactate/malate dehydrogenase, NAD
binding domain and PF02866: lactate/malate
dehydrogenase, alpha/beta C-terminal domain
Length = 403
Score = 48.4 bits (110), Expect = 4e-06
Identities = 45/150 (30%), Positives = 65/150 (43%), Gaps = 3/150 (2%)
Frame = +1
Query: 151 YGNIKMAEPIRVVVTGAAGQIAYSLLYQIASGAVFGPQQPVFLHLLDIAPMMGVLEGVVM 330
YG K+ +V V GAAG I L I + LHL DIA + +GV
Sbjct: 75 YG-FKINASYKVAVLGAAGGIGQPLSLLIKMSPLVST-----LHLYDIANV----KGVAA 124
Query: 331 ELADCALPLLAGVLPTANPEE---AFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFKEQ 501
+L+ C P + V P E KDV + +PRK GM R DL N I K
Sbjct: 125 DLSHCNTP--SQVRDFTGPSELADCLKDVNVVVIPAGVPRKPGMTRDDLFNINANIVKTL 182
Query: 502 GQALDKVARKDVKVLVVGNPANTNALICSK 591
+A+ + + + ++ NP N+ I ++
Sbjct: 183 VEAVAENC-PNAFIHIISNPVNSTVPIAAE 211
>At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal,
putative strong similarity to glyoxysomal malate
dehydrogenase (EC 1.1.1.37) SP|P19446 {Citrullus
lanatus}, SP|P46488 {Cucumis sativus}, [Medicago sativa]
GI:2827078, SP|Q42972 {Oryza sativa}, SP|Q9ZP05
{Arabidopsis thaliana}, SP|P37228 {Glycine max};
contains InterPro entry IPR001236: Lactate/malate
dehydrogenase
Length = 354
Score = 48.0 bits (109), Expect = 5e-06
Identities = 43/157 (27%), Positives = 66/157 (42%), Gaps = 3/157 (1%)
Frame = +1
Query: 181 RVVVTGAAGQIAYSLLYQIASGAVFGPQQPVFLHLLDIAPMMGVLEGVVMELADCALPLL 360
+V + GAAG I L + + LHL D+A GV + D + ++
Sbjct: 44 KVAILGAAGGIGQPLAMLMKMNPLVS-----VLHLYDVANAPGVTADI--SHMDTSA-VV 95
Query: 361 AGVLPTANPEEAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFKEQGQALDKVARKDVK 540
G L EEA + + +PRK GM R DL N I + +A+ K K +
Sbjct: 96 RGFLGQPQLEEALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLSEAIAKCCPKAI- 154
Query: 541 VLVVGNPANTNALICS---KYAPSIPKENFTAMTRLD 642
V ++ NP N+ I + K A + + +T LD
Sbjct: 155 VNIISNPVNSTVPIAAEVFKKAGTFDPKKLMGVTMLD 191
>At3g19170.1 68416.m02434 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 1080
Score = 29.5 bits (63), Expect = 2.0
Identities = 14/43 (32%), Positives = 25/43 (58%)
Frame = +1
Query: 421 LVGAMPRKEGMERKDLLAANVRIFKEQGQALDKVARKDVKVLV 549
L+G + +R+++L +++ FK+ QA+D V K V V V
Sbjct: 1015 LLGVTDEERQRKREEILTTSLKDFKDFAQAIDVVRDKGVAVAV 1057
>At1g49630.3 68414.m05566 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 1080
Score = 29.5 bits (63), Expect = 2.0
Identities = 13/43 (30%), Positives = 26/43 (60%)
Frame = +1
Query: 421 LVGAMPRKEGMERKDLLAANVRIFKEQGQALDKVARKDVKVLV 549
L+ + + R+++L+ +++ FKE +A+D V+ K V V V
Sbjct: 1014 LLNVTDEERQIRREEILSTSLKDFKEFAEAIDSVSDKGVAVAV 1056
>At1g49630.2 68414.m05565 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 1080
Score = 29.5 bits (63), Expect = 2.0
Identities = 13/43 (30%), Positives = 26/43 (60%)
Frame = +1
Query: 421 LVGAMPRKEGMERKDLLAANVRIFKEQGQALDKVARKDVKVLV 549
L+ + + R+++L+ +++ FKE +A+D V+ K V V V
Sbjct: 1014 LLNVTDEERQIRREEILSTSLKDFKEFAEAIDSVSDKGVAVAV 1056
>At1g49630.1 68414.m05564 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 1080
Score = 29.5 bits (63), Expect = 2.0
Identities = 13/43 (30%), Positives = 26/43 (60%)
Frame = +1
Query: 421 LVGAMPRKEGMERKDLLAANVRIFKEQGQALDKVARKDVKVLV 549
L+ + + R+++L+ +++ FKE +A+D V+ K V V V
Sbjct: 1014 LLNVTDEERQIRREEILSTSLKDFKEFAEAIDSVSDKGVAVAV 1056
>At5g25350.1 68418.m03007 F-box family protein contains Pfam
PF00646: F-box domain and Pfam PF00560: Leucine Rich
Repeat (6 copies); similar to F-box protein FBL6
(GI:4432860) [Homo sapiens]
Length = 623
Score = 28.3 bits (60), Expect = 4.6
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = +3
Query: 201 CRTNCILTSLSNCVWSSFWTSATCLPPPS*YCAYDGCT*RCCHGVG 338
C + SL+NC+ S + S + LP PS + + RCC G G
Sbjct: 402 CGSKLKAFSLANCLGISDFNSESSLPSPS-CSSLRSLSIRCCPGFG 446
>At2g23160.1 68415.m02767 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640 :
F-box protein interaction domain
Length = 395
Score = 28.3 bits (60), Expect = 4.6
Identities = 20/59 (33%), Positives = 27/59 (45%)
Frame = -3
Query: 539 FTSLRATLSKAWPCSLKMRTLAARRSFLSIPSFLGIAPTRKAAATSLKASSGFAVGRTP 363
FT L T S PC L SF S+P + P K+++ S+ AS+ F V P
Sbjct: 43 FTELFLTRSSPKPCILFATVADGVWSFFSLPQY----PYEKSSSASVAASAKFHVKFPP 97
>At3g09000.1 68416.m01053 proline-rich family protein
Length = 541
Score = 27.9 bits (59), Expect = 6.1
Identities = 23/83 (27%), Positives = 30/83 (36%)
Frame = -3
Query: 554 PTTRTFTSLRATLSKAWPCSLKMRTLAARRSFLSIPSFLGIAPTRKAAATSLKASSGFAV 375
P T T S T S + P + + + A T AA + SSG A
Sbjct: 159 PATPTRRSTTPTTSTSRPVTTRASNSRSSTPTSRATLTAARATTSTAAPRTTTTSSGSAR 218
Query: 374 GRTPAKSGRAQSANSMTTPSSTP 306
TP +S S+ S P S P
Sbjct: 219 SATPTRSNPRPSSASSKKPVSRP 241
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,689,524
Number of Sequences: 28952
Number of extensions: 276412
Number of successful extensions: 804
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 777
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 804
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1334473344
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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