BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10l10f (565 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1F12.07 |||phosphoserine aminotransferase |Schizosaccharomyc... 52 8e-08 SPAC3C7.06c |pit1||serine/threonine protein kinase Pit1|Schizosa... 28 0.82 SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ... 27 2.5 SPCC4B3.03c |||DUF21 domain protein|Schizosaccharomyces pombe|ch... 27 2.5 SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces ... 27 2.5 SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce... 26 4.4 SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 25 5.8 SPBC25D12.06 |||RNA helicase |Schizosaccharomyces pombe|chr 2|||... 25 5.8 SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 25 7.7 >SPAC1F12.07 |||phosphoserine aminotransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 389 Score = 51.6 bits (118), Expect = 8e-08 Identities = 39/162 (24%), Positives = 67/162 (41%), Gaps = 15/162 (9%) Frame = +1 Query: 124 KVFNFGAGPAKLPEEVYEIIKNELTNFENSGISLLETSHRSSTYMKLNVEIQDVVRNLLD 303 +V NF AGPA + V E + NF+ G+ + E SHRS + + R L + Sbjct: 6 EVVNFAAGPAAMITSVVEEFGKDFVNFQGLGMGVAEISHRSKQGSGIVTSAESNFRKLYN 65 Query: 304 VPDNYKVXXXXXXXXXXXXXVPLNLI---------SRTGTADYVVTGAWSXXXXXXXXXY 456 +P+N+ + N+ +++ A+Y++TGAWS Sbjct: 66 IPENFHILFMQGGGTEQFAACLYNVYAHHALKNGNAKSLVANYIITGAWSKKAYAEAERL 125 Query: 457 G-KVNLVLPPTD---KYEDIPD--QTKWNLDPNASYVHICTN 564 G ++ + + KY +P+ K+ D S V+ C N Sbjct: 126 GFPCHVAVDMKELAGKYGSLPEDKDLKFTPDGETSLVYYCDN 167 >SPAC3C7.06c |pit1||serine/threonine protein kinase Pit1|Schizosaccharomyces pombe|chr 1|||Manual Length = 650 Score = 28.3 bits (60), Expect = 0.82 Identities = 13/33 (39%), Positives = 20/33 (60%) Frame = -1 Query: 535 WDQGSILSDQVCLHIYLSVVAPNLLSHIFSPPW 437 WD+ +L+++ L I L +AP +FSPPW Sbjct: 275 WDRAELLANK--LGISLPKMAPLDFGDLFSPPW 305 >SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1502 Score = 26.6 bits (56), Expect = 2.5 Identities = 12/39 (30%), Positives = 21/39 (53%) Frame = +1 Query: 184 KNELTNFENSGISLLETSHRSSTYMKLNVEIQDVVRNLL 300 +N+ + +SLL S S++Y L + D+V N+L Sbjct: 1197 RNQWSQVRALALSLLSDSLNSTSYRLLGISCSDMVSNIL 1235 >SPCC4B3.03c |||DUF21 domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 679 Score = 26.6 bits (56), Expect = 2.5 Identities = 12/45 (26%), Positives = 25/45 (55%) Frame = +1 Query: 184 KNELTNFENSGISLLETSHRSSTYMKLNVEIQDVVRNLLDVPDNY 318 +++ T ++ SG+ L T HR KLN + ++ +LD+ + + Sbjct: 211 ESQSTMYKKSGLKTLVTLHRDLGIDKLNQDEVTIITAVLDLREKH 255 >SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1150 Score = 26.6 bits (56), Expect = 2.5 Identities = 14/42 (33%), Positives = 22/42 (52%) Frame = +1 Query: 163 EEVYEIIKNELTNFENSGISLLETSHRSSTYMKLNVEIQDVV 288 E V + N + + + ++LL T H S T KLNV D++ Sbjct: 604 ESVDSVTCNYVLSSSKNPMNLLFTLHNSITDGKLNVHCNDII 645 >SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 844 Score = 25.8 bits (54), Expect = 4.4 Identities = 13/32 (40%), Positives = 18/32 (56%) Frame = -1 Query: 379 LSLKEQLQIDQDPHQLKTALCSCPVHLISFAQ 284 L+LK+ + I + H L A+CS ISF Q Sbjct: 352 LTLKDNICIIDEAHNLIDAICSMHSSSISFRQ 383 >SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 3699 Score = 25.4 bits (53), Expect = 5.8 Identities = 14/48 (29%), Positives = 23/48 (47%) Frame = +1 Query: 181 IKNELTNFENSGISLLETSHRSSTYMKLNVEIQDVVRNLLDVPDNYKV 324 + EL E S LLE + R S + ++ +++L DVP K+ Sbjct: 2671 VLTELAKHEGSSELLLECAWRISDWSNNRESLEVAIKSLSDVPTPRKL 2718 >SPBC25D12.06 |||RNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 565 Score = 25.4 bits (53), Expect = 5.8 Identities = 17/60 (28%), Positives = 24/60 (40%), Gaps = 6/60 (10%) Frame = -1 Query: 394 YQFWILSLKEQLQIDQDPHQLKTALCSCPVHLISFAQHP------EFRHLASCMLKNDDL 233 YQFWI L + ++ + L P L SF P F +L C+ KN + Sbjct: 168 YQFWIERLLHGITEKEELQHIYKILTLTPASLDSFQNRPPYIGITTFPNLQHCIKKNQPI 227 >SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 435 Score = 25.0 bits (52), Expect = 7.7 Identities = 16/50 (32%), Positives = 23/50 (46%), Gaps = 1/50 (2%) Frame = -1 Query: 349 QDPHQLKTALCS-CPVHLISFAQHPEFRHLASCMLKNDDLFPIN*FQNFQ 203 +DP Q K A+C C SF Q L+ C L+ D + + N+Q Sbjct: 277 RDPGQ-KLAICQKCDFAFCSFCQATWHGDLSPCKLEGDSKKLVEMYLNYQ 325 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,208,969 Number of Sequences: 5004 Number of extensions: 41996 Number of successful extensions: 150 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 141 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 149 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 238029836 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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