SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV10l07r
         (756 letters)

Database: human 
           237,096 sequences; 76,859,062 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY236870-1|AAP41817.1|  701|Homo sapiens MUC19 protein.                39   0.022
AK093065-1|BAC04041.1|  280|Homo sapiens protein ( Homo sapiens ...    38   0.030
X63454-1|CAA45054.1|  198|Homo sapiens hst-2 (FGF-6) protein.          34   0.64 
X57075-3|CAA40360.1|   82|Homo sapiens FGF6 protein.                   34   0.64 
X57075-2|CAA40359.1|  105|Homo sapiens FGF6 protein.                   34   0.64 
X57075-1|CAA40358.1|  115|Homo sapiens FGF6 protein.                   34   0.64 
BC121098-1|AAI21099.1|  208|Homo sapiens fibroblast growth facto...    34   0.64 
BC121097-1|AAI21098.1|  208|Homo sapiens fibroblast growth facto...    34   0.64 
AY581424-1|AAS79318.1|  208|Homo sapiens fibroblast growth facto...    34   0.64 
S83341-1|AAB50777.1|   81|Homo sapiens prion protein protein.          32   2.6  
S80539-1|AAB21334.1|   97|Homo sapiens PrP amyloid protein.            32   2.6  
S79978-1|AAB35416.1|   89|Homo sapiens prion protein protein.          32   2.6  
AY458651-1|AAR21603.1|  277|Homo sapiens prion protein protein.        32   2.6  
AF085477-1|AAC62750.2|  285|Homo sapiens prion protein precursor...    32   2.6  
Z34278-1|CAA84032.1|  279|Homo sapiens mucin protein.                  31   3.4  
Y13247-1|CAA73697.1|  940|Homo sapiens FB19 protein protein.           31   5.9  

>AY236870-1|AAP41817.1|  701|Homo sapiens MUC19 protein.
          Length = 701

 Score = 38.7 bits (86), Expect = 0.022
 Identities = 30/101 (29%), Positives = 43/101 (42%)
 Frame = +3

Query: 363 GLGATMAGSATITGATLGPSATITGLGTMMAGSAMITGETAGFSSTFDCSGSTLSTGVAL 542
           G   T  GS   +  T+ P ++ T   T +A  + +TG T G S        T +TGVAL
Sbjct: 102 GSMTTALGSQLSSSQTVIPGSSGTISHTTVAPGSSVTGTTTGASDDQVTGSKTGTTGVAL 161

Query: 543 AASLMLIRMWTRGVSCPGTRGPTWTGDGTMGASTRAGPTSG 665
           + ++      T   +  G    T  G  T GA+TR     G
Sbjct: 162 STTVAPGSSSTEATTSTGVHRTTVVGQKT-GATTRGSAKQG 201


>AK093065-1|BAC04041.1|  280|Homo sapiens protein ( Homo sapiens
           cDNA FLJ35746 fis, clone TESTI2004399, moderately
           similar to APOMUCIN. ).
          Length = 280

 Score = 38.3 bits (85), Expect = 0.030
 Identities = 29/97 (29%), Positives = 42/97 (43%)
 Frame = +3

Query: 375 TMAGSATITGATLGPSATITGLGTMMAGSAMITGETAGFSSTFDCSGSTLSTGVALAASL 554
           T  GS   +  T+ P ++ T   T +A  + +TG T G S        T +TGVAL+ ++
Sbjct: 3   TALGSQLSSSQTVIPGSSGTISHTTVAPGSSVTGTTTGASDDQVTGSKTGTTGVALSTTV 62

Query: 555 MLIRMWTRGVSCPGTRGPTWTGDGTMGASTRAGPTSG 665
                 T   +  G    T  G  T GA+TR     G
Sbjct: 63  APGSSSTEATTSTGVHRTTVVGQKT-GATTRGSAKQG 98


>X63454-1|CAA45054.1|  198|Homo sapiens hst-2 (FGF-6) protein.
          Length = 198

 Score = 33.9 bits (74), Expect = 0.64
 Identities = 19/64 (29%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
 Frame = +2

Query: 293 ARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRGYTRSLGNNYRARYY-DGRISND 469
           +RG    +S    G  G +  V+W  G+  G+    R Y  ++G  +  +   DGRIS  
Sbjct: 41  SRGWGTLLSRSRAGLAGEIAGVNWESGYLVGIKRQRRLYC-NVGIGFHLQVLPDGRISGT 99

Query: 470 HGRN 481
           H  N
Sbjct: 100 HEEN 103


>X57075-3|CAA40360.1|   82|Homo sapiens FGF6 protein.
          Length = 82

 Score = 33.9 bits (74), Expect = 0.64
 Identities = 19/64 (29%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
 Frame = +2

Query: 293 ARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRGYTRSLGNNYRARYY-DGRISND 469
           +RG    +S    G  G +  V+W  G+  G+    R Y  ++G  +  +   DGRIS  
Sbjct: 18  SRGWGTLLSRSRAGLAGEIAGVNWESGYLVGIKRQRRLYC-NVGIGFHLQVLPDGRISGT 76

Query: 470 HGRN 481
           H  N
Sbjct: 77  HEEN 80


>X57075-2|CAA40359.1|  105|Homo sapiens FGF6 protein.
          Length = 105

 Score = 33.9 bits (74), Expect = 0.64
 Identities = 19/64 (29%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
 Frame = +2

Query: 293 ARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRGYTRSLGNNYRARYY-DGRISND 469
           +RG    +S    G  G +  V+W  G+  G+    R Y  ++G  +  +   DGRIS  
Sbjct: 41  SRGWGTLLSRSRAGLAGEIAGVNWESGYLVGIKRQRRLYC-NVGIGFHLQVLPDGRISGT 99

Query: 470 HGRN 481
           H  N
Sbjct: 100 HEEN 103


>X57075-1|CAA40358.1|  115|Homo sapiens FGF6 protein.
          Length = 115

 Score = 33.9 bits (74), Expect = 0.64
 Identities = 19/64 (29%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
 Frame = +2

Query: 293 ARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRGYTRSLGNNYRARYY-DGRISND 469
           +RG    +S    G  G +  V+W  G+  G+    R Y  ++G  +  +   DGRIS  
Sbjct: 51  SRGWGTLLSRSRAGLAGEIAGVNWESGYLVGIKRQRRLYC-NVGIGFHLQVLPDGRISGT 109

Query: 470 HGRN 481
           H  N
Sbjct: 110 HEEN 113


>BC121098-1|AAI21099.1|  208|Homo sapiens fibroblast growth factor 6
           protein.
          Length = 208

 Score = 33.9 bits (74), Expect = 0.64
 Identities = 19/64 (29%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
 Frame = +2

Query: 293 ARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRGYTRSLGNNYRARYY-DGRISND 469
           +RG    +S    G  G +  V+W  G+  G+    R Y  ++G  +  +   DGRIS  
Sbjct: 51  SRGWGTLLSRSRAGLAGEIAGVNWESGYLVGIKRQRRLYC-NVGIGFHLQVLPDGRISGT 109

Query: 470 HGRN 481
           H  N
Sbjct: 110 HEEN 113


>BC121097-1|AAI21098.1|  208|Homo sapiens fibroblast growth factor 6
           protein.
          Length = 208

 Score = 33.9 bits (74), Expect = 0.64
 Identities = 19/64 (29%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
 Frame = +2

Query: 293 ARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRGYTRSLGNNYRARYY-DGRISND 469
           +RG    +S    G  G +  V+W  G+  G+    R Y  ++G  +  +   DGRIS  
Sbjct: 51  SRGWGTLLSRSRAGLAGEIAGVNWESGYLVGIKRQRRLYC-NVGIGFHLQVLPDGRISGT 109

Query: 470 HGRN 481
           H  N
Sbjct: 110 HEEN 113


>AY581424-1|AAS79318.1|  208|Homo sapiens fibroblast growth factor 6
           protein.
          Length = 208

 Score = 33.9 bits (74), Expect = 0.64
 Identities = 19/64 (29%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
 Frame = +2

Query: 293 ARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRGYTRSLGNNYRARYY-DGRISND 469
           +RG    +S    G  G +  V+W  G+  G+    R Y  ++G  +  +   DGRIS  
Sbjct: 51  SRGWGTLLSRSRAGLAGEIAGVNWESGYLVGIKRQRRLYC-NVGIGFHLQVLPDGRISGT 109

Query: 470 HGRN 481
           H  N
Sbjct: 110 HEEN 113


>S83341-1|AAB50777.1|   81|Homo sapiens prion protein protein.
          Length = 81

 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 7   WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 45



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 15  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 53



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 23  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 61



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 31  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 69



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 39  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 77


>S80539-1|AAB21334.1|   97|Homo sapiens PrP amyloid protein.
          Length = 97

 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 7   WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 45



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 15  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 53



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 23  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 61



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 31  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 69



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 39  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 77



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 47  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 85



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 55  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 93


>S79978-1|AAB35416.1|   89|Homo sapiens prion protein protein.
          Length = 89

 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 7   WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 45



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 15  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 53



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 23  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 61



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 31  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 69



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 39  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 77



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 47  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 85


>AY458651-1|AAR21603.1|  277|Homo sapiens prion protein protein.
          Length = 277

 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 57  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 95



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 65  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 103



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 73  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 111


>AF085477-1|AAC62750.2|  285|Homo sapiens prion protein precursor
           protein.
          Length = 285

 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 57  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 95



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 65  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 103



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 73  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 111



 Score = 31.9 bits (69), Expect = 2.6
 Identities = 13/39 (33%), Positives = 15/39 (38%)
 Frame = +2

Query: 290 WARGHNGRVSHDHGGHQGHVTNVHWARGHNGGVSHDHRG 406
           W + H G     HGG  G      W + H GG    H G
Sbjct: 81  WGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGG 119


>Z34278-1|CAA84032.1|  279|Homo sapiens mucin protein.
          Length = 279

 Score = 31.5 bits (68), Expect = 3.4
 Identities = 27/93 (29%), Positives = 37/93 (39%), Gaps = 1/93 (1%)
 Frame = +3

Query: 387 SATITGATLGPSATITGLGTMMAGSAMITGETAGFSSTFDCSGSTLSTGV-ALAASLMLI 563
           SAT T  T GP  T + + T    SA  T  T+G  +T     +T  T V   + +   I
Sbjct: 122 SATTTSTTSGPGTTPSPVPTTSTTSAPTTSTTSGPGTTPSAVPTTSITSVPTTSTNSAPI 181

Query: 564 RMWTRGVSCPGTRGPTWTGDGTMGASTRAGPTS 662
              T   +     GP  T      AST +  T+
Sbjct: 182 SSTTSATTTSRISGPETTPSPVPTASTTSASTT 214



 Score = 30.3 bits (65), Expect = 7.8
 Identities = 26/93 (27%), Positives = 36/93 (38%), Gaps = 1/93 (1%)
 Frame = +3

Query: 387 SATITGATLGPSATITGLGTMMAGSAMITGETAGFSSTFDCSGSTLSTGVALAASLMLIR 566
           SAT T    GP  T + + T    SA  T  T+G  +T     +T +  V   ++     
Sbjct: 186 SATTTSRISGPETTPSPVPTASTTSASTTSTTSGPGTTPSPVPTTSTISVPTTSTTSAST 245

Query: 567 MWTRGVSCPG-TRGPTWTGDGTMGASTRAGPTS 662
             T   S    T GP  T       ST + PT+
Sbjct: 246 TSTTSASTTSTTSGPGTTPSPVPTTSTTSAPTT 278


>Y13247-1|CAA73697.1|  940|Homo sapiens FB19 protein protein.
          Length = 940

 Score = 30.7 bits (66), Expect = 5.9
 Identities = 21/62 (33%), Positives = 28/62 (45%), Gaps = 3/62 (4%)
 Frame = +2

Query: 299  GHNGRVSHDHGGHQGH-VTNVHWARGHN--GGVSHDHRGYTRSLGNNYRARYYDGRISND 469
            GH       HGG  GH + +V   RGH+  G   H+HRG+          R +DG   + 
Sbjct: 844  GHRPHEGPGHGGPHGHRLHDVPGHRGHDHRGQPPHEHRGHDGPGHGGGGHRGHDG--GHS 901

Query: 470  HG 475
            HG
Sbjct: 902  HG 903


  Database: human
    Posted date:  Oct 23, 2007  1:18 PM
  Number of letters in database: 76,859,062
  Number of sequences in database:  237,096
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 71,712,644
Number of Sequences: 237096
Number of extensions: 1256363
Number of successful extensions: 8194
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 7616
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8131
length of database: 76,859,062
effective HSP length: 88
effective length of database: 55,994,614
effective search space used: 9127122082
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -