Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10l02f
(665 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / sta... 58 5e-09
At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / sta... 56 3e-08
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 53 2e-07
At1g69830.1 68414.m08034 alpha-amylase, putative / 1,4-alpha-D-g... 47 1e-05
At4g25000.1 68417.m03587 alpha-amylase, putative / 1,4-alpha-D-g... 39 0.003
At4g09020.1 68417.m01489 isoamylase, putative / starch debranchi... 35 0.042
At2g39930.1 68415.m04907 isoamylase, putative / starch debranchi... 33 0.13
At1g12950.1 68414.m01504 MATE efflux family protein similar to r... 33 0.23
At1g76130.1 68414.m08841 alpha-amylase, putative / 1,4-alpha-D-g... 31 0.52
At1g71270.1 68414.m08225 Vps52/Sac2 family protein similar to SP... 30 1.6
At5g28300.1 68418.m03436 trihelix DNA-binding protein, putative ... 24 4.2
At5g58470.2 68418.m07323 zinc finger (Ran-binding) family protei... 28 6.4
At5g58470.1 68418.m07322 zinc finger (Ran-binding) family protei... 28 6.4
At1g78240.1 68414.m09118 dehydration-responsive protein-related ... 28 6.4
At1g03310.2 68414.m00310 isoamylase, putative / starch debranchi... 28 6.4
At1g03310.1 68414.m00309 isoamylase, putative / starch debranchi... 28 6.4
At2g31580.1 68415.m03858 expressed protein contains Pfam profi... 27 8.5
>At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / starch
branching enzyme class II (SBE2-1) nearly identical to
starch branching enzyme class II [Arabidopsis thaliana]
GI:619939
Length = 858
Score = 58.0 bits (134), Expect = 5e-09
Identities = 28/66 (42%), Positives = 41/66 (62%), Gaps = 1/66 (1%)
Frame = +1
Query: 277 GVDAIWMSPIFESPM-VDFGYDISNFYDIHYEYGTMEDFEELLDKAHELGLKVLLDFVPN 453
G +A+ + I E FGY ++NF+ +GT +D + L+DKAHELGL VL+D V +
Sbjct: 360 GYNAVQIMAIQEHAYYASFGYHVTNFFAPSSRFGTPDDLKSLIDKAHELGLVVLMDIVHS 419
Query: 454 HASNES 471
HAS +
Sbjct: 420 HASKNT 425
>At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / starch
branching enzyme class II (SBE2-2) identical to starch
branching enzyme class II [Arabidopsis thaliana]
GI:726490
Length = 716
Score = 55.6 bits (128), Expect = 3e-08
Identities = 28/66 (42%), Positives = 41/66 (62%), Gaps = 1/66 (1%)
Frame = +1
Query: 277 GVDAIWMSPIFE-SPMVDFGYDISNFYDIHYEYGTMEDFEELLDKAHELGLKVLLDFVPN 453
G +A+ + I E S FGY ++NF+ GT E+ + L+D+AHELGL VL+D V +
Sbjct: 236 GYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRCGTPEELKSLIDRAHELGLVVLMDIVHS 295
Query: 454 HASNES 471
HAS +
Sbjct: 296 HASKNT 301
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 52.8 bits (121), Expect = 2e-07
Identities = 28/70 (40%), Positives = 41/70 (58%), Gaps = 1/70 (1%)
Frame = +1
Query: 256 LKHFVDAGVDAIWMSPIFE-SPMVDFGYDISNFYDIHYEYGTMEDFEELLDKAHELGLKV 432
L H AG +AI + + E GY ++NF+ YGT +DF+ L+D+AH LGL V
Sbjct: 272 LPHVKRAGYNAIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTPDDFKRLVDEAHGLGLLV 331
Query: 433 LLDFVPNHAS 462
LD V ++A+
Sbjct: 332 FLDIVHSYAA 341
>At1g69830.1 68414.m08034 alpha-amylase, putative /
1,4-alpha-D-glucan glucanohydrolase, putative similar to
SP|P17859 Alpha-amylase precursor (EC 3.2.1.1)
(1,4-alpha-D-glucan glucanohydrolase) {Vigna mungo},
alpha-amylase [Malus x domestica] GI:7532799; contains
Pfam profile PF00128: Alpha amylase, catalytic domain
Length = 887
Score = 46.8 bits (106), Expect = 1e-05
Identities = 21/60 (35%), Positives = 37/60 (61%)
Frame = +1
Query: 277 GVDAIWMSPIFESPMVDFGYDISNFYDIHYEYGTMEDFEELLDKAHELGLKVLLDFVPNH 456
G +W+ P ES + GY + Y+++ YGT+++ ++ + K H++G+KVL D V NH
Sbjct: 527 GFTVLWLPPPTESVSPE-GYMPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNH 585
>At4g25000.1 68417.m03587 alpha-amylase, putative /
1,4-alpha-D-glucan glucanohydrolase, putative similar to
alpha-amylase from Vigna mungo SP|P17859, Ipomoea nil
GI:21670851; contains Pfam profile PF00128: Alpha
amylase, catalytic domain
Length = 423
Score = 39.1 bits (87), Expect = 0.003
Identities = 20/67 (29%), Positives = 37/67 (55%), Gaps = 1/67 (1%)
Frame = +1
Query: 271 DAGVDAIWMSPIFESPMVDFGYDISNFYDIHY-EYGTMEDFEELLDKAHELGLKVLLDFV 447
+AG+ +W+ P +S + GY YD++ +YG+ + + L+ ++ G+K L D V
Sbjct: 56 NAGITHLWLPPPSQSVAPE-GYLPGKLYDLNSSKYGSEAELKSLIKALNQKGIKALADIV 114
Query: 448 PNHASNE 468
NH + E
Sbjct: 115 INHRTAE 121
>At4g09020.1 68417.m01489 isoamylase, putative / starch debranching
enzyme, putative similar to isoamylase isoform 3
[Solanum tuberosum] GI:27728149, isoamylase [Oryza
sativa] GI:3252794; contains Pfam profiles PF00128:
Alpha amylase catalytic domain, PF02922: Isoamylase
N-terminal domain
Length = 764
Score = 35.1 bits (77), Expect = 0.042
Identities = 28/96 (29%), Positives = 50/96 (52%), Gaps = 22/96 (22%)
Frame = +1
Query: 253 ELKHFVDAGVDAIWMSPIFE----------SP---MVD-FGYDISNFYDIHYEYGTME-- 384
++ H D G++A+ + P+FE +P MV+ +GY NF+ Y + E
Sbjct: 268 KIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASGEGD 327
Query: 385 ------DFEELLDKAHELGLKVLLDFVPNHASNESE 474
+F+E++ H G++V+LD V NH +NE++
Sbjct: 328 PIKASKEFKEMVKALHSAGIEVILDVVYNH-TNEAD 362
>At2g39930.1 68415.m04907 isoamylase, putative / starch debranching
enzyme, putative similar to isoamylase from [Solanum
tuberosum] GI:27728145, [Triticum aestivum] GI:17932898,
[Hordeum vulgare] GI:21314275, [Oryza sativa]
GI:3252794; contains Pfam profiles PF00128: Alpha
amylase catalytic domain, PF02922: Isoamylase N-terminal
domain
Length = 783
Score = 33.5 bits (73), Expect = 0.13
Identities = 17/56 (30%), Positives = 33/56 (58%)
Frame = +1
Query: 307 FESPMVDFGYDISNFYDIHYEYGTMEDFEELLDKAHELGLKVLLDFVPNHASNESE 474
F SPM+ + SN ++ + +F+ L+ +AH+ G++V++D V NH + +E
Sbjct: 295 FFSPMIRYASASSN----NFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNE 346
>At1g12950.1 68414.m01504 MATE efflux family protein similar to
ripening regulated protein DDTFR18 [Lycopersicon
esculentum] GI:12231296; contains Pfam profile PF01554:
MatE
Length = 522
Score = 32.7 bits (71), Expect = 0.23
Identities = 14/61 (22%), Positives = 30/61 (49%)
Frame = +1
Query: 10 TAATMRATKVVVLVVGSIALLGLVAGFITWVVLSNQNAPTPPPTEVIQLDWWKNCVLYQI 189
TA +++ ++++ G ++ ++ F TW+V+S + P V+ WW V +
Sbjct: 207 TAKFLQSQSKIMVMAGISGVVLVIHSFFTWLVMSRLHWGLPGLALVLNTSWWVIVVAQLV 266
Query: 190 Y 192
Y
Sbjct: 267 Y 267
>At1g76130.1 68414.m08841 alpha-amylase, putative /
1,4-alpha-D-glucan glucanohydrolase, putative strong
similarity to alpha-amylase GI:7532799 from [Malus x
domestica];contains Pfam profile PF00128: Alpha amylase,
catalytic domain
Length = 413
Score = 31.5 bits (68), Expect = 0.52
Identities = 16/61 (26%), Positives = 30/61 (49%)
Frame = +1
Query: 274 AGVDAIWMSPIFESPMVDFGYDISNFYDIHYEYGTMEDFEELLDKAHELGLKVLLDFVPN 453
+G + W+ P +S + GY + Y ++ YG+ + LL K + ++ + D V N
Sbjct: 55 SGFTSAWLPPPSQS-LAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVIN 113
Query: 454 H 456
H
Sbjct: 114 H 114
>At1g71270.1 68414.m08225 Vps52/Sac2 family protein similar to
SP|P39904 SAC2 protein {Saccharomyces cerevisiae};
contains Pfam profile PF04129: Vps52 / Sac2 family
Length = 707
Score = 29.9 bits (64), Expect = 1.6
Identities = 18/62 (29%), Positives = 36/62 (58%), Gaps = 2/62 (3%)
Frame = -3
Query: 441 VEQNFQTKFMSFI*KLFKVLHSAI--LVVYIVEVTDVVPKVDHGGFEYRRHPNSIHACIY 268
++++ T F +F+ + +L +A+ L++Y +TD + K+D GG R SI + +Y
Sbjct: 641 MDKDIITSFSNFLCGM-DILRAALTQLLLYYTRLTDCIKKID-GGSALNRDLVSIQSIMY 698
Query: 267 EV 262
E+
Sbjct: 699 EI 700
>At5g28300.1 68418.m03436 trihelix DNA-binding protein, putative
similar to GT-2 factor [Arabidopsis thaliana GI:416490
Length = 619
Score = 23.8 bits (49), Expect(2) = 4.2
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = -2
Query: 331 QSRPWGIRISETSK*HPRLHLRSALIR**YLLNHQCHHHH 212
+S P+ + S + H H+ S R +++H HHHH
Sbjct: 32 RSLPFPVSFSSFNTNHQPQHMLSLDSR--KIIHHHHHHHH 69
Score = 23.0 bits (47), Expect(2) = 4.2
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = -2
Query: 235 NHQCHHHHCP 206
NH HHHH P
Sbjct: 93 NHHQHHHHHP 102
>At5g58470.2 68418.m07323 zinc finger (Ran-binding) family protein
weak similarity to SP|Q01844 RNA-binding protein EWS
(EWS oncogene) (Ewing sarcoma breakpoint region 1
protein) {Homo sapiens}; contains Pfam profiles PF00076:
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain),
PF00641: Zn-finger in Ran binding protein and others
Length = 422
Score = 27.9 bits (59), Expect = 6.4
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = +3
Query: 27 GYQGGGSRRRIDRFTGTSCRIHHMG 101
GY GGG RR D ++ R HH G
Sbjct: 392 GYGGGGGDRRRDNYSSGPDRNHHGG 416
>At5g58470.1 68418.m07322 zinc finger (Ran-binding) family protein
weak similarity to SP|Q01844 RNA-binding protein EWS
(EWS oncogene) (Ewing sarcoma breakpoint region 1
protein) {Homo sapiens}; contains Pfam profiles PF00076:
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain),
PF00641: Zn-finger in Ran binding protein and others
Length = 422
Score = 27.9 bits (59), Expect = 6.4
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = +3
Query: 27 GYQGGGSRRRIDRFTGTSCRIHHMG 101
GY GGG RR D ++ R HH G
Sbjct: 392 GYGGGGGDRRRDNYSSGPDRNHHGG 416
>At1g78240.1 68414.m09118 dehydration-responsive protein-related
similar to early-responsive to dehydration stress ERD3
protein [Arabidopsis thaliana] GI:15320410; contains
Pfam profile PF03141: Putative methyltransferase
Length = 684
Score = 27.9 bits (59), Expect = 6.4
Identities = 17/46 (36%), Positives = 22/46 (47%)
Frame = +1
Query: 499 EPGYENFFIWADPLPNPENPGVRLPPSNWVSQFGGSAWEWSEKRQQ 636
+PG +F+W PL NP N L N+V F S W+ QQ
Sbjct: 366 KPG--GYFVWTSPLTNPRNKD-HLKRWNFVHDFAESIC-WTLLNQQ 407
>At1g03310.2 68414.m00310 isoamylase, putative / starch debranching
enzyme, putative similar to isoamylase isoform 2
[Solanum tuberosum] GI:27728147, isoamylase from
[Triticum aestivum] GI:17932898, [Hordeum vulgare]
GI:21314275, [Oryza sativa] GI:3252794; contains Pfam
profiles PF00128: Alpha amylase catalytic domain,
PF02922: Isoamylase N-terminal domain; ESTs gb|H36690,
gb|AA712462, gb|AA651230 and gb|N95932 come from this
gene
Length = 882
Score = 27.9 bits (59), Expect = 6.4
Identities = 23/98 (23%), Positives = 40/98 (40%), Gaps = 15/98 (15%)
Frame = +1
Query: 253 ELKHFVDAGVDAIWMSPIFESPMVDFGYDISNFYDIHYEYGTMEDFEE-------LLDKA 411
++ H G +A+ + PIF Y +F+ YG E ++ K
Sbjct: 396 KVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKL 455
Query: 412 HELGLKVLLDFVPNHAS--------NESEYFIKSEARE 501
H G++VLL+ V H + ++S Y+ K A +
Sbjct: 456 HSEGIEVLLEVVFTHTADSGALRGIDDSSYYYKGRAND 493
>At1g03310.1 68414.m00309 isoamylase, putative / starch debranching
enzyme, putative similar to isoamylase isoform 2
[Solanum tuberosum] GI:27728147, isoamylase from
[Triticum aestivum] GI:17932898, [Hordeum vulgare]
GI:21314275, [Oryza sativa] GI:3252794; contains Pfam
profiles PF00128: Alpha amylase catalytic domain,
PF02922: Isoamylase N-terminal domain; ESTs gb|H36690,
gb|AA712462, gb|AA651230 and gb|N95932 come from this
gene
Length = 882
Score = 27.9 bits (59), Expect = 6.4
Identities = 23/98 (23%), Positives = 40/98 (40%), Gaps = 15/98 (15%)
Frame = +1
Query: 253 ELKHFVDAGVDAIWMSPIFESPMVDFGYDISNFYDIHYEYGTMEDFEE-------LLDKA 411
++ H G +A+ + PIF Y +F+ YG E ++ K
Sbjct: 396 KVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKL 455
Query: 412 HELGLKVLLDFVPNHAS--------NESEYFIKSEARE 501
H G++VLL+ V H + ++S Y+ K A +
Sbjct: 456 HSEGIEVLLEVVFTHTADSGALRGIDDSSYYYKGRAND 493
>At2g31580.1 68415.m03858 expressed protein contains Pfam profile:
PF04446 family of unknown function (DUF549)
Length = 567
Score = 27.5 bits (58), Expect = 8.5
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +1
Query: 340 ISNFYDIHYEYGTMEDFEELLDKAHEL 420
+ F DIH+ YG +++ +L K EL
Sbjct: 375 LEEFEDIHFAYGVSDEYSFVLKKESEL 401
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,829,749
Number of Sequences: 28952
Number of extensions: 314113
Number of successful extensions: 1043
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 999
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1042
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1403159472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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