BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10k16r (414 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 29 0.29 SPAC1639.01c ||SPAC806.09c|GNS1/SUR4 family protein|Schizosaccha... 26 2.0 SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 26 2.0 SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr 1|||... 26 2.7 SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pom... 25 3.5 SPCC584.15c |||arrestin/PY protein 2|Schizosaccharomyces pombe|c... 25 4.7 SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 25 6.1 SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 25 6.1 SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 25 6.1 SPBP4H10.20 |nhm1|DcpS|m7G|Schizosaccharomyces pombe|chr 2|||Manual 25 6.1 >SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 331 Score = 29.1 bits (62), Expect = 0.29 Identities = 15/37 (40%), Positives = 20/37 (54%) Frame = -2 Query: 314 INVNRPSEIAADPVDVEDIAEEPLPEVVILPTPVFPE 204 I+ N P+ A+ P+ E + EEPLP LP PE Sbjct: 80 ISKNEPTSEASKPLLNELVPEEPLPREPPLPNEPVPE 116 >SPAC1639.01c ||SPAC806.09c|GNS1/SUR4 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 328 Score = 26.2 bits (55), Expect = 2.0 Identities = 10/25 (40%), Positives = 13/25 (52%) Frame = +2 Query: 8 HLDHHFCIFIYNYTGTIARISVSWL 82 H HH + YT + R S+SWL Sbjct: 145 HCYHHGATAVLVYTQIVGRTSISWL 169 >SPCC417.08 |tef3||translation elongation factor eEF3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1047 Score = 26.2 bits (55), Expect = 2.0 Identities = 17/47 (36%), Positives = 26/47 (55%), Gaps = 3/47 (6%) Frame = +3 Query: 207 RKYG-SGENNNFGQRLFGNVLN--INGISGNLGRTVHVDDDLDEGSA 338 R+YG G N + L ++N + G +L RTV+V+ D+DE A Sbjct: 461 RRYGLCGPNGSGKSTLMRAIVNGQVEGFPTHL-RTVYVEHDIDESEA 506 >SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 25.8 bits (54), Expect = 2.7 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = -2 Query: 308 VNRPSEIAADPVDVEDIAEEPLPEVVILPTPV 213 ++ P++ A+ V E E+P+P V+ P PV Sbjct: 200 LSSPTQGASSNVTPESPPEKPIPSFVLSPPPV 231 >SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 963 Score = 25.4 bits (53), Expect = 3.5 Identities = 14/50 (28%), Positives = 24/50 (48%) Frame = +3 Query: 129 GTNNYGLDDFGEDRAGELHGFRNVDFRKYGSGENNNFGQRLFGNVLNING 278 GT Y + FG+ + G + DF G +N + ++ GNV ++G Sbjct: 762 GTMPYNNNKFGQPQQGYMSQSGFNDFPPIFGGHSNVYNRQQPGNVSGMSG 811 >SPCC584.15c |||arrestin/PY protein 2|Schizosaccharomyces pombe|chr 3|||Manual Length = 594 Score = 25.0 bits (52), Expect = 4.7 Identities = 12/45 (26%), Positives = 19/45 (42%) Frame = -2 Query: 341 DGTPLVQIIINVNRPSEIAADPVDVEDIAEEPLPEVVILPTPVFP 207 DG P + + P+E P + IA P+P ++ P P Sbjct: 524 DGLPRYEEATRPSSPTESVEIPSNTTTIAPSPVPTIIAPALPSTP 568 >SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 24.6 bits (51), Expect = 6.1 Identities = 11/38 (28%), Positives = 23/38 (60%) Frame = +2 Query: 185 RVQERRFQEIREWGE*QLRAKALRQCPQHQRDQRQSRK 298 R QER ++IRE + + + K L+ + +R +R++ + Sbjct: 582 RKQERELKKIREKEKKRDKKKQLKLAKEEERQRREAER 619 >SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr 1|||Manual Length = 1778 Score = 24.6 bits (51), Expect = 6.1 Identities = 9/16 (56%), Positives = 14/16 (87%) Frame = -2 Query: 296 SEIAADPVDVEDIAEE 249 + +AAD +D+EDI+EE Sbjct: 1084 ASLAADNMDIEDISEE 1099 >SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schizosaccharomyces pombe|chr 2|||Manual Length = 501 Score = 24.6 bits (51), Expect = 6.1 Identities = 11/31 (35%), Positives = 16/31 (51%) Frame = -2 Query: 341 DGTPLVQIIINVNRPSEIAADPVDVEDIAEE 249 D L+Q++ N N PS AA V + E+ Sbjct: 383 DSAQLIQLVENRNNPSVAAASNVAADQSTED 413 >SPBP4H10.20 |nhm1|DcpS|m7G|Schizosaccharomyces pombe|chr 2|||Manual Length = 304 Score = 24.6 bits (51), Expect = 6.1 Identities = 12/37 (32%), Positives = 19/37 (51%) Frame = +3 Query: 303 VHVDDDLDEGSAISAGDSHRGTFNNGRSNRDGLVGVN 413 +HVD + +GSA+ + R++ DGL VN Sbjct: 248 LHVDHETGDGSAVGRAILLDDVIDRLRNSPDGLENVN 284 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,274,056 Number of Sequences: 5004 Number of extensions: 24426 Number of successful extensions: 87 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 82 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 87 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 144287194 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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