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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fmgV10k04f
         (603 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces p...   176   2e-45
SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyc...    66   4e-12
SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces...    58   1e-09
SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog |Schizosacchar...    58   1e-09
SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|...    50   3e-07
SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|c...    50   3e-07
SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|S...    45   1e-05
SPBC16A3.13 |meu7|aah4|alpha-amylase homolog Aah4|Schizosaccharo...    42   7e-05
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch...    41   2e-04
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S...    39   5e-04
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch...    36   0.003
SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca...    35   0.011
SPAC458.06 |||phosphoinositide binding protein|Schizosaccharomyc...    30   0.23 
SPCC1795.08c |||histone acetyltransferase complex subunit |Schiz...    27   1.6  
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M...    26   3.7  
SPBC428.03c |pho4||thiamine-repressible acid phosphatase Pho4|Sc...    25   6.4  
SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch...    25   6.4  
SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ...    25   8.5  
SPBC25D12.04 |suc22||ribonucleotide reductase small subunit Suc2...    25   8.5  

>SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 579

 Score =  176 bits (428), Expect = 2e-45
 Identities = 77/156 (49%), Positives = 109/156 (69%), Gaps = 2/156 (1%)
 Frame = +1

Query: 139 DWWETSILYQIYPRSFADSDGDGIGDLNGITSKLEYIKELGVGAVWLSPIFKSPMVDFGY 318
           +WW  + +YQIYP SF DS+GDG GDL GI SK++Y+K L V ++WL PI+ SP+ D GY
Sbjct: 12  NWWRETSVYQIYPASFKDSNGDGFGDLEGIISKVDYLKALNVESIWLCPIYPSPLKDMGY 71

Query: 319 DIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNESVWFQEALNG--NEKY 492
           D++++ +I   YGT+ED + L+K  +E D+K+V+DLV NHTS++  WF+E+ +   N K 
Sbjct: 72  DVSDYKQIDSRYGTLEDLDRLMKALHERDMKLVMDLVLNHTSDQHEWFKESRSSKTNPKR 131

Query: 493 YNYFVWEDGIIDENGNRQPPNNWLSHFRGSAWEYKE 600
             YF W+    +E G R PPNNW S+F  SAWE+ E
Sbjct: 132 DWYF-WKPARYNEKGERLPPNNWRSYFDTSAWEWDE 166


>SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 581

 Score = 66.1 bits (154), Expect = 4e-12
 Identities = 40/130 (30%), Positives = 71/130 (54%), Gaps = 12/130 (9%)
 Frame = +1

Query: 211 GDLNGITSKLEYIKELGVGAVWLSPIFKSPM---VD----FGYDIANFYEIHHEYGTMED 369
           G  NGI + L+YI+ +G  A+W+SPIF++     +D     GY   N YE++H +GT E+
Sbjct: 66  GTWNGIRNHLDYIQGMGFDAIWISPIFENVEGNDIDGSSYHGYWTTNLYELNHHFGTKEE 125

Query: 370 FEALLKKANELDIKVVLDLVPNHTS----NESVWFQEALNGNE-KYYNYFVWEDGIIDEN 534
           F  L+++ ++ DI ++LD+  N  +     E + F++ +  N+  +++   W D   + N
Sbjct: 126 FMELIQELHKRDIWILLDVAINSMAINGPLEQMSFEKVIPFNDASFFHPHCWVD--YESN 183

Query: 535 GNRQPPNNWL 564
                 N WL
Sbjct: 184 DIESVQNCWL 193


>SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 564

 Score = 58.0 bits (134), Expect = 1e-09
 Identities = 41/136 (30%), Positives = 66/136 (48%), Gaps = 20/136 (14%)
 Frame = +1

Query: 88  LLFVACSGIIIKNGEVQDWWETSILYQIYPRSFADSDGDGI------------GDLNGIT 231
           LLF++ + I + N      W   I+YQI    FA  DG               G   GI 
Sbjct: 8   LLFLSSALIHVANAGSNAEWRKRIIYQILTDRFAVDDGSTDNPCDPDANQYCGGTWKGIE 67

Query: 232 SKLEYIKELGVGAVWLSPIFKSPMVDF--------GYDIANFYEIHHEYGTMEDFEALLK 387
           +KL+YI+++G  A+W+SPI K+   D         GY   ++  ++  +GT +D  +L+ 
Sbjct: 68  NKLDYIEDMGFNAIWISPIDKNIEGDIDGAGYAYHGYWNTDYESLNEHFGTEDDLVSLIT 127

Query: 388 KANELDIKVVLDLVPN 435
            A++  I V+LD + N
Sbjct: 128 AAHKAGIWVMLDSIVN 143


>SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 478

 Score = 58.0 bits (134), Expect = 1e-09
 Identities = 46/155 (29%), Positives = 73/155 (47%), Gaps = 22/155 (14%)
 Frame = +1

Query: 103 CSGIIIKNGEVQDWWETSILYQIYPRSFA-DSDG---DGIGDL------NGITSKLEYIK 252
           CS +  K       W   ++YQ+    FA D D       G+L       GIT  L+YIK
Sbjct: 5   CSILPKKEPSYLKLWRKQVIYQVLTDRFALDEDNFYAKASGNLYLGGTWKGITRNLDYIK 64

Query: 253 ELGVGAVWLSPIFK--SPMVD-----FGYDIANFYEIHHEYGTMEDFEALLKKANELDIK 411
            LG  A+W+SPI K  S   D      GY   +  +++  +GT ED + L+   +E ++ 
Sbjct: 65  SLGCTAIWISPIVKNISETTDCGQAYHGYWAQDMTQLNENFGTEEDLKELVNAIHEKNML 124

Query: 412 VVLDLVPN---HTSNESVWF--QEALNGNEKYYNY 501
            ++D+V N   H  ++ V F   +  N  + Y+N+
Sbjct: 125 CMVDIVVNHMGHAGSKPVNFLLYQPFNSGKYYHNW 159


>SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 491

 Score = 50.0 bits (114), Expect = 3e-07
 Identities = 36/121 (29%), Positives = 59/121 (48%), Gaps = 17/121 (14%)
 Frame = +1

Query: 136 QDWWETSILYQIYPRSFADSD------GDGI----GDLNGITSKLEYIKELGVGAVWLSP 285
           +D W    +YQI    FA  D        G     G   GI  KL+YI+ LG  AVW+SP
Sbjct: 21  KDIWRRQCIYQILTDRFALDDHCTTAPSTGRMYLGGTWRGIIQKLDYIQSLGCTAVWISP 80

Query: 286 IFKSPMVDFGYDIA-------NFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTS 444
           I K+     GY  A       +  +++  +GT +D   L+ + ++ ++  ++D+V NH +
Sbjct: 81  IVKNIEGVTGYGEAYHGYWAEDLTQLNPHFGTKQDLTELVDQLHKRNMLCMIDIVVNHMA 140

Query: 445 N 447
           +
Sbjct: 141 H 141


>SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 625

 Score = 50.0 bits (114), Expect = 3e-07
 Identities = 45/187 (24%), Positives = 84/187 (44%), Gaps = 24/187 (12%)
 Frame = +1

Query: 73  VCLLSLLFVACSGIIIKNGEVQDWWETSILYQIYPRSFA-DSDG-------DGI---GDL 219
           + L SL   A  G+     +  + W   I+YQ+    FA DSD        D     G  
Sbjct: 6   IALFSLF--ALFGLPTSLAKSSEEWRDRIIYQVITDRFAVDSDNTPDCSFDDSSYCGGTW 63

Query: 220 NGITSKLEYIKELGVGAVWLSPIFKSPMVDFGYD--------IANFYEIHHEYGTMEDFE 375
           +GI SKL+YI+ +G  A+W+SP+ K+    +G D          +F +++  +G+ +D  
Sbjct: 64  SGIRSKLDYIQGMGFNAIWISPVEKNLEGSYGSDGEAYHGYWNTDFTQLNEHFGSEDDLI 123

Query: 376 ALLKKANELDIKVVLDLVPNHTS----NESVWFQEALNGNE-KYYNYFVWEDGIIDENGN 540
            L+   +  D+ ++ D + N  +     +++ +   +  N+  Y++ + W D     N N
Sbjct: 124 DLITDMHNRDMWIMFDALANSMAIPGPTDNISYSNLVPFNDSSYFHPYCWID--YGSNNN 181

Query: 541 RQPPNNW 561
               + W
Sbjct: 182 TDIEDCW 188


>SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog
           Mde5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 513

 Score = 44.8 bits (101), Expect = 1e-05
 Identities = 25/83 (30%), Positives = 43/83 (51%), Gaps = 7/83 (8%)
 Frame = +1

Query: 211 GDLNGITSKLEYIKELGVGAVWLSPIFKS-------PMVDFGYDIANFYEIHHEYGTMED 369
           G+  GI  KL+YI+ +G  A+W+SPI K+            GY   + Y ++  +GT +D
Sbjct: 62  GNWRGIIDKLDYIQGMGFTAIWISPIIKNIEGRTKYGEAYHGYWPQDLYTLNPHFGTEQD 121

Query: 370 FEALLKKANELDIKVVLDLVPNH 438
              L    ++  + +++D V NH
Sbjct: 122 LIDLADALHDRGMYLMVDTVVNH 144


>SPBC16A3.13 |meu7|aah4|alpha-amylase homolog
           Aah4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 774

 Score = 41.9 bits (94), Expect = 7e-05
 Identities = 24/87 (27%), Positives = 45/87 (51%), Gaps = 7/87 (8%)
 Frame = +1

Query: 211 GDLNGITSKLEYIKELGVGAVWLSPI---FKSPMVD----FGYDIANFYEIHHEYGTMED 369
           G+  GI   L+YI++LG  A+ +SP+    + P        G+   N+Y ++  +G  ED
Sbjct: 62  GNWQGIVDHLDYIRDLGFTAISISPVVEQLEGPEYHGEAYHGHRPKNYYRLNPHFGNEED 121

Query: 370 FEALLKKANELDIKVVLDLVPNHTSNE 450
            + L    +   + +++D+  NHT +E
Sbjct: 122 LKELSDALHGKGMYLMVDVAINHTISE 148


>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
           Mok13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2358

 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 34/147 (23%), Positives = 66/147 (44%), Gaps = 8/147 (5%)
 Frame = +1

Query: 163 YQIYPRSFADSDGDGIGDLNGITSKLEYIKELGVGAVWL--SPIFKSPMVDFGYDIANFY 336
           Y IY   F +      GD+ G+   L+Y++ +G+  +++  +P    P     Y   ++ 
Sbjct: 90  YDIYETGFRNG-----GDIIGLKDSLDYLEIMGIKVIYIAGTPFLNQPWGADQYSPLDYT 144

Query: 337 EIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNESVWFQEALNGNEKYYNY---FV 507
            + H  GT+  +   +++ +     +VLDL  + T  + + F++ LN    +  +    V
Sbjct: 145 ILDHHSGTIAQWRDTIEEIHRRGFYLVLDLTIS-TLGDLIGFRKYLNSTTPFSLFEHEAV 203

Query: 508 WEDGII--DEN-GNRQPPNNWLSHFRG 579
           W+  +I  D N  N+  P   L  F G
Sbjct: 204 WKSNVIYPDWNFTNKYDPKCELPRFWG 230


>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
           Mok11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2397

 Score = 39.1 bits (87), Expect = 5e-04
 Identities = 25/98 (25%), Positives = 46/98 (46%), Gaps = 2/98 (2%)
 Frame = +1

Query: 139 DWWETSILYQIYPRSFADSDGDGIGDLNGITSKLEYIKELGVGAVWL--SPIFKSPMVDF 312
           D+  T   Y IY   F +      GD+ G+   L+Y++ +GV AV+   +P    P    
Sbjct: 83  DFSGTRFEYDIYETEFRNG-----GDIIGVRQSLDYLQGMGVKAVYFAGTPFVNMPWGAD 137

Query: 313 GYDIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDL 426
            Y   +F  +    GT+ D+   +++ +   + V++DL
Sbjct: 138 QYSPLDFTLLDPHLGTINDWRGTIEEMHSKGMYVIVDL 175


>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
           Mok12|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2352

 Score = 36.3 bits (80), Expect = 0.003
 Identities = 22/93 (23%), Positives = 45/93 (48%), Gaps = 4/93 (4%)
 Frame = +1

Query: 211 GDLNGITS--KLEYIKELGVGAVWLS--PIFKSPMVDFGYDIANFYEIHHEYGTMEDFEA 378
           GD+ G+ +   L+Y++ +G+ AV+++  P    P    GY   +F  +    GT+  +  
Sbjct: 103 GDIVGLVTPRSLDYLESMGIKAVYIAGTPFQNLPWYPDGYSPLDFTLLDKHTGTLNQWHE 162

Query: 379 LLKKANELDIKVVLDLVPNHTSNESVWFQEALN 477
            + K +E    VV+D   +  S  S +   +++
Sbjct: 163 AIMKLHERGFYVVVDFTISTLSELSYFVNSSMS 195


>SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan
           synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 2410

 Score = 34.7 bits (76), Expect = 0.011
 Identities = 17/74 (22%), Positives = 38/74 (51%), Gaps = 2/74 (2%)
 Frame = +1

Query: 211 GDLNGITSKLEYIKELGVGAVWL--SPIFKSPMVDFGYDIANFYEIHHEYGTMEDFEALL 384
           GD+ G+   L+Y++ LG   +++  +P    P     Y   ++  + H  GT++ + + +
Sbjct: 106 GDIVGLELSLDYLEGLGTQGIYIAGTPFVNMPWGADQYSPLDYTILDHHLGTIDQWRSTI 165

Query: 385 KKANELDIKVVLDL 426
              +E  + +V+DL
Sbjct: 166 TAMHERGMYLVVDL 179


>SPAC458.06 |||phosphoinositide binding protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 364

 Score = 30.3 bits (65), Expect = 0.23
 Identities = 13/32 (40%), Positives = 18/32 (56%)
 Frame = +3

Query: 435 SYIKRKCLVPRSSQWQRKILQLFRLGRRNHRR 530
           S  K     PR + W+RK+L+L   G+R H R
Sbjct: 269 SEFKSSTTEPRQTHWKRKLLKLIDSGKRAHWR 300


>SPCC1795.08c |||histone acetyltransferase complex subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 985

 Score = 27.5 bits (58), Expect = 1.6
 Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 1/37 (2%)
 Frame = -2

Query: 284 GESQTAPTPN-SLIYSNFEVIPFKSPIPSPSLSAKDL 177
           G S+  P    SL  S  E+I  KSPIPSP    K++
Sbjct: 118 GVSEETPLKETSLELSEEEIITSKSPIPSPETIHKNI 154


>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 663

 Score = 26.2 bits (55), Expect = 3.7
 Identities = 12/33 (36%), Positives = 19/33 (57%)
 Frame = +1

Query: 277 LSPIFKSPMVDFGYDIANFYEIHHEYGTMEDFE 375
           + P  K  ++D   D+A + EIH E  + E+FE
Sbjct: 591 VDPEDKQAVLDAVEDVAEWLEIHGEDASKEEFE 623


>SPBC428.03c |pho4||thiamine-repressible acid phosphatase
           Pho4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 463

 Score = 25.4 bits (53), Expect = 6.4
 Identities = 12/33 (36%), Positives = 16/33 (48%)
 Frame = +1

Query: 475 NGNEKYYNYFVWEDGIIDENGNRQPPNNWLSHF 573
           N    YY+  V+ED  IDEN       +W + F
Sbjct: 202 NSLAMYYSCPVYEDNNIDENTTEAAHTSWRNVF 234


>SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 425

 Score = 25.4 bits (53), Expect = 6.4
 Identities = 11/31 (35%), Positives = 19/31 (61%)
 Frame = +3

Query: 435 SYIKRKCLVPRSSQWQRKILQLFRLGRRNHR 527
           +++  K +  +S +WQ+   +LFRL  RN R
Sbjct: 77  AHLSSKEIWEKSGRWQKTGSELFRLHDRNDR 107


>SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor,
           zf-fungal binuclear cluster type |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 977

 Score = 25.0 bits (52), Expect = 8.5
 Identities = 16/41 (39%), Positives = 20/41 (48%), Gaps = 3/41 (7%)
 Frame = -2

Query: 290 KIGESQTAPTPNS--LIYSNFEVIPFKSPIPS-PSLSAKDL 177
           K   ++ +  PNS   IYSNF   P  +  PS P  S K L
Sbjct: 302 KFDSNRISQVPNSDYSIYSNFNNFPIANHAPSFPQSSVKKL 342


>SPBC25D12.04 |suc22||ribonucleotide reductase small subunit Suc22
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 391

 Score = 25.0 bits (52), Expect = 8.5
 Identities = 13/36 (36%), Positives = 20/36 (55%), Gaps = 5/36 (13%)
 Frame = +1

Query: 442 SNESVWFQEALNGNEKYY-----NYFVWEDGIIDEN 534
           S + V +   LN +E+Y+      YF   DGI++EN
Sbjct: 106 SKDLVDWDNKLNADERYFISTVLAYFAASDGIVNEN 141


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,734,291
Number of Sequences: 5004
Number of extensions: 61542
Number of successful extensions: 203
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 264253462
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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