BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10k04f (603 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces p... 176 2e-45 SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyc... 66 4e-12 SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces... 58 1e-09 SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog |Schizosacchar... 58 1e-09 SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|... 50 3e-07 SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|c... 50 3e-07 SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|S... 45 1e-05 SPBC16A3.13 |meu7|aah4|alpha-amylase homolog Aah4|Schizosaccharo... 42 7e-05 SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch... 41 2e-04 SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 39 5e-04 SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 36 0.003 SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca... 35 0.011 SPAC458.06 |||phosphoinositide binding protein|Schizosaccharomyc... 30 0.23 SPCC1795.08c |||histone acetyltransferase complex subunit |Schiz... 27 1.6 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 26 3.7 SPBC428.03c |pho4||thiamine-repressible acid phosphatase Pho4|Sc... 25 6.4 SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch... 25 6.4 SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ... 25 8.5 SPBC25D12.04 |suc22||ribonucleotide reductase small subunit Suc2... 25 8.5 >SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 579 Score = 176 bits (428), Expect = 2e-45 Identities = 77/156 (49%), Positives = 109/156 (69%), Gaps = 2/156 (1%) Frame = +1 Query: 139 DWWETSILYQIYPRSFADSDGDGIGDLNGITSKLEYIKELGVGAVWLSPIFKSPMVDFGY 318 +WW + +YQIYP SF DS+GDG GDL GI SK++Y+K L V ++WL PI+ SP+ D GY Sbjct: 12 NWWRETSVYQIYPASFKDSNGDGFGDLEGIISKVDYLKALNVESIWLCPIYPSPLKDMGY 71 Query: 319 DIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNESVWFQEALNG--NEKY 492 D++++ +I YGT+ED + L+K +E D+K+V+DLV NHTS++ WF+E+ + N K Sbjct: 72 DVSDYKQIDSRYGTLEDLDRLMKALHERDMKLVMDLVLNHTSDQHEWFKESRSSKTNPKR 131 Query: 493 YNYFVWEDGIIDENGNRQPPNNWLSHFRGSAWEYKE 600 YF W+ +E G R PPNNW S+F SAWE+ E Sbjct: 132 DWYF-WKPARYNEKGERLPPNNWRSYFDTSAWEWDE 166 >SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyces pombe|chr 1|||Manual Length = 581 Score = 66.1 bits (154), Expect = 4e-12 Identities = 40/130 (30%), Positives = 71/130 (54%), Gaps = 12/130 (9%) Frame = +1 Query: 211 GDLNGITSKLEYIKELGVGAVWLSPIFKSPM---VD----FGYDIANFYEIHHEYGTMED 369 G NGI + L+YI+ +G A+W+SPIF++ +D GY N YE++H +GT E+ Sbjct: 66 GTWNGIRNHLDYIQGMGFDAIWISPIFENVEGNDIDGSSYHGYWTTNLYELNHHFGTKEE 125 Query: 370 FEALLKKANELDIKVVLDLVPNHTS----NESVWFQEALNGNE-KYYNYFVWEDGIIDEN 534 F L+++ ++ DI ++LD+ N + E + F++ + N+ +++ W D + N Sbjct: 126 FMELIQELHKRDIWILLDVAINSMAINGPLEQMSFEKVIPFNDASFFHPHCWVD--YESN 183 Query: 535 GNRQPPNNWL 564 N WL Sbjct: 184 DIESVQNCWL 193 >SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces pombe|chr 3|||Manual Length = 564 Score = 58.0 bits (134), Expect = 1e-09 Identities = 41/136 (30%), Positives = 66/136 (48%), Gaps = 20/136 (14%) Frame = +1 Query: 88 LLFVACSGIIIKNGEVQDWWETSILYQIYPRSFADSDGDGI------------GDLNGIT 231 LLF++ + I + N W I+YQI FA DG G GI Sbjct: 8 LLFLSSALIHVANAGSNAEWRKRIIYQILTDRFAVDDGSTDNPCDPDANQYCGGTWKGIE 67 Query: 232 SKLEYIKELGVGAVWLSPIFKSPMVDF--------GYDIANFYEIHHEYGTMEDFEALLK 387 +KL+YI+++G A+W+SPI K+ D GY ++ ++ +GT +D +L+ Sbjct: 68 NKLDYIEDMGFNAIWISPIDKNIEGDIDGAGYAYHGYWNTDYESLNEHFGTEDDLVSLIT 127 Query: 388 KANELDIKVVLDLVPN 435 A++ I V+LD + N Sbjct: 128 AAHKAGIWVMLDSIVN 143 >SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog |Schizosaccharomyces pombe|chr 3|||Manual Length = 478 Score = 58.0 bits (134), Expect = 1e-09 Identities = 46/155 (29%), Positives = 73/155 (47%), Gaps = 22/155 (14%) Frame = +1 Query: 103 CSGIIIKNGEVQDWWETSILYQIYPRSFA-DSDG---DGIGDL------NGITSKLEYIK 252 CS + K W ++YQ+ FA D D G+L GIT L+YIK Sbjct: 5 CSILPKKEPSYLKLWRKQVIYQVLTDRFALDEDNFYAKASGNLYLGGTWKGITRNLDYIK 64 Query: 253 ELGVGAVWLSPIFK--SPMVD-----FGYDIANFYEIHHEYGTMEDFEALLKKANELDIK 411 LG A+W+SPI K S D GY + +++ +GT ED + L+ +E ++ Sbjct: 65 SLGCTAIWISPIVKNISETTDCGQAYHGYWAQDMTQLNENFGTEEDLKELVNAIHEKNML 124 Query: 412 VVLDLVPN---HTSNESVWF--QEALNGNEKYYNY 501 ++D+V N H ++ V F + N + Y+N+ Sbjct: 125 CMVDIVVNHMGHAGSKPVNFLLYQPFNSGKYYHNW 159 >SPAC27E2.01 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 491 Score = 50.0 bits (114), Expect = 3e-07 Identities = 36/121 (29%), Positives = 59/121 (48%), Gaps = 17/121 (14%) Frame = +1 Query: 136 QDWWETSILYQIYPRSFADSD------GDGI----GDLNGITSKLEYIKELGVGAVWLSP 285 +D W +YQI FA D G G GI KL+YI+ LG AVW+SP Sbjct: 21 KDIWRRQCIYQILTDRFALDDHCTTAPSTGRMYLGGTWRGIIQKLDYIQSLGCTAVWISP 80 Query: 286 IFKSPMVDFGYDIA-------NFYEIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTS 444 I K+ GY A + +++ +GT +D L+ + ++ ++ ++D+V NH + Sbjct: 81 IVKNIEGVTGYGEAYHGYWAEDLTQLNPHFGTKQDLTELVDQLHKRNMLCMIDIVVNHMA 140 Query: 445 N 447 + Sbjct: 141 H 141 >SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr 3|||Manual Length = 625 Score = 50.0 bits (114), Expect = 3e-07 Identities = 45/187 (24%), Positives = 84/187 (44%), Gaps = 24/187 (12%) Frame = +1 Query: 73 VCLLSLLFVACSGIIIKNGEVQDWWETSILYQIYPRSFA-DSDG-------DGI---GDL 219 + L SL A G+ + + W I+YQ+ FA DSD D G Sbjct: 6 IALFSLF--ALFGLPTSLAKSSEEWRDRIIYQVITDRFAVDSDNTPDCSFDDSSYCGGTW 63 Query: 220 NGITSKLEYIKELGVGAVWLSPIFKSPMVDFGYD--------IANFYEIHHEYGTMEDFE 375 +GI SKL+YI+ +G A+W+SP+ K+ +G D +F +++ +G+ +D Sbjct: 64 SGIRSKLDYIQGMGFNAIWISPVEKNLEGSYGSDGEAYHGYWNTDFTQLNEHFGSEDDLI 123 Query: 376 ALLKKANELDIKVVLDLVPNHTS----NESVWFQEALNGNE-KYYNYFVWEDGIIDENGN 540 L+ + D+ ++ D + N + +++ + + N+ Y++ + W D N N Sbjct: 124 DLITDMHNRDMWIMFDALANSMAIPGPTDNISYSNLVPFNDSSYFHPYCWID--YGSNNN 181 Query: 541 RQPPNNW 561 + W Sbjct: 182 TDIEDCW 188 >SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 44.8 bits (101), Expect = 1e-05 Identities = 25/83 (30%), Positives = 43/83 (51%), Gaps = 7/83 (8%) Frame = +1 Query: 211 GDLNGITSKLEYIKELGVGAVWLSPIFKS-------PMVDFGYDIANFYEIHHEYGTMED 369 G+ GI KL+YI+ +G A+W+SPI K+ GY + Y ++ +GT +D Sbjct: 62 GNWRGIIDKLDYIQGMGFTAIWISPIIKNIEGRTKYGEAYHGYWPQDLYTLNPHFGTEQD 121 Query: 370 FEALLKKANELDIKVVLDLVPNH 438 L ++ + +++D V NH Sbjct: 122 LIDLADALHDRGMYLMVDTVVNH 144 >SPBC16A3.13 |meu7|aah4|alpha-amylase homolog Aah4|Schizosaccharomyces pombe|chr 2|||Manual Length = 774 Score = 41.9 bits (94), Expect = 7e-05 Identities = 24/87 (27%), Positives = 45/87 (51%), Gaps = 7/87 (8%) Frame = +1 Query: 211 GDLNGITSKLEYIKELGVGAVWLSPI---FKSPMVD----FGYDIANFYEIHHEYGTMED 369 G+ GI L+YI++LG A+ +SP+ + P G+ N+Y ++ +G ED Sbjct: 62 GNWQGIVDHLDYIRDLGFTAISISPVVEQLEGPEYHGEAYHGHRPKNYYRLNPHFGNEED 121 Query: 370 FEALLKKANELDIKVVLDLVPNHTSNE 450 + L + + +++D+ NHT +E Sbjct: 122 LKELSDALHGKGMYLMVDVAINHTISE 148 >SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosaccharomyces pombe|chr 2|||Manual Length = 2358 Score = 40.7 bits (91), Expect = 2e-04 Identities = 34/147 (23%), Positives = 66/147 (44%), Gaps = 8/147 (5%) Frame = +1 Query: 163 YQIYPRSFADSDGDGIGDLNGITSKLEYIKELGVGAVWL--SPIFKSPMVDFGYDIANFY 336 Y IY F + GD+ G+ L+Y++ +G+ +++ +P P Y ++ Sbjct: 90 YDIYETGFRNG-----GDIIGLKDSLDYLEIMGIKVIYIAGTPFLNQPWGADQYSPLDYT 144 Query: 337 EIHHEYGTMEDFEALLKKANELDIKVVLDLVPNHTSNESVWFQEALNGNEKYYNY---FV 507 + H GT+ + +++ + +VLDL + T + + F++ LN + + V Sbjct: 145 ILDHHSGTIAQWRDTIEEIHRRGFYLVLDLTIS-TLGDLIGFRKYLNSTTPFSLFEHEAV 203 Query: 508 WEDGII--DEN-GNRQPPNNWLSHFRG 579 W+ +I D N N+ P L F G Sbjct: 204 WKSNVIYPDWNFTNKYDPKCELPRFWG 230 >SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|Schizosaccharomyces pombe|chr 1|||Manual Length = 2397 Score = 39.1 bits (87), Expect = 5e-04 Identities = 25/98 (25%), Positives = 46/98 (46%), Gaps = 2/98 (2%) Frame = +1 Query: 139 DWWETSILYQIYPRSFADSDGDGIGDLNGITSKLEYIKELGVGAVWL--SPIFKSPMVDF 312 D+ T Y IY F + GD+ G+ L+Y++ +GV AV+ +P P Sbjct: 83 DFSGTRFEYDIYETEFRNG-----GDIIGVRQSLDYLQGMGVKAVYFAGTPFVNMPWGAD 137 Query: 313 GYDIANFYEIHHEYGTMEDFEALLKKANELDIKVVLDL 426 Y +F + GT+ D+ +++ + + V++DL Sbjct: 138 QYSPLDFTLLDPHLGTINDWRGTIEEMHSKGMYVIVDL 175 >SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosaccharomyces pombe|chr 2|||Manual Length = 2352 Score = 36.3 bits (80), Expect = 0.003 Identities = 22/93 (23%), Positives = 45/93 (48%), Gaps = 4/93 (4%) Frame = +1 Query: 211 GDLNGITS--KLEYIKELGVGAVWLS--PIFKSPMVDFGYDIANFYEIHHEYGTMEDFEA 378 GD+ G+ + L+Y++ +G+ AV+++ P P GY +F + GT+ + Sbjct: 103 GDIVGLVTPRSLDYLESMGIKAVYIAGTPFQNLPWYPDGYSPLDFTLLDKHTGTLNQWHE 162 Query: 379 LLKKANELDIKVVLDLVPNHTSNESVWFQEALN 477 + K +E VV+D + S S + +++ Sbjct: 163 AIMKLHERGFYVVVDFTISTLSELSYFVNSSMS 195 >SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual Length = 2410 Score = 34.7 bits (76), Expect = 0.011 Identities = 17/74 (22%), Positives = 38/74 (51%), Gaps = 2/74 (2%) Frame = +1 Query: 211 GDLNGITSKLEYIKELGVGAVWL--SPIFKSPMVDFGYDIANFYEIHHEYGTMEDFEALL 384 GD+ G+ L+Y++ LG +++ +P P Y ++ + H GT++ + + + Sbjct: 106 GDIVGLELSLDYLEGLGTQGIYIAGTPFVNMPWGADQYSPLDYTILDHHLGTIDQWRSTI 165 Query: 385 KKANELDIKVVLDL 426 +E + +V+DL Sbjct: 166 TAMHERGMYLVVDL 179 >SPAC458.06 |||phosphoinositide binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 364 Score = 30.3 bits (65), Expect = 0.23 Identities = 13/32 (40%), Positives = 18/32 (56%) Frame = +3 Query: 435 SYIKRKCLVPRSSQWQRKILQLFRLGRRNHRR 530 S K PR + W+RK+L+L G+R H R Sbjct: 269 SEFKSSTTEPRQTHWKRKLLKLIDSGKRAHWR 300 >SPCC1795.08c |||histone acetyltransferase complex subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 985 Score = 27.5 bits (58), Expect = 1.6 Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 1/37 (2%) Frame = -2 Query: 284 GESQTAPTPN-SLIYSNFEVIPFKSPIPSPSLSAKDL 177 G S+ P SL S E+I KSPIPSP K++ Sbjct: 118 GVSEETPLKETSLELSEEEIITSKSPIPSPETIHKNI 154 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 26.2 bits (55), Expect = 3.7 Identities = 12/33 (36%), Positives = 19/33 (57%) Frame = +1 Query: 277 LSPIFKSPMVDFGYDIANFYEIHHEYGTMEDFE 375 + P K ++D D+A + EIH E + E+FE Sbjct: 591 VDPEDKQAVLDAVEDVAEWLEIHGEDASKEEFE 623 >SPBC428.03c |pho4||thiamine-repressible acid phosphatase Pho4|Schizosaccharomyces pombe|chr 2|||Manual Length = 463 Score = 25.4 bits (53), Expect = 6.4 Identities = 12/33 (36%), Positives = 16/33 (48%) Frame = +1 Query: 475 NGNEKYYNYFVWEDGIIDENGNRQPPNNWLSHF 573 N YY+ V+ED IDEN +W + F Sbjct: 202 NSLAMYYSCPVYEDNNIDENTTEAAHTSWRNVF 234 >SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 425 Score = 25.4 bits (53), Expect = 6.4 Identities = 11/31 (35%), Positives = 19/31 (61%) Frame = +3 Query: 435 SYIKRKCLVPRSSQWQRKILQLFRLGRRNHR 527 +++ K + +S +WQ+ +LFRL RN R Sbjct: 77 AHLSSKEIWEKSGRWQKTGSELFRLHDRNDR 107 >SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, zf-fungal binuclear cluster type |Schizosaccharomyces pombe|chr 1|||Manual Length = 977 Score = 25.0 bits (52), Expect = 8.5 Identities = 16/41 (39%), Positives = 20/41 (48%), Gaps = 3/41 (7%) Frame = -2 Query: 290 KIGESQTAPTPNS--LIYSNFEVIPFKSPIPS-PSLSAKDL 177 K ++ + PNS IYSNF P + PS P S K L Sbjct: 302 KFDSNRISQVPNSDYSIYSNFNNFPIANHAPSFPQSSVKKL 342 >SPBC25D12.04 |suc22||ribonucleotide reductase small subunit Suc22 |Schizosaccharomyces pombe|chr 2|||Manual Length = 391 Score = 25.0 bits (52), Expect = 8.5 Identities = 13/36 (36%), Positives = 20/36 (55%), Gaps = 5/36 (13%) Frame = +1 Query: 442 SNESVWFQEALNGNEKYY-----NYFVWEDGIIDEN 534 S + V + LN +E+Y+ YF DGI++EN Sbjct: 106 SKDLVDWDNKLNADERYFISTVLAYFAASDGIVNEN 141 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,734,291 Number of Sequences: 5004 Number of extensions: 61542 Number of successful extensions: 203 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 184 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 195 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 264253462 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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