Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fmgV10k01r
(732 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g22200.1 68416.m02801 4-aminobutyrate aminotransferase / gamm... 31 1.0
At4g29220.1 68417.m04180 phosphofructokinase family protein simi... 30 1.8
At2g20050.1 68415.m02343 protein phosphatase 2C, putative / PP2C... 30 1.8
At4g17130.1 68417.m02579 hypothetical protein 29 3.2
At3g05900.1 68416.m00664 neurofilament protein-related similar t... 29 3.2
At1g18590.1 68414.m02318 sulfotransferase family protein similar... 29 3.2
At1g74100.1 68414.m08582 sulfotransferase family protein similar... 29 4.2
At3g29310.1 68416.m03680 calmodulin-binding protein-related 28 5.6
At1g31380.1 68414.m03841 hypothetical protein 28 5.6
At5g04940.2 68418.m00523 SET domain-containing protein (SUVH1) c... 27 9.7
At5g04940.1 68418.m00522 SET domain-containing protein (SUVH1) c... 27 9.7
At1g22180.3 68414.m02773 SEC14 cytosolic factor family protein /... 27 9.7
At1g22180.2 68414.m02774 SEC14 cytosolic factor family protein /... 27 9.7
At1g22180.1 68414.m02772 SEC14 cytosolic factor family protein /... 27 9.7
At1g05660.1 68414.m00587 polygalacturonase, putative / pectinase... 27 9.7
>At3g22200.1 68416.m02801 4-aminobutyrate aminotransferase /
gamma-amino-N-butyrate transaminase / GABA transaminase
/ beta-alanine--oxoglutarate aminotransferase identical
to gamma-aminobutyrate transaminase subunit precursor
[Arabidopsis thaliana] (EC 2.6.1.19) GI:14030435;
contains Pfam profile PF00202: aminotransferase, class
III; identical to cDNA gamma-aminobutyrate transaminase
subunit precursor, nuclear gene for mitochondrial
product GI:14030434
Length = 504
Score = 30.7 bits (66), Expect = 1.0
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = -2
Query: 620 ERQNLVEQLIVNVIDRITSGIQERGLDPIYIEKAEGDYTF 501
E++N V +++ T+G Q LDP+ I K+EG Y +
Sbjct: 43 EKKNTVGSKGHDMLAPFTAGWQSADLDPLVIAKSEGSYVY 82
>At4g29220.1 68417.m04180 phosphofructokinase family protein similar
to phosphofructokinase [Amycolatopsis methanolica]
GI:17432243; contains Pfam profile PF00365:
Phosphofructokinase
Length = 473
Score = 29.9 bits (64), Expect = 1.8
Identities = 20/85 (23%), Positives = 36/85 (42%)
Frame = -2
Query: 575 RITSGIQERGLDPIYIEKAEGDYTFPISDIFSASGTVAHLALEGASNVVVNDVTFNILRS 396
+I IQ+RG++ +YI +G + +A+ G + ND+ I+
Sbjct: 172 KIVDSIQDRGINQVYIIGGDGSQKGAAAIFEEIRKRKLKVAVAGIPKTIDNDIP--IIDR 229
Query: 395 KFSVDLTLPKLSASSVAVNGEATIF 321
F D + + + A + EAT F
Sbjct: 230 SFGFDTAVEEAQRAINAAHVEATSF 254
>At2g20050.1 68415.m02343 protein phosphatase 2C, putative / PP2C,
putative contains PF00481: Protein phosphatase 2C
domain;
Length = 514
Score = 29.9 bits (64), Expect = 1.8
Identities = 23/69 (33%), Positives = 35/69 (50%), Gaps = 5/69 (7%)
Frame = +3
Query: 426 DDNVAGAFESQVGYGARCAEDV--RNWESVIAFGFFDVDWVE---STLLDTGSDAIDDVH 590
DD+ G F+ +GA+C++ V R E+++ G F VD E S L T S +H
Sbjct: 140 DDHFFGVFDGHGEFGAQCSQFVKRRLCENLLRHGRFRVDPAEACNSAFLTTNS----QLH 195
Query: 591 NQLLDQILS 617
L+D +S
Sbjct: 196 ADLVDDSMS 204
>At4g17130.1 68417.m02579 hypothetical protein
Length = 747
Score = 29.1 bits (62), Expect = 3.2
Identities = 13/49 (26%), Positives = 29/49 (59%)
Frame = +3
Query: 33 NIVDDEEDDALNNLPDLVVVSFEKFWKFVIQSVIQPLGYVLAEENNFES 179
+++ EED LN + D +V+ + + ++ + PLG ++AE+ F++
Sbjct: 247 SVLSSEEDKNLN-MVDAIVMDKSIYKQQTAEAFLSPLGPLIAEDEKFDN 294
>At3g05900.1 68416.m00664 neurofilament protein-related similar to
NF-180 (GI:632549) [Petromyzon marinus] similar to
Neurofilament triplet H protein (200 kDa neurofilament
protein) (Neurofilament heavy polypeptide) (NF-H)
(Swiss-Prot:P12036) [Homo sapiens]
Length = 673
Score = 29.1 bits (62), Expect = 3.2
Identities = 18/47 (38%), Positives = 25/47 (53%)
Frame = -2
Query: 578 DRITSGIQERGLDPIYIEKAEGDYTFPISDIFSASGTVAHLALEGAS 438
+ I S IQE DPI ++ EGD ++PI I A H+ E A+
Sbjct: 317 EEILSTIQESNTDPI--KETEGDASYPIDVIEKAITEEKHVVDEPAN 361
>At1g18590.1 68414.m02318 sulfotransferase family protein similar to
SP|P52837 Flavonol 4'-sulfotransferase (EC 2.8.2.-)
(F4-ST) {Flaveria chloraefolia}; contains Pfam profile
PF00685: Sulfotransferase domain
Length = 346
Score = 29.1 bits (62), Expect = 3.2
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +2
Query: 257 WRPDEGLRQQGFHYWLQPI 313
WRP + + G H+WLQP+
Sbjct: 46 WRPKDPFVEYGGHWWLQPL 64
>At1g74100.1 68414.m08582 sulfotransferase family protein similar to
SP|P52837 Flavonol 4'-sulfotransferase (EC 2.8.2.-)
(F4-ST) {Flaveria chloraefolia}; contains Pfam profile
PF00685: Sulfotransferase domain
Length = 338
Score = 28.7 bits (61), Expect = 4.2
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = +2
Query: 257 WRPDEGLRQQGFHYW 301
WRPDE L Q G H+W
Sbjct: 38 WRPDEILTQYGGHWW 52
>At3g29310.1 68416.m03680 calmodulin-binding protein-related
Length = 551
Score = 28.3 bits (60), Expect = 5.6
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = -1
Query: 591 CERHRSHHFRYPRAWTRPNLHRKS 520
C RH++HH P+ T PN KS
Sbjct: 13 CSRHQNHHNNTPQFATSPNCCNKS 36
>At1g31380.1 68414.m03841 hypothetical protein
Length = 175
Score = 28.3 bits (60), Expect = 5.6
Identities = 13/44 (29%), Positives = 23/44 (52%), Gaps = 3/44 (6%)
Frame = +3
Query: 483 EDVRNWESVIAF---GFFDVDWVESTLLDTGSDAIDDVHNQLLD 605
+D+ N S +++ F +DW+E L D G I ++ +L D
Sbjct: 109 DDLANAYSTLSYVTKAGFKLDWLEKDLKDVGETRIQEIEEELKD 152
>At5g04940.2 68418.m00523 SET domain-containing protein (SUVH1)
contains Pfam profiles PF00856: SET domain, PF05033:
Pre-SET motif, PF02182: YDG/SRA domain; identical to
cDNA SUVH1 (SUVH1) GI:13517742
Length = 670
Score = 27.5 bits (58), Expect = 9.7
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = +3
Query: 159 EENNFESGLNIANSKNG 209
E NFESG+N+A+ +NG
Sbjct: 137 ENMNFESGINVADRENG 153
>At5g04940.1 68418.m00522 SET domain-containing protein (SUVH1)
contains Pfam profiles PF00856: SET domain, PF05033:
Pre-SET motif, PF02182: YDG/SRA domain; identical to
cDNA SUVH1 (SUVH1) GI:13517742
Length = 670
Score = 27.5 bits (58), Expect = 9.7
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = +3
Query: 159 EENNFESGLNIANSKNG 209
E NFESG+N+A+ +NG
Sbjct: 137 ENMNFESGINVADRENG 153
>At1g22180.3 68414.m02773 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
profile: PF00650 CRAL/TRIO domain; similar to
polyphosphoinositide binding protein Ssh1p (GI:|2739044)
{Glycine max}; similar to Phosphatidylinositol Transfer
Protein Sec14p (GI:2780955) [Saccharomyces cerevisiae]
Length = 249
Score = 27.5 bits (58), Expect = 9.7
Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = +3
Query: 579 DDVHNQLLDQILSFS-LKVNF*GHSSDAGSESNKY 680
D++ N+LL+ + L+V F G +SDAG KY
Sbjct: 154 DNLSNKLLEDLFDMEQLEVAFGGKNSDAGFNFEKY 188
>At1g22180.2 68414.m02774 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
profile: PF00650 CRAL/TRIO domain; similar to
polyphosphoinositide binding protein Ssh1p (GI:|2739044)
{Glycine max}; similar to Phosphatidylinositol Transfer
Protein Sec14p (GI:2780955) [Saccharomyces cerevisiae]
Length = 314
Score = 27.5 bits (58), Expect = 9.7
Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = +3
Query: 579 DDVHNQLLDQILSFS-LKVNF*GHSSDAGSESNKY 680
D++ N+LL+ + L+V F G +SDAG KY
Sbjct: 219 DNLSNKLLEDLFDMEQLEVAFGGKNSDAGFNFEKY 253
>At1g22180.1 68414.m02772 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
profile: PF00650 CRAL/TRIO domain; similar to
polyphosphoinositide binding protein Ssh1p (GI:|2739044)
{Glycine max}; similar to Phosphatidylinositol Transfer
Protein Sec14p (GI:2780955) [Saccharomyces cerevisiae]
Length = 249
Score = 27.5 bits (58), Expect = 9.7
Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = +3
Query: 579 DDVHNQLLDQILSFS-LKVNF*GHSSDAGSESNKY 680
D++ N+LL+ + L+V F G +SDAG KY
Sbjct: 154 DNLSNKLLEDLFDMEQLEVAFGGKNSDAGFNFEKY 188
>At1g05660.1 68414.m00587 polygalacturonase, putative / pectinase,
putative similar to polygalacturonase 5 [Lycopersicon
esculentum] GI:2459817; contains Pfam profile PF00295:
Glycosyl hydrolases family 28 (polygalacturonases)
Length = 394
Score = 27.5 bits (58), Expect = 9.7
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = -2
Query: 497 ISDIFSASGTVAHLALEGASNVVVNDV 417
IS + S + V H+ L G +NVVV +V
Sbjct: 158 ISGVKSMNSQVTHMTLNGCTNVVVRNV 184
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,806,594
Number of Sequences: 28952
Number of extensions: 266191
Number of successful extensions: 879
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 857
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 878
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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