BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fmgV10j23r (569 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_43840| Best HMM Match : NDK (HMM E-Value=0) 213 9e-56 SB_45281| Best HMM Match : No HMM Matches (HMM E-Value=.) 72 4e-13 SB_49189| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.22 SB_42687| Best HMM Match : UPF0262 (HMM E-Value=2.1) 30 1.2 SB_43132| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.2 SB_25952| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 8.2 >SB_43840| Best HMM Match : NDK (HMM E-Value=0) Length = 786 Score = 213 bits (520), Expect = 9e-56 Identities = 97/148 (65%), Positives = 112/148 (75%) Frame = -2 Query: 487 ERTFIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRPFFPGL 308 ERTF+M+KPD V RGL+G II RFEKKGFKLV +KFV SE+ ++HY LA F+ GL Sbjct: 639 ERTFLMIKPDAVSRGLIGEIISRFEKKGFKLVAMKFVKKSEDHFRKHYESLAKLKFYDGL 698 Query: 307 VKYMSSGPVVPMVWEGLNVVKTGRQMLGATNPADSQPGTIRGDLCIQVGRNIIHGSDSVE 128 KYMS PV MVWEGL VVKT R MLG T+PA S PGTIRGD I +GRNIIHGSD+VE Sbjct: 699 CKYMSQTPVCAMVWEGLGVVKTARVMLGETDPAKSLPGTIRGDFSIHIGRNIIHGSDAVE 758 Query: 127 SAKKEIGLWFTDKEVVGWTPANENWVYE 44 +AK+EI LWF D E+V WTP N W+YE Sbjct: 759 TAKEEIALWFKDDELVDWTPCNNPWMYE 786 Score = 128 bits (310), Expect = 2e-30 Identities = 68/109 (62%), Positives = 78/109 (71%) Frame = -2 Query: 487 ERTFIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRPFFPGL 308 ERTFIMVKPDGVQRGLVG II+RFE+KGFKLV LK V SEE L++HY+DLA PF+PGL Sbjct: 127 ERTFIMVKPDGVQRGLVGEIIKRFEQKGFKLVALKMVQESEEHLKKHYADLAHLPFYPGL 186 Query: 307 VKYMSSGPVVPMVWEGLNVVKTGRQMLGATNPADSQPGTIRGDLCIQVG 161 VK+MSSGPVV M ADS+PGTIRGD C+ +G Sbjct: 187 VKFMSSGPVVAM--------------------ADSKPGTIRGDFCVHIG 215 >SB_45281| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1256 Score = 71.7 bits (168), Expect = 4e-13 Identities = 45/139 (32%), Positives = 73/139 (52%), Gaps = 3/139 (2%) Frame = -2 Query: 487 ERTFIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRPFFPGL 308 + T ++KP+ V+ I +R ++ GFK+ K V S+EL Q Y + + FF GL Sbjct: 673 QSTVAVIKPE-VEPDQRELIKQRIKEAGFKIQLQKEVTLSKELASQFYHEHEGKDFFEGL 731 Query: 307 VKYMSSGPVVPMVWEGLNVVKTGRQMLGATNPADSQ---PGTIRGDLCIQVGRNIIHGSD 137 YMSSGP + MV + V R ++G +P ++ P +IR L V +N++HG Sbjct: 732 TDYMSSGPTMFMVLSKEDAVSGWRSLMGPVDPEQAKEMAPESIRAALGKDVMKNVVHGPS 791 Query: 136 SVESAKKEIGLWFTDKEVV 80 E A K I +F + +++ Sbjct: 792 DPEKAGKVIKEFFPEAKIL 810 Score = 65.7 bits (153), Expect = 3e-11 Identities = 41/142 (28%), Positives = 75/142 (52%), Gaps = 4/142 (2%) Frame = -2 Query: 493 QRERTFIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDLASRPFFP 314 Q +RT +++PD + R +I+ + ++ GF++ K + + E ++ YS+ + FF Sbjct: 536 QLQRTLALIRPDAL-RSRRESIMSKIQEAGFEIAMSKEMHLTREQAEEFYSEHKDQEFFD 594 Query: 313 GLVKYMSSGPVVPMVWEGLNVVKTGRQMLG---ATNPADSQPGTIRGDLCIQVGR-NIIH 146 LV MSSGP++ + + ++ R MLG D P ++R ++ N +H Sbjct: 595 TLVTNMSSGPMMALCLAREDAIEGWRGMLGPKEVEKAKDEAPESLRAQFQVEDSPINPLH 654 Query: 145 GSDSVESAKKEIGLWFTDKEVV 80 GSD+ E+A+KEI +F + V Sbjct: 655 GSDTAENAEKEIQKFFPMQSTV 676 Score = 28.7 bits (61), Expect = 3.5 Identities = 11/22 (50%), Positives = 15/22 (68%) Frame = -2 Query: 157 NIIHGSDSVESAKKEIGLWFTD 92 N +HG DS ESA +E+ +F D Sbjct: 501 NAVHGCDSNESAARELAFFFPD 522 >SB_49189| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1373 Score = 32.7 bits (71), Expect = 0.22 Identities = 15/31 (48%), Positives = 19/31 (61%) Frame = +3 Query: 237 RPVFTTLRPSHTIGTTGPELMYFTRPGKKGR 329 R VF T+ HT+ T G E +YF RP K+ R Sbjct: 194 RAVFNTMVVLHTVLTKGEETLYFERPIKRPR 224 >SB_42687| Best HMM Match : UPF0262 (HMM E-Value=2.1) Length = 908 Score = 30.3 bits (65), Expect = 1.2 Identities = 13/31 (41%), Positives = 19/31 (61%) Frame = +3 Query: 237 RPVFTTLRPSHTIGTTGPELMYFTRPGKKGR 329 R VF T+ HT+ T G + +YF RP ++ R Sbjct: 759 RAVFNTMVVLHTVLTKGEQTLYFERPIRRPR 789 >SB_43132| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 190 Score = 30.3 bits (65), Expect = 1.2 Identities = 13/31 (41%), Positives = 19/31 (61%) Frame = +3 Query: 237 RPVFTTLRPSHTIGTTGPELMYFTRPGKKGR 329 R VF T+ HT+ T G + +YF RP ++ R Sbjct: 46 RAVFNTMVVLHTVLTKGEQTLYFERPIRRPR 76 >SB_25952| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 195 Score = 27.5 bits (58), Expect = 8.2 Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 3/39 (7%) Frame = +2 Query: 443 TTLYTIRLNHNKST---LTLFRHHEILLTCSINYTCTTA 550 TT YT+ ++ +T T H TC+ +YTCTT+ Sbjct: 149 TTSYTLTTSYTCTTSYTCTTSYTHTTSYTCTTSYTCTTS 187 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,788,804 Number of Sequences: 59808 Number of extensions: 374255 Number of successful extensions: 872 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 802 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 869 length of database: 16,821,457 effective HSP length: 78 effective length of database: 12,156,433 effective search space used: 1349364063 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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